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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Flag-mSTATY705F (#RDB02354)

Expression vector of mouse STAT3 dominant negative mutant.

Clone info. Expression vector for dominant negative mouse STAT3. 312-2621nt of NM_213659.3 (cds 309-2621) is cloned. Wild type [pEF-Flag-mSTAT3 (wild), RDB02353] is available.
Comment Known differences (at least): compared with NM_213659.3, G to T substitution at 2416 nt (Leu, synonym).
Vector backbone pEF-BOS (RDB07939) (Plasmid)
Size of vector backbone ca. 5.2 kp
Selectable markers Amp^r
Gene/insert name Mouse STAT3 cDNA
Depositor|Developer Akira, Shizuo |
 
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Distribution information

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Terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
MTAに書く付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02354 pEF-Flag-mSTATY705F DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pEF-Flag-mSTATY705F was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02354).

Reference section:

Minami, M., Inoue, M., Wei, S., Takeda, K., Matsumoto, M., Kishimoto, T., Akira, S., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14394_A6Kap1-2.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F ()
Sequence file: RDB14394_A6Kaa.seq check
>02354_14394_A6Ka_EF1a-C_F_C05_08_ABI24.ab1
    1 GTCCCTTGGG AATTTTGCCC TTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA
   61 CAGGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGGGTCGA
  121 CGGATTATGG ACTACAAGGA CGACGATGAC AAGGCTCAGT GGAACCAGCT GCAGCAGCTG
  181 GACACACGCT ACCTGGAGCA GCTGCACCAG CTGTACAGCG ACAGCTTCCC CATGGAGCTG
  241 CGGCAGTTCC TGGCACCTTG GATTGAGAGT CAAGACTGGG CATATGCAGC CAGCAAAGAG
  301 TCACATGCCA CGTTGGTGTT TCATAATCTC TTGGGTGAAA TTGACCAGCA ATATAGCCGA
  361 TTCCTGCAAG AGTCCAATGT CCTCTATCAG CACAACCTTC GAAGAATCAA GCAGTTTCTG
  421 CAGAGCAGGT ATCTTGAGAA GCCAATGGAA ATTGCCCGGA TCGTGGCCCG ATGCCTGTGG
  481 GAAGAGTCTC GCCTCCTCCA GACGGCAGCC ACGGCAGCCC AGCAAGGGGG CCAGGCCAAC
  541 CACCCAACAG CCGCCGTAGT GACAGAGAAG CAGCAGATGT TGGAGCAGCA TCTTCAGGAT
  601 GTCCGGAAGC GAGTGCAGGA TCTAGAACAG AAAATGAAGG TGGTGGAGAA CCTCCAGGAC
  661 GACTTTGATT TCAACTACAA AACCCTCAAG AGCCAAGGAG ACATGCAGGA TCTGAATGGA
  721 AACAACCAGT CTGTGACCAG ACAGAAGATG CAGCAGCTGG AACAGATGCT CACAGCCCTG
  781 GACCAGATGC GGAGAAGCAT TGTGAGTGAG CTGGCGGGGC TCTTGTCAGC AATGGAGTAC
  841 GTGCAGAAGA CACTGACTGA TGAAGAGCTG GCTGACTGGA AGAGGCGGCA GCAGATCGGC
  901 GTGCATCGGA GGCCCTCCCA ACATCTGCCT GGACCGTCTG GAAAACTGGA TAACTTCATT
  961 AGCAGAATCT CAACTTCAGA CCCGCCCAAC AATTTAAGAA ACTGGGAGGG AGCTGCAGCA
 1021 GAAAGTGTCC TAACAAGGGG CGACCCTATC GTTGCAGCAC CGGCCCCAAT GCTGGGAGAG
 1081 AGGGATCGTG GGAGCTGTCA GAACTTTAAT GAAGGAGTGC CTCGTGGTGA GCCGCCAGGC
1141 CCCTGCATGC CCCATGCCAC CCCGGGAACC CGGG
//
Primer: hGCSF-R
Sequence file: RDB14394_A6Kab.seq check
>02354_14394_A6Ka_hGCSF-R_D05_11_ABI24.ab1
    1 CTTGAAGGAA TGAATACTCT CAGGGTCCCA GGTGGGGACC CTCACTCTAG GGTCGACTCA
   61 CATGGGGGAG GTAGCACACT CCGAGGTCAG ATCCATGTCA AACGTGAGCG ACTCAAACTG
  121 CCCTCCTGCT GAGGGCTCAG CACCTTCACC GTTATTTCCA AACTGCATCA ATGAATCTAA
  181 AGTGCGGGGG GACATCGGCA GGTCAATGGT ATTGCTGCAG GTCGTTGGTG TCACACAGAT
  241 GAACTTGGTC TTAAGGAACG GGGCAGCACT ACCTGGGTCG GCTTCGGGGT GCTCCTGGCT
  301 CTCGGGCCTA CAGTACTTTC CAAATGCCTC CTCCTTGGGA ATGTCGGGGT AGAGGTAGAC
  361 AAGTGGAGAC ACCAGGATGT TGGTCGCATC CATGATCTTA TAGCCCATGA TGATTTCAGC
  421 AAATGACATG TTGTTCAGCT GCTGCTTGGT GTATGGCTCT ACAGACTGGA TCTGGGTCTT
  481 GCCACTGATG TCCTTTTCCA CCCAAGTGAA AGTGACCCCT CCTTCTTTGC TGCTCTCGCT
  541 GAAGCGCAGT AGGAAGGTGC CCGGGGGCTT TGTGCTTAGG ATGGCCCGCT CCCGCTCCTT
  601 GCTGATGAAA CCCATGATGT ACCCTTCATT CCAAAGGGCC AAGATATACT TTTTCACAAG
  661 GTCGATGATA TTGTCTAGCC AGACCCAGAA GGAGAAGCCC TTGCCAGCCA TGTTTTCTTT
  721 GCAGAATTTA GCCCATGTGA TCTGACACCC TGAGTAGTTC ACACCAGGCC CTAGGAGCTT
  781 CTCAGCCAGC GTTGTCAGCT GCTCGATGCT CAGCCCCCGC TTGGTGGTGG ACGAGAACTG
  841 CCAGCTGAGC ACCTCGGCCC ACTTGGTCCC AGGTTCCAAT TGGCGGCTTA GTGAAGAAGT
  901 TCACGTTCTT GGGGTTATTG GTCAGCATGT TATACCACAG GATTGATGCC CCAAGCATTT
  961 GGCATCTGAC AGATGTTGGA GATCACCACA ACTGGGCAAG GAGTGGGTCT CTAGGTCATC
 1021 TTTGAAGCCC TTGGTGGTAC ACCTCAGTCT CGGAAGGTGA TCAGGTGCAG CTCTCAGTCA
 1081 CGATTCAAGG AGGCATCACA ATTGCACGGC CTCCATTCCC ACATCTCTGC TCCCTTAGGG
1141 CTCAAGTGCC TTGAACTCTG GCAGACACAG GCGTCGTCCC GCG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Minami, M., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998. [link to RRC of NBRP]
user_report Nakamura, H., An In Vivo Screening Model for Investigation of Pathophysiology of Human Implantation Failure. Biomolecules 13 (1): 79 (2022). PMID 36671464. [link to RRC of NBRP]
user_report Shin, S.Y., Transcriptional regulation of the interleukin-11 gene by oncogenic Ras. Carcinogenesis, 33 (12): 2467-2476 (2012). PMID 23027619. [link to RRC of NBRP]
user_report Chiba, T., Stat3 inhibition in neural lineage cells. Horm. Mol. Biol. Clin. Investig. 10 (2): 255-263 (2012). PMID 25436682. [link to RRC of NBRP]
user_report Nakamura H., Mouse model of human infertility: transient and local inhibition of endometrial STAT-3 activation results in implantation failure. FEBS Lett., 580, 2717-2722 (2006). PMID 16647058. [link to RRC of NBRP]