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pBluescript SKII+hTNF alpha

Human TNF-alpha cDNA

Catalog number RDB01477
Resource name pBluescript SKII+hTNF alpha
Alternative name Human TNF alpha cDNA
Clone info. Human TNF alpha cDNA
Vector backbone pBluescript II SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human TNF-alpha cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank human TNF (NCBI Gene 7124) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01477 pBluescript SKII+hTNF alpha DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01477.gif from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB14264_A6Gjp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40 RDB14264_A6Gja.seq
>01477_14264_A6Gj_M13(-40)_A01_01.ab1
    1 CCGGCCTGTG CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGGTACCGG GCCCCCCCTC
   61 GAGGTCGACG GTATCGATAA GCTTGATATC GAATTCTAGA CACCATGAGC ACTGAAAGCA
  121 TGATCCGGGA CGTGGAGCTG GCCGAGGAGG CGCTCCCCAA GAAGACAGGG GGGCCCCAGG
  181 GCTCCAGGCG GTGCTTGTTC CTCAGCCTCT TCTCCTTCCT GATCGTGGCA GGCGCCACCA
  241 CGCTCTTCTG CCTGCTGCAC TTTGGAGTGA TCGGCCCCCA GAGGGAAGAG TTCCCCAGGG
  301 ACCTCTCTCT AATCAGCCCT CTGGCCCAGG CAGTCAGATC ATCTTCTCGA ACCCCGAGTG
  361 ACAAGCCTGT AGCCCATGTT GTAGCAAACC CTCAAGCTGA GGGGCAGCTC CAGTGGCTGA
  421 ACCGCCGGGC CAATGCCCTC CTGGCCAATG GCGTGGAGCT GAGAGATAAC CAGCTGGTGG
  481 TGCCATCAGA GGGCCTGTAC CTCATCTACT CCCAGGTCCT CTTCAAGGGC CAAGGCTGCC
  541 CCTCCACCCA TGTGCTCCTC ACCCACACCA TCAGCCGCAT CGCCGTCTCC TACCAGACCA
  601 AGGTCAACCT CCTCTCTGCC ATCAAGAGCC CCTGCCAGAG GGAGACCCCA GAGGGGGCTG
  661 AGGCCAAGCC CTGGTATGAG CCCATCTATC TGGGAGGGGT CTTCCAGCTG GAGAAGGGTG
  721 ACCGACTCAG CGCTGAGATC AATCGGCCCG ACTATCTCGA CTTTGCCGAG TCTGGGCAGG
  781 TCTACTTTGG GATCATTGCC CTGTGACTCG AGGATCCACT AGTTCTAGAG CGGCCGCCAC
  841 CGCGGTGGAG CTCCAGCTTT TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT
  901 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTTATCCGC TCACAAATTC CACACAACAT
  961 ACGAGCCCGG GAAGCATAAA GTGTAAAGCC TGGGGTGCCC TAAATGAGTG AGCTACTCAC
 1021 ATTTAACTGC CGATGCGCTC ACTGGCCCGC TTTCCAGTCA GGCAACCTGA TCGTTGCCAG
1081 CTGTGGCCAA TA
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Yokoyama, C., Interleukin-8 enhances the effect of colchicine on cell death. Biochem. Biophys. Res. Commun. 485 (1): 89-94 (2017). PMID 28189686.

2018.12.11

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