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pHM31 (#RDB01652)

Human mu-calpain large subunit cDNA

Alternative name Human mu-calpain large subunit
Clone info. Catalytic subunit of human Ca^2+-dependent intracellular cysteine proteinase, micro-calpain
Vector backbone pUC19 (Plasmid)
Size of vector backbone 2.7 kb
Selectable markers Amp^r
Gene/insert name Human mu-calpain cDNA
Depositor|Developer Sorimachi, Hiroyuki |
 
Sequence (full) RDB01652zzt01.seq compiled by us
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01652 pHM31 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHM31 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01652).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB01652_B2D7p1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F (Pr0536)
Region: insert 5'(5' UTR,CDS)
Sequence file: RDB01652_B2D7a.seq check
>01652_01652_B2D7_2_pUC-CAPbs_up_F_B01_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGNNNNNN NNCAATTAAT GTGAGTTAGC TCACTCATTA
   61 GGCACCCCAG GCTTTACACT TTATGCTTCC GGCTCGTATG TTGTGTGGAA TTGTGAGCGG
  121 ATAACAATTT CACACAGGAA ACAGCTATGA CCATGATTAC GCCAAGCTTG CATGCCTGCA
  181 GGTCGACTCT AGAGGATCCC CGGGTACCGA GCTCGAATTC CGGGCCTTTT ATTATTTGGG
  241 GTTCCTCTAT ATGGAACACG GGACCTCCCC ACCCCAAGCC TAGTGCCTCT GGCACCCCCA
  301 TAACCAGTAC ACAAGTCCCT GAAGAGTCCC CTTTAAAATC ACTAATATAA AAAAGGAGCG
  361 AAGGAGGGAA GGCCGACCGC CCACAGGCTG GGCAGCTCCT GGTCGGCAGC AGGGCCAGGG
  421 AAGGACAGAG GGGGCGTTGG CTTCGGCGCA GGAAGGCACA GGCGTTCCCA GCCCCGGGGC
  481 CTCCCCGAGC CGGGAGGCGG CTCCACACGC CTGGACTGCA GACTGTGTCG AGCTAGTGGT
  541 TATAGTTTAT AGTCTGCAGG CAAGGCCAGG CCGGTGGTGC TGAGTGGGTG GCCTCTGAAG
  601 TGGGGAGAGG GGACACATCA GGATGCTGGG GAAACCCGGG GCTGTCCCGA GGCAGGAACA
  661 AGCGAAAGCT GCCTTCCTGG CTTGGCAATG GGTGGGGCCC AGGGAGTCCA TCCCGAGCCC
  721 ATGGCTGGCA CAGGCAGGGG CCACACAGTT CTGCCCGGAG CAGATGAACG ATGGTGGCTG
  781 GGANGAGAGG GGACAGGANG ANGANGCTGC CTCTCTGCTC CNAGGAANGC ACTTGCAGCT
  841 GGGGTGGNCT GNTGTGGTGT GGTANGAGGN GANGCTTGNC ANANNANGAN GGANGGGANC
  901 ACGGNAANGG GGANCNANTC CCTGNNTCAT GNNAACATGG NCAGCTGCAA CCACTTAAAC
  961 NAGNCNAAGG TCNNNACTCN NTCCNNATCT GTGTNNNNAN TTTTGAAAAA TCGNNNNNNN
1021 GNNCNCNANN CNNCNNNNNN NNNCAAACNA ANNNNNNNAN NNNNNNNNN
//
Primer: pUC-M13_-100 (Pr0310)
Region: insert 3' (3' UTR)
Sequence file: RDB01652_B2D7b.seq check
>01652_01652_B2D7_2_pUC-M13_-100_B03_06_ABI24.ab1
    1 NNNNNNNNNN NNGNANGCNN GGGTTTTCCN NGTCNCGACG TTGTAAAACG ACGGCCAGTG
   61 AATTCCGGGG GGGGGAAGGA GAGAGGGAGG GCGGAGGGCG GAGGGGCGGC GGGAGGAGGG
  121 CGGGGAGGAG CGCTCTTCCT GGTTGGGCCC TGCCCTGAGC TGCCACCGGG AAGCCAGCCT
  181 CAGGGACTGC AGCGACCCCC AAACACCCCT CCCCCAGGAT GTCGGAGGAG ATCATCACGC
  241 CGGTGTACTG CACTGGGGTG TCAGCCCAAG TGCAGAAGCA GCGGGCCAGG GAGCTGGGCC
  301 TGGGCCGCCA TGAGAATGCC ATCAAGTACC TGGGCCAGGA TTATGAGCAG CTGCGGGTGC
  361 GATGCCTGCA NAGTGNGACC NTCNTCCGNG ANGANNNCTT NCCCCCCGNN NNNNNNNANN
421 NNNNGTTACN NNGNNNNGGN NNNNNNNNN
//
Primer: HM31-B_R (Pr1193)
Region: insert mid(3' UTR,CDS)
Sequence file: RDB01652_B2D7c.seq check
>01652_01652_B2D7_2_HM31-B_R_B02_02_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNGNN GNGNNGCTTG GCAGANGAGG AGGGAGGGGA GCACGGCAAG
   61 GGGGACCGAG TCCCTGCCTC ATGCAAACAT GGTCAGCTGC AACCACTTAA ACAAGTCAAA
  121 GGTCACAACT CCATCCAGAT CTGTGTCCAG AGTTTTGAAA AATCGGAACA TGGTCTCTAG
  181 CCGCACCAGG CAGCAAACGA AATTGTCAAA GTCGACCGCC AGGTCGGGCT CCGAGTAGCG
  241 GGTGATGATG AGCTCGTACA GCTTCTTGTT GAGCTTGAAG CCTGCCGACT CAATGGCCAT
  301 CCGCATCTCG TAGGCACTCA TGCTGCCCGA CTTGTCCAGG TCAAACTTCC GGAAGATGGA
  361 CAGGTAATTC CGGATGCGGT TCCACAGGAT GTTGAACTCC ACCAGGCCCA GCTTCCCATT
  421 GCCATCACGA TCCATGAGGT TCACCATGCT GCGGCACGAC TCTAGGCTGA AGCCCTTGGT
  481 CCGCAGGTCT TTGTGTTTGC TGATGATCCT ATTGAGGATT GTCCGCAACT CCTTCACGCT
  541 GATCTCCATG TCCTCCCCTG CCAGCTGCCT GAAGAGGGCC TTGAAGTTCT CGTCAATCTC
  601 CTCTTCTGAG AGCACTTGCT CATCGGGGAN ANTGGCCTGN ANCTGGTCNT CCNGCTCCNC
  661 AGTCCCNGCA CTCTTCTCNG ANAAGAANCN CANCACGAAN TCNCCCNNNN NNNNNGGNTC
721 NNNNNNNNNG NNNNNNNNCN TNNNNCNNNN NNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

reference Ohno, S., Four genes for the calpain family locate on four distinct human chromosomes. Cytogenet. Cell Genet., 53, 225-229 (1990). PMID 2209092.
reference Aoki, K., Complete amino acid sequence of the large subunit of the low-Ca2+-requiring form of human Ca2+-activated neutral protease (muCANP) deduced from its cDNA sequence. FEBS Lett., 205, 313-317 (1986). PMID 3017764.