RDB01413 - pAxCAmIL12p35

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pAxCAmIL12p35 (#RDB01413)

Shuttle vector to generate rAd expressing mouse IL-12 p35 cDNA

Alternative name	pAdex1CAmIL12p35
Clone info.	Shuttle vector to generate recombinant adenovirus vector expressing mouse IL-12p35 gene.
Comment	Produce adenovirus by COS-TPC method.
Vector backbone	pAxCAwt (Cosmid, use packaging extracts for transforming E. coli host)
Size of vector backbone	44,784 bp
Selectable markers	Amp^r
Gene/insert name	Mouse IL-12 p35 cDNA
Depositor\|Developer	Hamada, Hirofumi \|

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Distribution information

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Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Please visit Information of Request for Distribution.[link]

Terms and conditions for distribution The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Additional terms and conditions:
Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.

Remarks Remember that you will be working with samples containing infectious virus.

提供案内 (日本国内) [open/close]

Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	The availability of the BIOLOGICAL RESOURCE is limited to a RECIPIENT of a not-for profit institution for a not-for-profit research. RECIPIENT must contact the DEPOSITOR in the case of application for any patents or commercial use based on the results from the use of the BIOLOGICAL RESOURCE. In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of the following literature(s) designated by the DEPOSITOR is requested. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested. Additional terms and conditions: Regarding resources containing CAG promoter: In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation for the CAG promoter (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991) and an acknowledgment to Dr. Jun-ich Miyazaki of the Osaka University are requested.
Remarks	Remember that you will be working with samples containing infectious virus.

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
MTAに書く使用条件	非営利機関の非営利学術研究へ提供する。利用者が本件リソースを利用した研究成果に基づき特許申請、商業活動等を行う場合は、利用者は事前に寄託者に連絡する。利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。利用者は、研究成果の公表にあたって謝辞の表明を必要とする。 MTAに書く付加的使用条件： CAGプロモータを含むリソースについて: 利用者は、研究成果の公表にあたってCAGプロモータの文献 (Niwa, H., Yamamura, K., Miyazaki, J., Gene 108 : 193-200, 1991)を引用し、大阪大学宮崎純一博士への謝辞の表明を必要とする。
備考	このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01413	pAxCAmIL12p35	DNA solution	JPY 9,460 (not-for-profit academic purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pAxCAmIL12p35 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01413).

Reference section:

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB01413_B2L1p1-1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pAxCA_F1 (Pr0164) Region: insert 5' Sequence file: RDB01413_B2L1a.seq
>01413_01413_B2L1_3_pAxCA_F1_A01_01_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNTG CCTTCTTCTT TTTCCTACNG CTCCTGGGCA ACGTGCTGGT 61 TATTGTGCTG TCTCATCATT TTGGCAAAGC TCACCCTTAC CGAGTCGGCG ACACAGTGTG 121 GGTCCGCCGA CACCAGACTA AGAACCTAGA ACCTCGCTGG AAAGGACCTT ACACAGTCCT 181 GCTGACCACC CCCACCGCCC TCAAAGTAGA CGGCATCGCA GCTTGGATAC ACGCCGCCCA 241 CGTGAAGGCT GCCGACCCCG GGGGTGGACC ATCCTCTAGA CTGCCATGGG ATGTCAATCA 301 CGCTACCTCC TCTTTTTGGC CACCCTTGCC CTCCTAAACC ACCTCAGTTT GGCCAGGGTC 361 ATTCCAGTCT CTGGACCTGC CAGGTGTCTT AGCCAGTCCC GAAACCTGCT GAAGACCACA 421 GATGACATGG TGAAGACGGC CAGAGAAAAA CTGAAACATT ATTCCTGCAC TGCTGAAGAC 481 ATCGATCATG AAGACATCAC ACGGGACCAA ACCAGCACAT TGAAGACCTG TTTACCACTG 541 GAACTACACA AGAACGAGAG TTGCCTGGCT ACTAGAGAGA CTTCTTCCAC AACAAGAGGG 601 AGCTGCCTGC CCCCACAGAA GACGTCTTTG ATGATGACCC TGTGCCTTGG TAGCATCTAT 661 GAGGACTTGA AGANGTACCA GACAGAGTTC CAGGCCATCA ACGCAGCACT TCAGAATCAC 721 AACCATCAGC AGATCATTCT AGACAAGGGC ATGCTGGTGG CCATCGATGA NCTGATGCAG 781 TCTCTGAATC ATAATGGCGA GACTCTGCGC CAGAAACCTC CTGTGGGANA AGCANANCCT 841 TACAGANTGA AAATGAAGCT CTGCATCCTG CTTCACGCCT NCANCACCCG CNNCNNGNNC 901 NTCNNCAGGG NGATGGNNTA TCTGANCTCC GCNNNNNNAT CNGGANTANN CCNANTTNGN 961 NNAANANNNN NTCNNNTCAC NNCNNNNNNN NNN //
Primer: pAxCA_R1 (Pr0165) Region: insert 3' Sequence file: RDB01413_B2L1b.seq
>01413_01413_B2L1_3_pAxCA_R1_B01_04_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NCNAGCNNCN NCCTTCTGNT AGGCAGCCTG CACCTGANGN 61 GTGAATTGAT CCTGTCTTTA ACAAATTGGA CTAATCCGGA TCCTCAGGCG GAGCTCAGAT 121 AGCCCATCAC CCTGTTGATG GTCACGACGC GGGTGCTGAA GGCGTGAAGC AGGATGCAGA 181 GCTTCATTTT CACTCTGTAA GGGTCTGCTT CTCCCACAGG AGGTTTCTGG CGCAGAGTCT 241 CGCCATTATG ATTCAGAGAC TGCATCAGCT CATCGATGGC CACCAGCATG CCCTTGTCTA 301 GAATGATCTG CTGATGGTTG TGATTCTGAA GTGCTGCGTT GATGGCCTGG AACTCTGTCT 361 GGTACATCTT CAAGTCCTCA TAGATGCTAC CAAGGCACAG GGTCATCATC AAAGACGTCT 421 TCTGTGGGGG CAGGCAGCTC CCTCTTGTTG TGGAAGAAGT CTCTCTAGTA GCCAGGCAAC 481 TCTCGTTCTT GTGTAGTTCC AGTGGTAAAC AGGTCTTCAA TGTGCTGGTT TGGTCCCGTG 541 TGATGTCTTC ATGATCGATG TCTTCAGCAG TGCAGGAATA ATGTTTCAGT TTTTCTCTGG 601 CCGTCTTCAC CATGTCATCT GTGGTCTTCA GCAGGTTTCG GGACTGGCTA AGACACCTGG 661 CAGGTCCAGA GACTGGAATG ACCCTGGCCA AACTGAGGTG GTTTAGGAGG GCAAGGGTGG 721 CCAAAAAGAG GANGTAGCGT GATTGACATC CCATGGCAGT CTAGAGGATG GTCCACCCCN 781 GGGGTCGGCA GCCTTCACGT GNNCGGCGTG TATCCNAGCT GCNATGCCGT CTACTTTGAN 841 GGCGGNGGGG GTGGNCAGCA NGANTGTGTA ANGNCCTTNN CAGCGAGGTT NTANGTTCTT 901 ANNCTGGNGT CGGNNGANCC NCACTGNGTC GNNNANTCNG NNNNGNNNNN CTTTGNNNAA 961 ANGANGANNN NNNNNNN //
Primer: Amp_R (Pr0008) Region: AmpR pro,ITR Sequence file: RDB01413_B2L1c.seq
>01413_01413_B2L1_3_Amp_R_C01_07_ABI24.ab1 1 NNNNNNNNNN ANNANTNANC NNTTNNNNGG GTTNNTNNCT CNTGAGCGGA NACATATTTG 61 AATGNATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA 181 GGCCCTTTCG TCTTCAAGAA TTCCGGATCC GGGCCNATAT GATAATGANG GGGTGGANTT 241 TGTGACGNGG CGCGGGGNGN GGGAACGGGG NGGNNGNNGN ANNANNGNGG NNNAANNNNG 301 ANGNTNCANN NNNNNNNN //