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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pEF-Flag-mSTATY705F

Expression vector of mouse STAT3 dominant negative mutant.

Catalog number RDB02354
Resource name pEF-Flag-mSTATY705F
Clone info. Expression vector for dominant negative mouse STAT3. 312-2621nt of NM_213659.3 (cds 309-2621) is cloned. Wild type [pEF-Flag-mSTAT3 (wild), RDB02353] is available.
Comment Known differences (at least): compared with NM_213659.3, G to T substitution at 2416 nt (Leu, synonym).
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone ca. 5.2 kp
Selectable markers Amp^r
Gene/insert name Mouse STAT3 cDNA
Depositor|Developer Akira, Shizuo |
Other clones in our bank
External Database
Mouse STAT3
          Reference sequence
            
           
          Remarks, protocol and/or map (gif) RDB02354.gif

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
          Additional terms and conditions:
          Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
          付加的提供条件：
          pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB02354 pEF-Flag-mSTATY705F DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pEF-Flag-mSTATY705F was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02354).

          Reference section:

          Minami, M., Inoue, M., Wei, S., Takeda, K., Matsumoto, M., Kishimoto, T., Akira, S., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998.

          Further references such as user reports and related articles (go to bottom)

          References and tips

          Featured content

          Featured content Jak-STAT signaling pathway (English text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14394_A6Kap1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F ()
          Sequence file: RDB14394_A6Kaa.seq check
          >02354_14394_A6Ka_EF1a-C_F_C05_08_ABI24.ab1
              1 GTCCCTTGGG AATTTTGCCC TTTTTTGAGT TTGGATCTTG GTTCATTCTC AAGCCTCAGA
             61 CAGGTGGTTC AAAGTTTTTT TCTTCCATTT CAGGTGTCGT GAGGAATTCT CTAGGGTCGA
            121 CGGATTATGG ACTACAAGGA CGACGATGAC AAGGCTCAGT GGAACCAGCT GCAGCAGCTG
            181 GACACACGCT ACCTGGAGCA GCTGCACCAG CTGTACAGCG ACAGCTTCCC CATGGAGCTG
            241 CGGCAGTTCC TGGCACCTTG GATTGAGAGT CAAGACTGGG CATATGCAGC CAGCAAAGAG
            301 TCACATGCCA CGTTGGTGTT TCATAATCTC TTGGGTGAAA TTGACCAGCA ATATAGCCGA
            361 TTCCTGCAAG AGTCCAATGT CCTCTATCAG CACAACCTTC GAAGAATCAA GCAGTTTCTG
            421 CAGAGCAGGT ATCTTGAGAA GCCAATGGAA ATTGCCCGGA TCGTGGCCCG ATGCCTGTGG
            481 GAAGAGTCTC GCCTCCTCCA GACGGCAGCC ACGGCAGCCC AGCAAGGGGG CCAGGCCAAC
            541 CACCCAACAG CCGCCGTAGT GACAGAGAAG CAGCAGATGT TGGAGCAGCA TCTTCAGGAT
            601 GTCCGGAAGC GAGTGCAGGA TCTAGAACAG AAAATGAAGG TGGTGGAGAA CCTCCAGGAC
            661 GACTTTGATT TCAACTACAA AACCCTCAAG AGCCAAGGAG ACATGCAGGA TCTGAATGGA
            721 AACAACCAGT CTGTGACCAG ACAGAAGATG CAGCAGCTGG AACAGATGCT CACAGCCCTG
            781 GACCAGATGC GGAGAAGCAT TGTGAGTGAG CTGGCGGGGC TCTTGTCAGC AATGGAGTAC
            841 GTGCAGAAGA CACTGACTGA TGAAGAGCTG GCTGACTGGA AGAGGCGGCA GCAGATCGGC
            901 GTGCATCGGA GGCCCTCCCA ACATCTGCCT GGACCGTCTG GAAAACTGGA TAACTTCATT
            961 AGCAGAATCT CAACTTCAGA CCCGCCCAAC AATTTAAGAA ACTGGGAGGG AGCTGCAGCA
           1021 GAAAGTGTCC TAACAAGGGG CGACCCTATC GTTGCAGCAC CGGCCCCAAT GCTGGGAGAG
           1081 AGGGATCGTG GGAGCTGTCA GAACTTTAAT GAAGGAGTGC CTCGTGGTGA GCCGCCAGGC
          1141 CCCTGCATGC CCCATGCCAC CCCGGGAACC CGGG
          //
          Primer: hGCSF-R
          Sequence file: RDB14394_A6Kab.seq check
          >02354_14394_A6Ka_hGCSF-R_D05_11_ABI24.ab1
              1 CTTGAAGGAA TGAATACTCT CAGGGTCCCA GGTGGGGACC CTCACTCTAG GGTCGACTCA
             61 CATGGGGGAG GTAGCACACT CCGAGGTCAG ATCCATGTCA AACGTGAGCG ACTCAAACTG
            121 CCCTCCTGCT GAGGGCTCAG CACCTTCACC GTTATTTCCA AACTGCATCA ATGAATCTAA
            181 AGTGCGGGGG GACATCGGCA GGTCAATGGT ATTGCTGCAG GTCGTTGGTG TCACACAGAT
            241 GAACTTGGTC TTAAGGAACG GGGCAGCACT ACCTGGGTCG GCTTCGGGGT GCTCCTGGCT
            301 CTCGGGCCTA CAGTACTTTC CAAATGCCTC CTCCTTGGGA ATGTCGGGGT AGAGGTAGAC
            361 AAGTGGAGAC ACCAGGATGT TGGTCGCATC CATGATCTTA TAGCCCATGA TGATTTCAGC
            421 AAATGACATG TTGTTCAGCT GCTGCTTGGT GTATGGCTCT ACAGACTGGA TCTGGGTCTT
            481 GCCACTGATG TCCTTTTCCA CCCAAGTGAA AGTGACCCCT CCTTCTTTGC TGCTCTCGCT
            541 GAAGCGCAGT AGGAAGGTGC CCGGGGGCTT TGTGCTTAGG ATGGCCCGCT CCCGCTCCTT
            601 GCTGATGAAA CCCATGATGT ACCCTTCATT CCAAAGGGCC AAGATATACT TTTTCACAAG
            661 GTCGATGATA TTGTCTAGCC AGACCCAGAA GGAGAAGCCC TTGCCAGCCA TGTTTTCTTT
            721 GCAGAATTTA GCCCATGTGA TCTGACACCC TGAGTAGTTC ACACCAGGCC CTAGGAGCTT
            781 CTCAGCCAGC GTTGTCAGCT GCTCGATGCT CAGCCCCCGC TTGGTGGTGG ACGAGAACTG
            841 CCAGCTGAGC ACCTCGGCCC ACTTGGTCCC AGGTTCCAAT TGGCGGCTTA GTGAAGAAGT
            901 TCACGTTCTT GGGGTTATTG GTCAGCATGT TATACCACAG GATTGATGCC CCAAGCATTT
            961 GGCATCTGAC AGATGTTGGA GATCACCACA ACTGGGCAAG GAGTGGGTCT CTAGGTCATC
           1021 TTTGAAGCCC TTGGTGGTAC ACCTCAGTCT CGGAAGGTGA TCAGGTGCAG CTCTCAGTCA
           1081 CGATTCAAGG AGGCATCACA ATTGCACGGC CTCCATTCCC ACATCTCTGC TCCCTTAGGG
          1141 CTCAAGTGCC TTGAACTCTG GCAGACACAG GCGTCGTCCC GCG
          //

          Please visit Sequencing and PCR primers for primer information.

          References

          Original, user report and related articles

          original Minami, M., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998.
          user_report Nakamura, H., An In Vivo Screening Model for Investigation of Pathophysiology of Human Implantation Failure. Biomolecules 13 (1): 79 (2022). PMID 36671464.
          user_report Shin, S.Y., Transcriptional regulation of the interleukin-11 gene by oncogenic Ras. Carcinogenesis, 33 (12): 2467-2476 (2012). PMID 23027619.
          user_report Chiba, T., Stat3 inhibition in neural lineage cells. Horm. Mol. Biol. Clin. Investig. 10 (2): 255-263 (2012). PMID 25436682.
          user_report Nakamura H., Mouse model of human infertility: transient and local inhibition of endometrial STAT-3 activation results in implantation failure. FEBS Lett., 580, 2717-2722 (2006). PMID 16647058.

          2023.05.22

          GNP_filter3_RDBDEP_html_230512.pl