Resource data sheet
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D-T7-pRc/CMV

Expressing a fusion protein tagged with duplicated T7, CMV promoter.

Catalog number RDB02138
Resource name D-T7-pRc/CMV
Alternative name D-T7, double T7-tagged expression vector
Clone info. Expression vector. Expressing a fusion protein tagged with duplicated T7 under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
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RDB02138 D-T7-pRc/CMV DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02138z.seq from Depositor
Electronic file Map RDB02138.gif from Depositor
Electronic file PDF RDB02138.pdf from Depositor

Featured content

Featured content pCMV_tag and pRSV_tag vector (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)
Featured content pCMV_tag and pRSV_tag vector (Japanese text)

References

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13053_A5A5.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13053_A5A5a.seq
>02138_13053_A5A5_3_CMV-Forward_H02_23.ab1
    1 CCAATCACCT TCGGCCCCCA TTGACGCAAT GGGCGGTAGG CGTGTACGGC TGGGAGGTCT
   61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA
  121 ATACGACTCA CTATAGGGAG ACCCAAGCTT CCATGGCCAG CATGACCGGC GGCCAGCAGA
  181 TGGGCGCGGC CATGGCCAGC ATGACCGGCG GCCAGCAGAT GGGCGCGGCC GCTCTAGAGG
  241 GCCCTATTCT ATAGTGTCAC CTAAATGCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT
  301 CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG
  361 CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT
  421 GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA
  481 ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT
  541 GGGGCTCGAG GGGGGATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG
  601 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT
  661 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC
  721 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG
  781 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG
  841 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT
  901 CGGTCTATTC TTTTGATTTA TAAAGGGATT TTGCCGATTT CGGCCTATTG GTTTAAAAAA
  961 TGAGCTGATT TAACAAAAAT TTAACGCGAA ATTTTAACAA AATATTAACG CTTTACAATT
 1021 TAATATTTGC TTATACAATC TTTCCTGTTT TTTGGGGCTT TTCTGATTAT CACCGGGTGG
 1081 TACGAGCTCG ATTCTTGTGA ATGTTGTGTC AGTAGGATGT GAAGTTCCTC AGGCCTCCCC
1141 AGCAGGGCAG AAGTTATATG GCCCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

2018.12.10

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