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D-HA-pRc/CMV (#RDB02139)

Expressing a fusion protein tagged with duplicated HA, CMV promoter.


Drawn by SnapGene® software
Sequence information
(Assembled from experimentally sequenced data)		 GenBank Flat File Format open
SnapGene file download
Publication Takemoto, Y., DNA Cell Biol., 16, 893-896 (1997). PMID 9260932. [PubMed] [Article] [RRC of NBRP]
Test sheet Data Sheet open 
 
Alternative name D-HA, double HA-tagged expression vector
Clone info. Expression vector. Expressing a fusion protein tagged with duplicated HA under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Growth conditions 37 C, LB+Amp
Depositor|Developer Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |

Distribution information

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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (DNA Cell Biol., 16, 893-896 (1997))。

Catalog # Resource name Shipping form Fee
RDB02139 D-HA-pRc/CMV DNA solution

How to cite this biological resource

Materials & Methods section:

The D-HA-pRc/CMV was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02139).

Reference section:

Takemoto, Y., Sato, M., Furuta, M., Hashimoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932. [PubMed] [Article] [RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

References

Original, user report and related articles

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932. [PubMed] [Article] [RRC of NBRP]
user_report Mitsui, S., Mental retardation-related protease, motopsin (prss12), binds to the BRICHOS domain of the integral membrane protein 2a. Cell Biol. Int., 38 (1): 117-123 (2014). PMID 23955961. [PubMed] [Article] [RRC of NBRP]
user_report Miura, E., VPS35 dysfunction impairs lysosomal degradation of alpha-synuclein and exacerbates neurotoxicity in a Drosophila model of Parkinson's disease. Neurobiol. Dis. 71: 1-13 (2014). PMID 25107340. [PubMed] [Article] [RRC of NBRP]
user_report Mitsui, S., A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6. Biochem. Biophys. Res. Commun., 436 (4): 638-644 (2013). PMID 23770366. [PubMed] [Article] [RRC of NBRP]
user_report Hayashi, H., Regulation of intestinal Cl-/HCO3- exchanger SLC26A3 by intracellular pH. Am. J. Physiol. Cell Physiol., 296, C1279-1290 (2009). PMID 19321737. [PubMed] [Article] [RRC of NBRP]