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Human HLA-A*02:01:01 cDNA

Human HLA-A*02:01:01 cDNA from human EBV-transformed B cell line cDNA

Catalog number RDB02866
Resource name Human HLA-A*02:01:01 cDNA
Alternative name HLA-A*02011
Clone info. Human HLA-A*02:01:01 cDNA from human EBV-transformed B cell line cDNA.
Vector backbone pcDNA3.1(+) (Plasmid)
Size of vector backbone 5.4 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human major histocompatibility complex, class I, A cDNA
Depositor|Developer Akatsuka, Yoshiki |
 
Other clones in our bank human HLA-A (NCBI Gene 3105) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Akatsuka et al., Tissue Antigens 59, 502-511 (2002).).
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RDB02866 Human HLA-A*02:01:01 cDNA DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Electronic file Sequence RDB02866z.seq
Electronic file Map RDB02866.gif

Original reference

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13051_A6Bcp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward
Region: CMV pro, insert 5'
Sequence file: RDB13051_A6Bca.seq check
>02866_13051_A6Bc_CMV-Forward_G07_19.ab1
    1 CTTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TGGCTAGCGT TTAAACTTAA GCTTGATTCT CCCCAGACGC
  181 CGAGGATGGC CGTCATGGCG CCCCGAACCC TCGTCCTGCT ACTCTCGGGG GCTCTGGCCC
  241 TGACCCAGAC CTGGGCGGGC TCTCACTCCA TGAGGTATTT CTTCACATCC GTGTCCCGGC
  301 CCGGCCGCGG GGAGCCCCGC TTCATCGCAG TGGGCTACGT GGACGACACG CAGTTCGTGC
  361 GGTTCGACAG CGACGCCGCG AGCCAGAGGA TGGAGCCGCG GGCGCCGTGG ATAGAGCAGG
  421 AGGGTCCGGA GTATTGGGAC GGGGAGACAC GGAAAGTGAA GGCCCACTCA CAGACTCACC
  481 GAGTGGACCT GGGGACCCTG CGCGGCTACT ACAACCAGAG CGAGGCCGGT TCTCACACCG
  541 TCCAGAGGAT GTATGGCTGC GACGTGGGGT CGGACTGGCG CTTCCTCCGC GGGTACCACC
  601 AGTACGCCTA CGACGGCAAG GATTACATCG CCCTGAAAGA GGACCTGCGC TCTTGGACCG
  661 CGGCGGACAT GGCAGCTCAG ACCACCAAGC ACAAGTGGGA GGCGGCCCAT GTGGCGGAGC
  721 AGTTGAGAGC CTACCTGGAG GGCACGTGCG TGGAGTGGCT CCGCAGATAC CTGGAGAACG
  781 GGAAGGAGAC GCTGCAGCGC ACGGACGCCC CCAAAACGCA TATGACTCAC CACGCTGTCT
  841 CTGACCATGA AGCCACCCTG AGGTGCTGGG CCCTGAGCTT CTACCCTGCG GAGATCACAC
  901 TGACCTGGCA GCGGGATGGG GGAGGGACCA GACCCAGGAC ACGGAGCTCG TGGAGACCAG
  961 GCCTGCAGGG GATGGGACCT TTCCAGAGTG GCGCTGTGGT TGAGCGTCTG ACAGAAGCAG
 1021 AGAATACACC TGGCATGTGC AGCAATGAGC TTGCTAGGCC CGTTCAGCCT GAGAATGGGG
1081 A
//
Primer: BGH_rev
Region: insert 3'
Sequence file: RDB13051_A6Bcb.seq check
>02866_13051_A6Bc_BGHrev_H07_22.ab1
    1 GTTCGGCTCT AGACTCGAGC GCCGCACAAG GCAGCTGTCT CACACTTTAC AAGCTGTGAG
   61 AGACACATCA GAGCCCTGGG CACTGTCACT GCTTGCAGCC TGAGAGTAGC TCCCTCCTTT
  121 TCTATCTGAG CTCTTCCTCC TCCACATCAC AGCAGCGACC ACAGCTCCAG TGATCACAGC
  181 TCCAAAGAGA ACCAGGCCAG CAATGATGCC CACGATGGGG ATGGTGGGCT GGGAAGACGG
  241 CTCCCATCTC AGGGTGAGGG GCTTGGGCAA ACCCTCATGC TGCACATGGC AGGTGTATCT
  301 CTGCTCCTGT CCAGAAGGCA CCACCACAGC CGCCCACTTC TGGAAGGTTC CATCCCCTGC
  361 AGGCCTGGTC TCCACGAGCT CCGTGTCCTG GGTCTGGTCC TCCCCATCCC GCTGCCAGGT
  421 CAGTGTGATC TCCGCAGGGT AGAAGCTCAG GGCCCAGCAC CTCAGGGTGG CTTCATGGTC
  481 AGAGACAGCG TGGTGAGTCA TATGCGTTTT GGGGGCGTCC GTGCGCTGCA GCGTCTCCTT
  541 CCCGTTCTCC AGGTATCTGC GGAGCCACTC CACGCACGTG CCCTCCAGGT AGGCTCTCAA
  601 CTGCTCCGCC ACATGGGCCG CCTCCCACTT GTGCTTGGTG GTCTGAGCTG CCATGTCCGC
  661 CGCGGTCCAA GAGCGCAGGT CCTCTTTCAG GGCGATGTAA TCCTTGCCGT CGTAGGCGTA
  721 CTGGTGGTAC CCGCGGAGGA AGCGCCAGTC CGACCCCACG TCGCAGCCAT ACATCCTCTG
  781 GACGGTGTGA GAACCGGCCT CGCTCTGGTT GTAGTAGCCG CGCAGGGTCC CCAGGTCCAC
  841 TCGGTGAGTC TGTGAGTGGG CCTTCACTTT CCGTGTCTCC CCGTCCCAAT ACTCCGGGAC
  901 CCTCCTGCTC TATCCACGGC GCCGCGGCTC CATCTCTGGC TCGCGGCGTC GCTGTCGACC
  961 GCACGACTGC GTGTCGTTCA CGTTAGGCCC ACTGCGAATG AGCGGGGGCT TCCCGCGTCG
 1021 GGTAGGACAC GGATGTGCAG ATAGTCATGC ATGAAGCTGA CGAGTCTAGT CAGATCAGAG
1081 TC
//
Primer: SV40pro_F_V2
Region: NeoR
Sequence file: RDB13051_A6Bcc.seq check
>02866_13051_B0FnF_SV40pro_F_V2_A11_02_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNTT GNNNNANGCT CCCGGGNNCT TGNNNNNNCA
   61 TTTTCGGATC TGNNNNNNNA NNCNGGATGA GGATCGTTTC GCATGATTGA ACAAGATGGA
  121 TTGCACGCAG GTTCTCCGGC CGCTTGGGTG GAGAGGCTAT TCGGCTATGA CTGGGCACAA
  181 CAGACAATCG GCTGCTCTGA TGCCGCCGTG TTCCGGCTGT CAGCGCAGGG GCGCCCGGTT
  241 CTTTTTGTCA AGACCGACCT GTCCGGTGCC CTGAATGAAC TGCAGGACGA GGCAGCGCGG
  301 CTATCGTGGC TGGCCACGAC GGGCGTTCCT TGCGCAGCTG TGCTCGACGT TGTCACTGAA
  361 GCGGGAAGGG ACTGGCTGCT ATTGGGCGAA GTGCCGGGGC AGGATCTCCT GTCATCTCAC
  421 CTTGCTCCTG CCGAGAAAGT ATCCATCATG GCTGATGCAA TGCGGCGGCT GCATACGCTT
  481 GATCCGGCTA CCTGCCCATT CGACCACCAA GCGAAACATC GCATCGAGCG AGCACGTACT
  541 CGGATGGAAG CCGGTCTTGT CGATCAGGAT GATCTGGACG AAGAGCATCA GGGGCTCGCG
  601 CCAGCCGAAC TGTTCGCCAG GCTCAAGGCG CGCATGCCCG ACGGCGAGGA TCTCGTCGTG
  661 ACCCATGGCG ATGCCTGCTT GCCGAATATC ATGGTGGAAA ATGGCCGCTT TTCTGGATTC
  721 ATCGACTGTG GCCGGCTGGG TGTGGCGGAN CGCTATCAGG ACATAGCGTT GGCTACCCGT
  781 GATATTGCTG AAGAGCTTGN CGGCGAATGG GCTGANCGCT TCCTCGNGCT TTACGGTATC
  841 GCCNCTCCCG ATTCNCAGCG CATCGCNTTC TATCGCNTNN TTGANGANTN NNNCTGANCN
  901 GGANTNNGGG GGTTCNAAAN GNNNGANNNA NCNNNNNCNN NCNNGNNNNT CNNNAGNNTT
961 CGATTNNNNN NNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Akatsuka, Y., Efficient cloning and expression of HLA class I cDNA in human B-lymphoblastoid cell lines. Tissue Antigens, 59, 502-511 (2002). PMID 12445320.

2020.06.25

GNP_filter3_RDBDEP_html_200316.pl