Please review the QC test results indicated by icon below as well as clone information before placing your order.

pGMT20 (#RDB01960)

E. coli - S. cerevisiae shuttle vector

Alternative name	E. coli-S. cerevisiae shuttle vector
Clone info.	E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, TRP1, GAL1-10 promoter, Ampicillin
Vector backbone	pGMT20 (Plasmid)
Size of vector backbone	5,432 bp
Selectable markers	Amp^r (E. coli), TRP1 (S. cerevisiae)
Depositor|Developer	Iha, Hidekatsu |

 

Sequence	RDB01960z.seq
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Distribution information

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us.
Terms and conditions for distribution	Acknowledgement to Depositor and Developer in publication.

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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01960	pGMT20	DNA solution	Please contact us.

Please review the QC test results indicated by icon below as well as clone information before placing your order.

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How to cite this biological resource

Materials & Methods section:

The pGMT20 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01960).

Reference section:

Iha, H., Tsurugi, K., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

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QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.

Test sheet	RDB13124_A5Bgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev Sequence file: RDB13124_A5Bga.seq
>01960_13124_A5Bg_ADHrev_D11_11.ab1     1 TGGAGTCTCA AGATAAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA    61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCCCGG   121 AATTAGCTTG GCTGCAGGTC GACTCACAGA TCCTCTTCGG AAATCAGTTT CTGTTCAGAA   181 TTCGAGCTCG GTACCCGGGG ATCCTCTAGA GTCAATTCGA TCCGGGGTTT TTTCTCCTTG   241 ACGTTAAAGT ATAGAGGTAT ATTAACAATT TTTTGTTGAT ACTTTTATTA CATTTGAATA   301 AGAAGTAATA CAAACCGAAA ATGTTGAAAG TATTAGTTAA AGTGGTTATG CAGTTTTTGC   361 ATTTATATAT CTGTTAATAG ATCAAAAATC ATCGCTTCGC TGATTAATTA CCCCAGAAAT   421 AAGGCTAAAA AACTAATCGC ATTATCATCC TATGGTTGTT AATTTGATTC GTTCATTTGA   481 AGGTTTGTGG GGCCAGGTTA CTGCCAATTT TTCCTCTTCA TAACCATAAA AGCTAGTATT   541 GTAGAATCTT TATTGTTCGG AGCAGTGCGG CGCGAGGCAC ATCTGCGTTT CAGGAACGCG   601 ACCGGTGAAG ACGAGGACGC ACGGAGGAGA GTCTTCCTTC GGAGGGCTGT CACCCGCTCG   661 GCGGCTTCTA ATCCGTACTT CAATATAGCA ATGAGCAGTT AAGCGTATTA CTGAAAGTTC   721 CAAAGAGAAG GTTTTTTTAG GCTAAGATAA TGGGGCTCTT TACATTTCCA CAACATATAA   781 GTAAGATTAG ATATGGATAT GTATATGGAT ATGTATATGG TGGTAATGCC ATGTAATATG   841 ATTATTAAAC TTCTTTGCGT CCATCCAAAA AAAAAAGTAA GAATTTTTTG AAAATTCAAT   901 ATAAATGACA GCTCAGTTAC AAAGTGAAAG TGACCTGCAG GCATGCAAGC AACGAAGCAT   961 CTGTGCTTCA TTTTGTAGAA CAAAAAATGC AACGCGAGAG CGCTAAATTT TTCAAACAAA  1021 GAAATCTGAG CCTGCATTTT TTTACAGACA GAAATGCAAC GCGGAAGCGC TATTTTTACC 1081 GACGAGATCT GTGCTCATTT TGGTAAAACA AAAAATGCCA AACCGCCCGG AGA //
Primer: Amp_R Sequence file: RDB13124_A5Bgb.seq
>01960_13124_A5Bg_Amp_R_E11_14.ab1     1 TTTTTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT    61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA   121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG   181 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC   241 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC   301 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT   361 GTACTGAGAG TGCACCATAA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT   421 GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT   481 GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT   541 TCCGAAGTTC CTATTCTCTA GCTAGAAAGT ATAGGAACTT CAGAGCGCTT TTGAAAACCA   601 AAAGCGCTCT GAAGACGCAC TTTCAAAAAA CCAAAAACGC ACCGGACTGT AACGAGCTAC   661 TAAAATATTG CGAATACCGC TTCCACAAAC ATTGCTCAAA AGTATCTCTT TGCTATATAT   721 CTCTGTGCTA TATCCCTATA TAACCTACCC ATCCACCTTT CGCTCCTTGA ACTTGCATCT   781 AAACTCGACC TCTACATTTT TTATGTTTAT CTCTAGTATT ACTCTTTAGA CAAAAAAAAT   841 TGTAGTAAGA ACTATTCATA GAGTGAATCG AAAACAATAC GAAAATGTAA ACATTTCCTA   901 TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA ATTTTCTGAC CAATGAAGAA   961 TCATCAACGC TATCACTTTC TGTTCACAAA GTATGCGCAA TCCACATCGG TATAGAAATA  1021 TAAATCGGGG ATGCCCTTTA TCTTGAAAAA AATGCACCCG CAGCTTTCGC TAGTAATCAG  1081 TAAAACGGCG GGGAAGTCAA GTTCAAGCCT TTTTTATGGA AGAGAAAATA GGACCACCCA 1141 AAAGGTTAAG C //
Primer: TRP1pro_F Sequence file: RDB13124_A5Bgc.seq
>01960_13124_A5Bg_TRP1pro_F_F11_17.ab1     1 GCCGTCATAG CCACAAGGCA GCTTGGAGTA TGTCTGTTAT TAATTTCACA GGTAGTTCTG    61 GTCCATTGGT GAAAGTTTGC GGCTTGCAGA GCACAGAGGC CGCAGAATGT GCTCTAGATT   121 CCGATGCTGA CTTGCTGGGT ATTATATGTG TGCCCAATAG AAAGAGAACA ATTGACCCGG   181 TTATTGCAAG GAAAATTTCA AGTCTTGTAA AAGCATATAA AAATAGTTCA GGCACTCCGA   241 AATACTTGGT TGGCGTGTTT CGTAATCAAC CTAAGGAGGA TGTTTTGGCT CTGGTCAATG   301 ATTACGGCAT TGATATCGTC CAACTGCATG GAGATGAGTC GTGGCAAGAA TACCAAGAGT   361 TCCTCGGTTT GCCAGTTATT AAAAGACTCG TATTTCCAAA AGACTGCAAC ATACTACTCA   421 GTGCAGCTTC ACAGAAACCT CATTCGTTTA TTCCCTTGTT TGATTCAGAA GCAGGTGGGA   481 CAGGTGAACT TTTGGATTGG AACTCGATTT CTGACTGGGT TGGAAGGCAA GAGAGCCCCG   541 AAAGCTTACA TTTTATGTTA GCTGGTGGAC TGACGCCAGA AAATGTTGGT GATGCGCTTA   601 GATTAAATGG CGTTATTGGT GTTGATGTAA GCGGAGGTGT GGAGACAAAT GGTGTAAAAG   661 ACTCTAACAA AATAGCAAAT TTCGTCAAAA ATGCTAAGAA ATAGGTTATT ACTGAGTAGT   721 ATTTATTTAA GTATTGTTTG TGCACTTGCC GGTAGAGGTG TGGTCAATAA GAGCGACCTC   781 ATGCTATACC TGAGAAAGCA ACCTGACCTA CAGGAAAGAG TTACTCAAGA ATAAGAATTT   841 TCGTTTTAAA ACCTAAGAGT CACTTTAAAA TTTGTATACA CTTATTTTTT TTTATAACTT   901 ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCCCG GAAATTAGCT TGGCTGCAGG   961 TCGACTCACA GATCCCTCTT CGGGAATCAG TTTCTGTTTC AGAATTTCGA GCTCGGGTAC  1021 CCGGGGGATC CCTCTAGAGT CAATTTCGAT CCCGGGGTTT TTTTCTCCTT TGAACGTAAG  1081 TATAGAGGTA TTATCACAAT TTTTTGGTGG ATACCTTTTA ATTACATTTT GAATAAAGGA 1141 AGGTTAATTA //

Please visit Sequencing and PCR primers for primer information.

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References

Original, user report and related articles

original	Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043. [link to RRC of NBRP]
user_report	Yoshimoto, N., Cytokine-dependent activation of JAK-STAT pathway in Saccharomyces cerevisiae. Biotechnol. Bioeng. 113 (8): 1796-1804 (2016). PMID 26853220. [link to RRC of NBRP]
user_report	Ichikawa K., A Novel Yeast-Based Reporter Assay System for the Sensitive Detection of Genotoxic Agents Mediated by a DNA Damage-Inducible LexA-GAL4 Protein. J. Biochem. (Tokyo)., 139, 105-112 (2006). PMID 16428325. [link to RRC of NBRP]

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