Resource data sheet
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pEF-Flag-mSTAT3 (wild)

Expression vector of mouse STAT3

Catalog number RDB02353
Resource name pEF-Flag-mSTAT3 (wild)
Clone info. Expression vector for FLAG epitope-tagged mouse STAT3. 312-2621nt of NM_213659.3 (cds 309-2621) is cloned. Dominant negative form (pEF-Flag-mSTATY705F, RDB02354) is available.
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone ca. 5.2 kp
Selectable markers Amp^r
Gene/insert name Mouse STAT3 cDNA
Depositor Akira, Shizuo |
 
Other clones in our bank mouse Stat3 (NCBI Gene 20848) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02353 pEF-Flag-mSTAT3 (wild) DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB02353.gif from Depositor

Featured content

Featured content Dnaconda's recommendation EXR0003j (Japanese text)
Featured content Jak-STAT signaling pathway (English text)

References

original Minami, M., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14393_A6Kap1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: EF1a-C_F RDB14393_A6Kaa.seq
>02353_14393_A6Ka_EF1a-C_F_A05_02_ABI24.ab1
    1 TCATGGAATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT
   61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGGGTCG ACGGATTATG
  121 GACTACAAGG ACGACGATGA CAAGGCTCAG TGGAACCAGC TGCAGCAGCT GGACACACGC
  181 TACCTGGAGC AGCTGCACCA GCTGTACAGC GACAGCTTCC CCATGGAGCT GCGGCAGTTC
  241 CTGGCACCTT GGATTGAGAG TCAAGACTGG GCATATGCAG CCAGCAAAGA GTCACATGCC
  301 ACGTTGGTGT TTCATAATCT CTTGGGTGAA ATTGACCAGC AATATAGCCG ATTCCTGCAA
  361 GAGTCCAATG TCCTCTATCA GCACAACCTT CGAAGAATCA AGCAGTTTCT GCAGAGCAGG
  421 TATCTTGAGA AGCCAATGGA AATTGCCCGG ATCGTGGCCC GATGCCTGTG GGAAGAGTCT
  481 CGCCTCCTCC AGACGGCAGC CACGGCAGCC CAGCAAGGGG GCCAGGCCAA CCACCCAACA
  541 GCCGCCGTAG TGACAGAGAA GCAGCAGATG TTGGAGCAGC ATCTTCAGGA TGTCCGGAAG
  601 CGAGTGCAGG ATCTAGAACA GAAAATGAAG GTGGTGGAGA ACCTCCAGGA CGACTTTGAT
  661 TTCAACTACA AAACCCTCAA GAGCCAAGGA GACATGCAGG ATCTGAATGG AAACAACCAG
  721 TCTGTGACCA GACAGAAGAT GCAGCAGCTG GAACAGATGC TCACAGCCCT GGACCAGATG
  781 CGGAGAAGCA TTGTGAGTGA GCTGGCGGGG CTCTTGTCAG CAATGGAGTA CGTGCAGAAG
  841 ACACTGACTG ATGAAGAGCT GGCTGACTGG AAGAGGCGGC AGCAGATCGC GTGCATCGGA
  901 GGCCCTCCCA ACATCTGCCT GGACCGTCTG GAAAACTGGA TAACTTCATT AGCAGAATCT
  961 CAACTTCAGA CCCGCCAACA AATTAAGAAA CTGGAGGAGC TGCAGCAGAA AGTGTCCCTA
 1021 CAAGGGCGAC CCTATCGTGC AGCACCGGCC CATGCTGGAG AGAGATCGTG GCAGCTGGTC
 1081 AGAACTATTG AGAGTGCCTT CGTGTGAGCG CAGCCAGCAT GCTCAATGCA CCCGAACCCG
1141 GACCTTAAGT CA
//
Primer: hGCSF-R RDB14393_A6Kab.seq
>02353_14393_A6Ka_hGCSF-R_B05_05_ABI24.ab1
    1 TGGGGGAAAT GATACTCTCA AGGGTCCCAG GTGGGGACCC TCACTCTAGG GTCGACTCAC
   61 ATGGGGGAGG TAGCACACTC CGAGGTCAGA TCCATGTCAA ACGTGAGCGA CTCAAACTGC
  121 CCTCCTGCTG AGGGCTCAGC ACCTTCACCG TTATTTCCAA ACTGCATCAA TGAATCTAAA
  181 GTGCGGGGGG ACATCGGCAG GTCAATGGTA TTGCTGCAGG TCGTTGGTGT CACACAGATG
  241 AACTTGGTCT TCAGGTACGG GGCAGCACTA CCTGGGTCGG CTTCGGGGTG CTCCTGGCTC
  301 TCGGGCCTAC AGTACTTTCC AAATGCCTCC TCCTTGGGAA TGTCGGGGTA GAGGTAGACA
  361 AGTGGAGACA CCAGGATGTT GGTCGCATCC ATGATCTTAT AGCCCATGAT GATTTCAGCA
  421 AATGACATGT TGTTCAGCTG CTGCTTGGTG TATGGCTCTA CAGACTGGAT CTGGGTCTTG
  481 CCACTGATGT CCTTTTCCAC CCAAGTGAAA GTGACCCCTC CTTCTTTGCT GCTCTCGCTG
  541 AAGCGCAGTA GGAAGGTGCC CGGGGGCTTT GTGCTTAGGA TGGCCCGCTC CCGCTCCTTG
  601 CTGATGAAAC CCATGATGTA CCCTTCATTC CAAAGGGCCA AGATATACTT TTTCACAAGG
  661 TCGATGATAT TGTCTAGCCA GACCCAGAAG GAGAAGCCCT TGCCAGCCAT GTTTTCTTTG
  721 CAGAATTTAG CCCATGTGAT CTGACACCCT GAGTAGTTCA CACCAGGCCC TAGGAGCTTC
  781 TCAGCCAGCG TTGTCAGCTG CTCGATGCTC AGCCCCCGCT TGGTGGTGGA CGAGAACTGC
  841 CAGCTGAGCA CCTCGGCCAC TTGGTCCCAG GTTCCAATTG GCGGCTTAGT GAAGAAGTTC
  901 ACGTTCTTGG GGTTATTGGT CAGCATGTTA TACCACAGGA TTGATGCCCA AGCATTTGGC
  961 ATCTGACAGA TGTTGGAGAT CACCACAACT GGCAAGGAGT GGGTCTCTAG GTCAATCTTG
 1021 AGGCCTTTGG TGGTACACCT CAGTCTCGAA AGGTGATCAG GTGCAGCTCC TCAGTCACGA
 1081 TCAAGGAAGC ATCACATGGC ACGGCCTCCA TTCCCACATC TCTGGCTTCC TTAGTTCAAG
1141 GTGCATGAAC CTCTTGCAGA AACAGAGGGC TGGCCGGTTG GTTTAG
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Minami, M., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998.
user report Nagashima, H., The adaptor TRAF5 limits the differentiation of inflammatory CD4(+) T cells by antagonizing signaling via the receptor for IL-6. Nat. Immunol., 15 (5): 449-456 (2014). PMID 24681564.

2019.03.04

GNP_filter3_RDBDEP_html_190131.pl