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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBluescript SKII+hTNF alpha (#RDB01477)

Human TNF-alpha cDNA

Alternative name Human TNF alpha cDNA
Clone info. Human TNF alpha cDNA
Vector backbone pBluescript II SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human TNF-alpha cDNA
Depositor|Developer Hamada, Hirofumi |
 
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Distribution information

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Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
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提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
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MTAに書く使用条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01477 pBluescript SKII+hTNF alpha DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pBluescript SKII+hTNF alpha was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01477).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14264_A6Gjp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB14264_A6Gja.seq check
>01477_14264_A6Gj_M13(-40)_A01_01.ab1
    1 CCGGCCTGTG CGCGCGTATA CGACTCACTA TAGGGCGAAT TGGGTACCGG GCCCCCCCTC
   61 GAGGTCGACG GTATCGATAA GCTTGATATC GAATTCTAGA CACCATGAGC ACTGAAAGCA
  121 TGATCCGGGA CGTGGAGCTG GCCGAGGAGG CGCTCCCCAA GAAGACAGGG GGGCCCCAGG
  181 GCTCCAGGCG GTGCTTGTTC CTCAGCCTCT TCTCCTTCCT GATCGTGGCA GGCGCCACCA
  241 CGCTCTTCTG CCTGCTGCAC TTTGGAGTGA TCGGCCCCCA GAGGGAAGAG TTCCCCAGGG
  301 ACCTCTCTCT AATCAGCCCT CTGGCCCAGG CAGTCAGATC ATCTTCTCGA ACCCCGAGTG
  361 ACAAGCCTGT AGCCCATGTT GTAGCAAACC CTCAAGCTGA GGGGCAGCTC CAGTGGCTGA
  421 ACCGCCGGGC CAATGCCCTC CTGGCCAATG GCGTGGAGCT GAGAGATAAC CAGCTGGTGG
  481 TGCCATCAGA GGGCCTGTAC CTCATCTACT CCCAGGTCCT CTTCAAGGGC CAAGGCTGCC
  541 CCTCCACCCA TGTGCTCCTC ACCCACACCA TCAGCCGCAT CGCCGTCTCC TACCAGACCA
  601 AGGTCAACCT CCTCTCTGCC ATCAAGAGCC CCTGCCAGAG GGAGACCCCA GAGGGGGCTG
  661 AGGCCAAGCC CTGGTATGAG CCCATCTATC TGGGAGGGGT CTTCCAGCTG GAGAAGGGTG
  721 ACCGACTCAG CGCTGAGATC AATCGGCCCG ACTATCTCGA CTTTGCCGAG TCTGGGCAGG
  781 TCTACTTTGG GATCATTGCC CTGTGACTCG AGGATCCACT AGTTCTAGAG CGGCCGCCAC
  841 CGCGGTGGAG CTCCAGCTTT TGTTCCCTTT AGTGAGGGTT AATTGCGCGC TTGGCGTAAT
  901 CATGGTCATA GCTGTTTCCT GTGTGAAATT GTTTATCCGC TCACAAATTC CACACAACAT
  961 ACGAGCCCGG GAAGCATAAA GTGTAAAGCC TGGGGTGCCC TAAATGAGTG AGCTACTCAC
 1021 ATTTAACTGC CGATGCGCTC ACTGGCCCGC TTTCCAGTCA GGCAACCTGA TCGTTGCCAG
1081 CTGTGGCCAA TA
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

user_report Yokoyama, C., Interleukin-8 enhances the effect of colchicine on cell death. Biochem. Biophys. Res. Commun. 485 (1): 89-94 (2017). PMID 28189686. [link to RRC of NBRP]