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pCM-hGM

Human CM-hGM-CSF cDNA

Catalog number RDB01687
Resource name pCM-hGM
Alternative name CM-hGM-CSF, pCM-hGM
Clone info. Human GM-CSF expression under regulation of CM promoter
Vector backbone pCM-NtTA (Plasmid)
Size of vector backbone 3,875 bp
Selectable markers Amp^r
Gene/insert name Human GM-CSF cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank

External Database
Human GM-CSF

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Leukoc. Biol., 60, 181-190 (1996).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Leukoc. Biol., 60, 181-190 (1996).。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01687 pCM-hGM DNA solution Please contact us.

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01687.gif

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12796_A4Es.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: CMV-Forward
          Sequence file: RDB12796_A4Esa.seq check
          >01687_12796_A4Es_CMV-Foward_C07_07.ab1
              1 TTTTTCCACT TCCGCCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
             61 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT
            121 TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG CGGCCCCGAA TTCTAGACCA
            181 CCATGTGGCT GCAGAGCCTG CTGCTCTTGG GCACTGTGGC CTGCAGCATC TCTGCACCCG
            241 CCCGCTCGCC CAGCCCCAGC ACGCAGCCCT GGGAGCATGT GAATGCCATC CAGGAGGCCC
            301 GGCGTCTCCT GAACCTGAGT AGAGACACTG CTGCTGAGAT GAATGAAACA GTAGAAGTCA
            361 TCTCAGAAAT GTTTGACCTC CAGGAGCCGA CCTGCCTACA GACCCGCCTG GAGCTGTACA
            421 AGCAGGGCCT GCGGGGCAGC CTCACCAAGC TCAAGGGCCC CTTGACCATG ATGGCCAGCC
            481 ACTACAAGCA GCACTGCCCT CCAACCCCGG AAACTTCCTG TGCAACCCAG ACTATCACCT
            541 TTGAAAGTTT CAAAGAGAAC CTGAAGGACT TTCTGCTTGT CATCCCCTTT GACTGCTGGG
            601 AGCCAGTCCA GGAGTGAGGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC
            661 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
            721 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG
            781 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT
            841 GGTATGGCTG ATTATGATCC TGCAAGCCTC GTCGTCCTGG CCGGACCACG CTATCTGTGC
            901 AAGGTCCCCG GCCCCGGACG CGCGCTCCAT GAGCAGAGCG CCCGCCGCCG AGGCGAAGAC
            961 TCGGGCGGCG CCCTGCCCGT CCCACCAGGT CAACAGGCGG TAACCGGGCC TCTTCATCGG
           1021 GAATGCGCGC GACCCTTCAG CATCGCCGGC ATGTCCCCTC CTGGCGGGAC GGGGAAGTAT
           1081 CCAGCTCGAC AAGCTTGCGA AGATTTTCAG AGCTAAGCAG CCTAAATGCA GAAAAAATCA
          1141 CTTGCATATA TACCCAACCG G
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user report Shinya E., Quick method of multimeric protein production for biologically active substances such as human GM-CSF (hGM-CSF). Biochem. Biophys. Res. Commun., 386, 40-44 (2009). PMID 19497303.
          user report Yoshida A., Identification of HIV-1 epitopes that induce the synthesis of a R5 HIV-1 suppression factor by human CD4+ T cells isolated from HIV-1 immunized hu-PBL SCID mice. Clin. Dev. Immunol., 12, 235-242 (2005). PMID 16584108.
          user report Yoshida A., Induction of protective immune responses against R5 human immunodeficiency virus type 1 (HIV-1) infection in hu-PBL-SCID mice by intrasplenic immunization with HIV-1-pulsed dendritic cells: possible involvement of a novel factor of human CD4(+) T-cell origin. J. Virol., 77, 8719-8728 (2003). PMID 12885891.
          user report Nagayama Y., A novel murine model of Graves' hyperthyroidism with intramuscular injection of adenovirus expressing the thyrotropin receptor. J. Immunol., 168, 2789-2794 (2002). PMID 11884447.

          2022.02.09

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