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Human HLA-A*02:01:01 cDNA

Human HLA-A*02:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02866
Resource name Human HLA-A*02:01:01 cDNA
Alternative name HLA-A*02011
Clone info. Human HLA-A*02:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
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External Database
Human major histocompatibility complex, class I, A

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
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          RDB02866 Human HLA-A*02:01:01 cDNA DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Sequence RDB02866z.seq
          Remarks, protocol and/or map (gif) RDB02866.gif

          Original reference

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13051_A6Bcp1-4.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV_Forward
          Region: CMV pro,insert 5'
          Sequence file: RDB13051_A6Bcd.seq check
          >02866_13051_B2FdS_CMV_Forward_D05_11_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNTTG NNNCNNTGGG CGNTANGCGT GTACNGTGGG ANGTCTATAT
             61 NAGCAGAGCT CTCTGGCTAA CTAGAGAACC CACTGCTTAC TGGCTTATCG AAATTAATAC
            121 GACTCACTAT AGGGAGACCC AAGCTGGCTA GCGTTTAAAC TTAAGCTTGA TTCTCCCCAG
            181 ACGCCGAGGA TGGCCGTCAT GGCGCCCCGA ACCCTCGTCC TGCTACTCTC GGGGGCTCTG
            241 GCCCTGACCC AGACCTGGGC GGGCTCTCAC TCCATGAGGT ATTTCTTCAC ATCCGTGTCC
            301 CGGCCCGGCC GCGGGGAGCC CCGCTTCATC GCAGTGGGCT ACGTGGACGA CACGCAGTTC
            361 GTGCGGTTCG ACAGCGACGC CGCGAGCCAG AGGATGGAGC CGCGGGCGCC GTGGATAGAG
            421 CAGGAGGGTC CGGAGTATTG GGACGGGGAG ACACGGAAAG TGAAGGCCCA CTCACAGACT
            481 CACCGAGTGG ACCTGGGGAC CCTGCGCGGC TACTACAACC AGAGCGAGGC CGGTTCTCAC
            541 ACCGTCCAGA GGATGTATGG CTGCGACGTG GGGTCGGACT GGCGCTTCCT CCGCGGGTAC
            601 CACCAGTACG CCTACGACGG CAAGGATTAC ATCGCCCTGA AAGAGGACCT GCGCTCTTGG
            661 ACCGCGGCGG ACATGGCAGC TCAGACCACC AAGCACAAGT GGGANGCGGC CCATGTGGCG
            721 GANCAGTTGA GAGCCTACCT GGAGGGCACG TGCGTGGAGT GGCTCCGCAG ATACCTGGAG
            781 AACGGGAANG AGACGCTGCA GCGCACGGAC GCCNCCNAAA CGCATATGAN TCACCACGCT
            841 GTCTCTGANC ATGAAGCCAC CNNGANGTGC TGGGCCCNNA NCTTCNACCC TGCNGANATC
            901 ACACNNNNCN NNCAGCNGGN TGGGGANGAN CAGANCCAGG NNANNNNNCT CGNGGANNNN
            961 ANNCCNGNNN GGNNNGGNNN CNTTNCNNAN NTGGNNNNNT NNNGNNNGNN NNCNTNNNNG
          1021 NNNNNNNNNN
          //
          Primer: bGH_rev3
          Region: bGHpA,insert 3'
          Sequence file: RDB13051_A6Bce.seq check
          >02866_13051_B2FdS_bGH_rev3_E05_14_ABI24.ab1
              1 NNNNNNNNNN NNNNGGNGGN NGGGNANNNA CAGATGGCTG GCAACTAGAA GGCACAGTCG
             61 AGGCTGATCA GCGGGTTTAA ACGGGCCCTC TAGACTCGAG CGCCGCACAA GGCAGCTGTC
            121 TCACACTTTA CAAGCTGTGA GAGACACATC AGAGCCCTGG GCACTGTCAC TGCTTGCAGC
            181 CTGAGAGTAG CTCCCTCCTT TTCTATCTGA GCTCTTCCTC CTCCACATCA CAGCAGCGAC
            241 CACAGCTCCA GTGATCACAG CTCCAAAGAG AACCAGGCCA GCAATGATGC CCACGATGGG
            301 GATGGTGGGC TGGGAAGACG GCTCCCATCT CAGGGTGAGG GGCTTGGGCA AACCCTCATG
            361 CTGCACATGG CAGGTGTATC TCTGCTCCTG TCCAGAAGGC ACCACCACAG CCGCCCACTT
            421 CTGGAAGGTT CCATCCCCTG CAGGCCTGGT CTCCACGAGC TCCGTGTCCT GGGTCTGGTC
            481 CTCCCCATCC CGCTGCCAGG TCAGTGTGAT CTCCGCAGGG TAGAAGCTCA GGGCCCAGCA
            541 CCTCAGGGTG GCTTCATGGT CAGAGACAGC GTGGTGAGTC ATATGCGTTT TGGGGGCGTC
            601 CGTGCGCTGC AGCGTCTCCT TCCCGTTCTC CAGGTATCTG CGGAGCCACT CCACGCACGT
            661 GCCCTCCAGG TAGGCTCTCA ACTGCTCCGC CACATGGGCC GCCTCCCACT TGTGCTTGGT
            721 GGTCTGANCT GCCATGTCCG CCGCGGTCCA AGAGCGCANG TCCTCTTTCA NGGCGATGTA
            781 ATCCTTGCCG TCGTANGCGT ACTGGTGGTA CCCGCGGANG AAGCGCCNGT CCGANNNCAC
            841 GTCGCANCCN TACATCCTCT GGACNGTGTG ANAANCGGNC TCGCTCTGGT TGTAGTANNC
            901 NCGCNNGNTC CCCANGNNCA NTCNGNNANT NTGTNNNTGG GNCTTNNCTT TNCGNGNNTC
            961 CCCGNNCCNN NNNNNCNNNA NCNNNNNNNN NTATNNNNNN NGCCNNNGGN NNCNTNNNNN
          1021 NNNNN
          //
          Primer: SV40pro_F
          Region: NeoR
          Sequence file: RDB13051_A6Bcf.seq check
          >02866_13051_B0FnF_SV40pro_F_V2_A11_02_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT GNNNNANGCT CCCGGGNNCT TGNNNNNNCA
             61 TTTTCGGATC TGNNNNNNNA NNCNGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA
            121 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA
            181 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT
            241 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG
            301 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA
            361 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC
            421 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT
            481 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT
            541 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG
            601 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG
            661 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC
            721 ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCAGG ACATAGCGTT GGCTACCCGT
            781 GATATTGCTG AAGAGCTTGN CGGCGAATGG GCTGANCGCT TCCTCGNGCT TTACGGTATC
            841 GCCNCTCCCG ATTCNCAGCG CATCGCNTTC TATCGCNTNN TTGANGANTN NNNCTGANCN
            901 GGANTNNGGG GGTTCNAAAN GNNNGANNNA NCNNNNNCNN NCNNGNNNNT CNNNAGNNTT
          961 CGATTNNNNN NNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

          2022.06.23

          GNP_filter3_RDBDEP_html_220520.pl