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S-HA-pRc/CMV

Expressing HA-tagged fusion protein, CMV promoter.

Catalog number RDB02136
Resource name S-HA-pRc/CMV
Alternative name S-HA, single HA-tagged expression vector
Clone info. Expression vector. Expressing HA-tagged fusion protein under the control of CMV promoter.
Vector backbone pRc/CMV (Plasmid)
Size of vector backbone 5.5 kb
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Depositor Takemoto, Yoshihiro | Hashimoto, Yasuhiro | Furuta, Masaaki | Sato, Mitsuru |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (DNA Cell Biol., 16, 893-896 (1997)).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02136 S-HA-pRc/CMV DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB02136z.seq from Depositor
Electronic file Map RDB02136.gif from Depositor
Electronic file PDF RDB02136.pdf from Depositor

Featured content

Featured content pCMV_tag and pRSV_tag vector (English text)
Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)
Featured content pCMV_tag and pRSV_tag vector (Japanese text)

References

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13988_A6Cmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB13988_A6Cma.seq
>02136_13988_A6Cm_2_CMV-Forward_A01_01.ab1
    1 CCTCACTCGC CCATTGACGC AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC
   61 AGAGCTCTCT GGCTAACTAG AGAACCCACT GCTTACTGGC TTATCGAAAT TAATACGACT
  121 CACTATAGGG AGACCCAAGC TTCCATGGCC TACCCCTACG ACGTGCCCGA CTACGCCGCG
  181 GCCGCTCGAG CATGCATCTA GAGGGCCCTA TTCTATAGTG TCACCTAAAT GCTAGAGCTC
  241 GCTGATCAGC CTCGACTGTG CCTTCTAGTT GCCAGCCATC TGTTGTTTGC CCCTCCCCCG
  301 TGCCTTCCTT GACCCTGGAA GGTGCCACTC CCACTGTCCT TTCCTAATAA AATGAGGAAA
  361 TTGCATCGCA TTGTCTGAGT AGGTGTCATT CTATTCTGGG GGGTGGGGTG GGGCAGGACA
  421 GCAAGGGGGA GGATTGGGAA GACAATAGCA GGCATGCTGG GGATGCGGTG GGCTCTATGG
  481 CTTCTGAGGC GGAAAGAACC AGCTGGGGCT CGAGGGGGGA TCCCCACGCG CCCTGTAGCG
  541 GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG
  601 CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC
  661 CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC
  721 TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA
  781 CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA
  841 CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA
  901 TTTCGCCTAT TGTTAAAAAT GAGCTGATTA ACAAAAATTT ACGCGATTTA ACAAAATATA
  961 CGCTAACATT AATATTGCTA ATACAATCTC TGTTTTGGCC TTTCTGATAT CACGGGGTGG
 1021 TACGAGCTCG ATTCGTGATG GTTGTCAGTA GGTTGGAGTC CCAAGTTCGA CGCAGTTGCA
1081 GCATGCATCA TTAGTCCAGC AACAGTG
//
Primer: SV40pro_F_V2 RDB13988_A6Cmb.seq
>02136_13988_A6Cm_2_SV40pro_F_V2_B01_04.ab1
    1 GTCCGACCGT TTTTTGGAGG CTAGGCTTTT GCAAAAGCTC CCGGGAGCTT GGATATCCAT
   61 TTTCGGATCT GATCAAGAGA CAGGATGAGG ATCGTTTCGC ATGATTGAAC AAGATGGATT
  121 GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT GGGCACAACA
  181 GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC GCCCGGTTCT
  241 TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG CAGCGCGGCT
  301 ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG TCACTGAAGC
  361 GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT CATCTCACCT
  421 TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC ATACGCTTGA
  481 TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG CACGTACTCG
  541 GATGGAAGCC GGTCTTGTCG ATCAGGATGA TCTGGACGAA GAGCATCAGG GGCTCGCGCC
  601 AGCCGAACTG TTCGCCAGGC TCAAGGCGCG CATGCCCGAC GGCGAGGATC TCGTCGTGAC
  661 CCATGGCGAT GCCTGCTTGC CGAATATCAT GGTGGAAAAT GGCCGCTTTT CTGGATTCAT
  721 CGACTGTGGC CGGCTGGGTG TGGCGGACCG CTATCAGGAC ATAGCGTTGG CTACCCGTGA
  781 TATTGCTGAA GAGCTTGGCG GCGAATGGGC TGACCGCTTC CTCGTGCTTT ACGGTATCGC
  841 CGCTCCCGAT TCGCAGCGCA TCGCCTTCTA TCGCCTTCTT GACGAGTTCT TCTGAGCGGG
  901 ACTCTGGGGG TTCGAAATGA CCGACCAAGC GACGCCCAAC CTGCCATCAC GAGATTTCGA
  961 TTCCACCCGC CGCCTTCTAT GAAAGGTTGG GCTTCGGAAT CGTTTTCCGG GACGCCCGGC
 1021 TGGATGATCC CTCCAGCGCG GGGATCTCAT GCTGAGTCTT CGCCCACCCC AACTTGTTAA
 1081 TGCAGCTATA TTGTTACAAA TAGCCAATAG CCATCACTAA AATTGACAGA TAGGCATTTC
1141 CTGCATCAGT GTGATTTGGT C
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Takemoto, Y., Expression plasmid vectors with convenient subcloning sites in lambda gt11 that efficiently produce detectable tagged proteins. DNA Cell Biol., 16, 893-896 (1997). PMID 9260932.

2018.12.11

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