Resource data sheet
Prev.

pBluescript SKII+mGM-CSF

Mouse GM-CSF cDNA

Catalog number RDB01469
Resource name pBluescript SKII+mGM-CSF
Alternative name Mouse GM-CSF cDNA
Clone info. Mouse GM-CSF cDNA
Vector backbone pBluescript II SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse GM-CSF cDNA
Depositor Hamada, Hirofumi |
 
Other clones in our bank mouse Csf2 (NCBI Gene 12981) |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Ordering Please visit Information of Request for Distribution. 
Order form 
[Credit Card Payment]  [Bank Transfer or Check Payment]
Material Transfer Agreement (MTA) [Word]
Distribution information (domestic users) [open/close]


Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01469 pBluescript SKII+mGM-CSF DNA solution Please contact us.

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB01469.gif from Depositor

Featured content

References

User reports and related articles (go to bottom)


Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB12797_A4Es.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: T7_20bp RDB12797_A4Esa.seq
>01469_12797_A4Es_T7_20bp_A07_01.ab1
    1 TGCTTAGGAC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CTAGACACCA
   61 TGTGGCTGCA GAATTTACTT TTCCTGGGCA TTGTGGTCTA CAGCCTCTCA GCACCCACCC
  121 GCTCACCCAT CACTGTCACC CGGCCTTGGA AGCATGTAGA GGCCATCAAA GAAGCCCTGA
  181 ACCTCCTGGA TGACATGCCT GTCACGTTGA ATGAAGAGGT AGAAGTCGTC TCTAACGAGT
  241 TCTCCTTCAA GAAGCTAACA TGTGTGCAGA CCCGCCTGAA GATATTCGAG CAGGGTCTAC
  301 GGGGCAATTT CACCAAACTC AAGGGCGCCT TGAACATGAC AGCCAGCTAC TACCAGACAT
  361 ACTGCCCCCC AACTCCGGAA ACGGACTGTG AAACACAAGT TACCACCTAT GCGGATTTCA
  421 TAGACAGCCT TAAAACCTTT CTGACTGATA TCCCCTTTGA ATGCAAAAAA CCAAGCCAAA
  481 AATGAGGATC CACTAGTTCT AGAGCGGCCG CCACCGCGGT GGAGCTCCAG CTTTTGTTCC
  541 CTTTAGTGAG GGTTAATTGC GCGCTTGGCG TAATCATGGT CATAGCTGTT TCCTGTGTGA
  601 AATTGTTATC CGCTCACAAT TCCACACAAC ATACGAGCCG GAAGCATAAA GTGTAAAGCC
  661 TGGGGTGCCT AATGAGTGAG CTAACTCACA TTAATTGCGT TGCGCTCACT GCCCGCTTTC
  721 CAGTCGGGAA ACCTGTCGTG CCAGCTGCAT TAATGAATCG GCCAACGCGC GGGGAGAGGC
  781 GGTTTGCGTA TTGGGCGCTC TTCGCTTCCT CGCTCACTGA CTCGCTGCGC TCGTCGTTCG
  841 GCTGGCGGCG AGCGTATTCA GCTCACTCAA GCGTAATACG TTATCCCACA GAATCAGGAT
  901 ACGCAGAAAG AACATGGTGG AGCAAAGGCA GCCAAGCAGA TCGGTAAGGC CCGTGCTGCC
  961 ATTTCTAGTC GGCCCCTGAC CAGACTCCAT TCCGACGCTC AAGTCAAGGT GGCACCGACG
1021 ACAAGGATTC CGGCCTCTGA TCCTGGGACT CCAGTCC
//
Primer: Reverse2 RDB12797_A4Esb.seq
>01469_12797_A4Es_Reverse2_A07_01.ab1
    1 CGGAAAATGA TTTACGCCAG CGCGCAATTA ACCCTCACTA AAGGGAACAA AAGCTGGAGC
   61 TCCACCGCGG TGGCGGCCGC TCTAGAACTA GTGGATCCTC ATTTTTGGCT TGGTTTTTTG
  121 CATTCAAAGG GGATATCAGT CAGAAAGGTT TTAAGGCTGT CTATGAAATC CGCATAGGTG
  181 GTAACTTGTG TTTCACAGTC CGTTTCCGGA GTTGGGGGGC AGTATGTCTG GTAGTAGCTG
  241 GCTGTCATGT TCAAGGCGCC CTTGAGTTTG GTGAAATTGC CCCGTAGACC CTGCTCGAAT
  301 ATCTTCAGGC GGGTCTGCAC ACATGTTAGC TTCTTGAAGG AGAACTCGTT AGAGACGACT
  361 TCTACCTCTT CATTCAACGT GACAGGCATG TCATCCAGGA GGTTCAGGGC TTCTTTGATG
  421 GCCTCTACAT GCTTCCAAGG CCGGGTGACA GTGATGGGTG AGCGGGTGGG TGCTGAGAGG
  481 CTGTAGACCA CAATGCCCAG GAAAAGTAAA TTCTGCAGCC ACATGGTGTC TAGAATTCGA
  541 TATCAAGCTT ATCGATACCG TCGACCTCGA GGGGGGGCCC GGTACCCAAT TCGCCCTATA
  601 GTGAGTCGTA TTACGCGCGC TCACTGGCCG TCGTTTTACA ACGTCGTGAC TGGGAAAACC
  661 CTGGCGTTAC CCAACTTAAT CGCCTTGCAG CACATCCCCC TTTCGCCAGC TGGCGTAATA
  721 GCGAAGAGGC CCGCACCGAT CGCCCTTCCC AACAGTTGCG CAGCCTGAAT GGCGAATGGG
  781 ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG
  841 CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA
  901 CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGGC TCCCTTTAGG GTTCCGATTT
  961 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTTGATTAG GGTGATGGTT CACGTAGTGG
 1021 GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTT GGGAGTCCCA CGTTCTTTAT
 1081 TAGTGAACTC TTGATCCCAA ACTGACCACA CTCAACCTAT CTCGATCTAA TTCATTTGGA
1141 TTTTTACCAC AGGCGGCA
//

Please visit Sequencing and PCR primers for primer information.


References

References

user report Kinoshita Y., Antitumor Effect on Murine Renal Cell Carcinoma by Autologous Tumor Vaccines Genetically Modified with Granulocyte-Macrophage Colony-Stimulating Factor and Interleukin-6 Cells. J. Immunother (1991)., 24, 205-211 (2001). PMID 11395635.
user report Nishisaka N., Immunotherapy for lung metastases of murine renal cell carcinoma: synergy between radiation and cytokine-producing tumor vaccines. J. Immunother., 22, 308-314 (1999). PMID 10404432.
user report Sakai T., DNA immunization with Plasmodium falciparum serine repeat antigen: regulation of humoral immune response by coinoculation of cytokine expression plasmid. Parasitol. Int., 48, 27-33 (1999). PMID 11269323.

2018.12.11

GNP_filter3_RDBDEP_html_181012.pl