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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pRHOHT2 (#RDB02082)

Human tyrosinase expression, Human tyrosinase cDNA, Rat heme oxygenase gene

Clone info. Human tyrosinase expression, Human tyrosinase cDNA, Rat heme oxygenase gene
Vector backbone pUC8 (Plasmid)
Size of vector backbone 5.7 kb
Selectable markers Amp^r
Gene/insert name Human Tyrosinase+heme oxygenase cDNA Rat Hmox1 cDNA
Reference of insert sequence M27160 (DDBJ accession number)
Depositor|Developer Shibahara, Shigeki |
 
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Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (Biochem. Biophys. Res. Commun., 162, 984-990 (1989). Tohoku J. Exp. Med., 156, 403-414 (1988).).
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必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
提供同意書 (MTA, 営利目的用)[Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (Biochem. Biophys. Res. Commun., 162, 984-990 (1989). Tohoku J. Exp. Med., 156, 403-414 (1988).)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02082 pRHOHT2 DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pRHOHT2 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB02082).

Reference section:

Takeda, A., Tomita, Y., Okinaga, S., Tagami, H., Shibahara, S., Functional analysis of the cDNA encoding human tyrosinase precursor. Biochem. Biophys. Res. Commun., 162, 984-990 (1989). PMID 2504160.
Shibahara, S., Tomita, Y., Tagami, H., Müller, R.M., Cohen, T., Molecular basis for the heterogeneity of human tyrosinase. Tohoku J. Exp. Med., 156, 403-414 (1988). PMID 2854305. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12745_A4Cr.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer:
Sequence file: RDB12745_A4Cra.seq check
>02082_12745_A4Cr_M13(-40)_A07_01.ab1
    1 GCGGCCAGTT CCCAGCTTCG TCCTCCAGCT CACATTCCCC GCCTGTAAGC ACTGGAGCCA
   61 TCTATCCCCC ACTGTGCACA CATGGGGATG AACCTTGGAA AAGTCAGTCT AGGCAGGTGT
  121 TAGTAAAACA AACAGGACTA TGTACATAAA GGATTGAGAA AACCAGTCAT CCTTAGAGTT
  181 CCGGGAAGGT AGACTCAAGT ATGTAGACTT GGGGCCCGGA TGGGTCAATC CCTGGGTTTG
  241 CCCACCAGCA TCTCACAGCT AGAATTGTCT CCTAGTTCTG GAACCTTCCA GATTCCTGAG
  301 AGGCTGGCTA CTCCTGCCTT CCTATCACTG AATAGAGTAG AGGGTTCTGG AAAGGACCCA
  361 AAAAATTCTG CTAAGAGGCA ATTCACTCAG ATTTCCTTTT TTTTTTTTTA AGAGCTTGCC
  421 AGAGTCATAT GATTTATCCC CATACAGGCC AGGCCGACCA GCCTCGGGGC GGGGTGTTGC
  481 AACAACAGCC AGAACGCCGG CTCAGCTGGG CGGCCACCAC GTGACTCGAG TACTTAAAGG
  541 GCTGGCGCGG GCAGCTGCTC GCTCACAGTC GCCAGTCGCC TCCAGAGTTT CCGCCTCCAA
  601 CCAGCGAGTG GAGCCTGCCG GAGCAGGGTC GACCGACCTT GTGAGGACTA GAGGAAGAAT
  661 GCTCCTGGCT GTTTTGTACT GCCTGCTGTG GAGTTTCCAG ACCTCCGCTG GCCATTTCCC
  721 TAGAGCCTGT GTCTCCTCTA AGAACCTGAT GGAGAAGGAA TGCTGTCCAC CGTGGAGCGG
  781 GGACAGGAGT CCCTGTGGCC AGCTTTCAGG CAGAGGTTCC TGTCAGAATA TCCTTCTGTC
  841 CAATGCACCA CTTGGGCCTC AATTTCCCTT CACAGGGGTG GATGACCGGG AGTCGTGGCC
  901 TTCCGTCTTT TATAATAGGA CCTGCCAGTG CTCTGGCAAC TTCATGGGGA TTCAACTGTG
  961 GAAACTGCAA GTTTGGCTTT TGGGGGACCA AAACTGCACA GAGAGACGAC TCTTGGTGAG
 1021 AAGAAAACAT CTTTCGATTT GAGTGCCCCA GAGAGACAAT TTTTGCTACC TCACTTTAAG
 1081 CAAGCCATAC CATCAGCTCA GACTATGTCA TCCATAGACT ATGGCTAATG AAAATGGAAT
1141 TCAACAC
//
Primer:
Sequence file: RDB12745_A4Crb.seq check
>02082_12745_A4Cr_Reverse2_B07_04.ab1
    1 AGGTAACCTT ATTAACGAAA TTCAAAATTG TTTCTCTTCT CTCCCTTTTC TTCCCTTCAC
   61 TTGCCCACCC TTTAGGCACC AGCGTTTGAA CCTGGGACAT CCCATATGTT AGGCAAGCAT
  121 TCTACCACTG GGCTATATAT ACCTAGCATT TTTGCTTACT TAATTGTATA TGTATAGGGG
  181 TTGGGGATTT AGCTCAGTGG TAGAGCGCTT GCCTAGCAAG CGCAAGGCCC TGGGTTTGGT
  241 CCCCAGCTCC GAAAAAAAAA ATTGTATATG TATAGATATT TTGCCTGCAC ACGTGTCAGT
  301 GCATTGCATG TGCACCTGGT GCCAAAGAGG ACAGAAAAAA AAATCATTAG ATCCCCTGGA
  361 ATTAGAGTTA AACCGTTATG AGCCACCATT TGGGTGCTGG GAATCAACCC AGGGTCTTCT
  421 GGAAGAGCAG GCCGTGCTTT GAAGCACTGA GCCATCTCTT TAGCGCCCCT CCCCCAACTT
  481 GCCTTCCTTT ATCACAATAC ATATTTTCAC AGATGAGGAA ACTAAGCTCA GGATAGGAGA
  541 GCTAGCTCAG GGTAAGAGAG CCATCTTTGC TATCTGACAT CAATGTTTTG CATATATCTA
  601 TACCCCGATC CTTAATTTCA CTTTATCCTT AATTTCATAA AGAATGGCTT CTGGCCAGGT
  661 GGTGACGTAA AGACAAGCAT ATCTCCATGA GTTTGAGACC AGCCTGGTCT ATGGAGCAAG
  721 TTCTACAGCC AAGACTTCAA TGAAACCCTG TCTTGAAAAA ACAAAATGAC AACAAGAATG
  781 ACTTCTGGAC CTGGCTGTAT GTATATGAGT CTTCCAGAAT GGCCTTTCTT GTGTGGTGAC
  841 TCTGGGACAC CACCTCAGAG CTCTTTAGCT ATGTGCCTCC TTGGAGTTCT GGAGTTTCTG
  901 TGCACAGACA TCACCGGATT CCCTCCTCAG CTCTGAGAGA CAGATGTATT GCTCATCTTT
  961 CCAGGGAGGA AAATGAGATT TGAAGCACTG TGCCCAAGAC TTGGCCAATA GAGGCCAGTA
 1021 TGGGGGGACA AAGACCCTTC TGACCCCCCA GTCCTTCTAG CACTAGTGGG GACATAGTGG
 1081 TACCCCATCC CCGTTCGGCC TTCACTGGAC GTTTTCAACT TCCAGATGGG AAGCTGTTAT
1141 TGTTCCTACT GTTTTTGCGT G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Takeda, A., Functional analysis of the cDNA encoding human tyrosinase precursor. Biochem. Biophys. Res. Commun., 162, 984-990 (1989). PMID 2504160.
original Shibahara, S., Molecular basis for the heterogeneity of human tyrosinase. Tohoku J. Exp. Med., 156, 403-414 (1988). PMID 2854305. [link to RRC of NBRP]
user_report Takekoshi, S., Stimulation of melanogenesis by nordihydroguaiaretic Acid in human melanoma cells. Acta Histochem. Cytochem. 47 (5): 203-210 (2014). PMID 25861126. [link to RRC of NBRP]