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Human HLA-A*02:01:01 cDNA

Human HLA-A*02:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02866
Resource name Human HLA-A*02:01:01 cDNA
Alternative name HLA-A*02011
Clone info. Human HLA-A*02:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02866 Human HLA-A*02:01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02866z.seq from Depositor
Electronic file Map RDB02866.gif from Depositor

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13051_A5A5.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13051_A5A5a.seq
>02866_13051_A5A5_CMV-Forward_A02_02.ab1
    1 GTAGGGGTGG GTCACCTGTC ACTCCGCCCA TTGACGCAAT GGGCGGTAGG CGTGTACGGT
   61 GGGAGGTCTA TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA
  121 TCGAAATTAA TACGACTCAC TATAGGGAGA CCCAAGCTGG CTAGCGTTTA AACTTAAGCT
  181 TGATTCTCCC CAGACGCCGA GGATGGCCGT CATGGCGCCC CGAACCCTCG TCCTGCTACT
  241 CTCGGGGGCT CTGGCCCTGA CCCAGACCTG GGCGGGCTCT CACTCCATGA GGTATTTCTT
  301 CACATCCGTG TCCCGGCCCG GCCGCGGGGA GCCCCGCTTC ATCGCAGTGG GCTACGTGGA
  361 CGACACGCAG TTCGTGCGGT TCGACAGCGA CGCCGCGAGC CAGAGGATGG AGCCGCGGGC
  421 GCCGTGGATA GAGCAGGAGG GTCCGGAGTA TTGGGACGGG GAGACACGGA AAGTGAAGGC
  481 CCACTCACAG ACTCACCGAG TGGACCTGGG GACCCTGCGC GGCTACTACA ACCAGAGCGA
  541 GGCCGGTTCT CACACCGTCC AGAGGATGTA TGGCTGCGAC GTGGGGTCGG ACTGGCGCTT
  601 CCTCCGCGGG TACCACCAGT ACGCCTACGA CGGCAAGGAT TACATCGCCC TGAAAGAGGA
  661 CCTGCGCTCT TGGACCGCGG CGGACATGGC AGCTCAGACC ACCAAGCACA AGTGGGAGGC
  721 GGCCCATGTG GCGGAGCAGT TGAGAGCCTA CCTGGAGGGC ACGTGCGTGG AGTGGCTCCG
  781 CAGATACCTG GAGAACGGGA AGGAGACGCT GCAGCGCACG GACGCCCCCA AAACGCATAT
  841 GACTCACCAC GCTGTCTCTG ACCATGAAGC CACCCTGAGG TGCTGGGCCC TGAGCTTCTA
  901 CCCTGCGGAG ATCACACTGA CCTGGCAGCG GGATGGGGAG GACCAGACCC AGGACACGGA
  961 GCTCGTGGAG ACCAGGCCTG CAGGGGATGG AACCTTCCAG AAGTGGGCGG CTGTGGTGGT
 1021 GCCTTCTGGG ACAGGAGCAG AGATACACCT GCCATGTGCA GCATGAGGGT TTGCCCCAAG
 1081 CCCCTTCACC TGAGATTGGA GGCCCGTCTT CCCCAGCCCA CCCATTCCCC CAATCCGATG
1141 GG
//
Primer: BGH_rev RDB13051_A5A5b.seq
>02866_13051_A5A5_BGHrev_B02_05.ab1
    1 GATGTCGGGG TCCTCTAGAC TCGAGCGCCG CACAAGGCAG CTGTCTCACA CTTTACAAGC
   61 TGTGAGAGAC ACATCAGAGC CCTGGGCACT GTCACTGCTT GCAGCCTGAG AGTAGCTCCC
  121 TCCTTTTCTA TCTGAGCTCT TCCTCCTCCA CATCACAGCA GCGACCACAG CTCCAGTGAT
  181 CACAGCTCCA AAGAGAACCA GGCCAGCAAT GATGCCCACG ATGGGGATGG TGGGCTGGGA
  241 AGACGGCTCC CATCTCAGGG TGAGGGGCTT GGGCAAACCC TCATGCTGCA CATGGCAGGT
  301 GTATCTCTGC TCCTGTCCAG AAGGCACCAC CACAGCCGCC CACTTCTGGA AGGTTCCATC
  361 CCCTGCAGGC CTGGTCTCCA CGAGCTCCGT GTCCTGGGTC TGGTCCTCCC CATCCCGCTG
  421 CCAGGTCAGT GTGATCTCCG CAGGGTAGAA GCTCAGGGCC CAGCACCTCA GGGTGGCTTC
  481 ATGGTCAGAG ACAGCGTGGT GAGTCATATG CGTTTTGGGG GCGTCCGTGC GCTGCAGCGT
  541 CTCCTTCCCG TTCTCCAGGT ATCTGCGGAG CCACTCCACG CACGTGCCCT CCAGGTAGGC
  601 TCTCAACTGC TCCGCCACAT GGGCCGCCTC CCACTTGTGC TTGGTGGTCT GAGCTGCCAT
  661 GTCCGCCGCG GTCCAAGAGC GCAGGTCCTC TTTCAGGGCG ATGTAATCCT TGCCGTCGTA
  721 GGCGTACTGG TGGTACCCGC GGAGGAAGCG CCAGTCCGAC CCCACGTCGC AGCCATACAT
  781 CCTCTGGACG GTGTGAGAAC CGGCCTCGCT CTGGTTGTAG TAGCCGCGCA GGGTCCCCAG
  841 GTCCACTCGG TGAGTCTGTG AGTGGGCCTT CACTTTCCGT GTCTCCCCGT CCCAATACTC
  901 CGGACCCTCC TGCTCTATCC ACGGCGCCCG CGGCTCCATC CTCTGGCTCG CGGCGTCGCT
  961 GTCGAACCGC ACGAACTGCG TGTCGTCCAC GTAGCCCACT GCGATGAAGC GGGGCTCCCC
 1021 GCGGCCCGGG CCGGGACACG GGATGTGAAG AAATACCCTC AATGCAGGTG AGAGCCCGCC
 1081 CAGGATCTGG GTTCAAGGAT CAGAGCCCCC GGAGAGTAGG CAAGAACGAA GGGGATTCCG
1141 GGGGGCGG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2018.12.11

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