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Human HLA-A*11:01:01 cDNA

Human HLA-A*11:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02869
Resource name Human HLA-A*11:01:01 cDNA
Alternative name HLA-A*11011
Clone info. Human HLA-A*11:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human major histocompatibility complex, class I, A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB02869 Human HLA-A*11:01:01 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02869z.seq
          Remarks, protocol and/or map (gif) RDB02869.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14005_A6D4p1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert5'
          Sequence file: RDB14005_A6D4d.seq check
          >02869_14005_A6D4_CMV-Forward_A10_01.ab1
              1 CGTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
             61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
            121 TCACTATAGG GAGACCCAAG CTGGCTAGCG TTTAAACTTA AGCTTGATTC TCCCCAGACG
            181 CCGAGGATGG CCGTCATGGC GCCCCGAACC CTCCTCCTGC TACTCTCGGG GGCCCTGGCC
            241 CTGACCCAGA CCTGGGCGGG CTCCCACTCC ATGAGGTATT TCTACACCTC CGTGTCCCGG
            301 CCCGGCCGCG GGGAGCCCCG CTTCATCGCC GTGGGCTACG TGGACGACAC GCAGTTCGTG
            361 CGGTTCGACA GCGACGCCGC GAGCCAGAGG ATGGAGCCGC GGGCGCCGTG GATAGAGCAG
            421 GAGGGGCCGG AGTATTGGGA CCAGGAGACA CGGAATGTGA AGGCCCAGTC ACAGACTGAC
            481 CGAGTGGACC TGGGGACCCT GCGCGGCTAC TACAACCAGA GCGAGGACGG TTCTCACACC
            541 ATCCAGATAA TGTATGGCTG CGACGTGGGG CCGGACGGGC GCTTCCTCCG CGGGTACCGG
            601 CAGGACGCCT ACGACGGCAA GGATTACATC GCCCTGAACG AGGACCTGCG CTCTTGGACC
            661 GCGGCGGACA TGGCAGCTCA GATCACCAAG CGCAAGTGGG AGGCGGCCCA TGCGGCGGAG
            721 CAGCAGAGAG CCTACCTGGA GGGCCGGTGC GTGGAGTGGC TCCGCAGATA CCTGGAGAAC
            781 GGGAAGGAGA CGCTGCAGCG CACGGACCCC CCCAAGACAC ATATGACCCA CCACCCCATC
            841 TCTGACCATG AGGCCACCCT GAGGTGCTGG GCCCTGGGCT TCTACCCTGC GGAGATCACA
            901 CTGACCTGGC AGCGGGATGG GGGAGGACCA GACCCAGGAC ACGGAGCTCG TGGAGACCAG
            961 GCCTGCAGGG GATGGAACCT TCCAGAAGTG GGCGGCTGTG GTGGTGCCTT TCTGGAGAGG
           1021 AGCAGAGATA CACCTGCCCA TGTGCAGCCA TGAGGGTCTG CCAGCCCCTC ACCCTGAGAT
          1081 GGGAGCTGTC GTCCAGCCAA CCAATCCCCA TCGGTGGGGC CATCAATTGG CTTGGGGC
          //
          Primer: BGH_rev2
          Region: bGHp(A),insert3'
          Sequence file: RDB14005_A6D4e.seq check
          >02869_14005_A6D4_BGH_rev2_B10_04.ab1
              1 TATCGGGTAG GTAGCACGGG GGAGGGGCAA ACAACAGATG GCTGGCAACT AGAAGGCACA
             61 GTCGAGGCTG ATCAGCGGGT TTAAACGGGC CCTCTAGACT CGAGCGGCCG CACAAGGCAG
            121 CTGTCTCACA CTTTACAAGC TGTGAGAGAC ACATCAGAGC CCTGGGCACT GTCACTGCTT
            181 GCAGCCTGAG TGTAACTCCC TCCTTTTCTA TCTGAGCTCT TCCTCCTCCA CATCACGGCA
            241 GCGACCACAG CTCCAGTGAT CACAGCTCCA AGGAGAACCA GGCCAGCAAT GATGCCCACG
            301 ATGGGGATGG TGGGCTGGGA AGACAGCTCC CATCTCAGGG TGAGGGGCTT GGGCAGACCC
            361 TCATGCTGCA CATGGCAGGT GTATCTCTGC TCCTCTCCAG AAGGCACCAC CACAGCCGCC
            421 CACTTCTGGA AGGTTCCATC CCCTGCAGGC CTGGTCTCCA CGAGCTCCGT GTCCTGGGTC
            481 TGGTCCTCCC CATCCCGCTG CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCCCAGGGCC
            541 CAGCACCTCA GGGTGGCCTC ATGGTCAGAG ATGGGGTGGT GGGTCATATG TGTCTTGGGG
            601 GGGTCCGTGC GCTGCAGCGT CTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CCACTCCACG
            661 CACCGGCCCT CCAGGTAGGC TCTCTGCTGC TCCGCCGCAT GGGCCGCCTC CCACTTGCGC
            721 TTGGTGATCT GAGCTGCCAT GTCCGCCGCG GTCCAAGAGC GCAGGTCCTC GTTCAGGGCG
            781 ATGTAATCCT TGCCGTCGTA GGCGTCCTGC CGGTACCCGC GGAGGAAGCG CCCGTCCGGC
            841 CCCACGTCGC AGCCATACAT TATCTGGATG GTGTGAGAAC CCGTCCTCGC TCTGGTTGTA
            901 GTAGCCGCGC AGGGTCCCCA GGTCCACTCG GTCAGTCTGT GACTGGGCCC TTCACATTCC
            961 GTGTCTCCTG GTCCCAATAC TCCGGGCCCC TTCCTGCTCT ATCCACGGCG CCCGCGGCTC
           1021 CAATCCCTCT GGCTCGCGGC GTCGCTGTCG AACCGGCACG GAACTGCGTG TCGGTCACGT
           1081 AGCCCCACGG CGATGAAGCG GGGCTCCCCG CCGGTCGACT GGGGATCACG GAAGTGTAAA
          1141 ATACCTTCAA TGTGCGAAGA TGATG
          //
          Primer: SV40pro_F_V2
          Region: NeoR
          Sequence file: RDB14005_A6D4f.seq check
          >02869_14005_A6D4_SV40pro_F_V2_C10_07.ab1
              1 TGGTGGGGGG TTTTTTGGGA GGCTAGGCTT TTGCAAAAGC TCCCGGGAGC TTGTATATCC
             61 ATTTTCGGAT CTGATCAAGA GACAGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA
            121 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA
            181 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT
            241 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG
            301 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA
            361 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC
            421 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT
            481 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT
            541 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG
            601 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG
            661 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC
            721 ATCGACTGTG GCCGGCTGGG TGTGGCGGAC CGCTATCAGG ACATAGCGTT GGCTACCCGT
            781 GATATTGCTG AAGAGCTTGG CGGCGAATGG GCTGACCGCT TCCTCGTGCT TTACGGTATC
            841 GCCGCTCCCG ATTCGCAGCG CATCGCCCTT CTATCGCCCT TCTTGACGAG TTCTTCTGAG
            901 CGGGACTCTG GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT
            961 TCGATTCCAC CGCCGCCTTC TATGAAAGGT TGGGCTTCGG GAAATCGTTT TTCCCGGGAC
           1021 GCCCGGCTGG GAATGAATCC TCCCAGCGCG GGGGATCTCA TGCTTGGGAG TTTCTTTCGC
           1081 CCACCCCAAC TTGTTTATTG GCAGCCTTAT AATGGGCTAC GAAATAAGCA AGAGCCATTC
          1141 CACCCAGAAA TTTTCTCACA CCAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl