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D-Myc-pRc/CMV

Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter.

Catalog number RDB02141
Resource name D-Myc-pRc/CMV
Alternative name D-Myc, double Myc-tagged expression vector
Clone info. Expression vector. Expressing a fusion protein tagged with duplicated Myc epitopes under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor|Developer Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (DNA Cell Biol., 16, 893-896 (1997))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB02141 D-Myc-pRc/CMV DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB02141z.seq
            Remarks, protocol and/or map (gif) RDB02141.gif
            Remarks, protocol and/or map (pdf) RDB02141.pdf

            Original reference

            original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content pCMV_tag and pRSV_tag vector (English text)
            Featured content pCMV_tag and pRSV_tag vector (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13915_A6App1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV-Forward
            Sequence file: RDB13915_A6Apa.seq check
            >02141_13915_A6Ap_2_CMV-Forward_D06_12.ab1
                1 CTTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
               61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
              121 TCACTATAGG GAGACCCAAG CTTCCATGGA GCAGAAGCTG ATCAGCGAGG AGGACCTGGC
              181 GGCCGAGCAG AAGCTGATAA GCGAGGAGGA CCTGGCGGCC GCTCTAGAGG GCCCTATTCT
              241 ATAGTGTCAC CTAAATGCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA
              301 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC
              361 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT
              421 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA
              481 TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCGAG
              541 GGGGGATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
              601 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
              661 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
              721 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
              781 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
              841 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC
              901 TTTTGATTTA TAAAGGGATT TTGCCGATTT CGGCCTATTG GTTTAAAAAA TGAGCTGATT
              961 TAACAAAAAA TTTAACGCGA ATTTTAACAA ATATTTAACG CTTACATTTA AATATTTGCT
             1021 TATACAATCT TCCTGTTTTT GGGGCTTTTT CTGATTATCC AGCGGGGTGG GTTACGAAGC
            1081 TTCGAATCTG TGAATGTGTG TCAGGTAAGG GTGGTGGAAG CTCCCCCAAG GACATC
            //
            Primer: SV40pro_F_V2
            Sequence file: RDB13915_A6Apb.seq check
            >02141_13915_A6Ap_2_SV40pro_F_V2_G06_21.ab1
                1 TACGGCGTTT TTGGGAGCCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGG ATATCCATTT
               61 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
              121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
              181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
              241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT
              301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
              361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
              421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
              481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
              541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG
              601 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC
              661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
              721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA
              781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG
              841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC
              901 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC
              961 CACCGCCGCC TTTCTATGAA AGGTTTGGCT TCGGAAATCG TTTTCCGGGA CGCGGGCTGG
             1021 GATGATCCTC CCAGCGCGGG ATCTCATGCT GGAGTTCCTT CGCCACCCAC TGTTTATGCA
            1081 GCTATATGTT ACAATTAAGC CAGTAGCCAT CACCAAAATT TTT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

            2022.02.09

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