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pEF-Flag-mSTAT3 (wild)

Expression vector of mouse STAT3

Catalog number RDB02353
Resource name pEF-Flag-mSTAT3 (wild)
Clone info. Expression vector for FLAG epitope-tagged mouse STAT3. 312-2621nt of NM_213659.3 (cds 309-2621) is cloned. Dominant negative form (pEF-Flag-mSTATY705F, RDB02354) is available.
Vector backbone pEF-BOS (Plasmid)
Size of vector backbone ca. 5.2 kp
Selectable markers Amp^r
Gene/insert name Mouse STAT3 cDNA
Depositor|Developer Akira, Shizuo |
Other clones in our bank

External Database
Mouse STAT3

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
          Additional terms and conditions for distribution Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
          付加的提供条件 pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB02353 pEF-Flag-mSTAT3 (wild) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB02353.gif

          Original reference

          original Minami, M., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998.

          Further references such as user reports and related articles (go to bottom)

          Featured content

          Featured content Dnaconda's recommendation EXR0003j (Japanese text)
          Featured content Jak-STAT signaling pathway (English text)

          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14393_A6Kap1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: EF1a-C_F
          Sequence file: RDB14393_A6Kaa.seq check
          >02353_14393_A6Ka_EF1a-C_F_A05_02_ABI24.ab1
              1 TCATGGAATT GCCTTTTTGA GTTTGGATCT TGGTTCATTC TCAAGCCTCA GACAGTGGTT
             61 CAAAGTTTTT TTCTTCCATT TCAGGTGTCG TGAGGAATTC TCTAGGGTCG ACGGATTATG
            121 GACTACAAGG ACGACGATGA CAAGGCTCAG TGGAACCAGC TGCAGCAGCT GGACACACGC
            181 TACCTGGAGC AGCTGCACCA GCTGTACAGC GACAGCTTCC CCATGGAGCT GCGGCAGTTC
            241 CTGGCACCTT GGATTGAGAG TCAAGACTGG GCATATGCAG CCAGCAAAGA GTCACATGCC
            301 ACGTTGGTGT TTCATAATCT CTTGGGTGAA ATTGACCAGC AATATAGCCG ATTCCTGCAA
            361 GAGTCCAATG TCCTCTATCA GCACAACCTT CGAAGAATCA AGCAGTTTCT GCAGAGCAGG
            421 TATCTTGAGA AGCCAATGGA AATTGCCCGG ATCGTGGCCC GATGCCTGTG GGAAGAGTCT
            481 CGCCTCCTCC AGACGGCAGC CACGGCAGCC CAGCAAGGGG GCCAGGCCAA CCACCCAACA
            541 GCCGCCGTAG TGACAGAGAA GCAGCAGATG TTGGAGCAGC ATCTTCAGGA TGTCCGGAAG
            601 CGAGTGCAGG ATCTAGAACA GAAAATGAAG GTGGTGGAGA ACCTCCAGGA CGACTTTGAT
            661 TTCAACTACA AAACCCTCAA GAGCCAAGGA GACATGCAGG ATCTGAATGG AAACAACCAG
            721 TCTGTGACCA GACAGAAGAT GCAGCAGCTG GAACAGATGC TCACAGCCCT GGACCAGATG
            781 CGGAGAAGCA TTGTGAGTGA GCTGGCGGGG CTCTTGTCAG CAATGGAGTA CGTGCAGAAG
            841 ACACTGACTG ATGAAGAGCT GGCTGACTGG AAGAGGCGGC AGCAGATCGC GTGCATCGGA
            901 GGCCCTCCCA ACATCTGCCT GGACCGTCTG GAAAACTGGA TAACTTCATT AGCAGAATCT
            961 CAACTTCAGA CCCGCCAACA AATTAAGAAA CTGGAGGAGC TGCAGCAGAA AGTGTCCCTA
           1021 CAAGGGCGAC CCTATCGTGC AGCACCGGCC CATGCTGGAG AGAGATCGTG GCAGCTGGTC
           1081 AGAACTATTG AGAGTGCCTT CGTGTGAGCG CAGCCAGCAT GCTCAATGCA CCCGAACCCG
          1141 GACCTTAAGT CA
          //
          Primer: hGCSF-R
          Sequence file: RDB14393_A6Kab.seq check
          >02353_14393_A6Ka_hGCSF-R_B05_05_ABI24.ab1
              1 TGGGGGAAAT GATACTCTCA AGGGTCCCAG GTGGGGACCC TCACTCTAGG GTCGACTCAC
             61 ATGGGGGAGG TAGCACACTC CGAGGTCAGA TCCATGTCAA ACGTGAGCGA CTCAAACTGC
            121 CCTCCTGCTG AGGGCTCAGC ACCTTCACCG TTATTTCCAA ACTGCATCAA TGAATCTAAA
            181 GTGCGGGGGG ACATCGGCAG GTCAATGGTA TTGCTGCAGG TCGTTGGTGT CACACAGATG
            241 AACTTGGTCT TCAGGTACGG GGCAGCACTA CCTGGGTCGG CTTCGGGGTG CTCCTGGCTC
            301 TCGGGCCTAC AGTACTTTCC AAATGCCTCC TCCTTGGGAA TGTCGGGGTA GAGGTAGACA
            361 AGTGGAGACA CCAGGATGTT GGTCGCATCC ATGATCTTAT AGCCCATGAT GATTTCAGCA
            421 AATGACATGT TGTTCAGCTG CTGCTTGGTG TATGGCTCTA CAGACTGGAT CTGGGTCTTG
            481 CCACTGATGT CCTTTTCCAC CCAAGTGAAA GTGACCCCTC CTTCTTTGCT GCTCTCGCTG
            541 AAGCGCAGTA GGAAGGTGCC CGGGGGCTTT GTGCTTAGGA TGGCCCGCTC CCGCTCCTTG
            601 CTGATGAAAC CCATGATGTA CCCTTCATTC CAAAGGGCCA AGATATACTT TTTCACAAGG
            661 TCGATGATAT TGTCTAGCCA GACCCAGAAG GAGAAGCCCT TGCCAGCCAT GTTTTCTTTG
            721 CAGAATTTAG CCCATGTGAT CTGACACCCT GAGTAGTTCA CACCAGGCCC TAGGAGCTTC
            781 TCAGCCAGCG TTGTCAGCTG CTCGATGCTC AGCCCCCGCT TGGTGGTGGA CGAGAACTGC
            841 CAGCTGAGCA CCTCGGCCAC TTGGTCCCAG GTTCCAATTG GCGGCTTAGT GAAGAAGTTC
            901 ACGTTCTTGG GGTTATTGGT CAGCATGTTA TACCACAGGA TTGATGCCCA AGCATTTGGC
            961 ATCTGACAGA TGTTGGAGAT CACCACAACT GGCAAGGAGT GGGTCTCTAG GTCAATCTTG
           1021 AGGCCTTTGG TGGTACACCT CAGTCTCGAA AGGTGATCAG GTGCAGCTCC TCAGTCACGA
           1081 TCAAGGAAGC ATCACATGGC ACGGCCTCCA TTCCCACATC TCTGGCTTCC TTAGTTCAAG
          1141 GTGCATGAAC CTCTTGCAGA AACAGAGGGC TGGCCGGTTG GTTTAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Minami, M., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998.
          user report Nagashima, H., The adaptor TRAF5 limits the differentiation of inflammatory CD4(+) T cells by antagonizing signaling via the receptor for IL-6. Nat. Immunol., 15 (5): 449-456 (2014). PMID 24681564.

          2022.02.09

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