RDB02867 - Human HLA-A*02:06 cDNA

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Human HLA-A*02:06 cDNA

Human HLA-A*02:06 cDNA from human EBV-transformed B cell line cDNA


Catalog number	RDB02867
Resource name	Human HLA-A*02:06 cDNA
Alternative name	HLA-A*0206
Clone info.	Human HLA-A*02:06 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone	pcDNA3.1(+) (Plasmid)
Size of vector backbone	5.4 kb
Selectable markers	Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name	Human major histocompatibility complex, class I, A cDNA
Depositor	Akatsuka, Yoshiki \|
Other clones in our bank	human HLA-A (NCBI Gene 3105) \|

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).

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Terms and conditions set forth by the DEPOSITOR	In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
Ordering	Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word]

提供条件	利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
提供依頼	手続きの詳細は、「提供申込みについて」をご覧ください。提供依頼書 [Word] 提供同意書 [Word]

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB02867	Human HLA-A*02:06 cDNA	DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file	Sequence RDB02867z.seq from Depositor
Electronic file	Map RDB02867.gif from Depositor

Featured content	HLA Clones (en) (English text)
Featured content	HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet	RDB13694_A5Jkp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward	RDB13694_A5Jka.seq
>02867_13694_A5Jk_CMV-Forward_A07_01.ab1 1 AGTTCACTCC GTCCATTGAC GCAATGGGCG GTAGGCGTGT ACGGTGGGAG GTCTATATAA 61 GCAGAGCTCT CTGGCTAACT AGAGAACCCA CTGCTTACTG GCTTATCGAA ATTAATACGA 121 CTCACTATAG GGAGACCCAA GCTGGCTAGC GTTTAAACTT AAGCTTGATT CTCCCCAGAC 181 GCCGAGGATG GCCGTCATGG CGCCCCGAAC CCTCGTCCTG CTACTCTCGG GGGCTCTGGC 241 CCTGACCCAG ACCTGGGCGG GCTCTCACTC CATGAGGTAT TTCTACACCT CCGTGTCCCG 301 GCCCGGCCGC GGGGAGCCCC GCTTCATCGC AGTGGGCTAC GTGGACGACA CGCAGTTCGT 361 GCGGTTCGAC AGCGACGCCG CGAGCCAGAG GATGGAGCCG CGGGCGCCGT GGATAGAGCA 421 GGAGGGTCCG GAGTATTGGG ACGGGGAGAC ACGGAAAGTG AAGGCCCACT CACAGACTCA 481 CCGAGTGGAC CTGGGGACCC TGCGCGGCTA CTACAACCAG AGCGAGGCCG GTTCTCACAC 541 CGTCCAGAGG ATGTATGGCT GCGACGTGGG GTCGGACTGG CGCTTCCTCC GCGGGTACCA 601 CCAGTACGCC TACGACGGCA AGGATTACAT CGCCCTGAAA GAGGACCTGC GCTCTTGGAC 661 CGCGGCGGAC ATGGCAGCTC AGACCACCAA GCACAAGTGG GAGGCGGCCC ATGTGGCGGA 721 GCAGTTGAGA GCCTACCTGG AGGGCACGTG CGTGGAGTGG CTCCGCAGAT ACCTGGAGAA 781 CGGGAAGGAG ACGCTGCAGC GCACGGACGC CCCCAAAACG CATATGACTC ACCACGCTGT 841 CTCTGACCAT GAAGCCACCC TGAGGTGCTG GGCCCTGAGC TTCTACCCTG CGGAGATCAC 901 ACTGACCTGG CAGCGGGATG GGGGAGGACC AGACCCAGGA CACGGAGCTC GTGGAGACCA 961 GGCCTGCACG GGATGGAAAC CTTCCAGAAG TGGGCGGCTG TGGGTGGGTG CCCTTCTGGA 1021 CAGGAGCAGA GATACACCCT GCCCATGTGC AGCATGAGGT TTGCCCAAAG CCCCTCACCC 1081 TGAGATGGGA AGTCGGTCCC TCCCAGCCCA CCATCCCCTC AATTCGTTGG GGC //
Primer: BGH_rev	RDB13694_A5Jkb.seq
>02867_13694_A5Jk_BGHrev_B07_04.ab1 1 GATTTCGGGG TCTCTAGACT CGAGCGGCCG CACAAGGCAG CTGTCTCACA CTTTACAAGC 61 CGTGAGAGAC ACATCAGAGC CCTGGGCACT GTCACTGCTT GCAGCCTGAG AGTAGCTCCC 121 TCCTTTTCTA TCTGAGCTCT TCCTCCTCCA CATCACAGCA GCGACCACAG CTCCAGTGAT 181 CACAGCTCCA AAGAGAACCA GGCCAGCAAT GATGCCCACG ATGGGGATGG TGGGCTGGGA 241 AGACGGCTCC CATCTCAGGG TGAGGGGCTT GGGCAAACCC TCATGCTGCA CATGGCAGGT 301 GTATCTCTGC TCCTGTCCAG AAGGCACCAC CACAGCCGCC CACTTCTGGA AGGTTCCATC 361 CCCTGCAGGC CTGGTCTCCA CGAGCTCCGT GTCCTGGGTC TGGTCCTCCC CATCCCGCTG 421 CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCTCAGGGCC CAGCACCTCA GGGTGGCTTC 481 ATGGTCAGAG ACAGCGTGGT GAGTCATATG CGTTTTGGGG GCGTCCGTGC GCTGCAGCGT 541 CTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CCACTCCACG CACGTGCCCT CCAGGTAGGC 601 TCTCAACTGC TCCGCCACAT GGGCCGCCTC CCACTTGTGC TTGGTGGTCT GAGCTGCCAT 661 GTCCGCCGCG GTCCAAGAGC GCAGGTCCTC TTTCAGGGCG ATGTAATCCT TGCCGTCGTA 721 GGCGTACTGG TGGTACCCGC GGAGGAAGCG CCAGTCCGAC CCCACGTCGC AGCCATACAT 781 CCTCTGGACG GTGTGAGAAC CGGCCTCGCT CTGGTTGTAG TAGCCGCGCA GGGTCCCCAG 841 GTCCACTCGG TGAGTCTGTG AGTGGGCCTT CACTTTCCGT GTCTCCCCGT CCCAATACTC 901 CGGACCCTCC TGCTCTATCC ACGGCGCCCG CGGCTCCATC CTCTGGCTCG CGGCGTCGCT 961 GTCGAACCGC ACGAACTGCG TGTCGTCCAC GTAGCCCACT GCGATGAAGC GGGGCTCCCC 1021 GCGGGCCGGG CCGGGACACG GGAGGTGTAG AAAATACCCT CATGGTAGTG AGAGCCCGCC 1081 CAGGTCTGGG TCAAGACAGA GCTCCCGAGA GTAGCAAGGA CGAGGGGTTT CCGAG //
Primer: SV40pro_F_V2	RDB13694_A5Jkc.seq
>02867_13694_A5Jk_SV40pro_F_V2_C07_07.ab1 1 GTTAACGTTT TTTGGGAGCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGT ATATCCATTT 61 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC 121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA 181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT 241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT 301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG 361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG 421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC 481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA 541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG 601 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC 661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG 721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA 781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG 841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC 901 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC 961 CACCGCCGCC CTTCTATGAA AGGTTGGGCT TCGGAATCGT TTTTCCGGGG ACGCCGGGCT 1021 GGGATGATCC TTCCAGCGCG GGGATCTCAT GCTGGAGGTT CTTTCGCCAA CCCCAACTTG 1081 TTTATTGCAG CTTATATTGC TTACCAATTA AGCCATAGGC ATTCACAAAT TTTCCACCA //

Please visit Sequencing and PCR primers for primer information.

References

original	Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl