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pCM-hGM (#RDB01687)

Human CM-hGM-CSF cDNA

Alternative name CM-hGM-CSF, pCM-hGM
Clone info. Human GM-CSF expression under regulation of CM promoter
Vector backbone pCM-NtTA (Plasmid)
Size of vector backbone 3,875 bp
Selectable markers Amp^r
Gene/insert name Human GM-CSF cDNA
Depositor|Developer Hamada, Hirofumi |
 
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Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link] 
Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Leukoc. Biol., 60, 181-190 (1996).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する(J. Leukoc. Biol., 60, 181-190 (1996).。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01687 pCM-hGM DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pCM-hGM was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01687).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12796_A4Es.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward
Sequence file: RDB12796_A4Esa.seq check
>01687_12796_A4Es_CMV-Foward_C07_07.ab1
    1 TTTTTCCACT TCCGCCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT
  121 TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG CGGCCCCGAA TTCTAGACCA
  181 CCATGTGGCT GCAGAGCCTG CTGCTCTTGG GCACTGTGGC CTGCAGCATC TCTGCACCCG
  241 CCCGCTCGCC CAGCCCCAGC ACGCAGCCCT GGGAGCATGT GAATGCCATC CAGGAGGCCC
  301 GGCGTCTCCT GAACCTGAGT AGAGACACTG CTGCTGAGAT GAATGAAACA GTAGAAGTCA
  361 TCTCAGAAAT GTTTGACCTC CAGGAGCCGA CCTGCCTACA GACCCGCCTG GAGCTGTACA
  421 AGCAGGGCCT GCGGGGCAGC CTCACCAAGC TCAAGGGCCC CTTGACCATG ATGGCCAGCC
  481 ACTACAAGCA GCACTGCCCT CCAACCCCGG AAACTTCCTG TGCAACCCAG ACTATCACCT
  541 TTGAAAGTTT CAAAGAGAAC CTGAAGGACT TTCTGCTTGT CATCCCCTTT GACTGCTGGG
  601 AGCCAGTCCA GGAGTGAGGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC
  661 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
  721 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG
  781 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT
  841 GGTATGGCTG ATTATGATCC TGCAAGCCTC GTCGTCCTGG CCGGACCACG CTATCTGTGC
  901 AAGGTCCCCG GCCCCGGACG CGCGCTCCAT GAGCAGAGCG CCCGCCGCCG AGGCGAAGAC
  961 TCGGGCGGCG CCCTGCCCGT CCCACCAGGT CAACAGGCGG TAACCGGGCC TCTTCATCGG
 1021 GAATGCGCGC GACCCTTCAG CATCGCCGGC ATGTCCCCTC CTGGCGGGAC GGGGAAGTAT
 1081 CCAGCTCGAC AAGCTTGCGA AGATTTTCAG AGCTAAGCAG CCTAAATGCA GAAAAAATCA
1141 CTTGCATATA TACCCAACCG G
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Shinya E., Quick method of multimeric protein production for biologically active substances such as human GM-CSF (hGM-CSF). Biochem. Biophys. Res. Commun., 386, 40-44 (2009). PMID 19497303. [link to RRC of NBRP]
user_report Yoshida A., Identification of HIV-1 epitopes that induce the synthesis of a R5 HIV-1 suppression factor by human CD4+ T cells isolated from HIV-1 immunized hu-PBL SCID mice. Clin. Dev. Immunol., 12, 235-242 (2005). PMID 16584108. [link to RRC of NBRP]
user_report Yoshida A., Induction of protective immune responses against R5 human immunodeficiency virus type 1 (HIV-1) infection in hu-PBL-SCID mice by intrasplenic immunization with HIV-1-pulsed dendritic cells: possible involvement of a novel factor of human CD4(+) T-cell origin. J. Virol., 77, 8719-8728 (2003). PMID 12885891. [link to RRC of NBRP]
user_report Nagayama Y., A novel murine model of Graves' hyperthyroidism with intramuscular injection of adenovirus expressing the thyrotropin receptor. J. Immunol., 168, 2789-2794 (2002). PMID 11884447. [link to RRC of NBRP]