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Human HLA-A*02:07 cDNA

Human HLA-A*02:07 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02868
Resource name Human HLA-A*02:07 cDNA
Alternative name HLA-A*0207
Clone info. Human HLA-A*02:07 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02868 Human HLA-A*02:07 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02868z.seq
Electronic file Map RDB02868.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15772_A8Aqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB15772_A8Aqa.seq check
>02868_15772_A8Aq_CMV-Forward_C01_07_ABI24.ab1
    1 CTTTACACTC GCCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGATT CTCCCCAGAC
  181 GCCGAGGATG GCCGTCATGG CGCCCCGAAC CCTCGTCCTG CTACTCTCGG GGGCTCTGGC
  241 CCTGACCCAG ACCTGGGCGG GCTCTCACTC CATGAGGTAT TTCTTCACAT CCGTGTCCCG
  301 GCCCGGCCGC GGGGAGCCCC GCTTCATCGC AGTGGGCTAC GTGGACGACA CGCAGTTCGT
  361 GCGGTTCGAC AGCGACGCCG CGAGCCAGAG GATGGAGCCG CGGGCGCCGT GGATAGAGCA
  421 GGAGGGTCCG GAGTATTGGG ACGGGGAGAC ACGGAAAGTG AAGGCCCACT CACAGACTCA
  481 CCGAGTGGAC CTGGGGACCC TGCGCGGCTA CTACAACCAG AGCGAGGCCG GTTCTCACAC
  541 CGTCCAGAGG ATGTGTGGCT GCGACGTGGG GTCGGACTGG CGCTTCCTCC GCGGGTACCA
  601 CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAA GAGGACCTGC GCTCTTGGAC
  661 CGCGGCGGAC ATGGCAGCTC AGACCACCAA GCACAAGTGG GAGGCGGCCC ATGTGGCGGA
  721 GCAGTTGAGA GCCTACCTGG AGGGCACGTG CGTGGAGTGG CTCCGCAGAT ACCTGGAGAA
  781 CGGGAAGGAG ACGCTGCAGC GCACGGACGC CCCCCAAAAC GCATATGACT CACCACGCTG
  841 TCTCTGACCA TGAAGCCACC CTGAGGTGCT GGGCCCTGAG CTTCTACCCT GCGGAGATCA
  901 CACTGACCTG GCAGCGGGAT GGGGAGGACC AGACCCAGGA CACGGAGCTC GTGGAGACCA
  961 GGCCCTGCAG GGGATGCAAC TTTCCAGAGT GGGGCGGCTG GTGTTGGTGC GTTTCTGGAC
1021 AGGAAGCCAG AGAATACACC TTGCATGCTG CAGCATGAAG ATTGGCTCAA GCTCCTCA
//
Primer: BGH_rev2
Region: bGH p(A), insert 3'
Sequence file: RDB15772_A8Aqb.seq check
>02868_15772_A8Aq_BGH_rev2_D01_10_ABI24.ab1
    1 GACCCGGGGT AAGGAAGCAC GGGGGAGGGG CAAACAACAG ATGGCTGGCA ACTAGAAGGC
   61 ACAGTCGAGG CTGATCAGCG GGTTTAAACG GGCCCTCTAG ACTCGAGCGG CCGCACAAGG
  121 CAGCTGTCTC ACACTTTACA AGCTGTGAGA GACACATCAG AGCCCTGGGC ACTGTCACTG
  181 CTTGCAGCCT GAGAGTAGCT CCCTCCTTTT CTATCTGAGC TCTTCCTCCT CCACATCACA
  241 GCAGCGACCA CAGCTCCAGT GATCACAGCT CCAAAGAGAA CCAGGCCAGC AATGATGCCC
  301 ACGATGGGGA TGGTGGGCTG GGAAGACGGC TCCCATCTCA GGGTGAGGGG CTTGGGCAAA
  361 CCCTCATGCT GCACATGGCA GGTGTATCTC TGCTCCTGTC CAGAAGGCAC CACCACAGCC
  421 GCCCACTTCT GGAAGGTTCC ATCCCCTGCA GGCCTGGTCT CCACGAGCTC CGTGTCCTGG
  481 GTCTGGTCCT CCCCATCCCG CTGCCAGGTC AGTGTGATCT CCGCAGGGTA GAAGCTCAGG
  541 GCCCAGCACC TCAGGGTGGC TTCATGGTCA GAGACAGCGT GGTGAGTCAT ATGCGTTTTG
  601 GGGGCGTCCG TGCGCTGCAG CGTCTCCTTC CCGTTCTCCA GGTATCTGCG GAGCCACTCC
  661 ACGCACGTGC CCTCCAGGTA GGCTCTCAAC TGCTCCGCCA CATGGGCCGC CTCCCACTTG
  721 TGCTTGGTGG TCTGAGCTGC CATGTCCGCC GCGGTCCAAG AGCGCAGGTC CTCTTTCAGG
  781 GCGATGTAAT CCTTGCCGTC GTAGGCGTAC TGGTGGTACC CGCGGAGGAA GCGCCAGTCC
  841 GACCCCACGT CGCAGCCCAC ACATCCTCTG GGACGGTGTG AGAACCGGCC TCGCTCTGGT
  901 TTGTAGTAGC CGCGCAGGGT CCCCAGGTCC ACTCGGGTGA GTCTGTGAGT GGGCCCTTCA
  961 CTTTCCCGTG TCTCCCCCGT CCCAATACTC CCGGACCCTC CTTGCTCTAT CCACGGGCGC
 1021 CCGCGGCTTC CATCCTCTGG CTCGCGGCGG TTCGGCTGTC GGAACCGCAC GAACTGGCGT
1081 GTCGGTCCAC GTAGCCTCAC TTGCGATGGA AAGGCCGGGG G
//
Primer: SV40pro_F_V2
Region: NeoR
Sequence file: RDB15772_A8Aqc.seq check
>02868_15772_B0FnF_SV40pro_F_V2_B11_05_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNGCTTTT GNNNNNNNNT CCCGGGNNCT TGTATATCCN
   61 TTTTCGGATC TGNNNNNNNN NCAGGNTGAG GATCGTTTCG CATGATTGAA CAAGATGGAT
  121 TGCACGCAGG TTCTCCGGCC GCTTGGGTGG AGAGGCTATT CGGCTATGAC TGGGCACAAC
  181 AGACAATCGG CTGCTCTGAT GCCGCCGTGT TCCGGCTGTC AGCGCAGGGG CGCCCGGTTC
  241 TTTTTGTCAA GACCGACCTG TCCGGTGCCC TGAATGAACT GCAGGACGAG GCAGCGCGGC
  301 TATCGTGGCT GGCCACGACG GGCGTTCCTT GCGCAGCTGT GCTCGACGTT GTCACTGAAG
  361 CGGGAAGGGA CTGGCTGCTA TTGGGCGAAG TGCCGGGGCA GGATCTCCTG TCATCTCACC
  421 TTGCTCCTGC CGAGAAAGTA TCCATCATGG CTGATGCAAT GCGGCGGCTG CATACGCTTG
  481 ATCCGGCTAC CTGCCCATTC GACCACCAAG CGAAACATCG CATCGAGCGA GCACGTACTC
  541 GGATGGAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG GGGCTCGCGC
  601 CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT CTCGTCGTGA
  661 CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT TCTGGATTCA
  721 TCGACTGTGG CCGGCTGGGT GTGGCGGANC GCTATCAGGA CATAGCGTTG GCTACCCGTG
  781 ATATTGCTGA AGAGCTTGGC GGCGAATGNG CTGANCGCTT CCTCGNGCTT TACGGNATCG
  841 CCGCTCCCGA NTCNCANCGC ATCGCCTTCT ATCGNNTTCT TGNCNANTNC TNCTGANCGG
  901 GNNNCNGGGG GNNCNAAATG ANCGANNNAN NCNNNNNCNN NNCCNNNNAT CNCNANANTT
961 NCNANNNCNN CCNNCNNCNN NNNNNNNAAN NNNNNGGNNN TNNNNNNNNN N
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.06.25

GNP_filter3_RDBDEP_html_200316.pl