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D-HA-pRc/CMV

Expressing a fusion protein tagged with duplicated HA, CMV promoter.

Catalog number RDB02139
Resource name D-HA-pRc/CMV
Alternative name D-HA, double HA-tagged expression vector
Clone info. Expression vector. Expressing a fusion protein tagged with duplicated HA under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor|Developer Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (DNA Cell Biol., 16, 893-896 (1997))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

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            RDB02139 D-HA-pRc/CMV DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB02139z.seq
            Remarks, protocol and/or map (gif) RDB02139.gif
            Remarks, protocol and/or map (pdf) RDB02139.pdf

            Original reference

            original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content pCMV_tag and pRSV_tag vector (English text)
            Featured content pCMV_tag and pRSV_tag vector (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB14667_A7D6p1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV-Forward
            Sequence file: RDB14667_A7D6a.seq check
            >02139_14667_A7D6_CMV-Forward_A02_01_ABI08.ab1
                1 TGTACCAACT CCGCCCCCAT TGACGCAAAT GGGCGGTAGG CGTGTACGGT GGGAGGTCTA
               61 TATAAGCAGA GCTCTCTGGC TAACTAGAGA ACCCACTGCT TACTGGCTTA TCGAAATTAA
              121 TACGACTCAC TATAGGGAGA CCCAAGCTTC CATGGCCTAC CCCTACGACG TGCCCGACTA
              181 CGCCGCGGCC TACCCCTACG ACGTGCCCGA CTACGCGGCC GCTCTAGAGG GCCCTATTCT
              241 ATAGTGTCAC CTAAATGCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT CTAGTTGCCA
              301 GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG CCACTCCCAC
              361 TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT GTCATTCTAT
              421 TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA ATAGCAGGCA
              481 TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT GGGGCTCGAG
              541 GGGGGATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG
              601 CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC
              661 CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG
              721 GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC
              781 ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT
              841 CTTTAATAGT GGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT
              901 CTTTTGATTT ATAAGGGATT TTTGCCGATT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT
              961 TTTAACAAAA ATTTTAACGC GAATTTTAAC AAAATATTTA ACGCCTTACC AATTAAATTA
             1021 TTTGCTTTAA TACCAATTCT TTCCTGTTTT GGGGCTTTTC TGATTAATCC AAACCCGGGG
             1081 TGGGTACCGA GGCTTCGAAA TCCTGTGATG GTGTGTCAGT TAGGGGTGTG GGAAAGGTTC
            1141 CCCACAGGAC TC
            //
            Primer: SV40pro_F_V2
            Sequence file: RDB14667_A7D6b.seq check
            >02139_14667_A7D6_SV40pro_F_V2_B02_02_ABI08.ab1
                1 GGTCAGGGGT TTTTTTTGGA GGCCTAGGGC TTTTGCAAAA GCTCCCGGGC CCCTTGAGAT
               61 ATCCATTTTC GGATCTGATC AAAAAAAAGG ATGAGGATCG TTTCGCATGA TTGAACAAGA
              121 TGGATTGCAC GCAGGTTCTC CGGCCGCTTG GGTGGAGAGG CTATTCGGCT ATGACTGGGC
              181 ACAACAGACA ATCGGCTGCT CTGATGCCGC CGTGTTCCGG CTGTCAGCGC AGGGGCGCCC
              241 GGTTCTTTTT GTCAAGACCG ACCTGTCCGG TGCCCTGAAT GAACTGCAGG ACGAGGCAGC
              301 GCGGCTATCG TGGCTGGCCA CGACGGGCGT TCCTTGCGCA GCTGTGCTCG ACGTTGTCAC
              361 TGAAGCGGGA AGGGACTGGC TGCTATTGGG CGAAGTGCCG GGGCAGGATC TCCTGTCATC
              421 TCACCTTGCT CCTGCCGAGA AAGTATCCAT CATGGCTGAT GCAATGCGGC GGCTGCATAC
              481 GCTTGATCCG GCTACCTGCC CATTCGACCA CCAAGCGAAA CATCGCATCG AGCGAGCACG
              541 TACTCGGATG GAAGCCGGTC TTGTCGATCA GGATGATCTG GACGAAGAGC ATCAGGGGCT
              601 CGCGCCAGCC GAACTGTTCG CCAGGCTCAA GGCGCGCATG CCCGACGGCG AGGATCTCGT
              661 CGTGACCCAT GGCGATGCCT GCTTGCCGAA TATCATGGTG GAAAATGGCC GCTTTTCTGG
              721 ATTCATCGAC TGTGGCCGGC TGGGGTGTGG CGGACCGCTA TCAGGACATA GCGTTGGCTA
              781 CCCGTGATAT TGCTGAAGAG CTTGGCGGCG AATGGGCTGA CCGCTTCCTC GTGCTTTACG
              841 GTATCGCCGC TCCCGATTCG CAGCGCATCG CCTTCTATCG CCTTCTTGAC GAGTTCTTCT
              901 GAGCGGGACT CTGGGGGTTT CGAAATGACC GACCCAAGCG ACGCCCCAAC CTGCCCATCA
              961 CGAGATTTTC GAATTTCCAC CGCCGCCTTC TATGGAAAGG GTTGGGCTTT CGGAAATCGT
             1021 TTCCCGGGAC GCCCGGGCTG GAATGGAATC CTTCCAAGCG CCGGGGAATC TTCAATGCCT
             1081 GGAGATTTCC TTTCGCCCCA ACCTCGAAAC TTGGTAATTG GCCAGGCTTA TAATGGGGTT
            1141 TACCAAATAT AAAAGGCAAA TAAGGCAACT CCACGCCCGG A
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.
            user report Mitsui, S., Mental retardation-related protease, motopsin (prss12), binds to the BRICHOS domain of the integral membrane protein 2a. Cell Biol. Int., 38 (1): 117-123 (2014). PMID 23955961.
            user report Miura, E., VPS35 dysfunction impairs lysosomal degradation of alpha-synuclein and exacerbates neurotoxicity in a Drosophila model of Parkinson's disease. Neurobiol. Dis. 71: 1-13 (2014). PMID 25107340.
            user report Mitsui, S., A mental retardation gene, motopsin/prss12, modulates cell morphology by interaction with seizure-related gene 6. Biochem. Biophys. Res. Commun., 436 (4): 638-644 (2013). PMID 23770366.
            user report Hayashi, H., Regulation of intestinal Cl-/HCO3- exchanger SLC26A3 by intracellular pH. Am. J. Physiol. Cell Physiol., 296, C1279-1290 (2009). PMID 19321737.

            2022.02.09

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