Resource data sheet
Prev.
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pBluescript SKII+mGM-CSF

Mouse GM-CSF cDNA

Catalog number RDB01469
Resource name pBluescript SKII+mGM-CSF
Alternative name Mouse GM-CSF cDNA
Clone info. Mouse GM-CSF cDNA
Vector backbone pBluescript II SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse GM-CSF cDNA
Depositor|Developer Hamada, Hirofumi |
Other clones in our bank

External Database
Mouse GM-CSF

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
          Ordering Please visit Information of Request for Distribution.[link] 
          Order form 
          [Credit Card Payment]  [Bank Transfer Payment]
          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。
          提供依頼 手続きの詳細は、「提供申込みについて[link]」をご覧ください。
          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01469 pBluescript SKII+mGM-CSF DNA solution Please contact us.

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01469.gif

          Original reference

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB12797_A4Es.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: T7_20bp
          Sequence file: RDB12797_A4Esa.seq check
          >01469_12797_A4Es_T7_20bp_A07_01.ab1
              1 TGCTTAGGAC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTAGACACCA
             61 TGTGGCTGCA GAATTTACTT TTCCTGGGCA TTGTGGTCTA CAGCCTCTCA GCACCCACCC
            121 GCTCACCCAT CACTGTCACC CGGCCTTGGA AGCATGTAGA GGCCATCAAA GAAGCCCTGA
            181 ACCTCCTGGA TGACATGCCT GTCACGTTGA ATGAAGAGGT AGAAGTCGTC TCTAACGAGT
            241 TCTCCTTCAA GAAGCTAACA TGTGTGCAGA CCCGCCTGAA GATATTCGAG CAGGGTCTAC
            301 GGGGCAATTT CACCAAACTC AAGGGCGCCT TGAACATGAC AGCCAGCTAC TACCAGACAT
            361 ACTGCCCCCC AACTCCGGAA ACGGACTGTG AAACACAAGT TACCACCTAT GCGGATTTCA
            421 TAGACAGCCT TAAAACCTTT CTGACTGATA TCCCCTTTGA ATGCAAAAAA CCAAGCCAAA
            481 AATGAGGATC CACTAGTTCT AGAGCGGCCG CCACCGCGGT GGAGCTCCAG CTTTTGTTCC
            541 CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA
            601 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC
            661 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
            721 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
            781 GGTTTGCGTA TTGGGCGCTC TTCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGTCGTTCG
            841 GCTGGCGGCG AGCGTATTCA GCTCACTCAA GCGTAATACG TTATCCCACA GAATCAGGAT
            901 ACGCAGAAAG AACATGGTGG AGCAAAGGCA GCCAAGCAGA TCGGTAAGGC CCGTGCTGCC
            961 ATTTCTAGTC GGCCCCTGAC CAGACTCCAT TCCGACGCTC AAGTCAAGGT GGCACCGACG
          1021 ACAAGGATTC CGGCCTCTGA TCCTGGGACT CCAGTCC
          //
          Primer: Reverse2
          Sequence file: RDB12797_A4Esb.seq check
          >01469_12797_A4Es_Reverse2_A07_01.ab1
              1 CGGAAAATGA TTTACGCCAG CGCGCAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC
             61 TCCACCGCGG TGGCGGCCGC TCTAGAACTA GTGGATCCTC ATTTTTGGCT TGGTTTTTTG
            121 CATTCAAAGG GGATATCAGT CAGAAAGGTT TTAAGGCTGT CTATGAAATC CGCATAGGTG
            181 GTAACTTGTG TTTCACAGTC CGTTTCCGGA GTTGGGGGGC AGTATGTCTG GTAGTAGCTG
            241 GCTGTCATGT TCAAGGCGCC CTTGAGTTTG GTGAAATTGC CCCGTAGACC CTGCTCGAAT
            301 ATCTTCAGGC GGGTCTGCAC ACATGTTAGC TTCTTGAAGG AGAACTCGTT AGAGACGACT
            361 TCTACCTCTT CATTCAACGT GACAGGCATG TCATCCAGGA GGTTCAGGGC TTCTTTGATG
            421 GCCTCTACAT GCTTCCAAGG CCGGGTGACA GTGATGGGTG AGCGGGTGGG TGCTGAGAGG
            481 CTGTAGACCA CAATGCCCAG GAAAAGTAAA TTCTGCAGCC ACATGGTGTC TAGAATTCGA
            541 TATCAAGCTT ATCGATACCG TCGACCTCGA GGGGGGGCCC GGTACCCAAT TCGCCCTATA
            601 GTGAGTCGTA TTACGCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC
            661 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA
            721 GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG
            781 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG
            841 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA
            901 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGGC TCCCTTTAGG GTTCCGATTT
            961 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTTGATTAG GGTGATGGTT CACGTAGTGG
           1021 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTT GGGAGTCCCA CGTTCTTTAT
           1081 TAGTGAACTC TTGATCCCAA ACTGACCACA CTCAACCTAT CTCGATCTAA TTCATTTGGA
          1141 TTTTTACCAC AGGCGGCA
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          user report Kinoshita Y., Antitumor Effect on Murine Renal Cell Carcinoma by Autologous Tumor Vaccines Genetically Modified with Granulocyte-Macrophage Colony-Stimulating Factor and Interleukin-6 Cells. J. Immunother (1991)., 24, 205-211 (2001). PMID 11395635.
          user report Nishisaka N., Immunotherapy for lung metastases of murine renal cell carcinoma: synergy between radiation and cytokine-producing tumor vaccines. J. Immunother., 22, 308-314 (1999). PMID 10404432.
          user report Sakai T., DNA immunization with Plasmodium falciparum serine repeat antigen: regulation of humoral immune response by coinoculation of cytokine expression plasmid. Parasitol. Int., 48, 27-33 (1999). PMID 11269323.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl