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pHHO2-1 (#RDB01671)

Human a non-inducible type of heme oxygenase isozyme cDNA

Alternative name A non-inducible type of heme oxygenase isozymes
Clone info. Human a non-inducible type of heme oxygenase isozyme cDNA
Vector backbone pcD2-X (Plasmid)
Size of vector backbone 3 kb
Selectable markers Amp^r
Gene/insert name Human Heme oxygenase isozyme cDNA
Reference of insert sequence D21243 (DDBJ accession number)
Depositor|Developer Shibahara, Shigeki |
 
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Distribution information

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MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
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Terms and conditions for distribution In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested (J. Biochem.113: 214-8, 1993).
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する (J. Biochem.113: 214-8, 1993)。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01671 pHHO2-1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHHO2-1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01671).

Reference section:

Ishikawa, K., Takeuchi, N., Takahashi, S., Matera, K.M., Sato, M., Shibahara, S., Rousseau, D.L., Ikeda-Saito, M., Yoshida, T., Heme oxygenase-2. Properties of the heme complex of the purified tryptic fragment of recombinant human heme oxygenase-2. J. Biol. Chem., 270, 6345-6350 (1995). PMID 7890772. [link to RRC of NBRP]
Shibahara, S., Yoshizawa, M., Suzuki, H., Takeda, K., Meguro, K., Endo, K., Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation. J. Biochem. (Tokyo), 113, 214-218 (1993). PMID 8468327. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB13949_A6Atp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R
Sequence file: RDB13949_A6Ata.seq check
>01671_13949_A6At_2_Amp_R_F07_16.ab1
    1 TTCTTTTTAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
  181 GGCCCTTTCG TCTTCAAGAA TTGGTCGACC AATTCTCATG TTTGACAGCT TATCATCGAT
  241 AAGCTTGGCT GTGGAATGTG TGTCAGTTAG GGTGTGGAAA GTCCCCAGGC TCCCCAGCAG
  301 GCAGAAGTAT GCAAAGCATG CATCTCAATT AGTCAGCAAC CAGGTGTGGA AAGTCCCCAG
  361 GCTCCCCAGC AGGCAGAAGT ATGCAAAGCA TGCATCTCAA TTAGTCAGCA ACCATAGTCC
  421 CGCCCCTAAC TCCGCCCATC CCGCCCCTAA CTCCGCCCAG TTCCGCCCAT TCTCCGCCCC
  481 ATGGCTGACT AATTTTTTTT ATTTATGCAG AGGCCGAGGC CGCCTCGGCC TCTGAGCTAT
  541 TCCAGAAGTA GTGAGGAGGC TTTTTTGGAG GCCTAGGCTT TTGCAAAAAG CTCCTCGAGG
  601 AACTGAAAAA CCAGAAAGTT AACTGGTAAG TTTAGTCTTT TTGTCTTTTA TTTCAGGTCC
  661 CGGATCCGGT GGTGGTGCAA ATCAAAGAAC TGCTCCTCAG TGGATGTTGC CTTTACTTCT
  721 AGGCCTGTAC GGAAGTGTTA CTTCTGCTCT AAAAGCTGCT GCAGGGGGGG GGGGGGGGGG
781 GGG
//
Primer: HMOX2-M_R2
Sequence file: RDB13949_A6Atb.seq check
>01671_13949_A6At_2_HMOX2-M_R2_A12_03.ab1
    1 CTGCAGCCGA TCAGTTCATT GATATCTGCA TGTTATACTC AAAAGCCTTG TTGGCCTCCT
   61 CCACGATCCT CTCTTTGGTC TTCATGTTCA GGTCCAGGGC GTTCATCCTG GCCCGGTAGA
  121 GCTGCTTGAA CTGCTGGGCA TTGTCCACAT TCTCAAACAG GTAGAACTGG GTCCCTTCCC
  181 CTGTGCTGGG GAGTTTCAGT GCTCGCTGGG CCACCTTCTT CAGCACCTGG CCCCCCGAGA
  241 GATCCCCCAT GTAGCGGGTG TATGCATGGG CCACCAGTAG CTCCGGCTCG TTCTGCCCTA
  301 TGTAGTGGAT CCGCTCCACG TACTTCTGGG CAGCCTTGGG GCACTGCACC TGCTCCTCCC
  361 AGTTTTCACC AAAGAAATAC TCCATGTCCT TGGTCAGCGC CTCCTTCCGG TGCAGCTCCA
  421 TGGGGAAGTA CAAAGGGGCA AAGGCTGGAT GGTCCTTGTT GCGCTCCATT TCCTCCTCGA
  481 GGGCTGAGTA TGTGAAGTAA AGTGCCGTGG TGGCCAGCTT AAACAGCTCC TTCTTAATGT
  541 TGCCTTTCAA GAAGTCCTTG ACAAACTGGG TGTTTTCTGC CCGGTCGTGT GCTTCCTTGG
  601 TCCCTTCCTT CAGGAGCTCC GAGAGGTCAG CCATTCTCAT TTGGTTCTCC TTTTCTAGGG
  661 CCCCAGAGTT CTTTTTTTCT GACTCGTCTA CCCCCTCTGA GGTTTCCACT TCCGCTGACA
  721 TTGCTGCTGG GTGTGGTTCT TGCTGCTCTC GCTCCTCTGG TCCTGCCGCA GGTCGCTGTC
  781 GCCTGTCCGC CAGCCTCCAG TCAGCGTGCC CGTCAGCGAC TCCCTCAGGC GCCGCAGTCC
  841 CTGCAGCCCA GCGGACTGGC GCCCCCCCCC CCCTACCCTC AAAAGAGTTT ATGGTGAGAG
901 TAGCATTGTC TGATAGTCGG AGATAGATGA CGATACA
//
Primer: HMOX2-M_F
Sequence file: RDB13949_A6Atc.seq check
>01671_13949_A6At_2_HMOX2-M_F_D07_10.ab1
    1 GCGGGGGATG ACTGAGACCA AAGAGAGGAT CGTGGAGGAG GCCAACAAGG CTTTTGAGTA
   61 TAACATGCAG ATATTCAATG AACTGGACCA GGCCGGCTCC ACACTGGCCA GAGAGACCTT
  121 GGAGGATGGG TTCCCTGTAC ACGATGGGAA AGGAGACATG CGTAAATGCC CTTTCTACGC
  181 TGCTGAACAA GACAAAGGTG CCCTGGAGGG CAGCAGCTGT CCCTTCCGAA CAGCTATGGC
  241 TGTGCTGAGG AAGCCCAGCC TCCAGTTCAT CCTGGCCGCT GGTGTGGCCC TAGCTGCTGG
  301 ACTCTTGGCC TGGTACTACA TGTGAAGCAC CCATCATGCC ACACCGGTAC CCTCCTCCCG
  361 ACTGACCACT GGCCTACCCC TTTCTCCAGC CCTGACTAAA CTACCACCTC AGGTGACTTT
  421 TTAAAAAATG CTGGGTTTAA GAAAGGCAAC CAATAAAAGC CAGATGCTAG AAAAAAAAAA
  481 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAGTACCTTC TGAGGCGGAA
  541 AGAACCAGCC GGATCCCTCG AGGGATCCAA ACATGATAAG ATACATTGAT GAGTTTGGAC
  601 AAACCACAAC TAGAATGCAG TGAAAAAAAT GCTTTATTTG TGAAATTTGT GATGCTATTG
  661 CTTTATTTGT AACCATTATA AGCTGCAATA AACAAGTTAA CAACAACAAT TGCATTCATT
  721 TTATGTTTCA GGTTCAGGGG GAGGTGTGGG AGGTTTTTTA AAGCAAGTAA AACCTCTACA
  781 AATGTGGTAT GGCTGATTAT GATCCGGCTG CCTCGCGCGT TTCGGTGATG ACGGGTGAAA
  841 ACCTCTGACA CATGCAGCTC CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG
  901 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTGGCGG GTGTCGGGGC GCAGCCATGA
  961 CCAGTCACGT AGCGATAGCG AAGTGTATAC TGCTACTATG CGGCATCGAG CAGATGTACT
1021 GAAGTGCACA TATGCGGTTG CATACGTCGA TGCTAGAGCA ATACG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishikawa, K., Heme oxygenase-2. Properties of the heme complex of the purified tryptic fragment of recombinant human heme oxygenase-2. J. Biol. Chem., 270, 6345-6350 (1995). PMID 7890772. [link to RRC of NBRP]
original Shibahara, S., Functional analysis of cDNAs for two types of human heme oxygenase and evidence for their separate regulation. J. Biochem. (Tokyo), 113, 214-218 (1993). PMID 8468327. [link to RRC of NBRP]