Expressing a fusion protein tagged with duplicated T7, CMV promoter.
| Catalog number | RDB02138 |
|---|---|
| Resource name | D-T7-pRc/CMV |
| Alternative name | D-T7, double T7-tagged expression vector |
| Clone info. | Expression vector. Expressing a fusion protein tagged with duplicated T7 under the control of CMV promoter. |
| Vector backbone | pRc/CMV (Plasmid) |
| Size of vector backbone | 5.5 kb |
| Selectable markers | Amp^r (E. coli), Neo^r (mammalian cell) |
| Depositor | Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru | |
Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
| Terms and conditions set forth by the DEPOSITOR | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)). |
|---|---|
| Ordering | Please visit Information of Request for Distribution. Order form [Credit Card Payment] [Bank Transfer or Check Payment] Material Transfer Agreement (MTA) [Word] |
[open/close]| Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
|---|---|---|---|
| RDB02138 | D-T7-pRc/CMV | DNA solution | Please contact us. |
Electronic file
| Electronic file | Sequence RDB02138z.seq from Depositor |
|---|---|
| Electronic file | Map RDB02138.gif from Depositor
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| Electronic file | Remarks, protocol and/or map (pdf) RDB02138.pdf from Depositor |
Original reference
Further references such as user reports and related articles (go to bottom)
Featured content
| Featured content | Empty Backbone (English text) |
|---|---|
| Featured content | Empty Backbone (Japanese text) |
| Featured content | pCMV_tag and pRSV_tag vector (English text) |
| Featured content | pCMV_tag and pRSV_tag vector (Japanese text) |
RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.
Data are summerized on test sheet below.
| Test sheet | RDB13053_A5A5.pdf |
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Nucleotide sequence of a portion of this resource (if available).
| Primer: CMV-Forward | Sequence: RDB13053_A5A5a.seq |
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>02138_13053_A5A5_3_CMV-Forward_H02_23.ab1 1 CCAATCACCT TCGGCCCCCA TTGACGCAAT GGGCGGTAGG CGTGTACGGC TGGGAGGTCT 61 ATATAAGCAG AGCTCTCTGG CTAACTAGAG AACCCACTGC TTACTGGCTT ATCGAAATTA 121 ATACGACTCA CTATAGGGAG ACCCAAGCTT CCATGGCCAG CATGACCGGC GGCCAGCAGA 181 TGGGCGCGGC CATGGCCAGC ATGACCGGCG GCCAGCAGAT GGGCGCGGCC GCTCTAGAGG 241 GCCCTATTCT ATAGTGTCAC CTAAATGCTA GAGCTCGCTG ATCAGCCTCG ACTGTGCCTT 301 CTAGTTGCCA GCCATCTGTT GTTTGCCCCT CCCCCGTGCC TTCCTTGACC CTGGAAGGTG 361 CCACTCCCAC TGTCCTTTCC TAATAAAATG AGGAAATTGC ATCGCATTGT CTGAGTAGGT 421 GTCATTCTAT TCTGGGGGGT GGGGTGGGGC AGGACAGCAA GGGGGAGGAT TGGGAAGACA 481 ATAGCAGGCA TGCTGGGGAT GCGGTGGGCT CTATGGCTTC TGAGGCGGAA AGAACCAGCT 541 GGGGCTCGAG GGGGGATCCC CACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG 601 TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT 661 TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC 721 TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG 781 GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG 841 AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT 901 CGGTCTATTC TTTTGATTTA TAAAGGGATT TTGCCGATTT CGGCCTATTG GTTTAAAAAA 961 TGAGCTGATT TAACAAAAAT TTAACGCGAA ATTTTAACAA AATATTAACG CTTTACAATT 1021 TAATATTTGC TTATACAATC TTTCCTGTTT TTTGGGGCTT TTCTGATTAT CACCGGGTGG 1081 TACGAGCTCG ATTCTTGTGA ATGTTGTGTC AGTAGGATGT GAAGTTCCTC AGGCCTCCCC 1141 AGCAGGGCAG AAGTTATATG GCCCA // |
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Sequencing and PCR primers for primer information.
Original, user report and related articles
2019.11.02
GNP_filter3_RDBDEP_html_190927.pl