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D-T7-pRc/CMV

Expressing a fusion protein tagged with duplicated T7, CMV promoter.

Catalog number RDB02138
Resource name D-T7-pRc/CMV
Alternative name D-T7, double T7-tagged expression vector
Clone info. Expression vector. Expressing a fusion protein tagged with duplicated T7 under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor|Developer Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |
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External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
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            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (DNA Cell Biol., 16, 893-896 (1997))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
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            RDB02138 D-T7-pRc/CMV DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB02138z.seq
            Remarks, protocol and/or map (gif) RDB02138.gif
            Remarks, protocol and/or map (pdf) RDB02138.pdf

            Original reference

            original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content pCMV_tag and pRSV_tag vector (English text)
            Featured content pCMV_tag and pRSV_tag vector (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13053_A5A5.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV-Forward
            Sequence file: RDB13053_A5A5a.seq check
            >02138_13053_A5A5_3_CMV-Forward_H02_23.ab1
                1 CCAATCACCT TCGGCCCCCA TTGACGCAAT GGGCGGTAGG CGTGTACGGC TGGGAGGTCT
               61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
              121 ATACGACTCA CTATAGGGAG ACCCAAGCTT CCATGGCCAG CATGACCGGC GGCCAGCAGA
              181 TGGGCGCGGC CATGGCCAGC ATGACCGGCG GCCAGCAGAT GGGCGCGGCC GCTCTAGAGG
              241 GCCCTATTCT ATAGTGTCAC CTAAATGCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT
              301 CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG
              361 CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT
              421 GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA
              481 ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT
              541 GGGGCTCGAG GGGGGATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG
              601 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT
              661 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC
              721 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG
              781 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG
              841 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT
              901 CGGTCTATTC TTTTGATTTA TAAAGGGATT TTGCCGATTT CGGCCTATTG GTTTAAAAAA
              961 TGAGCTGATT TAACAAAAAT TTAACGCGAA ATTTTAACAA AATATTAACG CTTTACAATT
             1021 TAATATTTGC TTATACAATC TTTCCTGTTT TTTGGGGCTT TTCTGATTAT CACCGGGTGG
             1081 TACGAGCTCG ATTCTTGTGA ATGTTGTGTC AGTAGGATGT GAAGTTCCTC AGGCCTCCCC
            1141 AGCAGGGCAG AAGTTATATG GCCCA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

            2022.02.09

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