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pME18S-Lyn (#RDB01966)

Expression vector of human lyn cDNA (partial)

Alternative name Lyn cDNA (264-1949)
Clone info. Expression vector of human lyn cDNA (partial). MluI fragment of lyn cDNA (264-1949 nt.) was inserted into EcoRI site of pME18S after deletion of stuffer sequence of pME18S (Mol. Cell. Biol. 8, 466-472).
Vector backbone pME18S (Plasmid)
Size of vector backbone 3,411 bp
Selectable markers Amp^r
Gene/insert name Human lyn (tyrosine Kinase) cDNA
Depositor|Developer Yamamoto, Tadashi |
 
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biol. Chem., 268, 27413-27419 (1993).).
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biol. Chem., 268, 27413-27419 (1993))。

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RDB01966 pME18S-Lyn DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pME18S-Lyn was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01966).

Reference section:

Takeuchi, M., Kuramochi, S., Fusaki, N., Nada, S., Kawamura-Tsuzuku, J., Matsuda, S., Semba, K., Toyoshima, K., Okada, M., Yamamoto, T., Functional and physical interaction of protein-tyrosine kinases Fyn and Csk in the T-cell signaling system. J. Biol. Chem., 268, 27413-27419 (1993). PMID 8262983.
Yamanashi, Y., Fukushige, S., Semba, K., Sukegawa, J., Miyajima, N., Matsubara, K., Yamamoto, T., Toyoshima, K., The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Mol. Cell. Biol., 7, 237-243 (1987). PMID 3561390. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB01966_A9Hqp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: SV40pro_ori_F ()
Region: SRa pro,insert5'
Sequence file: RDB01966_A9Hqa.seq check
>01966_01966_A9Hq_1_SV40pro_ori_F_A03_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNCGCC NCNGCCTCTG NNCTATTCNN GAAGTAGTGA GGANGCTTTT
   61 TTGGAGGCCT AGGCTTTTGC AAAAAGCTCC TCGATCGAGG GGCTCGCATC TCTCCTTCAC
  121 GCGCCCGCCG CCCTACCTGA GGCCGCCATC CACGCCGGTT GAGTCGCGTT CTGCCGCCTC
  181 CCGCCTGTGG TGCCTCCTGA ACTGCGTCCG CCGTCTAGGT AAGTTTAAAG CTCAGGTCGA
  241 GACCGGGCCT TTGTCCGGCG CTCCCTTGGA GCCTACCTAG ACTCAGCCGG CTCTCCACGC
  301 TTTGCCTGAC CCTGCTTGCT CAACTCTACG TCTTTGTTTC GTTTTCTGTT CTGCGCCGTT
  361 ACAGATCCAA GCTCTGAAAA ACCAGAAAGT TAACTGGTAA GTTTAGTCTT TTTGTCTTTT
  421 ATTTCAGGTC CCGGATCCGG TGGTGGTGCA AATCAAAGAA CTGCTCCTCA GTGGATGTTG
  481 CCTTTACTTC TAGGCCTGTA CGGAAGTGTT ACTTCTGCTC TAAAAGCTGG ACGCGTCCCG
  541 CCGCCCCGAA ACTTTCACCG CGAGCGGGAA ATATGGGATG TATAAAATCA AAAGGGAAAG
  601 ACAGCTTGAG TGACGATGGA GTAGATTTGA AGACTCAACC AGTACGTAAT ACTGAAAGAA
  661 CTATTTATGT GAGAGATCCA ACGTCCAATA AACAGCAAAG GCCAGTTCCA GAATCTCAGC
  721 TTTTACCTGG ACAGANGTTT CAAACTAAAG ATCCAGANGA ACAAGGAGAC ATTGTGGTAG
  781 CCTTGTACCC CTATGATGGC ATCCACCCGG ACGANTTGTC TTTCAAGAAA NGANANAAGA
841 NGAAAGTCCT GNN
//
Primer: p15Aori_F ()
Region: SV40 poly(A),insert3'
Sequence file: RDB01966_A9Hqb.seq check
>01966_01966_A9Hq_1_p15Aori_F_A05_01_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNTCG TNNGGNGGGC GGNNCCTATG GAAAAACGCC
   61 AGCAACGCGG CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CATGTTCTTT
  121 CCTGCGTTAT CCCCTGATTC TGTGGATAAC CGTATTACCG CCTTTGAGTG AGCTGATACC
  181 GCTCGCCGCA GCCGAACGAC CGAGCGCAGC GAGTCAGTGA GCGAGGAAGC GGAAGAGGAA
  241 TTAATTCGAG CTCGGTACCC GGGGATCCAG ACATGATAAG ATACATTGAT GAGTTTGGAC
  301 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG
  361 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT
  421 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTC TCTAGACTAG TCTAGCGGCC
  481 GCGACCTGCA GGCGCAGAAC TGGTAGGTAT GGAAGATCCC TCGAGACGCG TCGATCCAGG
  541 CACGGCAGAT GATCCCCGCA CATGAAATCA TAAGTGCAAC CAGTGATGGT TACTTTTCCT
  601 CTCTAAATGA GGCAGCCACC CCTGCCAAAT GGACGGGTCT CCCTGTGCTC TAAGGCTGCT
  661 GCTGGTATTG CCCTTCCGTG GCTGTGTAGA AATCATCCAG GACGCTCTGT AAGTAGTCAA
  721 ACGTTGGTCT CTCTTCTGCC TTTTCTTTCC AGCACATTTT CATAATGTCA TANAGCTCAT
  781 CTGGGCAGTT CTCCACACGG GGCATCCTGT AGCCCTGGGN CAGGGCGGTC ATCACGTCNG
841 CATTAGTTCT CCCTGGGT
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Takeuchi, M., Functional and physical interaction of protein-tyrosine kinases Fyn and Csk in the T-cell signaling system. J. Biol. Chem., 268, 27413-27419 (1993). PMID 8262983.
original Yamanashi, Y., The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Mol. Cell. Biol., 7, 237-243 (1987). PMID 3561390. [link to RRC of NBRP]
reference Takebe, Y., SR alpha promoter: an efficient and versatile mammalian cDNA expression system composed of the simian virus 40 early promoter and the R-U5 segment of human T-cell leukemia virus type 1 long terminal repeat. Mol. Cell. Biol., 8, 466-472 (1988). PMID 2827008.