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PD-1 (cDNA#7) (#RDB01290)

cDNA from mouse T cell hybridoma

Alternative name cDNA from mouse T cell hybridoma
Clone info. Mouse T cell hybridoma programmed cell death 1 (Pdcd1) cDNA
Vector backbone pBluescript SK (-) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse programmed cell death 1 (Pdcd1) cDNA
Reference of insert sequence X67914 (DDBJ accession number)
Depositor|Developer Honjo, Tasuku | Ishida, Yasumasa |
 
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Terms and conditions for distribution For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp).
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01290 PD-1 (cDNA#7) DNA solution JPY 9,460 (not-for-profit academic purpose)
JPY 18,920 (for-profit-research purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The PD-1 (cDNA#7) was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01290).

Reference section:

Ishida, Y., Agata, Y., Shibahara, K., Honjo, T., Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J., 11, 3887-3895 (1992). PMID 1396582. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB17950_B0Bep1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: reverse-2 (Pr0100)
Region: MCS,insert5'
Sequence file: RDB17950_B0Bea.seq check
>01290_17950_B0Be_3_reverse2_G08_20_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNA ACNANNNNCN GGAGCTCCNC CGCGGTGGCG NNCGCTCTAG
   61 AACTAGTGGA TCCCCCGGGC TGCAGGAATT CGCGGCCGCT GAGCAGCGGG GAGGAGGAAG
  121 AGGAGACTGC TACTGAAGGC GACACTGCCA GGGGCTCTGG GCATGTGGGT CCGGCAGGTA
  181 CCCTGGTCAT TCACTTGGGC TGTGCTGCAG TTGAGCTGGC AATCAGGGTG GCTTCTAGAG
  241 GTCCCCAATG GGCCCTGGAG GTCCCTCACC TTCTACCCAG CCTGGCTCAC AGTGTCAGAG
  301 GGAGCAAATG CCACCTTCAC CTGCAGCTTG TCCAACTGGT CGGAGGATCT TATGCTGAAC
  361 TGGAACCGCC TGAGTCCCAG CAACCAGACT GAAAAACAGG CCGCCTTCTG TAATGGTTTG
  421 AGCCAACCCG TCCAGGATGC CCGCTTCCAG ATCATACAGC TGCCCAACAG GCATGACTTC
  481 CACATGAACA TCCTTGACAC ACGGCGCAAT GACAGTGGCA TCTACCTCTG TGGGGCCATC
  541 TCCCTGCACC CCAAGGCAAA AATCGAGGAG AGCCCTGGAG CAGAGCTCGT GGTAACAGAG
  601 AGAATCCTGG AGACCTCAAC AAGATATCCC AGCCCCTCGC CCAAACCAGA AGGCCGGTTT
  661 CAAGGCATGG TCATTGGTAT CATGAGTGCC CTAGTGGGTA TCCCTGTATT GCTGCTGCTG
  721 GCCTGGGCCC TANCTGTCTT CTGCTCAACA AGTATGTCAG ANGCCAGAGG ANCTGGAAGC
  781 AAGGACGACA CTCTGAANGA NGANCCTTCA NCAGCACCTG TCCCTANTGT GGCCTATGAN
  841 GANCTGGNNT TCCNNGGNNG ANANAAGANA CCAGANCTCC CNANCGCCNG NGNNNNCACA
  901 NAATATGNNN CCNTNNNCTT CNCNGNANNN NNNNGNNNNN NNNGNNNNNG NNNNNNNNGG
961 CNNNNNNNNN N
//
Primer: pUC-M13_-100 (Pr0310)
Region: MCS,insert3'
Sequence file: RDB17950_B0Beb.seq check
>01290_17950_B0Be_3_pUC-M13_-100_H08_23_ABI24.ab1
    1 NNNNNNNNNN NNNGGTTTTC CCNGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGTAA
   61 TACGACTCAC TATAGGGCGA ATTGGGTACC GGGCCCCCCC TCGAGGTCGA CGGTATCGAT
  121 AAGCTTGATA TCGAATTCGC GGCCGCTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTNNAAN NNTTTNGNNN NNNTTNNNNT NNNNNGGGNT NAANCCAAAN GAAAGGGANT
241 TANNCCCNGN NNNTTTTGNN NNNNNNNN
//
Primer: RDB01290#1 (Pr0441)
Region: insert mid(CDS 3')
Sequence file: RDB17950_B0Bec.seq check
>01290_17950_B0Be_3_RDB01290#1_G09_21_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNNGGCCATT GGCTATAGTG TCTCAAGTCC
   61 CTANNAGTGC CCAACAGTAG GATTCAGGAG ACATAGGCTT AGGCCCCAGC TCTGCACCTT
  121 GTGACAATAG GAAACCGGGA AGCCTGCTGC TCCCAGCTTG TGGTAAACCT GAAGTGAGCT
  181 CTCACTCAAC AGTGCATGTG CTGGAATTGG TGCAGGCGCT GGGCTGTCAC TCCAGCTAGC
  241 TCAGACCCTG GGAGCCTTGG CTGAACTGTA GCCTGCATGC TCCTCTTGAC TGAGGGACGG
  301 ACCGGTGCTC TCTGTGGAGG GTCTGCAGCA TGCTAATGGC TGAAGAATCT GGTCAAAGAG
  361 GCCAAGAACA ATGTCCATCC TCATGTCTTG GAGGCCGAGG ACCCTGCAGG CCATCAGCTG
  421 AGCCCCTACG TCCCATGGCC GAGGCACCCA GCCCTTCAGT GAAGACAATG GTGGCATATT
  481 CTGTGTGCAC ACAGGCGGTA GGGAGCTCTG GTGTCTTCTC TCGTCCCTGG AAGTCCAGCT
  541 CCTCATAGGC CACACTAGGG ACAGGTGCTG CTGAAGGCTC CTCCTTCAGA GTGTCGTCCT
  601 TGCTTCCAGC TCCTCTGGCC TCTGACATAC TTGTTGAGCA GAAGACAGCT AGGGCCCAGG
  661 CCAGCAGCAG CAATACAGGG NTACCCACTA GGGCACTCAT GATACCAATG ACCATGCCTT
  721 GAAACCGGCC TTCTGGTTTG GGCGANGGGC TGGGATATCT TGTTGANGTC TCCAGGANTC
  781 TCTCTGTTAC CACGAGCTCT GCTCCAGGGC TCTCCTCGAT TTTTGCCTTN NNGTGCANGG
  841 ANNTGGCCCC ACAGANGTAN ATGCCACTGT CATTGCGCCN NGTGTCAAGG ANGNTCATGT
  901 GNAANTCATG NNNGTNGGGC ANCTGNATGA TCTNNAANCG GNCATCCNNN NNNGNNTNNN
  961 NTCNAACNNT NACANNNNNN NGNNNNGTTT TNNNNNNNNG NNTNNNNNGN NNNNNNNNGN
1021 NNNNNNNNN
//
Primer: PD1-M_R (Pr0826)
Region: insert mid
Sequence file: RDB17950_B0Bed.seq check
>01290_17950_B0Be_3_PD1-M_R_G02_07_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCTTNTANG GNNNTGAGAN ACTATAGCCN
   61 ATGGCCTCTG TGGGTTCTGT GCCTGGAAAT GGAGAGATCT GAGTACAGCC TGCTTTGAAT
  121 GGCCCTGTGA GGCAACCCCA AAGCAAGGGG GTCCAGGTAT ACTATGGGCC CAGCACCTAA
  181 AGCCACCCTT GGGAGATGAT ACTCAGGTGG GAAATTCGTA GACTGGGGGA CTGAACCAAT
  241 CCCAAGATCT GGAAAAGTTT TGATGAAGAC TTGAAAAGCT CCTAGCTTCG GGGGTCTGGG
  301 AAGCATGAGC ACTTACCAGG CAAAAGCTCC GTGAGCGTAT CTGCTGTCCT TCTGCATGCC
  361 CAGGTACCTC AGTTTTTTTC AACAGCAAGG AAACTAGGGC AATAAAGGGA ACCAGCAGAG
  421 CTAGAGCCAC CCACACATCC NGGGGGGCAC TTGACTCTCC CTACTCCTCC TAGGAACCAA
  481 AAGGACAAAG TCCATGTTGA CAGCAGGGAA GGAAAGGGGG ANATAACCTT GACGCNNNCA
  541 ACACTGGGGT GTTAGAATCT CCTCATTCAC TCTGTCNTGN ANTTGGGTTC TGGCTCTCCT
  601 TCACACCTAG GANTCTGAAA TGANCAAGCA CTTCANANAG TCANGGTANC NAGANTCTAG
  661 CTGNCTGGTG GGNACCCAAA ATGANCAGGG NNTAAGTCCC TTTCNTTTNN NTTNAGCNNG
721 NNANANTNAA NNNNNCNNNA AGCTTTTNNN NANNNNNANN NNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Ishida, Y., Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J., 11, 3887-3895 (1992). PMID 1396582. [link to RRC of NBRP]