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Human HLA-A*02:06 cDNA

Human HLA-A*02:06 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02867
Resource name Human HLA-A*02:06 cDNA
Alternative name HLA-A*0206
Clone info. Human HLA-A*02:06 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human major histocompatibility complex, class I, A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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          RDB02867 Human HLA-A*02:06 cDNA DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02867z.seq
          Remarks, protocol and/or map (gif) RDB02867.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13694_A5Jkp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Sequence file: RDB13694_A5Jka.seq check
          >02867_13694_A5Jk_CMV-Forward_A07_01.ab1
              1 AGTTCACTCC GTCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA
             61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA
            121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGATT CTCCCCAGAC
            181 GCCGAGGATG GCCGTCATGG CGCCCCGAAC CCTCGTCCTG CTACTCTCGG GGGCTCTGGC
            241 CCTGACCCAG ACCTGGGCGG GCTCTCACTC CATGAGGTAT TTCTACACCT CCGTGTCCCG
            301 GCCCGGCCGC GGGGAGCCCC GCTTCATCGC AGTGGGCTAC GTGGACGACA CGCAGTTCGT
            361 GCGGTTCGAC AGCGACGCCG CGAGCCAGAG GATGGAGCCG CGGGCGCCGT GGATAGAGCA
            421 GGAGGGTCCG GAGTATTGGG ACGGGGAGAC ACGGAAAGTG AAGGCCCACT CACAGACTCA
            481 CCGAGTGGAC CTGGGGACCC TGCGCGGCTA CTACAACCAG AGCGAGGCCG GTTCTCACAC
            541 CGTCCAGAGG ATGTATGGCT GCGACGTGGG GTCGGACTGG CGCTTCCTCC GCGGGTACCA
            601 CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAA GAGGACCTGC GCTCTTGGAC
            661 CGCGGCGGAC ATGGCAGCTC AGACCACCAA GCACAAGTGG GAGGCGGCCC ATGTGGCGGA
            721 GCAGTTGAGA GCCTACCTGG AGGGCACGTG CGTGGAGTGG CTCCGCAGAT ACCTGGAGAA
            781 CGGGAAGGAG ACGCTGCAGC GCACGGACGC CCCCAAAACG CATATGACTC ACCACGCTGT
            841 CTCTGACCAT GAAGCCACCC TGAGGTGCTG GGCCCTGAGC TTCTACCCTG CGGAGATCAC
            901 ACTGACCTGG CAGCGGGATG GGGGAGGACC AGACCCAGGA CACGGAGCTC GTGGAGACCA
            961 GGCCTGCACG GGATGGAAAC CTTCCAGAAG TGGGCGGCTG TGGGTGGGTG CCCTTCTGGA
           1021 CAGGAGCAGA GATACACCCT GCCCATGTGC AGCATGAGGT TTGCCCAAAG CCCCTCACCC
          1081 TGAGATGGGA AGTCGGTCCC TCCCAGCCCA CCATCCCCTC AATTCGTTGG GGC
          //
          Primer: BGH_rev
          Sequence file: RDB13694_A5Jkb.seq check
          >02867_13694_A5Jk_BGHrev_B07_04.ab1
              1 GATTTCGGGG TCTCTAGACT CGAGCGGCCG CACAAGGCAG CTGTCTCACA CTTTACAAGC
             61 CGTGAGAGAC ACATCAGAGC CCTGGGCACT GTCACTGCTT GCAGCCTGAG AGTAGCTCCC
            121 TCCTTTTCTA TCTGAGCTCT TCCTCCTCCA CATCACAGCA GCGACCACAG CTCCAGTGAT
            181 CACAGCTCCA AAGAGAACCA GGCCAGCAAT GATGCCCACG ATGGGGATGG TGGGCTGGGA
            241 AGACGGCTCC CATCTCAGGG TGAGGGGCTT GGGCAAACCC TCATGCTGCA CATGGCAGGT
            301 GTATCTCTGC TCCTGTCCAG AAGGCACCAC CACAGCCGCC CACTTCTGGA AGGTTCCATC
            361 CCCTGCAGGC CTGGTCTCCA CGAGCTCCGT GTCCTGGGTC TGGTCCTCCC CATCCCGCTG
            421 CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCTCAGGGCC CAGCACCTCA GGGTGGCTTC
            481 ATGGTCAGAG ACAGCGTGGT GAGTCATATG CGTTTTGGGG GCGTCCGTGC GCTGCAGCGT
            541 CTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CCACTCCACG CACGTGCCCT CCAGGTAGGC
            601 TCTCAACTGC TCCGCCACAT GGGCCGCCTC CCACTTGTGC TTGGTGGTCT GAGCTGCCAT
            661 GTCCGCCGCG GTCCAAGAGC GCAGGTCCTC TTTCAGGGCG ATGTAATCCT TGCCGTCGTA
            721 GGCGTACTGG TGGTACCCGC GGAGGAAGCG CCAGTCCGAC CCCACGTCGC AGCCATACAT
            781 CCTCTGGACG GTGTGAGAAC CGGCCTCGCT CTGGTTGTAG TAGCCGCGCA GGGTCCCCAG
            841 GTCCACTCGG TGAGTCTGTG AGTGGGCCTT CACTTTCCGT GTCTCCCCGT CCCAATACTC
            901 CGGACCCTCC TGCTCTATCC ACGGCGCCCG CGGCTCCATC CTCTGGCTCG CGGCGTCGCT
            961 GTCGAACCGC ACGAACTGCG TGTCGTCCAC GTAGCCCACT GCGATGAAGC GGGGCTCCCC
           1021 GCGGGCCGGG CCGGGACACG GGAGGTGTAG AAAATACCCT CATGGTAGTG AGAGCCCGCC
          1081 CAGGTCTGGG TCAAGACAGA GCTCCCGAGA GTAGCAAGGA CGAGGGGTTT CCGAG
          //
          Primer: SV40pro_F_V2
          Sequence file: RDB13694_A5Jkc.seq check
          >02867_13694_A5Jk_SV40pro_F_V2_C07_07.ab1
              1 GTTAACGTTT TTTGGGAGCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGT ATATCCATTT
             61 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
            121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
            181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
            241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT
            301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
            361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
            421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
            481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
            541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG
            601 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC
            661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
            721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA
            781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG
            841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC
            901 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC
            961 CACCGCCGCC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTTCCGGGG ACGCCGGGCT
           1021 GGGATGATCC TTCCAGCGCG GGGATCTCAT GCTGGAGGTT CTTTCGCCAA CCCCAACTTG
          1081 TTTATTGCAG CTTATATTGC TTACCAATTA AGCCATAGGC ATTCACAAAT TTTCCACCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl