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D-HA-pRc/CMV

Expressing a fusion protein tagged with duplicated HA, CMV promoter.

Catalog number RDB02139
Resource name D-HA-pRc/CMV
Alternative name D-HA, double HA-tagged expression vector
Clone info. Expression vector. Expressing a fusion protein tagged with duplicated HA under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02139 D-HA-pRc/CMV DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02139z.seq from Depositor
Electronic file Map RDB02139.gif from Depositor
Electronic file Remarks, protocol and/or map (pdf) RDB02139.pdf from Depositor

Original reference

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)
Featured content pCMV_tag and pRSV_tag vector (English text)
Featured content pCMV_tag and pRSV_tag vector (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14667_A7D6p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward | Sequence: RDB14667_A7D6a.seq
>02139_14667_A7D6_CMV-Forward_A02_01_ABI08.ab1
    1 TGTACCAACT CCGCCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
   61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
  121 TACGACTCAC TATAGGGAGA CCCAAGCTTC CATGGCCTAC CCCTACGACG TGCCCGACTA
  181 CGCCGCGGCC TACCCCTACG ACGTGCCCGA CTACGCGGCC GCTCTAGAGG GCCCTATTCT
  241 ATAGTGTCAC CTAAATGCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA
  301 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC
  361 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT
  421 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA
  481 TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCGAG
  541 GGGGGATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
  601 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
  661 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
  721 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
  781 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
  841 CTTTAATAGT GGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT
  901 CTTTTGATTT ATAAGGGATT TTTGCCGATT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT
  961 TTTAACAAAA ATTTTAACGC GAATTTTAAC AAAATATTTA ACGCCTTACC AATTAAATTA
 1021 TTTGCTTTAA TACCAATTCT TTCCTGTTTT GGGGCTTTTC TGATTAATCC AAACCCGGGG
 1081 TGGGTACCGA GGCTTCGAAA TCCTGTGATG GTGTGTCAGT TAGGGGTGTG GGAAAGGTTC
1141 CCCACAGGAC TC
//
Primer: SV40pro_F_V2 | Sequence: RDB14667_A7D6b.seq
>02139_14667_A7D6_SV40pro_F_V2_B02_02_ABI08.ab1
    1 GGTCAGGGGT TTTTTTTGGA GGCCTAGGGC TTTTGCAAAA GCTCCCGGGC CCCTTGAGAT
   61 ATCCATTTTC GGATCTGATC AAAAAAAAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA
  121 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC
  181 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC
  241 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC
  301 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC
  361 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC
  421 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC
  481 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG
  541 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT
  601 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT
  661 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG
  721 ATTCATCGAC TGTGGCCGGC TGGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA
  781 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG
  841 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT
  901 GAGCGGGACT CTGGGGGTTT CGAAATGACC GACCCAAGCG ACGCCCCAAC CTGCCCATCA
  961 CGAGATTTTC GAATTTCCAC CGCCGCCTTC TATGGAAAGG GTTGGGCTTT CGGAAATCGT
 1021 TTCCCGGGAC GCCCGGGCTG GAATGGAATC CTTCCAAGCG CCGGGGAATC TTCAATGCCT
 1081 GGAGATTTCC TTTCGCCCCA ACCTCGAAAC TTGGTAATTG GCCAGGCTTA TAATGGGGTT
1141 TACCAAATAT AAAAGGCAAA TAAGGCAACT CCACGCCCGG A
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.
user report Mitsui, S., Mental retardation-related protease, motopsin (prss12), binds to the BRICHOS domain of the integral membrane protein 2a. Cell Biol. Int., 38 (1): 117-123 (2014). PMID 23955961.
user report Miura, E., VPS35 dysfunction impairs lysosomal degradation of alpha-synuclein and exacerbates neurotoxicity in a Drosophila model of Parkinson's disease. Neurobiol. Dis. 71: 1-13 (2014). PMID 25107340.
user report Mitsui, S., A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6. Biochem. Biophys. Res. Commun., 436 (4): 638-644 (2013). PMID 23770366.
user report Hayashi, H., Regulation of intestinal Cl-/HCO3- exchanger SLC26A3 by intracellular pH. Am. J. Physiol. Cell Physiol., 296, C1279-1290 (2009). PMID 19321737.

2019.11.02

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