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pAMU20

E. coli - S. cerevisiae shuttle vector

Catalog number RDB01954
Resource name pAMU20
Alternative name E. coli-S. cerevisiae shuttle vector
Clone info. E. coli-S. cerevisiae shuttle vector, 9E10 epitope (Myc-tag), 2micron ori, URA3, ADH1 promoter, Ampicillin
Vector backbone pAMU20 (Plasmid)
Size of vector backbone 5,367 bp
Selectable markers Amp^r (E. coli), URA3 (S. cerevisiae)
Depositor|Developer Iha, Hidekatsu |
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External Database

            Reference sequence
              

            Distribution information

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            RDB01954 pAMU20 DNA solution

            Ordering Information [in Japanese] [in English]
            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Sequence RDB01954z.seq
            Remarks, protocol and/or map (gif) RDB01954.gif
            Remarks, protocol and/or map (gif) RDB01954z.gif

            Original reference

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            Further references such as user reports and related articles (go to bottom)

            Featured content

            Featured content Expression Vector Backbone of Saccharomyces cerevisiae (English text)

            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB12314_A5Bg.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: ADH_rev
            Sequence file: RDB12314_A5Bga.seq check
            >01954_12314_A5Bg_ADHrev_D07_10.ab1
                1 TGAGGTCTCA AGATAGATTT TCGTTTTAAA CCTAAGAGTC ACCCTCTAAA ATTTGTATAC
               61 ACTTATTTTT TTTATAACTT ATTTAATAAT AAAAATCATA AATCATAAGA AATTCGCCCG
              121 GAATTAGCTT GGCTGCAGGT CGACTCACAG ATCCTCTTCG GAAATCAGTT TCTGTTCAGA
              181 ATTCGAGCTC GGTACCCGGG GATCCTCTAG AGTCAGCTTG GAGTTGATTG TATGCTTGGT
              241 ATAGCTTGAA ATATTGTGCA GAAAAAGAAA CAAGGAAGAA AGGGAACGAG AACAATGACG
              301 AGGAAACAAA AGATTAATAA TTGCAGGTCT ATTTATACTT GATAGCAAGA CAGCAAACTT
              361 TTTTTTATTT CAAATTCAAG TAACTGGAAG GAAGGCCGTA TACCGTTGCT CATTAGAGAG
              421 TAGTGTGCGT GAATGAAGGA AGGAAAAAGT TTCGTGTGCT TCGAGATACC CCTCATCAGC
              481 TCTGGAACAA CGACATCTGT TGGTGCTGTC TTTGTCGTTA ATTTTTTCCT TTAGTGTCTT
              541 CCATCATTTT TTTGTCATTG CGGATATGGT GAGACAACAA CGGGGGAGAG AGAAAAGAAA
              601 AAAAAAGAAA AGAAGTTGCA TGCAAGCAAC GAAGCATCTG TGCTTCATTT TGTAGAACAA
              661 AAATGCAACG CGAGAGCGCT AATTTTTCAA ACAAAGAATC TGAGCTGCAT TTTTACAGAA
              721 CAGAAATGCA ACGCGAAAGC GCTATTTTAC CAACGAAGAA TCTGTGCTTC ATTTTTGTAA
              781 AACAAAAATG CAACGCGAGA GCGCTAATTT TTCAAACAAA GAATCTGAGC TGCATTTTTA
              841 CAGAACAGAA ATGCAACGCG AGAGCGCTAT TTTACCAACA AAGAATCTAT ACTTCTTTTT
              901 TTGTTCTACA AAAATGCATC CCGAGAGCGC TATTTTTTCT AACAAAGCAT CTTTAGATTA
              961 CTTTTTTTTC TCCCTTTGTG CGCTCTATAA ATGCAGTCTC TTTGATAACT TTTTGCACTG
             1021 TAGGTCCCGT TTAAAGTTAG AGAATGCTAC TTTGGATGTC TATTTTCTTC TCATAAAAAA
            1081 AGTCTTGACT TCCACTGCCC GCGATTACTG AATAACTAGC GAAGCTGCCG GGGATTC
            //
            Primer: Amp_R
            Sequence file: RDB12314_A5Bgb.seq check
            >01954_12314_A5Bg_Amp_R_E07_13.ab1
                1 TCTTTTTTCA TATTATTGAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT
               61 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA
              121 CCTGACGTCT AAGAAACCAT TATTATCATG ACATTAACCT ATAAAAATAG GCGTATCACG
              181 AGGCCCTTTC GTCTCGCGCG TTTCGGTGAT GACGGTGAAA ACCTCTGACA CATGCAGCTC
              241 CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA GCAGACAAGC CCGTCAGGGC
              301 GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC TGGCTTAACT ATGCGGCATC AGAGCAGATT
              361 GTACTGAGAG TGCACCATAA CGCATTTAAG CATAAACACG CACTATGCCG TTCTTCTCAT
              421 GTATATATAT ATACAGGCAA CACGCAGATA TAGGTGCGAC GTGAACAGTG AGCTGTATGT
              481 GCGCAGCTCG CGTTGCATTT TCGGAAGCGC TCGTTTTCGG AAACGCTTTG AAGTTCCTAT
              541 TCCGAAGTTC CTATTCTCTA GCTAGAAAGT ATAGGAACTT CAGAGCGCTT TTGAAAACCA
              601 AAAGCGCTCT GAAGACGCAC TTTCAAAAAA CCAAAAACGC ACCGGACTGT AACGAGCTAC
              661 TAAAATATTG CGAATACCGC TTCCACAAAC ATTGCTCAAA AGTATCTCTT TGCTATATAT
              721 CTCTGTGCTA TATCCCTATA TAACCTACCC ATCCACCTTT CGCTCCTTGA ACTTGCATCT
              781 AAACTCGACC TCTACATTTT TTATGTTTAT CTCTAGTATT ACTCTTTAGA CAAAAAAATT
              841 GTAGTAAGAA CTATTCATAG AGTGAATCGA AAACAATACG AAAATGTAAA CATTTCCTAT
              901 ACGTAGTATA TAGAGACAAA ATAGAAGAAA CCGTTCATAA TTTTCTGACC AATGAAGAAT
              961 CATCAACGCT ATCACTTTCT GTTCACAAAG TATGCGCAAT CCACATCGGT ATAGAATATA
             1021 AATCGGGGAT GCCCTTTATC TTGAAAAAAT GCACCCGCAG CTTCGCTAGT ATCCAGTAAC
             1081 CGCCGGCAGT TGCAGGTTCA AGCCTTTTTT TATGCAAGAG AAATGAACTC AAGCTAGCGA
            1141 TCGTTCCCTA
            //
            Primer: URA3pro_F
            Sequence file: RDB12314_A5Bgc.seq check
            >01954_12314_A5Bg_URA3pro_F_F07_16.ab1
                1 CTAATTCTAG TGTTGAGAAC ATGAATTGCC CAGTATTCTT AACCCAACTG CACAGAACAA
               61 AAACCTGCAG GAAACGAAGA TAAATCATGT CGAAAGCTAC ATATAAGGAA CGTGCTGCTA
              121 CTCATCCTAG TCCTGTTGCT GCCAAGCTAT TTAATATCAT GCACGAAAAG CAAACAAACT
              181 TGTGTGCTTC ATTGGATGTT CGTACCACCA AGGAATTACT GGAGTTAGTT GAAGCATTAG
              241 GTCCCAAAAT TTGTTTACTA AAAACACATG TGGATATCTT GACTGATTTT TCCATGGAGG
              301 GCACAGTTAA GCCGCTAAAG GCATTATCCG CCAAGTACAA TTTTTTACTC TTCGAAGACA
              361 GAAAATTTGC TGACATTGGT AATACAGTCA AATTGCAGTA CTCTGCGGGT GTATACAGAA
              421 TAGCAGAATG GGCAGACATT ACGAATGCAC ACGGTGTGGT GGGCCCAGGT ATTGTTAGCG
              481 GTTTGAAGCA GGCGGCAGAA GAAGTAACAA AGGAACCTAG AGGCCTTTTG ATGTTAGCAG
              541 AATTGTCATG CAAGGGCTCC CTATCTACTG GAGAATATAC TAAGGGTACT GTTGACATTG
              601 CGAAGAGCGA CAAAGATTTT GTTATCGGCT TTATTGCTCA AAGAGACATG GGTGGAAGAG
              661 ATGAAGGTTA CGATTGGTTG ATTATGACAC CCGGTGTGGG TTTAGATGAC AAGGGAGACG
              721 CATTGGGTCA ACAGTATAGA ACCGTGGATG ATGTGGTCTC TACAGGATCT GACATTATTA
              781 TTGTTGGAAG AGGACTATTT GCAAAGGGAA GGGATGCTAA GGTAGAGGGT GAACGTTACA
              841 GAAAAGCAGG CTGGGAAGCA TATTTGAGAA GATGCGGCCA GCAAAACTAA AAAAACTGTA
              901 TTATAAGTAA AAGCTTACAT TTTTATGTTA GCTGGTGGAC TGACGCCAGA AAATGTTGGT
              961 GATGCGCTTA GATTAAATGG CGTTATTGGT GTTGATGTAA GCGGGAGGTG TGGAGACAAA
             1021 TGGGTGTAAA AGACTTCTAA CAAAATAGCA AATTTTCGTC AAAATGCTAG AATAGGTTAT
             1081 TTACTGAGTT AGTATTTTAT TTCAGGTATT GCTTTGTGCA CTTGACGGGG TTAGAAGGGT
            1141 GTGTTGTGG
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Iha, H., Shuttle-vector system for Saccharomyces cerevisiae designed to produce C-terminal-Myc-tagged fusion proteins. Biotechniques, 25, 936-938 (1998). PMID 9863043.

            2022.02.09

            GNP_filter3_RDBDEP_html_220112.pl