Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pcDNA3-XPF (#RDB01785)

Human ERCC4 cDNA

Alternative name ERCC4
Clone info. Human ERCC4 cDNA; Expression in mammalian cell
Vector backbone pcDNA3 (Plasmid)
Size of vector backbone 5,446 bp
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human XPF cDNA
Depositor|Developer Takebe, Hiraku | Yagi, Takashi |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01785 pcDNA3-XPF DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pcDNA3-XPF was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01785).

Reference section:

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB19983_B3Enp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV_Forward (Pr0016)
Region: insert 5'
Sequence file: RDB19983_B3Ena.seq check
>01785_19983_B3En_2_CMV_Forward_F01_16_ABI24.ab1
    1 NNNNNNNNNN NTNNNNGCNN NNGGGCGGTA NGCGTGTACG GTGGGAGGTC TATATAAGCA
   61 GAGNNNNCTG GCTAACTAGA GAACCCACTG CTTACTGGCT TATCGAAATT AATACGACTC
  121 ACTATAGGGA GACCCAAGCT TGGTACCGGC TCGACGGATT GCCATGGCGC CGCTGCTGGA
  181 GTACGAGCGA CAGCTGGTGC TGGAACTGCT CGACACTGAC GGGCTAGTAG TGTGTGCCCG
  241 CGGGCTCGGC GCGGACCGGC TCCTCTACCA CTTTCTCCAG CTGCACTGCC ACCCAGCCTG
  301 CCTGGTGCTG GTGCTCAACA CGCAGCCGGC CGAGGAGGAG TATTTTATCA ATCAGCTGAA
  361 GATAGAAGGA GTTGAACACC TCCCTCGCCG TGTAACAAAT GAAATCACAA GCAACAGTCG
  421 CTATGAAGTT TACACACAAG GTGGTGTTAT ATTTGCGACA AGTAGGATAC TTGTGGTTGA
  481 CTTCTTGACT GATAGAATAC CTTCAGATTT AATTACTGGC ATCTTGGTGT ATAGAGCCCA
  541 CAGAATAATC GAGTCTTGTC AAGAAGCATT CATCTTGCGC CTCTTTCGCC AGAAAAACAA
  601 ACGTGGTTTT ATTAAAGCTT TCACAGACAA TGCTGTTGCC TTTGATACTG GTTTTTGTCA
  661 TGTGGAAAGA GTGATGAGAA ATCTTTTTGT GANGAAACTG TATCTGTGGC CNANGTTCCA
  721 TGTAGCAGTA AACTCATTTT TAGAACAGCA CAAACCTGAA NNNGTANAAA TCCNTGTTTC
  781 TATGANACCT ACNATGCTNG CTATACNGAC TGCTATACTG GANATTTTAA ATGNNTGTCT
  841 AAAGGAACTA AAATGNCNTA NCCNNTCGCT NGANNNNGNA NNANTTNTNT TNNNAAANNG
901 NTATNNNNAA ANCNTTTNNN NNNNNNN
//
Primer: bGH_rev3 (Pr0885)
Region: bGH pA,insert 3'
Sequence file: RDB19983_B3Enb.seq check
>01785_19983_B3En_2_bGH_rev3_G01_19_ABI24.ab1
    1 NNNNNNNNNN NNGGNNGNNG GGCNNANNAC NGNTGGCTGG CAACTAGAAG GCACAGTCGA
   61 GGCTGATCAG CGAGCTCTAG CATTTAGGTG ACACTATAGA ATAGGGCCCT CTAGATGTCT
  121 GGCAAGGAGC CGCTGAAAAG TACAGGCATG GGATAAGAAA ACAGCCATCA CTGTTCACTT
  181 TTTCCCTTTT CCTTTTGATA CGACTTCTGC AAAAGAGGTG TGAATGAAAT CATAAAGCTG
  241 TTTGGCATTT GCAGCATTCC CCAGAATACT CGTGAGCTCG TCTTGTGACA GGGCTGCTAA
  301 TTCTGCGATG TTCTTAACGT GGTGCATCAA GGAGCGGCAG TTTTTGGCAT TCACCCCTGG
  361 CATTTTTAAC AAGAAGTCTT GGGGACCAGG ATTATACTTC TCTGACTCGG GAAGGGTTTC
  421 AGAATCTGCT GTAATGGCCA GTGCTGTCGC CGCATCAGGC TGTGGCTTGC TTTGTTTCAG
  481 CTCCTCAAAC AACTCCGCCG TTGCATGAGG AGAGGGGCAC CAGAGAATCC GTAGTCTGGG
  541 GAAGTGAAGT GTAAGAAGAG TGAGTTTGGA ACTAATGTCA TTGCTGGAGA TCTCCTGAAA
  601 CAAGGCACCT CGGGAAGTGA GAGAGAAAGG CTTGCTAGGG TCAAACTCAA TCAGAAGCAC
  661 GGGACGCTTG TAGTAGCGGG ACATGGAGAT GCACTGGCTG TAGAGGCGGC CGTTATTTAA
  721 AGAGCCGATT AAATCACTGA TACTCTTGCG CTCCACGCAC ATTTCTGGAG TGAGGNNGTA
  781 NTCTCCNACC TCTAAAGTCA CGGGNTCNAT GTCNATGNCC CGACNATGGN TCAGANNNGG
  841 AAGCTCACTT CNAAANTCAC GCATATNCNC NACNANGCTT TGCTGNGNAC NNNNNNGNNN
901 NNNGNNCNNN NNNNTNNCNA NNGNCANNNG NNNNNNNNNN
//
Primer: SV40pro_F (Pr0110)
Region: SV40 pro_ori,NeoR
Sequence file: RDB19983_B3Enc.seq check
>01785_19983_B3En_2_SV40pro_F_H01_22_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NANNNNTGNN GNNGNTTTTT TGGANGCCTA GGCTTTTGCA
   61 AAAAGCTCCC GGGAGCTTGT ATATCCATTT TCGGATCTGA TCAAGAGACA GGATGAGGAT
  121 CGTTTCGCAT GATTGAACAA GATGGATTGC ACGCAGGTTC TCCGGCCGCT TGGGTGGAGA
  181 GGCTATTCGG CTATGACTGG GCACAACAGA CAATCGGCTG CTCTGATGCC GCCGTGTTCC
  241 GGCTGTCAGC GCAGGGGCGC CCGGTTCTTT TTGTCAAGAC CGACCTGTCC GGTGCCCTGA
  301 ATGAACTGCA GGACGAGGCA GCGCGGCTAT CGTGGCTGGC CACGACGGGC GTTCCTTGCG
  361 CAGCTGTGCT CGACGTTGTC ACTGAAGCGG GAAGGGACTG GCTGCTATTG GGCGAAGTGC
  421 CGGGGCAGGA TCTCCTGTCA TCTCACCTTG CTCCTGCCGA GAAAGTATCC ATCATGGCTG
  481 ATGCAATGCG GCGGCTGCAT ACGCTTGATC CGGCTACCTG CCCATTCGAC CACCAAGCGA
  541 AACATCGCAT CGAGCGAGCA CGTACTCGGA TGGAAGCCGG TCTTGTCGAT CAGGATGATC
  601 TGGACGAAGA GCATCAGGGG CTCGCGCCAG CCGAACTGTT CGCCAGGCTC AAGGCGCGCA
  661 TGCCCGACGG CGAGGATCTC GTCGTGACCC ATGGCGATGC CTGCTTGCCG ANNATCATGG
  721 TGGAAAATGG NCGCTTTTCT GGANTCNTCG ACTGNGGCCG GCTGGGTGTG GCGNANCGNT
  781 ATCANGACAT ANCGTNNGCT ACCCGTGATA NTGCTGAANA NCTTGGCGGC NAATGGGCTG
841 ANCGCTNNCN CNNGNTTTNN NGGNANCNNN NNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

user_report Wang Y., Repair of 2'-C-cyano-2'-deoxy-1-beta-D-arabino-pentofuranosylcytosine-induced DNA single-strand breaks by transcription-coupled nucleotide excision repair. Cancer Res., 68, 3881-3889 (2008). PMID 18483273. [link to RRC of NBRP]