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pME18S-Lyn

Expression vector of human lyn cDNA (partial)

Catalog number RDB01966
Resource name pME18S-Lyn
Alternative name Lyn cDNA (264-1949)
Clone info. Expression vector of human lyn cDNA (partial). MluI fragment of lyn cDNA (264-1949 nt.) was inserted into EcoRI site of pME18S after deletion of stuffer sequence of pME18S (Mol. Cell. Biol. 8, 466-472).
Vector backbone pME18S (Plasmid)
Size of vector backbone 3,411 bp
Selectable markers Amp^r
Gene/insert name Human lyn (tyrosine Kinase) cDNA
Depositor Yamamoto, Tadashi |
 
Other clones in our bank human LYN (NCBI Gene 4067) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biol. Chem., 268, 27413-27419 (1993).).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01966 pME18S-Lyn DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01966.gif from Depositor

Original reference

original Takeuchi, M., Functional and physical interaction of protein-tyrosine kinases Fyn and Csk in the T-cell signaling system. J. Biol. Chem., 268, 27413-27419 (1993). PMID 8262983.
original Yamanashi, Y., The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Mol. Cell. Biol., 7, 237-243 (1987). PMID 3561390.

Further references such as user reports and related articles (go to bottom)

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Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB01966_A9Hqp1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Region: SRa pro,insert5' | Primer: SV40pro_ori_F | Sequence: RDB01966_A9Hqa.seq
>01966_01966_A9Hq_1_SV40pro_ori_F_A03_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCGCC NCNGCCTCTG NNCTATTCNN GAAGTAGTGA GGANGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC TCGATCGAGG GGCTCGCATC TCTCCTTCAC
  121 GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC
  181 CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG CTCAGGTCGA
  241 GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG CTCTCCACGC
  301 TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT
  361 ACAGATCCAA GCTCTGAAAA ACCAGAAAGT TAACTGGTAA GTTTAGTCTT TTTGTCTTTT
  421 ATTTCAGGTC CCGGATCCGG TGGTGGTGCA AATCAAAGAA CTGCTCCTCA GTGGATGTTG
  481 CCTTTACTTC TAGGCCTGTA CGGAAGTGTT ACTTCTGCTC TAAAAGCTGG ACGCGTCCCG
  541 CCGCCCCGAA ACTTTCACCG CGAGCGGGAA ATATGGGATG TATAAAATCA AAAGGGAAAG
  601 ACAGCTTGAG TGACGATGGA GTAGATTTGA AGACTCAACC AGTACGTAAT ACTGAAAGAA
  661 CTATTTATGT GAGAGATCCA ACGTCCAATA AACAGCAAAG GCCAGTTCCA GAATCTCAGC
  721 TTTTACCTGG ACAGANGTTT CAAACTAAAG ATCCAGANGA ACAAGGAGAC ATTGTGGTAG
  781 CCTTGTACCC CTATGATGGC ATCCACCCGG ACGANTTGTC TTTCAAGAAA NGANANAAGA
841 NGAAAGTCCT GNN
//
Region: SV40 poly(A),insert3' | Primer: p15Aori_F | Sequence: RDB01966_A9Hqb.seq
>01966_01966_A9Hq_1_p15Aori_F_A05_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCG TNNGGNGGGC GGNNCCTATG GAAAAACGCC
   61 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT
  121 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC
  181 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGGAA
  241 TTAATTCGAG CTCGGTACCC GGGGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC
  301 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG
  361 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT
  421 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTC TCTAGACTAG TCTAGCGGCC
  481 GCGACCTGCA GGCGCAGAAC TGGTAGGTAT GGAAGATCCC TCGAGACGCG TCGATCCAGG
  541 CACGGCAGAT GATCCCCGCA CATGAAATCA TAAGTGCAAC CAGTGATGGT TACTTTTCCT
  601 CTCTAAATGA GGCAGCCACC CCTGCCAAAT GGACGGGTCT CCCTGTGCTC TAAGGCTGCT
  661 GCTGGTATTG CCCTTCCGTG GCTGTGTAGA AATCATCCAG GACGCTCTGT AAGTAGTCAA
  721 ACGTTGGTCT CTCTTCTGCC TTTTCTTTCC AGCACATTTT CATAATGTCA TANAGCTCAT
  781 CTGGGCAGTT CTCCACACGG GGCATCCTGT AGCCCTGGGN CAGGGCGGTC ATCACGTCNG
841 CATTAGTTCT CCCTGGGT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Takeuchi, M., Functional and physical interaction of protein-tyrosine kinases Fyn and Csk in the T-cell signaling system. J. Biol. Chem., 268, 27413-27419 (1993). PMID 8262983.
original Yamanashi, Y., The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Mol. Cell. Biol., 7, 237-243 (1987). PMID 3561390.
reference Takebe, Y., SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol., 8, 466-472 (1988). PMID 2827008.

2019.09.18

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