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pRx hCD25iN (#RDB01700)

Retroviral vector of human CD25

Alternative name Rx-hCD25-IRES-Neo
Clone info. Retroviral vector of human CD25. ires, neo
Vector backbone pRxZiN (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human CD25/IL-2 receptor cDNA
Depositor|Developer Hamada, Hirofumi |
 
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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Leukoc. Biol., 60, 181-190 (1996).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks Remember that you will be working with samples containing infectious virus.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Leukoc. Biol., 60, 181-190 (1996).)。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
備考 このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01700 pRx hCD25iN DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pRx hCD25iN was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01700).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13005_A4Jv.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp
Sequence file: RDB13005_A4Jva.seq check
>01700_13005_A4Jv_2_T7_20bp_E12_15.ab1
    1 CTCGTCTGGA GATGGATTCA TACCTGCTGA TGTGGGGACT GCTCACGTTC ATCATGGTGC
   61 CTGGCTGCCA GGCAGAGCTC TGTGACGATG ACCCGCCAGA GATCCCACAC GCCACATTCA
  121 AAGCCATGGC CTACAAGGAA GGAACCATGT TGAACTGTGA ATGCAAGAGA GGTTTCCGCA
  181 GAATAAAAAG CGGGTCACTC TATATGCTCT GTACAGGAAA CTCTAGCCAC TCGTCCTGGG
  241 ACAACCAATG TCAATGCACA AGCTCTGCCA CTCGGAACAC AATGAAACAA GTGACACCTC
  301 AACCTGAAGA ACAGAAAGAA AGGAAAACCA CAGAAATGCA AAGTCCAATG CAGCCAGTGG
  361 ACCAAGCGAG CCTTCCAGGT CACTGCAGGG AACCTCCACC ATGGGAAAAT GAAGCCACAG
  421 AGAGAATTTA TCATTTCGTG GTGGGGCAGA TGGTTTATTA TCAGTGCGTC CAGGGATACA
  481 GGGCTCTACA CAGAGGTCCT GCTGAGAGCG TCTGCAAAAT GACCCACGGG AAGACAAGGT
  541 GGACCCAGCC CCAGCTCATA TGCACAGGTG AAATGGAGAC CAGTCAGTTT CCAGGTGAAG
  601 AGAAGCCTCA GGCAAGCCCC GAAGGCCGTC CTGAGAGTGA GACTTCCTGC CTCGTCACAA
  661 CAACAGATTT TCAAATACAG ACAGAAATGG CTGCAACCAT GGAGACGTCC ATATTTACAA
  721 CAGAGTACCA GGTAGCAGTG GCCGGCTGTG TTTTCCTGCT GATCAGCGTC CTCCTCCTGA
  781 GTGGGCTCAC CTGGCAGCGG AGACAGAGGA AGAGTAGAAG AACAATCTAG CCAGCACAAT
  841 GGCGGCCGCA GTATTCTATA GTGTCACAAT GGATCCTCTA GAGAATTCCG CCCCCCCCTA
901 CCCCCCCTTC ACCC
//
Primer: IRES-F
Sequence file: RDB13005_A4Jvb.seq check
>01700_13005_A4Jv_2_IRES-F_F12_18.ab1
    1 GCATCTCTGT GTTTAGTCGA GGTTAAAAAA CGTCTAGGCC CCCCGAACCA CGGGGACGTG
   61 GTTTTCCTTT GAAAAACACG ATGATAAGCT TGCCACAACC ATGGGGATCG GCCATTGAAC
  121 AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT
  181 GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC
  241 GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG
  301 CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG
  361 TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT
  421 CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC
  481 ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG
  541 CACGTACTCG GATGGAAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG
  601 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT
  661 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT
  721 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG
  781 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT
  841 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC
  901 TTCTGAGGGA TCCGGAGCCA TAGATAAAAT AAAAGATTTT ATTTAGTCTC CAGAAAAAGG
  961 GGGGAATGAA AGACCCCACC TGTAGGTTTG GCAAGCTAGC TTAAGTAACG CCATTTTGCA
 1021 AGGCATGGAA AAATACATAA ACTGAGATAG AGAAGTTTCA GATCAAGGTC AGACAGATGG
 1081 GAACAGCTGA ATATGGGTAA ACAGATATCT GTGGTAAGCA GGTTCCTTGA CCCCCCGGGC
1141 TTCAAGGCCA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Wakimoto, H., Efficient retrovirus-mediated cytokine-gene transduction of primary-cultured human glioma cells for tumor vaccination therapy. Jpn. J. Cancer Res., 88, 296-305 (1997). PMID 9140115.