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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEAK-Claudin-5 (#RDB02846)

Human claudin-5 cDNA

Clone info. Expression vector of human claudin-5 cDNA. Expression was confirmed by the depositor with Western blotting.
Comment pEAK-Claudin-5 ( RDB02846 ) contains 335-1699nt of NM_003277.3 ( length : 1720bp, CDS : 216..1127 ), the current version of RefSeq entry for the Claudin-5 mRNA.
Vector backbone pEAK8 (Plasmid)
Size of vector backbone 7 kp
Selectable markers Amp^r
Gene/insert name Human Claudin-5 cDNA
Depositor|Developer Hroshi, Sato |
 

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biol. Chem., 276, 28204-28211 (2001)).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem., 276, 28204-28211 (2001))。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02846 pEAK-Claudin-5 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEAK-Claudin-5 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02846).

Reference section:

Miyamori, H., Takino, T., Kobayashi, Y., Tokai, H., Itoh, Y., Seiki, M., Sato, H., Claudin promotes activation of pro-matrix metalloproteinase-2 mediated by membrane-type matrix metalloproteinases. J. Biol. Chem., 276, 28204-28211 (2001). PMID 11382769. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13713_A5Krp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: BGH_rev
Sequence file: RDB13713_A5Kra.seq check
>02846_13713_A5Kr_pAxEF_F1_A09_03.ab1
    1 CTTTTTTTCT TTCAATTTCA GGTGTCGTGA AAGCTTGAAT TCGGCGCGCC AGATATCACA
   61 CGTGCCAAGG GGCTGGCTCA CAGACCCGCG GGGCAAACGG ACTGGGGCCA AGAGCCGGGA
  121 GCGCGGGCGC AAAGGCACCA GGGCCCGCCC AGGGCGCCGC GCAGCACGGC CTTGGGGGTT
  181 CTGCGGGCCT TCGGGTGCGC GTCTCGCCTC TAGCCATGGG GTCCGCAGCG TTGGAGATCC
  241 TGGGCCTGGT GCTGTGCCTG GTGGGCTGGG GGGGTCTGAT CCTGGCGTGC GGGCTGCCCA
  301 TGTGGCAGGT GACCGCCTTC CTGGACCACA ACATCGTGAC GGCGCAGACC ACCTGGAAGG
  361 GGCTGTGGAT GTCGTGCGTG GTGCAGAGCA CCGGGCACAT GCAGTGCAAA GTGTACGACT
  421 CGGTGCTGGC TCTGAGCACC GAGGTGCAGG CGGCGCGGGC GCTCACCGTG AGCGCCGTGC
  481 TGCTGGCGTT CGTTGCGCTC TTCGTGACCC TGGCGGGCGC GCAGTGCACC ACCTGCGTGG
  541 CCCCGGGCCC GGCCAAGGCG CGTGTGGCCC TCACGGGAGG CGTGCTCTAC CTGTTTTGCG
  601 GGCTGCTGGC GCTCGTGCCA CTCTGCTGGT TCGCCAACAT TGTCGTCCGC GAGTTTTACG
  661 ACCCGTCTGT GCCCGTGTCG CAGAAGTACG AGCTGGGCGC AGCGCTGTAC ATCGGCTGGG
  721 CGGCCACCGC GCTGCTCATG GTAGGCGGCT GCCTCTTGTG CTGCGGCGCC TGGGTCTGCA
  781 CCGGCCGTCC CGACCTCAGC TTCCCCGTGA AGTACTCAGC GCCGCGGCGG CCCACGGCCA
  841 CCGGCGACTA CGACAAGAAG AACTACGTCT GAGGGCGCTG GGCACGGCCG GGCCCCTCCT
  901 GCCAGCCACG CCTGCGAGGC GTTGGATAAG CCTGGGGGAG CCCCGCATGG ACCGCGGCTT
  961 CCGCCGGGTA GCGCGGCGCG CAGGCTCCTC GGAACGTCCG GCTCTGCGCC CCGACGCGGC
 1021 TCCTGGATCC CGCTCCCTGC CCTGCGCCCG CAGCTGACTT TCTCTGCCAC TAGGCCCGGT
 1081 CCTGCCGTTT AACAGACGGA ATTGAACTTC CGTTTTCAGT GCGCCTGGAC GCTGATATCC
1141 CAAG
//
Primer: 3'LTR,PRE
Sequence file: RDB13713_A5Krb.seq check
>02846_13713_A5Kr_pAxEF_R2_B09_06.ab1
    1 CGGGCAAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
   61 GCTGCAATAA ACAAGTTAAC TTGGGCCCCT TGCTCCATAC CACCCGCCTC CACCCCATAA
  121 TATTATACAA GGACACCTAG TCAAACAAAA TGATGCAACT TAATTTTATT AGGACAAGGC
  181 TGGTGGGCAC TGGAGTAGCA CCTTCCACGA CCAGGAGAGG CACTGGGGAG GGGTCACAGG
  241 GATGCCACCC GGGCAGACCT GAGGAAGAGA TGGAGGTGGA CGTGTCAGCA CCCGGCTGGG
  301 GCCTGTCCCT GGATGCAGGC TACTCTAGGG CACCTGTCCC GCCTTGAGCT GGAGGGCGAG
  361 GCCTGGGCTG GCTTACCTGC GGCCGCCTGC AGGCCAGGTG CGGAGAAAGA GGTAATGTTT
  421 TTTTTTTTTT TTTTTTTTTT TTTGAGAGTT CAAACCATTT ACTAAGCAGA TTCTTAGCCT
  481 TCCCACTCCC GCCCTCTCTC AAGCTCCGGT GCCCACAAGC CTTGCCTGGG GAGATGCTGG
  541 AGTGAGACCG GGAGGTCCAG GCCAAGTCAC TGGTCCCTGG GCTCGGGCCC TGCCGATGGA
  601 GTAAAGACCA GCTGTACACA TCTTCCGGTG GGGGCCCTGG GCTCTGCATC CGCCCCTCCG
  661 AAGTCAGCAG GAGCCTCTGG GAAGTAAGGC AGCAGCCAAG ACCCCCAGCG TCTTGGAGGG
  721 GAAGCGAAAT CCTCAGTCTG ACACCCGCTC TGCCTATGGA AACAGCGCCG CGCACAGAAA
  781 AGGAAACTTC ATTCCGTCTG TTAAGGGCAG GGCCGGGCTA GTGGCAGGAG AAGGTCAGCT
  841 GCGGGCGCAG GCAGGAGCGG ATCCAGGAGC CGCGTCGGGG CGCAGAGCCG GACGTTCCGA
  901 GGAGCCTGCG CGCCGCGCTA CCCGGCGGAA GCCGCGGTCC ATGCGGGGCT CCCCAGGCTT
  961 ATCCAACGCC TCGCAGCGTG GCTGGCAGGA GGGGCCCGGG CCGTGCCCAG CGCCCTCAGA
 1021 CGTAGTTCTT CTTGTCGTAG TCGCCGGTGG TCGTGGGGCC GCCCGCGGCG CTGAGTTACC
 1081 TCACGGGGAA GCTGAGTTCC GGAACGGTCG ATGCAGACCC AAGCGCCGCA GCACAAGGAA
1141 GGCCAGGCCG ACCTACA
//
Primer: pAxCALNL_F1
Sequence file: RDB13713_A5Krc.seq check
>02846_13713_A5Kr_pAxCALNL_F1_C09_09.ab1
    1 TTAATCTATC TTATCATGTC TGGATCCGCT TCAGGCACCG GGCTTGCGGG TCATGCACCA
   61 GGTGCGCGGT CCTTCGGGCA CCTCGACGTC GGCGGTGACG GTGAAGCCGA GCCGCTCGTA
  121 GAAGGGGAGG TTGCGGGGCG CGGAGGTCTC CAGGAAGGCG GGCACCCCGG CGCGCTCGGC
  181 CGCCTCCACT CCGGGGAGCA CGACGGCGCT GCCCAGACCC TTGCCCTGGT GGTCGGGCGA
  241 GACGCCGACG GTGGCCAGGA ACCACGCGGG CTCCTTGGGC CGGTGCGGCG CCAGGAGGCC
  301 TTCCATCTGT TGCTGCGCGG CCAGCCTGGA ACCGCTCAAC TCGGCCATGC GCGGGCCGAT
  361 CTCGGCGAAC ACCGCCCCCG CTTCGACGCT CTCCGGCGTG GTCCAGACCG CCACCGCGGC
  421 GCCGTCGTCC GCGACCCACA CCTTGCCGAT GTCGAGCCCG ACGCGCGTGA GGAAGAGTTC
  481 TTGCAGCTCG GTGACCCGCT CGATGTGGCG GTCCGGGTCG ACGGTGTGGC GCGTGGCGGG
  541 GTAGTCGGCG AACGCGGCGG CGAGGGTGCG TACGGCCCGG GGGACGTCGT CGCGGGTGGC
  601 GAGGCGCACC GTGGGCTTGT ACTCGGTCAT GGTGGCCTGC AGAGTCGCTC TGTGTTCGAG
  661 GCCACACGCG TCACCTTAAT ATGCGAAGTG GACCTGGGAC CGCGCCGCCC CGACTGCATC
  721 TGCGTGTTTT CGCCAATGAC AAGACGCTGG GCGGGGTTTG TGTCATCATA GAACTAAAGA
  781 CATGCAAATA TATTTCTTCC GGGGACACCG CCAGCAAACG CGAGCAACGG GCCACGGGGA
  841 TGAAGCAGCT GCGCCACTCC CTGAAGATCC ATCGTCTCCT AACAAGTTAC ATCACTCCTG
  901 CCCTTCCTCA CCCTCATCTC CATCACCTCC TTCATCTCCG TCATCTCCGT CATCACCCTC
  961 CGCGGCAGCC CCTTCCACCA TATGTGGAAA CCAGGGAGGC AAATCTACTC CATCGTCAAA
 1021 GCTGCACACA GTCACCCTGA TATTGCAGTA GGAGCGGGGC TTTGTTCATA ACACGTCCCT
 1081 TAAATCGCAT CCCTCCAAAT CTTCAGCCAA ATAATATGAG TTTGGTAAAA AAGACCCAAT
1141 TGAAAATATA ACCAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miyamori, H., Claudin promotes activation of pro-matrix metalloproteinase-2 mediated by membrane-type matrix metalloproteinases. J. Biol. Chem., 276, 28204-28211 (2001). PMID 11382769. [link to RRC of NBRP]