Resource data sheet
Prev.

pRx hCD25iN

Retroviral vector of human CD25

Catalog number RDB01700
Resource name pRx hCD25iN
Alternative name Rx-hCD25-IRES-Neo
Clone info. Retroviral vector of human CD25. ires, neo
Vector backbone pRxZiN (Plasmid)
Selectable markers Amp^r (E. coli), Neo^r (mammalian cell)
Gene/insert name Human CD25/IL-2 receptor cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human IL2RA (NCBI Gene 3559) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Leukoc. Biol., 60, 181-190 (1996).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Ordering Please visit Information of Request for Distribution. 
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Remarks Remember that you will be working with samples containing infectious virus.
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01700 pRx hCD25iN DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01700.gif from Depositor

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References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13005_A4Jv.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB13005_A4Jva.seq
>01700_13005_A4Jv_2_T7_20bp_E12_15.ab1
    1 CTCGTCTGGA GATGGATTCA TACCTGCTGA TGTGGGGACT GCTCACGTTC ATCATGGTGC
   61 CTGGCTGCCA GGCAGAGCTC TGTGACGATG ACCCGCCAGA GATCCCACAC GCCACATTCA
  121 AAGCCATGGC CTACAAGGAA GGAACCATGT TGAACTGTGA ATGCAAGAGA GGTTTCCGCA
  181 GAATAAAAAG CGGGTCACTC TATATGCTCT GTACAGGAAA CTCTAGCCAC TCGTCCTGGG
  241 ACAACCAATG TCAATGCACA AGCTCTGCCA CTCGGAACAC AATGAAACAA GTGACACCTC
  301 AACCTGAAGA ACAGAAAGAA AGGAAAACCA CAGAAATGCA AAGTCCAATG CAGCCAGTGG
  361 ACCAAGCGAG CCTTCCAGGT CACTGCAGGG AACCTCCACC ATGGGAAAAT GAAGCCACAG
  421 AGAGAATTTA TCATTTCGTG GTGGGGCAGA TGGTTTATTA TCAGTGCGTC CAGGGATACA
  481 GGGCTCTACA CAGAGGTCCT GCTGAGAGCG TCTGCAAAAT GACCCACGGG AAGACAAGGT
  541 GGACCCAGCC CCAGCTCATA TGCACAGGTG AAATGGAGAC CAGTCAGTTT CCAGGTGAAG
  601 AGAAGCCTCA GGCAAGCCCC GAAGGCCGTC CTGAGAGTGA GACTTCCTGC CTCGTCACAA
  661 CAACAGATTT TCAAATACAG ACAGAAATGG CTGCAACCAT GGAGACGTCC ATATTTACAA
  721 CAGAGTACCA GGTAGCAGTG GCCGGCTGTG TTTTCCTGCT GATCAGCGTC CTCCTCCTGA
  781 GTGGGCTCAC CTGGCAGCGG AGACAGAGGA AGAGTAGAAG AACAATCTAG CCAGCACAAT
  841 GGCGGCCGCA GTATTCTATA GTGTCACAAT GGATCCTCTA GAGAATTCCG CCCCCCCCTA
901 CCCCCCCTTC ACCC
//
Primer: IRES-F RDB13005_A4Jvb.seq
>01700_13005_A4Jv_2_IRES-F_F12_18.ab1
    1 GCATCTCTGT GTTTAGTCGA GGTTAAAAAA CGTCTAGGCC CCCCGAACCA CGGGGACGTG
   61 GTTTTCCTTT GAAAAACACG ATGATAAGCT TGCCACAACC ATGGGGATCG GCCATTGAAC
  121 AAGATGGATT GCACGCAGGT TCTCCGGCCG CTTGGGTGGA GAGGCTATTC GGCTATGACT
  181 GGGCACAACA GACAATCGGC TGCTCTGATG CCGCCGTGTT CCGGCTGTCA GCGCAGGGGC
  241 GCCCGGTTCT TTTTGTCAAG ACCGACCTGT CCGGTGCCCT GAATGAACTG CAGGACGAGG
  301 CAGCGCGGCT ATCGTGGCTG GCCACGACGG GCGTTCCTTG CGCAGCTGTG CTCGACGTTG
  361 TCACTGAAGC GGGAAGGGAC TGGCTGCTAT TGGGCGAAGT GCCGGGGCAG GATCTCCTGT
  421 CATCTCACCT TGCTCCTGCC GAGAAAGTAT CCATCATGGC TGATGCAATG CGGCGGCTGC
  481 ATACGCTTGA TCCGGCTACC TGCCCATTCG ACCACCAAGC GAAACATCGC ATCGAGCGAG
  541 CACGTACTCG GATGGAAAGC CGGTCTTGTC GATCAGGATG ATCTGGACGA AGAGCATCAG
  601 GGGCTCGCGC CAGCCGAACT GTTCGCCAGG CTCAAGGCGC GCATGCCCGA CGGCGAGGAT
  661 CTCGTCGTGA CCCATGGCGA TGCCTGCTTG CCGAATATCA TGGTGGAAAA TGGCCGCTTT
  721 TCTGGATTCA TCGACTGTGG CCGGCTGGGT GTGGCGGACC GCTATCAGGA CATAGCGTTG
  781 GCTACCCGTG ATATTGCTGA AGAGCTTGGC GGCGAATGGG CTGACCGCTT CCTCGTGCTT
  841 TACGGTATCG CCGCTCCCGA TTCGCAGCGC ATCGCCTTCT ATCGCCTTCT TGACGAGTTC
  901 TTCTGAGGGA TCCGGAGCCA TAGATAAAAT AAAAGATTTT ATTTAGTCTC CAGAAAAAGG
  961 GGGGAATGAA AGACCCCACC TGTAGGTTTG GCAAGCTAGC TTAAGTAACG CCATTTTGCA
 1021 AGGCATGGAA AAATACATAA ACTGAGATAG AGAAGTTTCA GATCAAGGTC AGACAGATGG
 1081 GAACAGCTGA ATATGGGTAA ACAGATATCT GTGGTAAGCA GGTTCCTTGA CCCCCCGGGC
1141 TTCAAGGCCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

reference Wakimoto, H., Efficient retrovirus-mediated cytokine-gene transduction of primary-cultured human glioma cells for tumor vaccination therapy. Jpn. J. cancer Res., 88, 296-305 (1997). PMID 9140115.

2018.12.10

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