Resource data sheet
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pAxCAhFL

Shuttle vector to generate rAd expressing human FAS Ligand

Catalog number RDB02019
Resource name pAxCAhFL
Clone info. Shuttle vector to generate recombinant adenovirus Human Fas Ligand expression. cDNA fragment 195 to 1126nt of NM_000639.2 (CDS, 195 to 1040nt) is cloned.
Use the COS-TPC method for production of adenovirus. Known difference: C to T substitution at 756nt of NM_000639.2 (Leu, synonym).
Vector backbone pAxcw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 42.5 kb
Selectable markers Amp^r
Gene/insert name Human Fas ligand (FasL) cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human FASLG (NCBI Gene 356) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature designated by the DEPOSITOR (Human Gene Therapy, 9, 2683-2689 (1998).) and an acknowledgment to the DEPOSITOR are requested.
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Remarks Remember that you will be working with samples containing infectious virus.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB02019 pAxCAhFL DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB02019.gif from Depositor

Original reference

original Shinoura, N., Construction, propagation, and titer estimation of recombinant adenoviruses carrying proapoptotic genes. Human Gene Therapy, 9, 2683-2689 (1998). PMID 9874266.

Further references such as user reports and related articles (go to bottom)

Featured content


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB15036_A7Egp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 | Sequence: RDB15036_A7Ega.seq
>02019_15036_A7Eg_pAxCA_F1_F05_17_ABI24.ab1
    1 TCAAACCATG GTTTTCCTTG GCCTTTCTTT CTTTTTCCTA CAGCTCCTGG GCAACGTGCT
   61 GGTTATTGTG CTGTCTCATC ATTTTGGCAA AGAATTCGAT AAGCTTGCAT GCAGCAGCCC
  121 TTCAATTACC CATATCCCCA GATCTACTGG GTGGACAGCA GTGCCAGCTC TCCCTGGGCC
  181 CCTCCAGGCA CAGTTCTTCC CTGTCCAACC TCTGTGCCCA GAAGGCCTGG TCAAAGGAGG
  241 CCACCACCAC CACCGCCACC GCCACCACTA CCACCTCCGC CGCCGCCGCC ACCACTGCCT
  301 CCACTACCGC TGCCACCCCT GAAGAAGAGA GGGAACCACA GCACAGGCCT GTGTCTCCTT
  361 GTGATGTTTT TCATGGTTCT GGTTGCCTTG GTAGGATTGG GCCTGGGGAT GTTTCAGCTC
  421 TTCCACCTAC AGAAGGAGCT GGCAGAACTC CGAGAGTCTA CCAGCCAGAT GCACACAGCA
  481 TCATCTTTGG AGAAGCAAAT AGGCCACCCC AGTCCACCCC CTGAAAAAAA GGAGCTGAGG
  541 AAAGTGGCCC ATTTAACAGG CAAGTCCAAC TCAAGGTCCA TGCCTCTGGA ATGGGAAGAC
  601 ACCTATGGAA TTGTCCTGCT TTCTGGAGTG AAGTATAAGA AGGGTGGCCT TGTGATCAAT
  661 GAAACTGGGT TGTACTTTGT ATATTCCAAA GTATACTTCC GGGGTCAATC TTGCAACAAC
  721 CTGCCCCTGA GCCACAAGGT CTACATGAGG AACTCTAAGT ATCCCCAGGA TCTGGTGATG
  781 ATGGAGGGGA AGATGATGAG CTACTGCACT ACTGGGCAGA TGTGGGCCCG CAGCAGCTAC
  841 CTGGGGGGCA GTGTTCAATC TTACCAGTGC TGATCATTTA TATGTCAACG TATCTGAGCT
  901 CTCTCTGGTC AATTTTGAGG AATCTCAGAC GTTTTTCGGC TTATATAAGC TCTAAGAGAA
  961 GCACTTTGGA TTCTTTCCAT TATGATTCTT TGTTACAGGC ACCGAGAATG TTGTATTCGG
 1021 TGAGGTCTTC TTACATGCAG CCCGGGGGAT CCACTAGTTC TAGAATTCAC TCTCAAGGTG
 1081 CAGCCTGCCT ATTCAGAAGT GATGCTGGTG GTGCCCAAAT GCCTGGCTTC ACATACACTT
1141 GAGATTCTTT TTCCCCTCTT GCCAAAAATT ATGGGGAACA TCTATGTGA
//
Primer: pAxCA_R1 | Sequence: RDB15036_A7Egb.seq
>02019_15036_A7Eg_1_pAxCA_R1_F07_16_ABI24.ab1
    1 CAAGTCAACA AACGAACCCT TTCTAGATAA AGCAGCCTGC ACCTGAGGAG TGAATTCTAG
   61 AACTAGTGGA TCCCCCGGGC TGCATGTAAG AAGACCCTCA CCGAATACAA CATTCTCGGT
  121 GCCTGTAACA AAGAATCATA ATGGAAAGAA TCCCAAAGTG CTTCTCTTAG AGCTTATATA
  181 AGCCGAAAAA CGTCTGAGAT TCCTCAAAAT TGACCAGAGA GAGCTCAGAT ACGTTGACAT
  241 ATAAATGATC AGCACTGGTA AGATTGAACA CTGCCCCCAG GTAGCTGCTG CGGGCCCACA
  301 TCTGCCCAGT AGTGCAGTAG CTCATCATCT TCCCCTCCAT CATCACCAGA TCCTGGGGAT
  361 ACTTAGAGTT CCTCATGTAG ACCTTGTGGC TCAGGGGCAG GTTGTTGCAA GATTGACCCC
  421 GGAAGTATAC TTTGGAATAT ACAAAGTACA ACCCAGTTTC ATTGATCACA AGGCCACCCT
  481 TCTTATACTT CACTCCAGAA AGCAGGACAA TTCCATAGGT GTCTTCCCAT TCCAGAGGCA
  541 TGGACCTTGA GTTGGACTTG CCTGTTAAAT GGGCCACTTT CCTCAGCTCC TTTTTTTCAG
  601 GGGGTGGACT GGGGTGGCCT ATTTGCTTCT CCAAAGATGA TGCTGTGTGC ATCTGGCTGG
  661 TAGACTCTCG GAGTTCTGCC AGCTCCTTCT GTAGGTGGAA GAGCTGAAAC ATCCCCAGGC
  721 CCAATCCTAC CAAGGCAACC AGAACCATGA AAAACATCAC AAGGAGACAC AGGCCTGTGC
  781 TGTGGTTCCC TCTCTTCTTC AGGGGTGGCA GCGGTAGTGG AGGCAGTGGT GGCGGCGGCG
841 GCGGAGGTGG TAGTGGTGGC GGTGGCGGTG GTGGTGGTGG CC
//
Primer: pAxcwit2-Fw | Sequence: RDB15036_A7Egc.seq
>02019_15036_A7Eg_1_pAxcwit2_Fw_D07_10_ABI24.ab1
    1 GCCCGCAGTG CCCTCCCCAC CACGTGGTCG GGGCCTCAGC TCCTCGGTCA CATCCAGCAT
   61 CACAGGCTGG TTCCTAATAT GTTTACCGCC ACACTCGCAG GGTCTGCACC TGGTGCGGGT
  121 CTCATCGTAC CTCAGCACCT TCCAGATCCA TCGTCGCGAG TCGACAATCG ATGTCGACAT
  181 TGATTATTGA CTAGTTATTA ATAGTAATCA ATTACGGGGT CATTAGTTCA TAGCCCATAT
  241 ATGGAGTTCC GCGTTACATA ACTTACGGTA AATGGCCCGC CTGGCTGACC GCCCAACGAC
  301 CCCCGCCCAT TGACGTCAAT AATGACGTAT GTTCCCATAG TAACGCCAAT AGGGACTTTC
  361 CATTGACGTC AATGGGTGGA GTATTTACGG TAAACTGCCC ACTTGGCAGT ACATCAAGTG
  421 TATCATATGC CAAGTACGCC CCCTATTGAC GTCAATGACG GTAAATGGCC CGCCTGGCAT
  481 TATGCCCAGT ACATGACCTT ATGGGACTTT CCTACTTGGC AGTACATCTA CGTATTAGTC
  541 ATCGCTATTA CCATGGTCGA GGTGAGCCCC ACGTTCTGCT TCACTCTCCC CATCTCCCCC
  601 CCCTCCCCAC CCCCAATTTT GTATTTATTT ATTTTTTAAT TATTTTGTGC AGCGATGGGG
661 GCGGGGGGGG GGGGGGGGGG
//
Primer: Amp_R | Sequence: RDB15036_A7Egd.seq
>02019_15036_A7Eg_1_Amp_R_E07_13_ABI24.ab1
    1 TAGGGGGAAC CAGGCGTGAG GAGGCTTGTA TCAGGGTTAT TGTCTCATGA GCGGATACAT
   61 ATTTGAATGT ATCCTATTAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA
  121 GTGCCACCTG ACGTCTAAGA AACCATTATT ATCATGACAT TAACCTATAA AAATAGGCGT
  181 ATCACGAGGC CCTTTCGTCT TCAAGAATTC CGGATCCGGG CCAATATGAT AATGAGGGGG
  241 TGGAGTTTGT GACGTGGCGC GGGGCGTGGG AACGGGGCGG GTGACGTAGT AGTGTGGCGG
  301 AAGTGTGATG TTGCAAGTGT GGCGGAACAC ATGTAAGCGA CGGATGTGGC AAAAGTGACG
  361 TTTTTGGTGT GCGCCGGTGT ACACAGGAAG TGACAATTTT CGCGCGGTTT TAGGCGGATG
  421 TTGTAGTAAA TTTGGGCGTA ACCGAGTAAG ATTTGGCCAT TTTCGCGGGA AAACTGAATA
  481 AGAGGAAGTG AAATCTGAAT AATTTTGTGT TACTCATAGC GCGTAATATT TGTCTAGGGC
  541 CGCGGGGACT TTGACCGTTT ACGTGGAGAC TCGCCCAGGT GTTTTTCTCA GGTGTTTTCC
  601 GCGTTCCGGG TCAAAGTTGG CGTTTTATTA TTATAGTCAG CATCGATGAG CTTGATAGCT
  661 TGGGCTGCAG GTCGAGGGAT CTCCATAAGA GAAGAGGGAC AGCTATGACT GGGAGTAGTC
  721 AGGAGAGGAG GAAAAATCTG GCTAGTAAAA CATGTAAGGA AAATTTTAGG GATGTTAAAG
  781 AAAAAAATAA CACAAAACAA AATATAAAAA AAATCTAACC TCAAGTCAAG GCTTTTCTAT
  841 GGAATAAGGA ATGGACAGCA GGGGGCTGTT TCATATACTG ATGACCTCTT TATAGCCAAC
  901 CCTTTGTTCA TGGCAGCCAG CATATGGGCA TATGTTGCCC AAACTCTAAA CCAAATACTC
  961 ATTCTGATGT TTTAATGATT TGCCCTCCCA TATGTCCTTC CGAGTGAGAG ACAACAAAAA
 1021 ATTCCCAACA CACTAATTGC ATGAAAATAA AATTTCCCTT TTATTAGGCC AGAAGTCAGA
 1081 TGGCTTCAAG GGGGCTTCAT GATGTCCCCA TATTTTTTGG GCCAGAAGGG AAAGGATCCT
1141 CAATTGTATT TGGTGGAGCA AGGGACAATT GGCCCACACA CGCAATCCAA CCATTGC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Shinoura, N., Construction, propagation, and titer estimation of recombinant adenoviruses carrying proapoptotic genes. Human Gene Therapy, 9, 2683-2689 (1998). PMID 9874266.

2019.09.18

GNP_filter3_RDBDEP_html_190816.pl