Resource data sheet
Prev.

Human HLA-A*02:07 cDNA

Human HLA-A*02:07 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02868
Resource name Human HLA-A*02:07 cDNA
Alternative name HLA-A*0207
Clone info. Human HLA-A*02:07 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02868 Human HLA-A*02:07 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02868z.seq from Depositor
Electronic file Map RDB02868.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB02868_A2K5p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB02868_A2K5a.seq
>02868_02868_A2K5_T7_20bp_G03_21.ab1
    1 CATTACCGGC CGCTTTACTT TAAGCTTGAT TCTCCCCAGA CGCCGAGGAT GGCCGTCATG
   61 GCGCCCCGAA CCCTCGTCCT GCTACTCTCG GGGGCTCTGG CCCTGACCCA GACCTGGGCG
  121 GGCTCTCACT CCATGAGGTA TTTCTTCACA TCCGTGTCCC GGCCCGGCCG CGGGGAGCCC
  181 CGCTTCATCG CAGTGGGCTA CGTGGACGAC ACGCAGTTCG TGCGGTTCGA CAGCGACGCC
  241 GCGAGCCAGA GGATGGAGCC GCGGGCGCCG TGGATAGAGC AGGAGGGTCC GGAGTATTGG
  301 GACGGGGAGA CACGGAAAGT GAAGGCCCAC TCACAGACTC ACCGAGTGGA CCTGGGGACC
  361 CTGCGCGGCT ACTACAACCA GAGCGAGGCC GGTTCTCACA CCGTCCAGAG GATGTGTGGC
  421 TGCGACGTGG GGTCGGACTG GCGCTTCCTC CGCGGGTACC ACCAGTACGC CTACGACGGC
  481 AAGGATTACA TCGCCCTGAA AGAGGACCTG CGCTCTTGGA CCGCGGCGGA CATGGCAGCT
  541 CAGACCACCA AGCACAAGTG GGAGGCGGCC CATGTGGCGG AGCAGTTGAG AGCCTACCTG
  601 GAGGGCACGT GCGTGGAGTG GCTCCGCAGA TACCTGGAGA ACGGGAAGGA GACGCTGCAG
  661 CGCACGGACG CCCCCAAAAC GCATATGACT CACCACGCTG TCTCTGACCA TGAAGCCACC
  721 CTGAGGTGCT GGGCCCTGAG CTTCTACCCT GCGGAGATCA CACTGACCTG GCAGCGGGAT
  781 GGGGAGGACC AGACCCAGGA CACGGAGCTC GTGGAGACCA GGCCTGCAGG GGATGGAACC
  841 TTCCAGAAGT GGGCGGCTGT GGTGGTGCCT TCTGGACAGG AGCAGAGATA CACCTGCCAT
  901 GTGCAGCATG AGGGTTTGCC CAAGCCCCTC ACCCTGAGAT GGGAGCCGTC TTCCCAGCCC
  961 ACCATCCCCA TCGTGGGCAT CATTGCTGGC CTGGTTCTCT TTGGGAGCTG TGATCACTGG
 1021 AGCTGTGGGT CGCTGCTGTG ATGTGGAGGA GGAAGAGCTC AGATAGAAAA GGAGGGAGCT
 1081 ACTCTCAGCT GCAGCAGTGA CAGTGCCCAA GGCTCTGGAT GTGTCTCTCA CAAGCCTTGT
1141 AAAGGTGGTG GAGAACACAA GCTTGCCCTG GT
//
Primer: BGH_rev RDB02868_A2K5b.seq
>02868_02868_A2K5_BGHrev_H03_24.ab1
    1 GGTTTTCCGG GGGCCTCTAG ACTCGAGCGG CCGCACAAGG CAGCTGTCTC ACACTTTACA
   61 AGCTGTGAGA GACACATCAG AGCCCTGGGC ACTGTCACTG CTTGCAGCCT GAGAGTAGCT
  121 CCCTCCTTTT CTATCTGAGC TCTTCCTCCT CCACATCACA GCAGCGACCA CAGCTCCAGT
  181 GATCACAGCT CCAAAGAGAA CCAGGCCAGC AATGATGCCC ACGATGGGGA TGGTGGGCTG
  241 GGAAGACGGC TCCCATCTCA GGGTGAGGGG CTTGGGCAAA CCCTCATGCT GCACATGGCA
  301 GGTGTATCTC TGCTCCTGTC CAGAAGGCAC CACCACAGCC GCCCACTTCT GGAAGGTTCC
  361 ATCCCCTGCA GGCCTGGTCT CCACGAGCTC CGTGTCCTGG GTCTGGTCCT CCCCATCCCG
  421 CTGCCAGGTC AGTGTGATCT CCGCAGGGTA GAAGCTCAGG GCCCAGCACC TCAGGGTGGC
  481 TTCATGGTCA GAGACAGCGT GGTGAGTCAT ATGCGTTTTG GGGGCGTCCG TGCGCTGCAG
  541 CGTCTCCTTC CCGTTCTCCA GGTATCTGCG GAGCCACTCC ACGCACGTGC CCTCCAGGTA
  601 GGCTCTCAAC TGCTCCGCCA CATGGGCCGC CTCCCACTTG TGCTTGGTGG TCTGAGCTGC
  661 CATGTCCGCC GCGGTCCAAG AGCGCAAGTC CTCTTTCAGG CGATGTAATC CTTGCCGTCG
  721 TAAGCGTACT GGTGGTACCC GCGAGGAAGC GCCAGTCCGA CCCCACGTCG CAGCCAACCA
  781 TCCTCTGGAC GGTGTGAAGA ACCGCCTCGC TCTGTGTAGT AGCCGCGCAG GTTCCCAGGT
  841 CCACTCGGTG AGTCTGTGAA TGGCTCACTT TCGTGTCTCC CGTCCAATAC TCGACCTCCT
  901 TGTCTTATCA AGGGCCGGGT CAATCCTCTG ATCCGGGGGG TCCTGTCACC GACAGACTGG
  961 TGTGTCTCCA CGTATCCATT GCATGAACGG TTCCCGGCGG CCGGACGAGT GGAAAATCCT
 1021 TTGATTAGAA CTGCCAGCCT GTCAGCCAGC TCGAGATCAG CAAGTTCGCT AGAGCCACTC
1081 GCTG
//
Primer: SV40pro_F_V2 RDB02868_A2K5c.seq
>02868_02868_A2K5_SV40pro_F_V2_E05_14.ab1
    1 AACAGCTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGTATA TCCATTTTCG
   61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
  121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
  181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
  241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
  301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
  361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
  421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
  481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
  541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
  601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
  661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
  721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
  781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC
  841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT
  901 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC
  961 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CCGGGACGCC CGGCTGGGAT
 1021 GATCCTCCAG CGCGGGGGAA TCTCATGCTG GAGTTCTTCG CCCACCCCAA CTGTTATGCA
 1081 GCTTATATGG ATACCGATTA AAGCCAATTA GCCATCACAA ACTTTCACAA ATAAAGCATT
1141 TTTTTTTT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl