Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pGMT20

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01960
Resource name pGMT20
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, TRP1, GAL1-10 promoter, Ampicillin
Vector backbone pGMT20 (Plasmid)
Size of vector backbone 5,432 bp
Selectable markers Amp^r (E. coli), TRP1 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
Other clones in our bank

External Database

            Reference sequence
              

            Distribution information

            Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
            Terms and conditions set forth by the DEPOSITOR Acknowledgement to Depositor and Developer in publication.
            Ordering Please visit Information of Request for Distribution.[link] 
            Order form 
            [Credit Card Payment]  [Bank Transfer Payment]
            Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
            提供案内 (日本国内) [open/close]

            提供条件 研究結果の発表時、謝辞に寄託者および樹立者名を入れること。
            提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
            提供依頼書 [Word]
            提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

            Catalog # Resource name Shipping form Fee (non-profit org.)
            RDB01960 pGMT20 DNA solution Please contact us.

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01960z.seq
            Remarks, protocol and/or map (gif) RDB01960.gif
            Remarks, protocol and/or map (gif) RDB01960z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13124_A5Bgp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH_rev
            Sequence file: RDB13124_A5Bga.seq check
            >01960_13124_A5Bg_ADHrev_D11_11.ab1
                1 TGGAGTCTCA AGATAAGATT TTCGTTTTAA ACCTAAGAGT CACTTTAAAA TTTGTATACA
               61 CTTATTTTTT TTATAACTTA TTTAATAATA AAAATCATAA ATCATAAGAA ATTCGCCCGG
              121 AATTAGCTTG GCTGCAGGTC GACTCACAGA TCCTCTTCGG AAATCAGTTT CTGTTCAGAA
              181 TTCGAGCTCG GTACCCGGGG ATCCTCTAGA GTCAATTCGA TCCGGGGTTT TTTCTCCTTG
              241 ACGTTAAAGT ATAGAGGTAT ATTAACAATT TTTTGTTGAT ACTTTTATTA CATTTGAATA
              301 AGAAGTAATA CAAACCGAAA ATGTTGAAAG TATTAGTTAA AGTGGTTATG CAGTTTTTGC
              361 ATTTATATAT CTGTTAATAG ATCAAAAATC ATCGCTTCGC TGATTAATTA CCCCAGAAAT
              421 AAGGCTAAAA AACTAATCGC ATTATCATCC TATGGTTGTT AATTTGATTC GTTCATTTGA
              481 AGGTTTGTGG GGCCAGGTTA CTGCCAATTT TTCCTCTTCA TAACCATAAA AGCTAGTATT
              541 GTAGAATCTT TATTGTTCGG AGCAGTGCGG CGCGAGGCAC ATCTGCGTTT CAGGAACGCG
              601 ACCGGTGAAG ACGAGGACGC ACGGAGGAGA GTCTTCCTTC GGAGGGCTGT CACCCGCTCG
              661 GCGGCTTCTA ATCCGTACTT CAATATAGCA ATGAGCAGTT AAGCGTATTA CTGAAAGTTC
              721 CAAAGAGAAG GTTTTTTTAG GCTAAGATAA TGGGGCTCTT TACATTTCCA CAACATATAA
              781 GTAAGATTAG ATATGGATAT GTATATGGAT ATGTATATGG TGGTAATGCC ATGTAATATG
              841 ATTATTAAAC TTCTTTGCGT CCATCCAAAA AAAAAAGTAA GAATTTTTTG AAAATTCAAT
              901 ATAAATGACA GCTCAGTTAC AAAGTGAAAG TGACCTGCAG GCATGCAAGC AACGAAGCAT
              961 CTGTGCTTCA TTTTGTAGAA CAAAAAATGC AACGCGAGAG CGCTAAATTT TTCAAACAAA
             1021 GAAATCTGAG CCTGCATTTT TTTACAGACA GAAATGCAAC GCGGAAGCGC TATTTTTACC
            1081 GACGAGATCT GTGCTCATTT TGGTAAAACA AAAAATGCCA AACCGCCCGG AGA
            //
            Primer: Amp_R
            Sequence file: RDB13124_A5Bgb.seq check
            >01960_13124_A5Bg_Amp_R_E11_14.ab1
                1 TTTTTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
               61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
              121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
              181 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC
              241 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC
              301 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT
              361 GTACTGAGAG TGCACCATAA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT
              421 GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT
              481 GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT
              541 TCCGAAGTTC CTATTCTCTA GCTAGAAAGT ATAGGAACTT CAGAGCGCTT TTGAAAACCA
              601 AAAGCGCTCT GAAGACGCAC TTTCAAAAAA CCAAAAACGC ACCGGACTGT AACGAGCTAC
              661 TAAAATATTG CGAATACCGC TTCCACAAAC ATTGCTCAAA AGTATCTCTT TGCTATATAT
              721 CTCTGTGCTA TATCCCTATA TAACCTACCC ATCCACCTTT CGCTCCTTGA ACTTGCATCT
              781 AAACTCGACC TCTACATTTT TTATGTTTAT CTCTAGTATT ACTCTTTAGA CAAAAAAAAT
              841 TGTAGTAAGA ACTATTCATA GAGTGAATCG AAAACAATAC GAAAATGTAA ACATTTCCTA
              901 TACGTAGTAT ATAGAGACAA AATAGAAGAA ACCGTTCATA ATTTTCTGAC CAATGAAGAA
              961 TCATCAACGC TATCACTTTC TGTTCACAAA GTATGCGCAA TCCACATCGG TATAGAAATA
             1021 TAAATCGGGG ATGCCCTTTA TCTTGAAAAA AATGCACCCG CAGCTTTCGC TAGTAATCAG
             1081 TAAAACGGCG GGGAAGTCAA GTTCAAGCCT TTTTTATGGA AGAGAAAATA GGACCACCCA
            1141 AAAGGTTAAG C
            //
            Primer: TRP1pro_F
            Sequence file: RDB13124_A5Bgc.seq check
            >01960_13124_A5Bg_TRP1pro_F_F11_17.ab1
                1 GCCGTCATAG CCACAAGGCA GCTTGGAGTA TGTCTGTTAT TAATTTCACA GGTAGTTCTG
               61 GTCCATTGGT GAAAGTTTGC GGCTTGCAGA GCACAGAGGC CGCAGAATGT GCTCTAGATT
              121 CCGATGCTGA CTTGCTGGGT ATTATATGTG TGCCCAATAG AAAGAGAACA ATTGACCCGG
              181 TTATTGCAAG GAAAATTTCA AGTCTTGTAA AAGCATATAA AAATAGTTCA GGCACTCCGA
              241 AATACTTGGT TGGCGTGTTT CGTAATCAAC CTAAGGAGGA TGTTTTGGCT CTGGTCAATG
              301 ATTACGGCAT TGATATCGTC CAACTGCATG GAGATGAGTC GTGGCAAGAA TACCAAGAGT
              361 TCCTCGGTTT GCCAGTTATT AAAAGACTCG TATTTCCAAA AGACTGCAAC ATACTACTCA
              421 GTGCAGCTTC ACAGAAACCT CATTCGTTTA TTCCCTTGTT TGATTCAGAA GCAGGTGGGA
              481 CAGGTGAACT TTTGGATTGG AACTCGATTT CTGACTGGGT TGGAAGGCAA GAGAGCCCCG
              541 AAAGCTTACA TTTTATGTTA GCTGGTGGAC TGACGCCAGA AAATGTTGGT GATGCGCTTA
              601 GATTAAATGG CGTTATTGGT GTTGATGTAA GCGGAGGTGT GGAGACAAAT GGTGTAAAAG
              661 ACTCTAACAA AATAGCAAAT TTCGTCAAAA ATGCTAAGAA ATAGGTTATT ACTGAGTAGT
              721 ATTTATTTAA GTATTGTTTG TGCACTTGCC GGTAGAGGTG TGGTCAATAA GAGCGACCTC
              781 ATGCTATACC TGAGAAAGCA ACCTGACCTA CAGGAAAGAG TTACTCAAGA ATAAGAATTT
              841 TCGTTTTAAA ACCTAAGAGT CACTTTAAAA TTTGTATACA CTTATTTTTT TTTATAACTT
              901 ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCCCG GAAATTAGCT TGGCTGCAGG
              961 TCGACTCACA GATCCCTCTT CGGGAATCAG TTTCTGTTTC AGAATTTCGA GCTCGGGTAC
             1021 CCGGGGGATC CCTCTAGAGT CAATTTCGAT CCCGGGGTTT TTTTCTCCTT TGAACGTAAG
             1081 TATAGAGGTA TTATCACAAT TTTTTGGTGG ATACCTTTTA ATTACATTTT GAATAAAGGA
            1141 AGGTTAATTA
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.
            user report Yoshimoto, N., Cytokine-dependent activation of JAK-STAT pathway in Saccharomyces cerevisiae. Biotechnol. Bioeng. 113 (8): 1796-1804 (2016). PMID 26853220.
            user report Ichikawa K., A Novel Yeast-Based Reporter Assay System for the Sensitive Detection of Genotoxic Agents Mediated by a DNA Damage-Inducible LexA-GAL4 Protein. J. Biochem. (Tokyo)., 139, 105-112 (2006). PMID 16428325.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl