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S-Myc-pRc/CMV

Expressing Myc-tagged fusion protein, CMV promoter.

Catalog number RDB02140
Resource name S-Myc-pRc/CMV
Alternative name S-Myc, single Myc-tagged expression vector
Clone info. Expression vector. Expressing Myc-tagged fusion protein under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor|Developer Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |
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            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (DNA Cell Biol., 16, 893-896 (1997))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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            RDB02140 S-Myc-pRc/CMV DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB02140z.seq
            Remarks, protocol and/or map (gif) RDB02140.gif
            Remarks, protocol and/or map (pdf) RDB02140.pdf

            Original reference

            original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content pCMV_tag and pRSV_tag vector (English text)
            Featured content pCMV_tag and pRSV_tag vector (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14003_A6Csp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV-Forward
            Sequence file: RDB14003_A6Csa.seq check
            >02140_14003_A6Cs_1_CMV-Forward_C01_07.ab1
                1 ACGTTCCAAC CTCCCGCCCC ATTGACGGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT
               61 CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT
              121 TAATACGACT CACTATAGGG AGACCCAAGC TTCCATGGAG CAGAAGCTGA TCAGCGAGGA
              181 GGACCTGGCG GCCGCTCGAG CATGCATCTA GAGGGCCCTA TTCTATAGTG TCACCTAAAT
              241 GCTAGAGCTC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC
              301 CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA
              361 AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG
              421 GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG
              481 GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CGAGGGGGGA TCCCCACGCG
              541 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA
              601 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC
              661 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT
              721 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG
              781 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC
              841 TTGTTCCAAA ACTGGGAACA ACACTCAACC CTATCTCGGG TCTATTCTTT TGATTTATAA
              901 GGGATTTTGC CGATTTCGGC CTATTGGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA
              961 CGCGAAATTT AACAAAATAT TAACGCTTAC AAATTAATTA TTTGCTTATA CAATCTTCCC
             1021 TGGTTTTTGG GGGCTTTTTC TGATTATCAC CGGGGGGTGG GGTACCGAGC TCGGAATTCT
             1081 GGTGGGAATG GTGGTGTCAG TTTAAGGGTG TGGAAAAGTC CCCCAGCTCC CCAGCAGGCA
            1141 GAGGTTATGC AAAGCATGCC AATCCTCCCA TTAGGTCCGA CGCAGAACAC TCGAGAG
            //
            Primer: SV40pro_F_V2
            Sequence file: RDB14003_A6Csb.seq check
            >02140_14003_A6Cs_1_SV40pro_F_V2_D01_10.ab1
                1 TTCCCCTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGGATA TCCATTTTCG
               61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG
              121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA
              181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG
              241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT
              301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA
              361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC
              421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG
              481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG
              541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG
              601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG
              661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT
              721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG
              781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC
              841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT
              901 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC
              961 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GGCTGGGATG
             1021 ATCCTCCAGC GCGGGGATCT CATGCTGAGG TTCTTCGCCC ACCCCCAACT TGTTTATTGC
             1081 AGCTTATAAT GGCTTTACGA TAAGGCCATA GCCATTCACT AATTTCACAA ATTAAAGCAT
            1141 TTTTTTTTCC ACCTGGCCAA TC
            //

            Please visit Sequencing and PCR primers for primer information.

            References

            Original, user report and related articles

            original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

            2022.02.09

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