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pHHO2-1

Human a non-inducible type of heme oxygenase isozyme cDNA

Catalog number RDB01671
Resource name pHHO2-1
Alternative name A non-inducible type of heme oxygenase isozymes
Clone info. Human a non-inducible type of heme oxygenase isozyme cDNA
Vector backbone pcD2-X (Plasmid)
Size of vector backbone 3 kb
Selectable markers Amp^r
Gene/insert name Human Heme oxygenase isozyme cDNA
Reference of insert sequence D21243 (DDBJ accession number)
Depositor|Developer Shibahara, Shigeki |
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External Database
Human Heme oxygenase isozyme

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biochem.113: 214-8, 1993).
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
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          提供条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biochem.113: 214-8, 1993)。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01671 pHHO2-1 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01671.gif

          Original reference

          original Ishikawa, K., Heme oxygenase-2. Properties of the heme complex of the purified tryptic fragment of recombinant human heme oxygenase-2. J. Biol. Chem., 270, 6345-6350 (1995). PMID 7890772.
          original Shibahara, S., Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation. J. Biochem. (Tokyo), 113, 214-218 (1993). PMID 8468327.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13949_A6Atp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: Amp_R
          Sequence file: RDB13949_A6Ata.seq check
          >01671_13949_A6At_2_Amp_R_F07_16.ab1
              1 TTCTTTTTAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
             61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
            121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
            181 GGCCCTTTCG TCTTCAAGAA TTGGTCGACC AATTCTCATG TTTGACAGCT TATCATCGAT
            241 AAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG
            301 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG
            361 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC
            421 CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC
            481 ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT
            541 TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCTCGAGG
            601 AACTGAAAAA CCAGAAAGTT AACTGGTAAG TTTAGTCTTT TTGTCTTTTA TTTCAGGTCC
            661 CGGATCCGGT GGTGGTGCAA ATCAAAGAAC TGCTCCTCAG TGGATGTTGC CTTTACTTCT
            721 AGGCCTGTAC GGAAGTGTTA CTTCTGCTCT AAAAGCTGCT GCAGGGGGGG GGGGGGGGGG
          781 GGG
          //
          Primer: HMOX2-M_R2
          Sequence file: RDB13949_A6Atb.seq check
          >01671_13949_A6At_2_HMOX2-M_R2_A12_03.ab1
              1 CTGCAGCCGA TCAGTTCATT GATATCTGCA TGTTATACTC AAAAGCCTTG TTGGCCTCCT
             61 CCACGATCCT CTCTTTGGTC TTCATGTTCA GGTCCAGGGC GTTCATCCTG GCCCGGTAGA
            121 GCTGCTTGAA CTGCTGGGCA TTGTCCACAT TCTCAAACAG GTAGAACTGG GTCCCTTCCC
            181 CTGTGCTGGG GAGTTTCAGT GCTCGCTGGG CCACCTTCTT CAGCACCTGG CCCCCCGAGA
            241 GATCCCCCAT GTAGCGGGTG TATGCATGGG CCACCAGTAG CTCCGGCTCG TTCTGCCCTA
            301 TGTAGTGGAT CCGCTCCACG TACTTCTGGG CAGCCTTGGG GCACTGCACC TGCTCCTCCC
            361 AGTTTTCACC AAAGAAATAC TCCATGTCCT TGGTCAGCGC CTCCTTCCGG TGCAGCTCCA
            421 TGGGGAAGTA CAAAGGGGCA AAGGCTGGAT GGTCCTTGTT GCGCTCCATT TCCTCCTCGA
            481 GGGCTGAGTA TGTGAAGTAA AGTGCCGTGG TGGCCAGCTT AAACAGCTCC TTCTTAATGT
            541 TGCCTTTCAA GAAGTCCTTG ACAAACTGGG TGTTTTCTGC CCGGTCGTGT GCTTCCTTGG
            601 TCCCTTCCTT CAGGAGCTCC GAGAGGTCAG CCATTCTCAT TTGGTTCTCC TTTTCTAGGG
            661 CCCCAGAGTT CTTTTTTTCT GACTCGTCTA CCCCCTCTGA GGTTTCCACT TCCGCTGACA
            721 TTGCTGCTGG GTGTGGTTCT TGCTGCTCTC GCTCCTCTGG TCCTGCCGCA GGTCGCTGTC
            781 GCCTGTCCGC CAGCCTCCAG TCAGCGTGCC CGTCAGCGAC TCCCTCAGGC GCCGCAGTCC
            841 CTGCAGCCCA GCGGACTGGC GCCCCCCCCC CCCTACCCTC AAAAGAGTTT ATGGTGAGAG
          901 TAGCATTGTC TGATAGTCGG AGATAGATGA CGATACA
          //
          Primer: HMOX2-M_F
          Sequence file: RDB13949_A6Atc.seq check
          >01671_13949_A6At_2_HMOX2-M_F_D07_10.ab1
              1 GCGGGGGATG ACTGAGACCA AAGAGAGGAT CGTGGAGGAG GCCAACAAGG CTTTTGAGTA
             61 TAACATGCAG ATATTCAATG AACTGGACCA GGCCGGCTCC ACACTGGCCA GAGAGACCTT
            121 GGAGGATGGG TTCCCTGTAC ACGATGGGAA AGGAGACATG CGTAAATGCC CTTTCTACGC
            181 TGCTGAACAA GACAAAGGTG CCCTGGAGGG CAGCAGCTGT CCCTTCCGAA CAGCTATGGC
            241 TGTGCTGAGG AAGCCCAGCC TCCAGTTCAT CCTGGCCGCT GGTGTGGCCC TAGCTGCTGG
            301 ACTCTTGGCC TGGTACTACA TGTGAAGCAC CCATCATGCC ACACCGGTAC CCTCCTCCCG
            361 ACTGACCACT GGCCTACCCC TTTCTCCAGC CCTGACTAAA CTACCACCTC AGGTGACTTT
            421 TTAAAAAATG CTGGGTTTAA GAAAGGCAAC CAATAAAAGC CAGATGCTAG AAAAAAAAAA
            481 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAGTACCTTC TGAGGCGGAA
            541 AGAACCAGCC GGATCCCTCG AGGGATCCAA ACATGATAAG ATACATTGAT GAGTTTGGAC
            601 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG
            661 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT
            721 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA
            781 AATGTGGTAT GGCTGATTAT GATCCGGCTG CCTCGCGCGT TTCGGTGATG ACGGGTGAAA
            841 ACCTCTGACA CATGCAGCTC CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG
            901 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTGGCGG GTGTCGGGGC GCAGCCATGA
            961 CCAGTCACGT AGCGATAGCG AAGTGTATAC TGCTACTATG CGGCATCGAG CAGATGTACT
          1021 GAAGTGCACA TATGCGGTTG CATACGTCGA TGCTAGAGCA ATACG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ishikawa, K., Heme oxygenase-2. Properties of the heme complex of the purified tryptic fragment of recombinant human heme oxygenase-2. J. Biol. Chem., 270, 6345-6350 (1995). PMID 7890772.
          original Shibahara, S., Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation. J. Biochem. (Tokyo), 113, 214-218 (1993). PMID 8468327.

          2022.02.09

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