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Lyn/pLY30

Plasmid clone of human LYN cDNA.

Catalog number RDB01306
Resource name Lyn/pLY30
Clone info. Plasmid clone of human LYN cDNA.
Comment See Fig.2B of Yamanashi, Y. Mol. Cell. Biol., 1, 237-243 (1987) about insert sequence.
Vector backbone pUC9 (Plasmid)
Size of vector backbone 2,665 bp
Selectable markers Amp^r
Gene/insert name Human lyn (tyrosine Kinase) cDNA
Depositor|Developer Yamamoto, Tadashi |
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External Database
Human lyn (tyrosine Kinase)

          Reference sequence
            

          Distribution information

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          RDB01306 Lyn/pLY30 DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01306.gif

          Original reference

          original Yamanashi, Y., The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Mol. Cell. Biol., 7, 237-243 (1987). PMID 3561390.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB14383_A6Jpp1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: M13_-40
          Sequence file: RDB14383_A6Jpa.seq check
          >01306_14383_A6Jp_M13(-40)_C05_03_ABI08.ab1
              1 CAGGCTGCAT CCGGGGACCG TCGACCTGCA GGGGGGGGGG GGGGGGTCTC GGCCGAGCCC
             61 AGAGACAGCC AGTTCCTCTC CCGCCGCGCC GGGCCGCGCT GCCGCTCGCT CCCCGGCCGT
            121 GGCGCCTCCG GGCCAGACGC GCTGCAGCCT CCAGCCCGCG GCAAGCGGGG CGGCCGCGCC
            181 ACCCCCGGCC CCGCGCCAGC AGCCCCTCGC CGCGCGTCCA GCGTTCCCGG CCAGCAGCCT
            241 CCCCATACGC AGGTCCTGCT GGACCGCCCC GTCGCGCCCC CCACTCTGAA CTCAAGTCAC
            301 CGTGGAGCTC CGCCGCCCCG AAACTTTCAC CGCGAGCGGG AAATATGGGA TGTATAAAAT
            361 CAAAAGGGAA AGACAGCTTG AGTGACGATG GAGTAGATTT GAAGACTCAA CCAGTACGTA
            421 ATACTGAAAG AACTATTTAT GTGAGAGATC CAACGTCCAA TAAACAGCAA AGGCCAGTTC
            481 CAGAATCTCA GCTTTTACCT GGACAGAGGT TTCAAACTAA AGATCCAGAG GAACAAGGAG
            541 ACATTGTGGT AGCCTTGTAC CCCTATGATG GCATCCACCC GGACGACTTG TCTTTCAAGA
            601 AAGGAGAGAA GATGAAAGTC CTGGAGGAGC ATGGAGAATG GTGGAAAGCA AAGTCCCTTT
            661 TAACAAAAAA AGAAGGCTTC ATCCCCAGCA ACTATGTGGC CAAACTCAAC ACCTTAGAAA
            721 CAGAAGAGTG GTTTTTCAAG GATATAACCA GGAAGGACGC AGAAAGGCAG CTTTTGGCAC
            781 CAGGAAATAG CGCTGGAGCT TTCCTTATTA GAGAAAGTGA AACATTAAAA GGAAGCTTCT
            841 CTCTGTCTGT CAGAGACTTT GACCCTGTGC ATGGGTGATG TTATTAAGCA CTACAAAATT
            901 AGAAAGTCTG GGATAATGGG GGCTATTTAC ATCTCTCCAC GAATCACTTT TCCCTGTATC
            961 AGCGACATGA TTAACATTTA CCAAAAGCAG CAGATGCTGT GCAGGAGATG CAGAGGCCTG
           1021 TAATAGTTCC AAGCCACAGA AGTCATTGGA TTATGATGCC TGGCAGATAC CCAGGAAGTC
          1081 GATCAAGTGG T
          //
          Primer: Reverse2
          Sequence file: RDB14383_A6Jpb.seq check
          >01306_14383_A6Jp_Reverse2_F01_06_ABI08.ab1
              1 CAGACAGCAT TACGCCAAGC TTGGCTGCAG GGGGGGGGGG GGTTTTTTTT TTTTTTTAAA
             61 CAAGTAAGCC AAAGCACAAA GAAATGTCAT GCTATGTATA TATCCGGTTA TTTTAGCTTT
            121 ATAAATGAGT TTAGTTCTGC AATTATTGGG GATTCATTGC AATGGTCTCT GAAACTTTGA
            181 GTCCTATGTC CATTTGTAAA CATAGAGCTA GTTGGGTGCA TTTTTGGAGA GAATAGATGT
            241 TTTCTTCTCA AACGGCTGCA CTCAGATGTT GTCAAGCAGA GCAAGATTAC CAGCTGCTGA
            301 GGACAAGTCC AAGTTGCAAC TGAGTGGAGA ATCCATCTAA GAGTACAGAA TACTTTCCCA
            361 TTTAGAGGGT GTTCTTCCTG AGTAATTTAG CCTCTATTTC AGGATCCAGG CACGGCAGAT
            421 GATCCCCGCA CATGAAATCA TAAGTGCAAC CAGTGATGGT TACTTTTCCT CTCTAAATGA
            481 GGCAGCCACC CCTGCCAAAT GGACGGGTCT CCCTGTGCTC TAAGGCTGCT GCTGGTATTG
            541 CCCTTCCGTG GCTGTGTAGA AATCATCCAG GACGCTCTGT AAGTAGTCAA ACGTTGGTCT
            601 CTCTTCTGCC TTTTCTTTCC AGCACATTTT CATAATGTCA TAGAGCTCAT CTGGGCAGTT
            661 CTCCACACGG GGCATCCTGT AGCCCTGGGA CAGGGCGGTC ATCACGTCGG CATTAGTTCT
            721 CCCTGGGTAG GGAATTTTCC CATAGGTGAC AATTTCGTAT AGGAGGATTC CAAAGGACCA
            781 CACATCAGAC TTAATAGTGA AACATCCAAA GTTGATTGCT TCTGGAGCCG TCCACTTAAT
            841 AGGGAACTTA GCACCTTCCC TTGCTGTGTA CTCATTATCT TCAATTACTC TAGCAAGGCC
            901 AAATCTGCAA TTTTGCACAT TAGTGACTCG GAGACCAGAA CATTAGCTGC TCGCAGTTCC
            961 CGGGTGAATG TAGTTCTTCC GCTCGATGTA TGCCATTTCC CTCTGCATCT GAGCCAGAAA
           1021 AAGTCAATTG AGCCTTGAGC CAGGCAACTT TGCAACTTTC ATCGCTCTTC CAGGAATCAG
          1081 CAACTGCCTA GGAAGTACTC AGTTGATGAT GTAAAATTAG
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Yamanashi, Y., The yes-related cellular gene lyn encodes a possible tyrosine kinase similar to p56lck. Mol. Cell. Biol., 7, 237-243 (1987). PMID 3561390.
          user_report Kinoshita-Kikuta, E., Protein-N-myristoylation-dependent phosphorylation of serine 13 of tyrosine kinase Lyn by casein kinase 1γ at the Golgi during intracellular protein traffic. Sci. Rep. 10 (1): 16273 (2020). PMID 33004926.

          2022.05.18

          GNP_filter3_RDBDEP_html_220516.pl