Resource data sheet
DNA Bank Top
Please review the HTS results indicated by check icon below as well as clone information before placing your order.

pEF-Flag-mSTAT3 (wild) (#RDB02353)

Expression vector of mouse STAT3

Clone info. Expression vector for FLAG epitope-tagged mouse STAT3. 312-2621nt of NM_213659.3 (cds 309-2621) is cloned. Dominant negative form (pEF-Flag-mSTATY705F, RDB02354) is available.
Vector backbone pEF-BOS (RDB07939) (Plasmid)
Size of vector backbone ca. 5.2 kp
Selectable markers Amp^r
Growth conditions 37 C, LB+Amp
Gene/insert name Mouse STAT3 cDNA
Depositor|Developer Akira, Shizuo |
 
Sequence (full) RDB02353hts01.seq checkAssembled from experimentally sequenced data.
Additional map
Publication Minami, M., Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). [link to RRC of NBRP]
Test sheet RDB14393_A6Kap1-3.pdf 
 

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization.
Additional terms and conditions:
Regarding pEF-BOS and pEF-BOS-EX vectors: The RECIPIENT agrees to use the BIOLOGICAL RESOURCE only for academic research in the non-profit organization. RECIPIENT shall contact Dr. Shigekazu Nagata in the case of any commercial use of the pEF-BOS or pEF-BOS-EX vectors. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by Dr. Shigekazu Nagata (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990) and an acknowledgment to Dr. Shigekazu Nagata are requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。
付加的使用条件：
pEF-BOS and pEF-BOS-EXベクターについて: 本件リソースは非営利機関にのみ提供し、学術研究にのみ利用することができる。利用者が、pEF-BOSおよびpEF-BOS-EXベクターを商業的に利用する場合、利用者は長田重一教授に連絡するものとする。利用者は、研究成果の公表にあたって長田重一教授の指定する文献 (Mizushima S. and Nagata S.I, Nucleic Acids Res. 18: 5322, 1990.)を引用し、長田重一教授への謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02353 pEF-Flag-mSTAT3 (wild) DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

How to cite this biological resource

Materials & Methods section:

The pEF-Flag-mSTAT3 (wild) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02353).

Reference section:

Minami, M., Inoue, M., Wei, S., Takeda, K., Matsumoto, M., Kishimoto, T., Akira, S., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Minami, M., STAT3 activation is a critical step in gp130-mediated terminal differentiation and growth arrest of a myeloid cell line. Proc. Natl. Acad. Sci. USA, 93, 3963-3966 (1996). PMID 8632998. [link to RRC of NBRP]
user_report Nagashima, H., The adaptor TRAF5 limits the differentiation of inflammatory CD4(+) T cells by antagonizing signaling via the receptor for IL-6. Nat. Immunol., 15 (5): 449-456 (2014). PMID 24681564. [link to RRC of NBRP]