Resource data sheet
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pCM-hGM

Human CM-hGM-CSF cDNA

Catalog number RDB01687
Resource name pCM-hGM
Alternative name CM-hGM-CSF, pCM-hGM
Clone info. Human GM-CSF expression under regulation of CM promoter
Vector backbone pCM-NtTA (Plasmid)
Size of vector backbone 3,875 bp
Selectable markers Amp^r
Gene/insert name Human GM-CSF cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human CSF2 (NCBI Gene 1437) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested.(J. Leukoc. Biol., 60, 181-190 (1996).) In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01687 pCM-hGM DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01687.gif from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12796_A4Es.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: CMV-Forward RDB12796_A4Esa.seq
>01687_12796_A4Es_CMV-Foward_C07_07.ab1
    1 TTTTTCCACT TCCGCCCCCA TTGACGCAAA TGGGCGGTAG GCGTGTACGG TGGGAGGTCT
   61 ATATAAGCAG AGCTCGTTTA GTGAACCGTC AGATCGCCTG GAGACGCCAT CCACGCTGTT
  121 TTGACCTCCA TAGAAGACAC CGGGACCGAT CCAGCCTCCG CGGCCCCGAA TTCTAGACCA
  181 CCATGTGGCT GCAGAGCCTG CTGCTCTTGG GCACTGTGGC CTGCAGCATC TCTGCACCCG
  241 CCCGCTCGCC CAGCCCCAGC ACGCAGCCCT GGGAGCATGT GAATGCCATC CAGGAGGCCC
  301 GGCGTCTCCT GAACCTGAGT AGAGACACTG CTGCTGAGAT GAATGAAACA GTAGAAGTCA
  361 TCTCAGAAAT GTTTGACCTC CAGGAGCCGA CCTGCCTACA GACCCGCCTG GAGCTGTACA
  421 AGCAGGGCCT GCGGGGCAGC CTCACCAAGC TCAAGGGCCC CTTGACCATG ATGGCCAGCC
  481 ACTACAAGCA GCACTGCCCT CCAACCCCGG AAACTTCCTG TGCAACCCAG ACTATCACCT
  541 TTGAAAGTTT CAAAGAGAAC CTGAAGGACT TTCTGCTTGT CATCCCCTTT GACTGCTGGG
  601 AGCCAGTCCA GGAGTGAGGA TCCAGACATG ATAAGATACA TTGATGAGTT TGGACAAACC
  661 ACAACTAGAA TGCAGTGAAA AAAATGCTTT ATTTGTGAAA TTTGTGATGC TATTGCTTTA
  721 TTTGTAACCA TTATAAGCTG CAATAAACAA GTTAACAACA ACAATTGCAT TCATTTTATG
  781 TTTCAGGTTC AGGGGGAGGT GTGGGAGGTT TTTTAAAGCA AGTAAAACCT CTACAAATGT
  841 GGTATGGCTG ATTATGATCC TGCAAGCCTC GTCGTCCTGG CCGGACCACG CTATCTGTGC
  901 AAGGTCCCCG GCCCCGGACG CGCGCTCCAT GAGCAGAGCG CCCGCCGCCG AGGCGAAGAC
  961 TCGGGCGGCG CCCTGCCCGT CCCACCAGGT CAACAGGCGG TAACCGGGCC TCTTCATCGG
 1021 GAATGCGCGC GACCCTTCAG CATCGCCGGC ATGTCCCCTC CTGGCGGGAC GGGGAAGTAT
 1081 CCAGCTCGAC AAGCTTGCGA AGATTTTCAG AGCTAAGCAG CCTAAATGCA GAAAAAATCA
1141 CTTGCATATA TACCCAACCG G
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Shinya E., Quick method of multimeric protein production for biologically active substances such as human GM-CSF (hGM-CSF). Biochem. Biophys. Res. Commun., 386, 40-44 (2009). PMID 19497303.
user report Yoshida A., Identification of HIV-1 epitopes that induce the synthesis of a R5 HIV-1 suppression factor by human CD4+ T cells isolated from HIV-1 immunized hu-PBL SCID mice. Clin. Dev. Immunol., 12, 235-242 (2005). PMID 16584108.
user report Yoshida A., Induction of protective immune responses against R5 human immunodeficiency virus type 1 (HIV-1) infection in hu-PBL-SCID mice by intrasplenic immunization with HIV-1-pulsed dendritic cells: possible involvement of a novel factor of human CD4(+) T-cell origin. J. Virol., 77, 8719-8728 (2003). PMID 12885891.
user report Nagayama Y., A novel murine model of Graves' hyperthyroidism with intramuscular injection of adenovirus expressing the thyrotropin receptor. J. Immunol., 168, 2789-2794 (2002). PMID 11884447.

2018.12.10

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