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D-Myc-pRc/CMV

Expressing a fusion protein tagged with duplicated Myc epitopes, CMV promoter.

Catalog number RDB02141
Resource name D-Myc-pRc/CMV
Alternative name D-Myc, double Myc-tagged expression vector
Clone info. Expression vector. Expressing a fusion protein tagged with duplicated Myc epitopes under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
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RDB02141 D-Myc-pRc/CMV DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02141z.seq from Depositor
Electronic file Map RDB02141.gif from Depositor
Electronic file PDF RDB02141.pdf from Depositor

Featured content

Featured content pCMV_tag and pRSV_tag vector (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)
Featured content pCMV_tag and pRSV_tag vector (Japanese text)

References

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13915_A6App1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13915_A6Apa.seq
>02141_13915_A6Ap_2_CMV-Forward_D06_12.ab1
    1 CTTCACTCCG CCCATTGACG CAATGGGCGG TAGGCGTGTA CGGTGGGAGG TCTATATAAG
   61 CAGAGCTCTC TGGCTAACTA GAGAACCCAC TGCTTACTGG CTTATCGAAA TTAATACGAC
  121 TCACTATAGG GAGACCCAAG CTTCCATGGA GCAGAAGCTG ATCAGCGAGG AGGACCTGGC
  181 GGCCGAGCAG AAGCTGATAA GCGAGGAGGA CCTGGCGGCC GCTCTAGAGG GCCCTATTCT
  241 ATAGTGTCAC CTAAATGCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA
  301 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC
  361 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT
  421 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA
  481 TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCGAG
  541 GGGGGATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
  601 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
  661 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
  721 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
  781 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
  841 CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC
  901 TTTTGATTTA TAAAGGGATT TTGCCGATTT CGGCCTATTG GTTTAAAAAA TGAGCTGATT
  961 TAACAAAAAA TTTAACGCGA ATTTTAACAA ATATTTAACG CTTACATTTA AATATTTGCT
 1021 TATACAATCT TCCTGTTTTT GGGGCTTTTT CTGATTATCC AGCGGGGTGG GTTACGAAGC
1081 TTCGAATCTG TGAATGTGTG TCAGGTAAGG GTGGTGGAAG CTCCCCCAAG GACATC
//
Primer: SV40pro_F_V2 RDB13915_A6Apb.seq
>02141_13915_A6Ap_2_SV40pro_F_V2_G06_21.ab1
    1 TACGGCGTTT TTGGGAGCCT AGGCTTTTGC AAAAGCTCCC GGGAGCTTGG ATATCCATTT
   61 TCGGATCTGA TCAAGAGACA GGATGAGGAT CGTTTCGCAT GATTGAACAA GATGGATTGC
  121 ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA GGCTATTCGG CTATGACTGG GCACAACAGA
  181 CAATCGGCTG CTCTGATGCC GCCGTGTTCC GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT
  241 TTGTCAAGAC CGACCTGTCC GGTGCCCTGA ATGAACTGCA GGACGAGGCA GCGCGGCTAT
  301 CGTGGCTGGC CACGACGGGC GTTCCTTGCG CAGCTGTGCT CGACGTTGTC ACTGAAGCGG
  361 GAAGGGACTG GCTGCTATTG GGCGAAGTGC CGGGGCAGGA TCTCCTGTCA TCTCACCTTG
  421 CTCCTGCCGA GAAAGTATCC ATCATGGCTG ATGCAATGCG GCGGCTGCAT ACGCTTGATC
  481 CGGCTACCTG CCCATTCGAC CACCAAGCGA AACATCGCAT CGAGCGAGCA CGTACTCGGA
  541 TGGAAGCCGG TCTTGTCGAT CAGGATGATC TGGACGAAGA GCATCAGGGG CTCGCGCCAG
  601 CCGAACTGTT CGCCAGGCTC AAGGCGCGCA TGCCCGACGG CGAGGATCTC GTCGTGACCC
  661 ATGGCGATGC CTGCTTGCCG AATATCATGG TGGAAAATGG CCGCTTTTCT GGATTCATCG
  721 ACTGTGGCCG GCTGGGTGTG GCGGACCGCT ATCAGGACAT AGCGTTGGCT ACCCGTGATA
  781 TTGCTGAAGA GCTTGGCGGC GAATGGGCTG ACCGCTTCCT CGTGCTTTAC GGTATCGCCG
  841 CTCCCGATTC GCAGCGCATC GCCTTCTATC GCCTTCTTGA CGAGTTCTTC TGAGCGGGAC
  901 TCTGGGGTTC GAAATGACCG ACCAAGCGAC GCCCAACCTG CCATCACGAG ATTTCGATTC
  961 CACCGCCGCC TTTCTATGAA AGGTTTGGCT TCGGAAATCG TTTTCCGGGA CGCGGGCTGG
 1021 GATGATCCTC CCAGCGCGGG ATCTCATGCT GGAGTTCCTT CGCCACCCAC TGTTTATGCA
1081 GCTATATGTT ACAATTAAGC CAGTAGCCAT CACCAAAATT TTT
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

2018.12.11

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