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pEAK-Claudin-5

Human claudin-5 cDNA

Catalog number RDB02846
Resource name pEAK-Claudin-5
Clone info. Expression vector of human claudin-5 cDNA. Expression was confirmed by the depositor with Western blotting.
pEAK-Claudin-5 ( RDB02846 ) contains 335-1699nt of NM_003277.3 ( length : 1720bp, CDS : 216..1127 ), the current version of RefSeq entry for the Claudin-5 mRNA.
Vector backbone pEAK8 (Plasmid)
Size of vector backbone 7 kp
Selectable markers Amp^r
Gene/insert name Human Claudin-5 cDNA
Depositor Sato, Hiroshi |
 
Other clones in our bank human CLDN5 (NCBI Gene 7122) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biol. Chem., 276, 28204-28211 (2001)).
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RDB02846 pEAK-Claudin-5 DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Featured content

References

original Miyamori, H., Claudin promotes activation of pro-matrix metalloproteinase-2 mediated by membrane-type matrix metalloproteinases. J. Biol. Chem., 276, 28204-28211 (2001). PMID 11382769.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13713_A5Krp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxEF_F1 RDB13713_A5Kra.seq
>02846_13713_A5Kr_pAxEF_F1_A09_03.ab1
    1 CTTTTTTTCT TTCAATTTCA GGTGTCGTGA AAGCTTGAAT TCGGCGCGCC AGATATCACA
   61 CGTGCCAAGG GGCTGGCTCA CAGACCCGCG GGGCAAACGG ACTGGGGCCA AGAGCCGGGA
  121 GCGCGGGCGC AAAGGCACCA GGGCCCGCCC AGGGCGCCGC GCAGCACGGC CTTGGGGGTT
  181 CTGCGGGCCT TCGGGTGCGC GTCTCGCCTC TAGCCATGGG GTCCGCAGCG TTGGAGATCC
  241 TGGGCCTGGT GCTGTGCCTG GTGGGCTGGG GGGGTCTGAT CCTGGCGTGC GGGCTGCCCA
  301 TGTGGCAGGT GACCGCCTTC CTGGACCACA ACATCGTGAC GGCGCAGACC ACCTGGAAGG
  361 GGCTGTGGAT GTCGTGCGTG GTGCAGAGCA CCGGGCACAT GCAGTGCAAA GTGTACGACT
  421 CGGTGCTGGC TCTGAGCACC GAGGTGCAGG CGGCGCGGGC GCTCACCGTG AGCGCCGTGC
  481 TGCTGGCGTT CGTTGCGCTC TTCGTGACCC TGGCGGGCGC GCAGTGCACC ACCTGCGTGG
  541 CCCCGGGCCC GGCCAAGGCG CGTGTGGCCC TCACGGGAGG CGTGCTCTAC CTGTTTTGCG
  601 GGCTGCTGGC GCTCGTGCCA CTCTGCTGGT TCGCCAACAT TGTCGTCCGC GAGTTTTACG
  661 ACCCGTCTGT GCCCGTGTCG CAGAAGTACG AGCTGGGCGC AGCGCTGTAC ATCGGCTGGG
  721 CGGCCACCGC GCTGCTCATG GTAGGCGGCT GCCTCTTGTG CTGCGGCGCC TGGGTCTGCA
  781 CCGGCCGTCC CGACCTCAGC TTCCCCGTGA AGTACTCAGC GCCGCGGCGG CCCACGGCCA
  841 CCGGCGACTA CGACAAGAAG AACTACGTCT GAGGGCGCTG GGCACGGCCG GGCCCCTCCT
  901 GCCAGCCACG CCTGCGAGGC GTTGGATAAG CCTGGGGGAG CCCCGCATGG ACCGCGGCTT
  961 CCGCCGGGTA GCGCGGCGCG CAGGCTCCTC GGAACGTCCG GCTCTGCGCC CCGACGCGGC
 1021 TCCTGGATCC CGCTCCCTGC CCTGCGCCCG CAGCTGACTT TCTCTGCCAC TAGGCCCGGT
 1081 CCTGCCGTTT AACAGACGGA ATTGAACTTC CGTTTTCAGT GCGCCTGGAC GCTGATATCC
1141 CAAG
//
Primer: pAxEF_R2 RDB13713_A5Krb.seq
>02846_13713_A5Kr_pAxEF_R2_B09_06.ab1
    1 CGGGCAAAAT GCTTTATTTG TGAATTTGTG ATGCTATTGC TTTATTTGTA ACCATTATAA
   61 GCTGCAATAA ACAAGTTAAC TTGGGCCCCT TGCTCCATAC CACCCGCCTC CACCCCATAA
  121 TATTATACAA GGACACCTAG TCAAACAAAA TGATGCAACT TAATTTTATT AGGACAAGGC
  181 TGGTGGGCAC TGGAGTAGCA CCTTCCACGA CCAGGAGAGG CACTGGGGAG GGGTCACAGG
  241 GATGCCACCC GGGCAGACCT GAGGAAGAGA TGGAGGTGGA CGTGTCAGCA CCCGGCTGGG
  301 GCCTGTCCCT GGATGCAGGC TACTCTAGGG CACCTGTCCC GCCTTGAGCT GGAGGGCGAG
  361 GCCTGGGCTG GCTTACCTGC GGCCGCCTGC AGGCCAGGTG CGGAGAAAGA GGTAATGTTT
  421 TTTTTTTTTT TTTTTTTTTT TTTGAGAGTT CAAACCATTT ACTAAGCAGA TTCTTAGCCT
  481 TCCCACTCCC GCCCTCTCTC AAGCTCCGGT GCCCACAAGC CTTGCCTGGG GAGATGCTGG
  541 AGTGAGACCG GGAGGTCCAG GCCAAGTCAC TGGTCCCTGG GCTCGGGCCC TGCCGATGGA
  601 GTAAAGACCA GCTGTACACA TCTTCCGGTG GGGGCCCTGG GCTCTGCATC CGCCCCTCCG
  661 AAGTCAGCAG GAGCCTCTGG GAAGTAAGGC AGCAGCCAAG ACCCCCAGCG TCTTGGAGGG
  721 GAAGCGAAAT CCTCAGTCTG ACACCCGCTC TGCCTATGGA AACAGCGCCG CGCACAGAAA
  781 AGGAAACTTC ATTCCGTCTG TTAAGGGCAG GGCCGGGCTA GTGGCAGGAG AAGGTCAGCT
  841 GCGGGCGCAG GCAGGAGCGG ATCCAGGAGC CGCGTCGGGG CGCAGAGCCG GACGTTCCGA
  901 GGAGCCTGCG CGCCGCGCTA CCCGGCGGAA GCCGCGGTCC ATGCGGGGCT CCCCAGGCTT
  961 ATCCAACGCC TCGCAGCGTG GCTGGCAGGA GGGGCCCGGG CCGTGCCCAG CGCCCTCAGA
 1021 CGTAGTTCTT CTTGTCGTAG TCGCCGGTGG TCGTGGGGCC GCCCGCGGCG CTGAGTTACC
 1081 TCACGGGGAA GCTGAGTTCC GGAACGGTCG ATGCAGACCC AAGCGCCGCA GCACAAGGAA
1141 GGCCAGGCCG ACCTACA
//
Primer: pAxCALNL_F1 RDB13713_A5Krc.seq
>02846_13713_A5Kr_pAxCALNL_F1_C09_09.ab1
    1 TTAATCTATC TTATCATGTC TGGATCCGCT TCAGGCACCG GGCTTGCGGG TCATGCACCA
   61 GGTGCGCGGT CCTTCGGGCA CCTCGACGTC GGCGGTGACG GTGAAGCCGA GCCGCTCGTA
  121 GAAGGGGAGG TTGCGGGGCG CGGAGGTCTC CAGGAAGGCG GGCACCCCGG CGCGCTCGGC
  181 CGCCTCCACT CCGGGGAGCA CGACGGCGCT GCCCAGACCC TTGCCCTGGT GGTCGGGCGA
  241 GACGCCGACG GTGGCCAGGA ACCACGCGGG CTCCTTGGGC CGGTGCGGCG CCAGGAGGCC
  301 TTCCATCTGT TGCTGCGCGG CCAGCCTGGA ACCGCTCAAC TCGGCCATGC GCGGGCCGAT
  361 CTCGGCGAAC ACCGCCCCCG CTTCGACGCT CTCCGGCGTG GTCCAGACCG CCACCGCGGC
  421 GCCGTCGTCC GCGACCCACA CCTTGCCGAT GTCGAGCCCG ACGCGCGTGA GGAAGAGTTC
  481 TTGCAGCTCG GTGACCCGCT CGATGTGGCG GTCCGGGTCG ACGGTGTGGC GCGTGGCGGG
  541 GTAGTCGGCG AACGCGGCGG CGAGGGTGCG TACGGCCCGG GGGACGTCGT CGCGGGTGGC
  601 GAGGCGCACC GTGGGCTTGT ACTCGGTCAT GGTGGCCTGC AGAGTCGCTC TGTGTTCGAG
  661 GCCACACGCG TCACCTTAAT ATGCGAAGTG GACCTGGGAC CGCGCCGCCC CGACTGCATC
  721 TGCGTGTTTT CGCCAATGAC AAGACGCTGG GCGGGGTTTG TGTCATCATA GAACTAAAGA
  781 CATGCAAATA TATTTCTTCC GGGGACACCG CCAGCAAACG CGAGCAACGG GCCACGGGGA
  841 TGAAGCAGCT GCGCCACTCC CTGAAGATCC ATCGTCTCCT AACAAGTTAC ATCACTCCTG
  901 CCCTTCCTCA CCCTCATCTC CATCACCTCC TTCATCTCCG TCATCTCCGT CATCACCCTC
  961 CGCGGCAGCC CCTTCCACCA TATGTGGAAA CCAGGGAGGC AAATCTACTC CATCGTCAAA
 1021 GCTGCACACA GTCACCCTGA TATTGCAGTA GGAGCGGGGC TTTGTTCATA ACACGTCCCT
 1081 TAAATCGCAT CCCTCCAAAT CTTCAGCCAA ATAATATGAG TTTGGTAAAA AAGACCCAAT
1141 TGAAAATATA ACCAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Miyamori, H., Claudin promotes activation of pro-matrix metalloproteinase-2 mediated by membrane-type matrix metalloproteinases. J. Biol. Chem., 276, 28204-28211 (2001). PMID 11382769.

2018.12.10

GNP_filter3_RDBDEP_html_181012.pl