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pGMT20

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01960
Resource name pGMT20
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, TRP1, GAL1-10 promoter, Ampicillin
Vector backbone pGMT20 (Plasmid)
Size of vector backbone 5,432 bp
Selectable markers Amp^r (E. coli), TRP1 (S. cerevisiae)
Depositor Iha, Hidekatsu |

Distribution information

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RDB01960 pGMT20 DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Sequence RDB01960z.seq from Depositor
Electronic file Map RDB01960.gif from Depositor
Electronic file Map RDB01960z.gif from Depositor

Featured content

Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13124_A5Bgp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: ADH_rev RDB13124_A5Bga.seq
>01960_13124_A5Bg_ADHrev_D11_11.ab1
    1 TGGAGTCTCA AGATAAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA
   61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCCCGG
  121 AATTAGCTTG GCTGCAGGTC GACTCACAGA TCCTCTTCGG AAATCAGTTT CTGTTCAGAA
  181 TTCGAGCTCG GTACCCGGGG ATCCTCTAGA GTCAATTCGA TCCGGGGTTT TTTCTCCTTG
  241 ACGTTAAAGT ATAGAGGTAT ATTAACAATT TTTTGTTGAT ACTTTTATTA CATTTGAATA
  301 AGAAGTAATA CAAACCGAAA ATGTTGAAAG TATTAGTTAA AGTGGTTATG CAGTTTTTGC
  361 ATTTATATAT CTGTTAATAG ATCAAAAATC ATCGCTTCGC TGATTAATTA CCCCAGAAAT
  421 AAGGCTAAAA AACTAATCGC ATTATCATCC TATGGTTGTT AATTTGATTC GTTCATTTGA
  481 AGGTTTGTGG GGCCAGGTTA CTGCCAATTT TTCCTCTTCA TAACCATAAA AGCTAGTATT
  541 GTAGAATCTT TATTGTTCGG AGCAGTGCGG CGCGAGGCAC ATCTGCGTTT CAGGAACGCG
  601 ACCGGTGAAG ACGAGGACGC ACGGAGGAGA GTCTTCCTTC GGAGGGCTGT CACCCGCTCG
  661 GCGGCTTCTA ATCCGTACTT CAATATAGCA ATGAGCAGTT AAGCGTATTA CTGAAAGTTC
  721 CAAAGAGAAG GTTTTTTTAG GCTAAGATAA TGGGGCTCTT TACATTTCCA CAACATATAA
  781 GTAAGATTAG ATATGGATAT GTATATGGAT ATGTATATGG TGGTAATGCC ATGTAATATG
  841 ATTATTAAAC TTCTTTGCGT CCATCCAAAA AAAAAAGTAA GAATTTTTTG AAAATTCAAT
  901 ATAAATGACA GCTCAGTTAC AAAGTGAAAG TGACCTGCAG GCATGCAAGC AACGAAGCAT
  961 CTGTGCTTCA TTTTGTAGAA CAAAAAATGC AACGCGAGAG CGCTAAATTT TTCAAACAAA
 1021 GAAATCTGAG CCTGCATTTT TTTACAGACA GAAATGCAAC GCGGAAGCGC TATTTTTACC
1081 GACGAGATCT GTGCTCATTT TGGTAAAACA AAAAATGCCA AACCGCCCGG AGA
//
Primer: Amp_R RDB13124_A5Bgb.seq
>01960_13124_A5Bg_Amp_R_E11_14.ab1
    1 TTTTTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
   61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
  121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
  181 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC
  241 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC
  301 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT
  361 GTACTGAGAG TGCACCATAA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT
  421 GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT
  481 GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT
  541 TCCGAAGTTC CTATTCTCTA GCTAGAAAGT ATAGGAACTT CAGAGCGCTT TTGAAAACCA
  601 AAAGCGCTCT GAAGACGCAC TTTCAAAAAA CCAAAAACGC ACCGGACTGT AACGAGCTAC
  661 TAAAATATTG CGAATACCGC TTCCACAAAC ATTGCTCAAA AGTATCTCTT TGCTATATAT
  721 CTCTGTGCTA TATCCCTATA TAACCTACCC ATCCACCTTT CGCTCCTTGA ACTTGCATCT
  781 AAACTCGACC TCTACATTTT TTATGTTTAT CTCTAGTATT ACTCTTTAGA CAAAAAAAAT
  841 TGTAGTAAGA ACTATTCATA GAGTGAATCG AAAACAATAC GAAAATGTAA ACATTTCCTA
  901 TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA ATTTTCTGAC CAATGAAGAA
  961 TCATCAACGC TATCACTTTC TGTTCACAAA GTATGCGCAA TCCACATCGG TATAGAAATA
 1021 TAAATCGGGG ATGCCCTTTA TCTTGAAAAA AATGCACCCG CAGCTTTCGC TAGTAATCAG
 1081 TAAAACGGCG GGGAAGTCAA GTTCAAGCCT TTTTTATGGA AGAGAAAATA GGACCACCCA
1141 AAAGGTTAAG C
//
Primer: TRP1pro_F RDB13124_A5Bgc.seq
>01960_13124_A5Bg_TRP1pro_F_F11_17.ab1
    1 GCCGTCATAG CCACAAGGCA GCTTGGAGTA TGTCTGTTAT TAATTTCACA GGTAGTTCTG
   61 GTCCATTGGT GAAAGTTTGC GGCTTGCAGA GCACAGAGGC CGCAGAATGT GCTCTAGATT
  121 CCGATGCTGA CTTGCTGGGT ATTATATGTG TGCCCAATAG AAAGAGAACA ATTGACCCGG
  181 TTATTGCAAG GAAAATTTCA AGTCTTGTAA AAGCATATAA AAATAGTTCA GGCACTCCGA
  241 AATACTTGGT TGGCGTGTTT CGTAATCAAC CTAAGGAGGA TGTTTTGGCT CTGGTCAATG
  301 ATTACGGCAT TGATATCGTC CAACTGCATG GAGATGAGTC GTGGCAAGAA TACCAAGAGT
  361 TCCTCGGTTT GCCAGTTATT AAAAGACTCG TATTTCCAAA AGACTGCAAC ATACTACTCA
  421 GTGCAGCTTC ACAGAAACCT CATTCGTTTA TTCCCTTGTT TGATTCAGAA GCAGGTGGGA
  481 CAGGTGAACT TTTGGATTGG AACTCGATTT CTGACTGGGT TGGAAGGCAA GAGAGCCCCG
  541 AAAGCTTACA TTTTATGTTA GCTGGTGGAC TGACGCCAGA AAATGTTGGT GATGCGCTTA
  601 GATTAAATGG CGTTATTGGT GTTGATGTAA GCGGAGGTGT GGAGACAAAT GGTGTAAAAG
  661 ACTCTAACAA AATAGCAAAT TTCGTCAAAA ATGCTAAGAA ATAGGTTATT ACTGAGTAGT
  721 ATTTATTTAA GTATTGTTTG TGCACTTGCC GGTAGAGGTG TGGTCAATAA GAGCGACCTC
  781 ATGCTATACC TGAGAAAGCA ACCTGACCTA CAGGAAAGAG TTACTCAAGA ATAAGAATTT
  841 TCGTTTTAAA ACCTAAGAGT CACTTTAAAA TTTGTATACA CTTATTTTTT TTTATAACTT
  901 ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCCCG GAAATTAGCT TGGCTGCAGG
  961 TCGACTCACA GATCCCTCTT CGGGAATCAG TTTCTGTTTC AGAATTTCGA GCTCGGGTAC
 1021 CCGGGGGATC CCTCTAGAGT CAATTTCGAT CCCGGGGTTT TTTTCTCCTT TGAACGTAAG
 1081 TATAGAGGTA TTATCACAAT TTTTTGGTGG ATACCTTTTA ATTACATTTT GAATAAAGGA
1141 AGGTTAATTA
//

Please visit Sequencing and PCR primers for primer information.


References

References

original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.
user report Yoshimoto, N., Cytokine-dependent activation of JAK-STAT pathway in Saccharomyces cerevisiae. Biotechnol. Bioeng. 113 (8): 1796-1804 (2016). PMID 26853220.
user report Ichikawa K., A Novel Yeast-Based Reporter Assay System for the Sensitive Detection of Genotoxic Agents Mediated by a DNA Damage-Inducible LexA-GAL4 Protein. J. Biochem. (Tokyo)., 139, 105-112 (2006). PMID 16428325.

2018.12.10

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