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pEF-Flag-mSTATY705F

Expression vector of mouse STAT3 dominant negative mutant.

Catalog number RDB02354
Resource name pEF-Flag-mSTATY705F
Clone info. Expression vector for dominant negative mouse STAT3. 312-2621nt of NM_213659.3 (cds 309-2621) is cloned. Wild type [pEF-Flag-mSTAT3 (wild), RDB02353] is available.
Known differences (at least): compared with NM_213659.3, G to T substitution at 2416 nt (Leu, synonym).
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone ca. 5.2 kp
Selectable markers Amp^r
Gene/insert name Mouse STAT3 cDNA
Depositor Akira, Shizuo |
 
Other clones in our bank mouse Stat3 (NCBI Gene 20848) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02354 pEF-Flag-mSTATY705F DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB02354.gif from Depositor

Featured content

Featured content Jak-STAT signaling pathway (English text)

References

original Minami, M., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14394_A6Kap1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB14394_A6Kaa.seq
>02354_14394_A6Ka_EF1a-C_F_C05_08_ABI24.ab1
    1 GTCCCTTGGG AATTTTGCCC TTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA
   61 CAGGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGGGTCGA
  121 CGGATTATGG ACTACAAGGA CGACGATGAC AAGGCTCAGT GGAACCAGCT GCAGCAGCTG
  181 GACACACGCT ACCTGGAGCA GCTGCACCAG CTGTACAGCG ACAGCTTCCC CATGGAGCTG
  241 CGGCAGTTCC TGGCACCTTG GATTGAGAGT CAAGACTGGG CATATGCAGC CAGCAAAGAG
  301 TCACATGCCA CGTTGGTGTT TCATAATCTC TTGGGTGAAA TTGACCAGCA ATATAGCCGA
  361 TTCCTGCAAG AGTCCAATGT CCTCTATCAG CACAACCTTC GAAGAATCAA GCAGTTTCTG
  421 CAGAGCAGGT ATCTTGAGAA GCCAATGGAA ATTGCCCGGA TCGTGGCCCG ATGCCTGTGG
  481 GAAGAGTCTC GCCTCCTCCA GACGGCAGCC ACGGCAGCCC AGCAAGGGGG CCAGGCCAAC
  541 CACCCAACAG CCGCCGTAGT GACAGAGAAG CAGCAGATGT TGGAGCAGCA TCTTCAGGAT
  601 GTCCGGAAGC GAGTGCAGGA TCTAGAACAG AAAATGAAGG TGGTGGAGAA CCTCCAGGAC
  661 GACTTTGATT TCAACTACAA AACCCTCAAG AGCCAAGGAG ACATGCAGGA TCTGAATGGA
  721 AACAACCAGT CTGTGACCAG ACAGAAGATG CAGCAGCTGG AACAGATGCT CACAGCCCTG
  781 GACCAGATGC GGAGAAGCAT TGTGAGTGAG CTGGCGGGGC TCTTGTCAGC AATGGAGTAC
  841 GTGCAGAAGA CACTGACTGA TGAAGAGCTG GCTGACTGGA AGAGGCGGCA GCAGATCGGC
  901 GTGCATCGGA GGCCCTCCCA ACATCTGCCT GGACCGTCTG GAAAACTGGA TAACTTCATT
  961 AGCAGAATCT CAACTTCAGA CCCGCCCAAC AATTTAAGAA ACTGGGAGGG AGCTGCAGCA
 1021 GAAAGTGTCC TAACAAGGGG CGACCCTATC GTTGCAGCAC CGGCCCCAAT GCTGGGAGAG
 1081 AGGGATCGTG GGAGCTGTCA GAACTTTAAT GAAGGAGTGC CTCGTGGTGA GCCGCCAGGC
1141 CCCTGCATGC CCCATGCCAC CCCGGGAACC CGGG
//
Primer: hGCSF-R RDB14394_A6Kab.seq
>02354_14394_A6Ka_hGCSF-R_D05_11_ABI24.ab1
    1 CTTGAAGGAA TGAATACTCT CAGGGTCCCA GGTGGGGACC CTCACTCTAG GGTCGACTCA
   61 CATGGGGGAG GTAGCACACT CCGAGGTCAG ATCCATGTCA AACGTGAGCG ACTCAAACTG
  121 CCCTCCTGCT GAGGGCTCAG CACCTTCACC GTTATTTCCA AACTGCATCA ATGAATCTAA
  181 AGTGCGGGGG GACATCGGCA GGTCAATGGT ATTGCTGCAG GTCGTTGGTG TCACACAGAT
  241 GAACTTGGTC TTAAGGAACG GGGCAGCACT ACCTGGGTCG GCTTCGGGGT GCTCCTGGCT
  301 CTCGGGCCTA CAGTACTTTC CAAATGCCTC CTCCTTGGGA ATGTCGGGGT AGAGGTAGAC
  361 AAGTGGAGAC ACCAGGATGT TGGTCGCATC CATGATCTTA TAGCCCATGA TGATTTCAGC
  421 AAATGACATG TTGTTCAGCT GCTGCTTGGT GTATGGCTCT ACAGACTGGA TCTGGGTCTT
  481 GCCACTGATG TCCTTTTCCA CCCAAGTGAA AGTGACCCCT CCTTCTTTGC TGCTCTCGCT
  541 GAAGCGCAGT AGGAAGGTGC CCGGGGGCTT TGTGCTTAGG ATGGCCCGCT CCCGCTCCTT
  601 GCTGATGAAA CCCATGATGT ACCCTTCATT CCAAAGGGCC AAGATATACT TTTTCACAAG
  661 GTCGATGATA TTGTCTAGCC AGACCCAGAA GGAGAAGCCC TTGCCAGCCA TGTTTTCTTT
  721 GCAGAATTTA GCCCATGTGA TCTGACACCC TGAGTAGTTC ACACCAGGCC CTAGGAGCTT
  781 CTCAGCCAGC GTTGTCAGCT GCTCGATGCT CAGCCCCCGC TTGGTGGTGG ACGAGAACTG
  841 CCAGCTGAGC ACCTCGGCCC ACTTGGTCCC AGGTTCCAAT TGGCGGCTTA GTGAAGAAGT
  901 TCACGTTCTT GGGGTTATTG GTCAGCATGT TATACCACAG GATTGATGCC CCAAGCATTT
  961 GGCATCTGAC AGATGTTGGA GATCACCACA ACTGGGCAAG GAGTGGGTCT CTAGGTCATC
 1021 TTTGAAGCCC TTGGTGGTAC ACCTCAGTCT CGGAAGGTGA TCAGGTGCAG CTCTCAGTCA
 1081 CGATTCAAGG AGGCATCACA ATTGCACGGC CTCCATTCCC ACATCTCTGC TCCCTTAGGG
1141 CTCAAGTGCC TTGAACTCTG GCAGACACAG GCGTCGTCCC GCG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Minami, M., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998.
user report Shin, S.Y., Transcriptional regulation of the interleukin-11 gene by oncogenic Ras. Carcinogenesis, 33 (12): 2467-2476 (2012). PMID 23027619.
user report Chiba, T., Stat3 inhibition in neural lineage cells. Horm. Mol. Biol. Clin. Investig. 10 (2): 255-263 (2012). PMID 25436682.
user report Nakamura H., Mouse model of human infertility: transient and local inhibition of endometrial STAT-3 activation results in implantation failure. FEBS Lett., 580, 2717-2722 (2006). PMID 16647058.

2019.03.04

GNP_filter3_RDBDEP_html_190131.pl