Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

S-HA-pRc/CMV

Expressing HA-tagged fusion protein, CMV promoter.

Catalog number RDB02136
Resource name S-HA-pRc/CMV
Alternative name S-HA, single HA-tagged expression vector
Clone info. Expression vector. Expressing HA-tagged fusion protein under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor|Developer Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (DNA Cell Biol., 16, 893-896 (1997))。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB02136 S-HA-pRc/CMV DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB02136z.seq
            Remarks, protocol and/or map (gif) RDB02136.gif
            Remarks, protocol and/or map (pdf) RDB02136.pdf

            Original reference

            original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Empty Backbone (English text)
            Featured content Empty Backbone (Japanese text)
            Featured content pCMV_tag and pRSV_tag vector (English text)
            Featured content pCMV_tag and pRSV_tag vector (Japanese text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13988_A6Cmp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: CMV_Forward
            Region: CMV pro,T7 pro,HA,SP6 pro,bGH pA,M13 ori
            Sequence file: RDB13988_A6Cma.seq check
            >02136_13988_A6Cm_2_CMV-Forward_A01_01.ab1
                1 NNNNNNNNNN CNNTTGNNGC NNTGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
               61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
              121 CACTATAGGG AGACCCAAGC TTCCATGGCC TACCCCTACG ACGTGCCCGA CTACGCCGCG
              181 GCCGCTCGAG CATGCATCTA GAGGGCCCTA TTCTATAGTG TCACCTAAAT GCTAGAGCTC
              241 GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG
              301 TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA
              361 TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA
              421 GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG
              481 CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CGAGGGGGGA TCCCCACGCG CCCTGTAGCG
              541 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG
              601 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC
              661 CCCGTCAAGC TCTAAATCGG GNGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
              721 TCGACCCCNA AAAACTTGAN TANGGTGANG GNTCACGTAG TGGGNCATCG CCCNGATAGA
              781 NGGNTTTTCG CCCTTTGACG TNNGANTNNN NNTTCTTNNN TANTGNNNTC NNGNNNNNAN
              841 NTNNNNCNNC ACTCNNCCCT ATNNNNGNNN ANTCNTTTGA NTNATNNNGN ANTTNNNNNN
            901 NTNNNNNNNN NN
            //
            Primer: SV40pro_F_V2
            Region: NeoR
            Sequence file: RDB13988_A6Cmb.seq check
            >02136_13988_A6Cm_2_SV40pro_F_V2_B01_04.ab1
                1 NNNNNNNNNN NNNNNNNNNN CNNNGCTTTT GCNNNNNNTC CCGGGNNCTT GGATATCCNT
               61 TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT
              121 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA
              181 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT
              241 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT
              301 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC
              361 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT
              421 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA
              481 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG
              541 GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC
              601 AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC
              661 CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT
              721 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA
              781 TATTGCTGAA GAGCTTGGCG GCGAATGGNC TGACCGCTTC CTCGTGCTTT ACGGTATCGC
              841 CGCTCCCGAT TCNCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGANTNCT TCTGNNCGGG
              901 ANTCTGGGGG NTCGAAATGA CCGANNNAGC GACNCCCAAC CTGNCNTCAC NANANTTCGA
              961 TNNCNNCNGN CGCCNTCTAT GAAANNNNGG GCNNNNGNNN NNTTTTNNNG GNNNNNNNNN
            1021 N
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl