Expressing Myc-tagged fusion protein, CMV promoter.
| Catalog number | RDB02140 |
|---|---|
| Resource name | S-Myc-pRc/CMV |
| Alternative name | S-Myc, single Myc-tagged expression vector |
| Clone info. | Expression vector. Expressing Myc-tagged fusion protein under the control of CMV promoter. |
| Vector backbone | pRc/CMV (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Depositor | Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB02140 | S-Myc-pRc/CMV | DNA solution | Please contact us. |
Electronic file
| Electronic file | Sequence RDB02140z.seq from Depositor |
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| Electronic file | Map RDB02140.gif from Depositor
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| Electronic file | Remarks, protocol and/or map (pdf) RDB02140.pdf from Depositor |
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Empty Backbone (English text) |
|---|---|
| Featured content | Empty Backbone (Japanese text) |
| Featured content | pCMV_tag and pRSV_tag vector (English text) |
| Featured content | pCMV_tag and pRSV_tag vector (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB14003_A6Csp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | Sequence: RDB14003_A6Csa.seq |
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>02140_14003_A6Cs_1_CMV-Forward_C01_07.ab1 1 ACGTTCCAAC CTCCCGCCCC ATTGACGGCA AATGGGCGGT AGGCGTGTAC GGTGGGAGGT 61 CTATATAAGC AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT 121 TAATACGACT CACTATAGGG AGACCCAAGC TTCCATGGAG CAGAAGCTGA TCAGCGAGGA 181 GGACCTGGCG GCCGCTCGAG CATGCATCTA GAGGGCCCTA TTCTATAGTG TCACCTAAAT 241 GCTAGAGCTC GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC 301 CCCTCCCCCG TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA 361 AATGAGGAAA TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG 421 GGGCAGGACA GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG 481 GGCTCTATGG CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CGAGGGGGGA TCCCCACGCG 541 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 601 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 661 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 721 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 781 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 841 TTGTTCCAAA ACTGGGAACA ACACTCAACC CTATCTCGGG TCTATTCTTT TGATTTATAA 901 GGGATTTTGC CGATTTCGGC CTATTGGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA 961 CGCGAAATTT AACAAAATAT TAACGCTTAC AAATTAATTA TTTGCTTATA CAATCTTCCC 1021 TGGTTTTTGG GGGCTTTTTC TGATTATCAC CGGGGGGTGG GGTACCGAGC TCGGAATTCT 1081 GGTGGGAATG GTGGTGTCAG TTTAAGGGTG TGGAAAAGTC CCCCAGCTCC CCAGCAGGCA 1141 GAGGTTATGC AAAGCATGCC AATCCTCCCA TTAGGTCCGA CGCAGAACAC TCGAGAG // |
| Primer: SV40pro_F_V2 | Sequence: RDB14003_A6Csb.seq |
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>02140_14003_A6Cs_1_SV40pro_F_V2_D01_10.ab1 1 TTCCCCTTTT TGGAGCTAGG CTTTTGCAAA AGCTCCCGGG AGCTTGGATA TCCATTTTCG 61 GATCTGATCA AGAGACAGGA TGAGGATCGT TTCGCATGAT TGAACAAGAT GGATTGCACG 121 CAGGTTCTCC GGCCGCTTGG GTGGAGAGGC TATTCGGCTA TGACTGGGCA CAACAGACAA 181 TCGGCTGCTC TGATGCCGCC GTGTTCCGGC TGTCAGCGCA GGGGCGCCCG GTTCTTTTTG 241 TCAAGACCGA CCTGTCCGGT GCCCTGAATG AACTGCAGGA CGAGGCAGCG CGGCTATCGT 301 GGCTGGCCAC GACGGGCGTT CCTTGCGCAG CTGTGCTCGA CGTTGTCACT GAAGCGGGAA 361 GGGACTGGCT GCTATTGGGC GAAGTGCCGG GGCAGGATCT CCTGTCATCT CACCTTGCTC 421 CTGCCGAGAA AGTATCCATC ATGGCTGATG CAATGCGGCG GCTGCATACG CTTGATCCGG 481 CTACCTGCCC ATTCGACCAC CAAGCGAAAC ATCGCATCGA GCGAGCACGT ACTCGGATGG 541 AAGCCGGTCT TGTCGATCAG GATGATCTGG ACGAAGAGCA TCAGGGGCTC GCGCCAGCCG 601 AACTGTTCGC CAGGCTCAAG GCGCGCATGC CCGACGGCGA GGATCTCGTC GTGACCCATG 661 GCGATGCCTG CTTGCCGAAT ATCATGGTGG AAAATGGCCG CTTTTCTGGA TTCATCGACT 721 GTGGCCGGCT GGGTGTGGCG GACCGCTATC AGGACATAGC GTTGGCTACC CGTGATATTG 781 CTGAAGAGCT TGGCGGCGAA TGGGCTGACC GCTTCCTCGT GCTTTACGGT ATCGCCGCTC 841 CCGATTCGCA GCGCATCGCC TTCTATCGCC TTCTTGACGA GTTCTTCTGA GCGGGACTCT 901 GGGGTTCGAA ATGACCGACC AAGCGACGCC CAACCTGCCA TCACGAGATT TCGATTCCAC 961 CGCCGCCTTC TATGAAAGGT TGGGCTTCGG AATCGTTTTC CGGGACGCCG GGCTGGGATG 1021 ATCCTCCAGC GCGGGGATCT CATGCTGAGG TTCTTCGCCC ACCCCCAACT TGTTTATTGC 1081 AGCTTATAAT GGCTTTACGA TAAGGCCATA GCCATTCACT AATTTCACAA ATTAAAGCAT 1141 TTTTTTTTCC ACCTGGCCAA TC // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.11.02
GNP_filter3_RDBDEP_html_190927.pl