Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Lyn/pLY30 (#RDB01306)

Plasmid clone of human LYN cDNA.

Clone info. Plasmid clone of human LYN cDNA.
Comment See Fig.2B of Yamanashi, Y. Mol. Cell. Biol., 1, 237-243 (1987) about insert sequence.
Vector backbone pUC9 (Plasmid)
Size of vector backbone 2,665 bp
Selectable markers Amp^r
Gene/insert name Human lyn (tyrosine Kinase) cDNA
Depositor|Developer Yamamoto, Tadashi |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution This material is provided with written consent of Developer.
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 樹立者に許可をとること。
備考 【追加提供依頼書】承諾書(FormD)
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01306 Lyn/pLY30 DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Lyn/pLY30 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01306).

Reference section:

Yamanashi, Y., Fukushige, S., Semba, K., Sukegawa, J., Miyajima, N., Matsubara, K., Yamamoto, T., Toyoshima, K., The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Mol. Cell. Biol., 7, 237-243 (1987). PMID 3561390. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB14383_A6Jpp1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB14383_A6Jpa.seq check
>01306_14383_A6Jp_M13(-40)_C05_03_ABI08.ab1
    1 CAGGCTGCAT CCGGGGACCG TCGACCTGCA GGGGGGGGGG GGGGGGTCTC GGCCGAGCCC
   61 AGAGACAGCC AGTTCCTCTC CCGCCGCGCC GGGCCGCGCT GCCGCTCGCT CCCCGGCCGT
  121 GGCGCCTCCG GGCCAGACGC GCTGCAGCCT CCAGCCCGCG GCAAGCGGGG CGGCCGCGCC
  181 ACCCCCGGCC CCGCGCCAGC AGCCCCTCGC CGCGCGTCCA GCGTTCCCGG CCAGCAGCCT
  241 CCCCATACGC AGGTCCTGCT GGACCGCCCC GTCGCGCCCC CCACTCTGAA CTCAAGTCAC
  301 CGTGGAGCTC CGCCGCCCCG AAACTTTCAC CGCGAGCGGG AAATATGGGA TGTATAAAAT
  361 CAAAAGGGAA AGACAGCTTG AGTGACGATG GAGTAGATTT GAAGACTCAA CCAGTACGTA
  421 ATACTGAAAG AACTATTTAT GTGAGAGATC CAACGTCCAA TAAACAGCAA AGGCCAGTTC
  481 CAGAATCTCA GCTTTTACCT GGACAGAGGT TTCAAACTAA AGATCCAGAG GAACAAGGAG
  541 ACATTGTGGT AGCCTTGTAC CCCTATGATG GCATCCACCC GGACGACTTG TCTTTCAAGA
  601 AAGGAGAGAA GATGAAAGTC CTGGAGGAGC ATGGAGAATG GTGGAAAGCA AAGTCCCTTT
  661 TAACAAAAAA AGAAGGCTTC ATCCCCAGCA ACTATGTGGC CAAACTCAAC ACCTTAGAAA
  721 CAGAAGAGTG GTTTTTCAAG GATATAACCA GGAAGGACGC AGAAAGGCAG CTTTTGGCAC
  781 CAGGAAATAG CGCTGGAGCT TTCCTTATTA GAGAAAGTGA AACATTAAAA GGAAGCTTCT
  841 CTCTGTCTGT CAGAGACTTT GACCCTGTGC ATGGGTGATG TTATTAAGCA CTACAAAATT
  901 AGAAAGTCTG GGATAATGGG GGCTATTTAC ATCTCTCCAC GAATCACTTT TCCCTGTATC
  961 AGCGACATGA TTAACATTTA CCAAAAGCAG CAGATGCTGT GCAGGAGATG CAGAGGCCTG
 1021 TAATAGTTCC AAGCCACAGA AGTCATTGGA TTATGATGCC TGGCAGATAC CCAGGAAGTC
1081 GATCAAGTGG T
//
Primer: Reverse2
Sequence file: RDB14383_A6Jpb.seq check
>01306_14383_A6Jp_Reverse2_F01_06_ABI08.ab1
    1 CAGACAGCAT TACGCCAAGC TTGGCTGCAG GGGGGGGGGG GGTTTTTTTT TTTTTTTAAA
   61 CAAGTAAGCC AAAGCACAAA GAAATGTCAT GCTATGTATA TATCCGGTTA TTTTAGCTTT
  121 ATAAATGAGT TTAGTTCTGC AATTATTGGG GATTCATTGC AATGGTCTCT GAAACTTTGA
  181 GTCCTATGTC CATTTGTAAA CATAGAGCTA GTTGGGTGCA TTTTTGGAGA GAATAGATGT
  241 TTTCTTCTCA AACGGCTGCA CTCAGATGTT GTCAAGCAGA GCAAGATTAC CAGCTGCTGA
  301 GGACAAGTCC AAGTTGCAAC TGAGTGGAGA ATCCATCTAA GAGTACAGAA TACTTTCCCA
  361 TTTAGAGGGT GTTCTTCCTG AGTAATTTAG CCTCTATTTC AGGATCCAGG CACGGCAGAT
  421 GATCCCCGCA CATGAAATCA TAAGTGCAAC CAGTGATGGT TACTTTTCCT CTCTAAATGA
  481 GGCAGCCACC CCTGCCAAAT GGACGGGTCT CCCTGTGCTC TAAGGCTGCT GCTGGTATTG
  541 CCCTTCCGTG GCTGTGTAGA AATCATCCAG GACGCTCTGT AAGTAGTCAA ACGTTGGTCT
  601 CTCTTCTGCC TTTTCTTTCC AGCACATTTT CATAATGTCA TAGAGCTCAT CTGGGCAGTT
  661 CTCCACACGG GGCATCCTGT AGCCCTGGGA CAGGGCGGTC ATCACGTCGG CATTAGTTCT
  721 CCCTGGGTAG GGAATTTTCC CATAGGTGAC AATTTCGTAT AGGAGGATTC CAAAGGACCA
  781 CACATCAGAC TTAATAGTGA AACATCCAAA GTTGATTGCT TCTGGAGCCG TCCACTTAAT
  841 AGGGAACTTA GCACCTTCCC TTGCTGTGTA CTCATTATCT TCAATTACTC TAGCAAGGCC
  901 AAATCTGCAA TTTTGCACAT TAGTGACTCG GAGACCAGAA CATTAGCTGC TCGCAGTTCC
  961 CGGGTGAATG TAGTTCTTCC GCTCGATGTA TGCCATTTCC CTCTGCATCT GAGCCAGAAA
 1021 AAGTCAATTG AGCCTTGAGC CAGGCAACTT TGCAACTTTC ATCGCTCTTC CAGGAATCAG
1081 CAACTGCCTA GGAAGTACTC AGTTGATGAT GTAAAATTAG
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Yamanashi, Y., The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Mol. Cell. Biol., 7, 237-243 (1987). PMID 3561390. [link to RRC of NBRP]
user_report Kinoshita-Kikuta, E., Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Sci. Rep. 10 (1): 16273 (2020). PMID 33004926. [link to RRC of NBRP]