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pME18S-Lyn

Expression vector of human lyn cDNA (partial)

Catalog number RDB01966
Resource name pME18S-Lyn
Alternative name Lyn cDNA (264-1949)
Clone info. Expression vector of human lyn cDNA (partial). MluI fragment of lyn cDNA (264-1949 nt.) was inserted into EcoRI site of pME18S after deletion of stuffer sequence of pME18S (Mol. Cell. Biol. 8, 466-472).
Vector backbone pME18S (Plasmid)
Size of vector backbone 3,411 bp
Selectable markers Amp^r
Gene/insert name Human lyn (tyrosine Kinase) cDNA
Depositor|Developer Yamamoto, Tadashi |
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External Database
Human lyn (tyrosine Kinase)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biol. Chem., 268, 27413-27419 (1993).).
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem., 268, 27413-27419 (1993))。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01966 pME18S-Lyn DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01966.gif

          Original reference

          original Takeuchi, M., Functional and physical interaction of protein-tyrosine kinases Fyn and Csk in the T-cell signaling system. J. Biol. Chem., 268, 27413-27419 (1993). PMID 8262983.
          original Yamanashi, Y., The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Mol. Cell. Biol., 7, 237-243 (1987). PMID 3561390.

          Further references such as user reports and related articles (go to bottom)

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          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB01966_A9Hqp1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pro_ori_F
          Region: SRa pro,insert5'
          Sequence file: RDB01966_A9Hqa.seq check
          >01966_01966_A9Hq_1_SV40pro_ori_F_A03_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNCGCC NCNGCCTCTG NNCTATTCNN GAAGTAGTGA GGANGCTTTT
             61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC TCGATCGAGG GGCTCGCATC TCTCCTTCAC
            121 GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC
            181 CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG CTCAGGTCGA
            241 GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG CTCTCCACGC
            301 TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT
            361 ACAGATCCAA GCTCTGAAAA ACCAGAAAGT TAACTGGTAA GTTTAGTCTT TTTGTCTTTT
            421 ATTTCAGGTC CCGGATCCGG TGGTGGTGCA AATCAAAGAA CTGCTCCTCA GTGGATGTTG
            481 CCTTTACTTC TAGGCCTGTA CGGAAGTGTT ACTTCTGCTC TAAAAGCTGG ACGCGTCCCG
            541 CCGCCCCGAA ACTTTCACCG CGAGCGGGAA ATATGGGATG TATAAAATCA AAAGGGAAAG
            601 ACAGCTTGAG TGACGATGGA GTAGATTTGA AGACTCAACC AGTACGTAAT ACTGAAAGAA
            661 CTATTTATGT GAGAGATCCA ACGTCCAATA AACAGCAAAG GCCAGTTCCA GAATCTCAGC
            721 TTTTACCTGG ACAGANGTTT CAAACTAAAG ATCCAGANGA ACAAGGAGAC ATTGTGGTAG
            781 CCTTGTACCC CTATGATGGC ATCCACCCGG ACGANTTGTC TTTCAAGAAA NGANANAAGA
          841 NGAAAGTCCT GNN
          //
          Primer: p15Aori_F
          Region: SV40 poly(A),insert3'
          Sequence file: RDB01966_A9Hqb.seq check
          >01966_01966_A9Hq_1_p15Aori_F_A05_01_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCG TNNGGNGGGC GGNNCCTATG GAAAAACGCC
             61 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT
            121 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC
            181 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGGAA
            241 TTAATTCGAG CTCGGTACCC GGGGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC
            301 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG
            361 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT
            421 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTC TCTAGACTAG TCTAGCGGCC
            481 GCGACCTGCA GGCGCAGAAC TGGTAGGTAT GGAAGATCCC TCGAGACGCG TCGATCCAGG
            541 CACGGCAGAT GATCCCCGCA CATGAAATCA TAAGTGCAAC CAGTGATGGT TACTTTTCCT
            601 CTCTAAATGA GGCAGCCACC CCTGCCAAAT GGACGGGTCT CCCTGTGCTC TAAGGCTGCT
            661 GCTGGTATTG CCCTTCCGTG GCTGTGTAGA AATCATCCAG GACGCTCTGT AAGTAGTCAA
            721 ACGTTGGTCT CTCTTCTGCC TTTTCTTTCC AGCACATTTT CATAATGTCA TANAGCTCAT
            781 CTGGGCAGTT CTCCACACGG GGCATCCTGT AGCCCTGGGN CAGGGCGGTC ATCACGTCNG
          841 CATTAGTTCT CCCTGGGT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Takeuchi, M., Functional and physical interaction of protein-tyrosine kinases Fyn and Csk in the T-cell signaling system. J. Biol. Chem., 268, 27413-27419 (1993). PMID 8262983.
          original Yamanashi, Y., The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Mol. Cell. Biol., 7, 237-243 (1987). PMID 3561390.
          reference Takebe, Y., SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol., 8, 466-472 (1988). PMID 2827008.

          2022.02.09

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