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Human HLA-A*02:06 cDNA

Human HLA-A*02:06 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02867
Resource name Human HLA-A*02:06 cDNA
Alternative name HLA-A*0206
Clone info. Human HLA-A*02:06 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02867 Human HLA-A*02:06 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02867z.seq
Electronic file Map RDB02867.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13694_A5Jkp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB13694_A5Jka.seq check
>02867_13694_A5Jk_CMV-Forward_A07_01.ab1
    1 AGTTCACTCC GTCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
   61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
  121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGATT CTCCCCAGAC
  181 GCCGAGGATG GCCGTCATGG CGCCCCGAAC CCTCGTCCTG CTACTCTCGG GGGCTCTGGC
  241 CCTGACCCAG ACCTGGGCGG GCTCTCACTC CATGAGGTAT TTCTACACCT CCGTGTCCCG
  301 GCCCGGCCGC GGGGAGCCCC GCTTCATCGC AGTGGGCTAC GTGGACGACA CGCAGTTCGT
  361 GCGGTTCGAC AGCGACGCCG CGAGCCAGAG GATGGAGCCG CGGGCGCCGT GGATAGAGCA
  421 GGAGGGTCCG GAGTATTGGG ACGGGGAGAC ACGGAAAGTG AAGGCCCACT CACAGACTCA
  481 CCGAGTGGAC CTGGGGACCC TGCGCGGCTA CTACAACCAG AGCGAGGCCG GTTCTCACAC
  541 CGTCCAGAGG ATGTATGGCT GCGACGTGGG GTCGGACTGG CGCTTCCTCC GCGGGTACCA
  601 CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAA GAGGACCTGC GCTCTTGGAC
  661 CGCGGCGGAC ATGGCAGCTC AGACCACCAA GCACAAGTGG GAGGCGGCCC ATGTGGCGGA
  721 GCAGTTGAGA GCCTACCTGG AGGGCACGTG CGTGGAGTGG CTCCGCAGAT ACCTGGAGAA
  781 CGGGAAGGAG ACGCTGCAGC GCACGGACGC CCCCAAAACG CATATGACTC ACCACGCTGT
  841 CTCTGACCAT GAAGCCACCC TGAGGTGCTG GGCCCTGAGC TTCTACCCTG CGGAGATCAC
  901 ACTGACCTGG CAGCGGGATG GGGGAGGACC AGACCCAGGA CACGGAGCTC GTGGAGACCA
  961 GGCCTGCACG GGATGGAAAC CTTCCAGAAG TGGGCGGCTG TGGGTGGGTG CCCTTCTGGA
 1021 CAGGAGCAGA GATACACCCT GCCCATGTGC AGCATGAGGT TTGCCCAAAG CCCCTCACCC
1081 TGAGATGGGA AGTCGGTCCC TCCCAGCCCA CCATCCCCTC AATTCGTTGG GGC
//
Primer: BGH_rev
Region: insert 3'
Sequence file: RDB13694_A5Jkb.seq check
>02867_13694_A5Jk_BGHrev_B07_04.ab1
    1 GATTTCGGGG TCTCTAGACT CGAGCGGCCG CACAAGGCAG CTGTCTCACA CTTTACAAGC
   61 CGTGAGAGAC ACATCAGAGC CCTGGGCACT GTCACTGCTT GCAGCCTGAG AGTAGCTCCC
  121 TCCTTTTCTA TCTGAGCTCT TCCTCCTCCA CATCACAGCA GCGACCACAG CTCCAGTGAT
  181 CACAGCTCCA AAGAGAACCA GGCCAGCAAT GATGCCCACG ATGGGGATGG TGGGCTGGGA
  241 AGACGGCTCC CATCTCAGGG TGAGGGGCTT GGGCAAACCC TCATGCTGCA CATGGCAGGT
  301 GTATCTCTGC TCCTGTCCAG AAGGCACCAC CACAGCCGCC CACTTCTGGA AGGTTCCATC
  361 CCCTGCAGGC CTGGTCTCCA CGAGCTCCGT GTCCTGGGTC TGGTCCTCCC CATCCCGCTG
  421 CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCTCAGGGCC CAGCACCTCA GGGTGGCTTC
  481 ATGGTCAGAG ACAGCGTGGT GAGTCATATG CGTTTTGGGG GCGTCCGTGC GCTGCAGCGT
  541 CTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CCACTCCACG CACGTGCCCT CCAGGTAGGC
  601 TCTCAACTGC TCCGCCACAT GGGCCGCCTC CCACTTGTGC TTGGTGGTCT GAGCTGCCAT
  661 GTCCGCCGCG GTCCAAGAGC GCAGGTCCTC TTTCAGGGCG ATGTAATCCT TGCCGTCGTA
  721 GGCGTACTGG TGGTACCCGC GGAGGAAGCG CCAGTCCGAC CCCACGTCGC AGCCATACAT
  781 CCTCTGGACG GTGTGAGAAC CGGCCTCGCT CTGGTTGTAG TAGCCGCGCA GGGTCCCCAG
  841 GTCCACTCGG TGAGTCTGTG AGTGGGCCTT CACTTTCCGT GTCTCCCCGT CCCAATACTC
  901 CGGACCCTCC TGCTCTATCC ACGGCGCCCG CGGCTCCATC CTCTGGCTCG CGGCGTCGCT
  961 GTCGAACCGC ACGAACTGCG TGTCGTCCAC GTAGCCCACT GCGATGAAGC GGGGCTCCCC
 1021 GCGGGCCGGG CCGGGACACG GGAGGTGTAG AAAATACCCT CATGGTAGTG AGAGCCCGCC
1081 CAGGTCTGGG TCAAGACAGA GCTCCCGAGA GTAGCAAGGA CGAGGGGTTT CCGAG
//
Primer: SV40pro_F_V2
Region: NeoR
Sequence file: RDB13694_A5Jkc.seq check
>02867_13694_A5Jk_SV40pro_F_V2_C07_07.ab1
    1 GTTAACGTTT TTTGGGAGCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGT ATATCCATTT
   61 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
  121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
  181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
  241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT
  301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
  361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
  421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
  481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
  541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG
  601 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC
  661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
  721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA
  781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG
  841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC
  901 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC
  961 CACCGCCGCC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTTCCGGGG ACGCCGGGCT
 1021 GGGATGATCC TTCCAGCGCG GGGATCTCAT GCTGGAGGTT CTTTCGCCAA CCCCAACTTG
1081 TTTATTGCAG CTTATATTGC TTACCAATTA AGCCATAGGC ATTCACAAAT TTTCCACCA
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.06.25

GNP_filter3_RDBDEP_html_200316.pl