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PD-1 (cDNA#7)

cDNA from mouse T cell hybridoma

Catalog number RDB01290
Resource name PD-1 (cDNA#7)
Alternative name cDNA from mouse T cell hybridoma
Clone info. Mouse T cell hybridoma programmed cell death 1 (Pdcd1) cDNA
Vector backbone pBluescript SK (-) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Mouse programmed cell death 1 (Pdcd1) cDNA
Reference of insert sequence X67914 (DDBJ accession number)
Depositor|Developer Honjo, Tasuku | Ishida, Yasumasa |
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External Database
Mouse programmed cell death 1 (Pdcd1)

          Reference sequence
            

          Distribution information

          Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
          Terms and conditions set forth by the DEPOSITOR For use of the BIOLOGICAL RESOURCE by a for-profit institution, the RECIPIENT must obtain a prior written consent from the Kyoto University Medical Science and Business Liaison Organization Liaison Office (contracts@kumbl.med.kyoto-u.ac.jp).
          When requested by the DEPOSITOR, RIKEN BRC informs the DEPOSITOR on the name of resource, RECIPIENT's name and his/her affiliation, purpose of the use, and number of resources.
          Ordering Please visit Information of Request for Distribution.[link] 
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          Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word]
          提供案内 (日本国内) [open/close]

          提供条件 営利機関の利用者は、事前に京都大学・「医学領域」産学連携推進機構・知財室 (contracts@kumbl.med.kyoto-u.ac.jp) に連絡し、承諾を得ること。
          理研BRCは、本件リソースのリソース名、提供先、利用目的、件数を京都大学・本庶佑教授に報告する。
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          提供依頼書 [Word]
          提供同意書 (MTA、非営利機関による非営利学術研究用)[Word]

          Catalog # Resource name Shipping form Fee (non-profit org.)
          RDB01290 PD-1 (cDNA#7) DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01290.gif

          Original reference

          original Ishida, Y., Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J., 11, 3887-3895 (1992). PMID 1396582.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB17950_B0Bep1-1.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: reverse-2
          Region: MCS,insert5'
          Sequence file: RDB17950_B0Bea.seq check
          >01290_17950_B0Be_3_reverse2_G08_20_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNA ACNANNNNCN GGAGCTCCNC CGCGGTGGCG NNCGCTCTAG
             61 AACTAGTGGA TCCCCCGGGC TGCAGGAATT CGCGGCCGCT GAGCAGCGGG GAGGAGGAAG
            121 AGGAGACTGC TACTGAAGGC GACACTGCCA GGGGCTCTGG GCATGTGGGT CCGGCAGGTA
            181 CCCTGGTCAT TCACTTGGGC TGTGCTGCAG TTGAGCTGGC AATCAGGGTG GCTTCTAGAG
            241 GTCCCCAATG GGCCCTGGAG GTCCCTCACC TTCTACCCAG CCTGGCTCAC AGTGTCAGAG
            301 GGAGCAAATG CCACCTTCAC CTGCAGCTTG TCCAACTGGT CGGAGGATCT TATGCTGAAC
            361 TGGAACCGCC TGAGTCCCAG CAACCAGACT GAAAAACAGG CCGCCTTCTG TAATGGTTTG
            421 AGCCAACCCG TCCAGGATGC CCGCTTCCAG ATCATACAGC TGCCCAACAG GCATGACTTC
            481 CACATGAACA TCCTTGACAC ACGGCGCAAT GACAGTGGCA TCTACCTCTG TGGGGCCATC
            541 TCCCTGCACC CCAAGGCAAA AATCGAGGAG AGCCCTGGAG CAGAGCTCGT GGTAACAGAG
            601 AGAATCCTGG AGACCTCAAC AAGATATCCC AGCCCCTCGC CCAAACCAGA AGGCCGGTTT
            661 CAAGGCATGG TCATTGGTAT CATGAGTGCC CTAGTGGGTA TCCCTGTATT GCTGCTGCTG
            721 GCCTGGGCCC TANCTGTCTT CTGCTCAACA AGTATGTCAG ANGCCAGAGG ANCTGGAAGC
            781 AAGGACGACA CTCTGAANGA NGANCCTTCA NCAGCACCTG TCCCTANTGT GGCCTATGAN
            841 GANCTGGNNT TCCNNGGNNG ANANAAGANA CCAGANCTCC CNANCGCCNG NGNNNNCACA
            901 NAATATGNNN CCNTNNNCTT CNCNGNANNN NNNNGNNNNN NNNGNNNNNG NNNNNNNNGG
          961 CNNNNNNNNN N
          //
          Primer: pUC-M13_-100
          Region: MCS,insert3'
          Sequence file: RDB17950_B0Beb.seq check
          >01290_17950_B0Be_3_pUC-M13_-100_H08_23_ABI24.ab1
              1 NNNNNNNNNN NNNGGTTTTC CCNGTCACGA CGTTGTAAAA CGACGGCCAG TGAATTGTAA
             61 TACGACTCAC TATAGGGCGA ATTGGGTACC GGGCCCCCCC TCGAGGTCGA CGGTATCGAT
            121 AAGCTTGATA TCGAATTCGC GGCCGCTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
            181 TTTTTNNAAN NNTTTNGNNN NNNTTNNNNT NNNNNGGGNT NAANCCAAAN GAAAGGGANT
          241 TANNCCCNGN NNNTTTTGNN NNNNNNNN
          //
          Primer: RDB01290#1
          Region: insert mid(CDS 3')
          Sequence file: RDB17950_B0Bec.seq check
          >01290_17950_B0Be_3_RDB01290#1_G09_21_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNCNNNN NNNGGCCATT GGCTATAGTG TCTCAAGTCC
             61 CTANNAGTGC CCAACAGTAG GATTCAGGAG ACATAGGCTT AGGCCCCAGC TCTGCACCTT
            121 GTGACAATAG GAAACCGGGA AGCCTGCTGC TCCCAGCTTG TGGTAAACCT GAAGTGAGCT
            181 CTCACTCAAC AGTGCATGTG CTGGAATTGG TGCAGGCGCT GGGCTGTCAC TCCAGCTAGC
            241 TCAGACCCTG GGAGCCTTGG CTGAACTGTA GCCTGCATGC TCCTCTTGAC TGAGGGACGG
            301 ACCGGTGCTC TCTGTGGAGG GTCTGCAGCA TGCTAATGGC TGAAGAATCT GGTCAAAGAG
            361 GCCAAGAACA ATGTCCATCC TCATGTCTTG GAGGCCGAGG ACCCTGCAGG CCATCAGCTG
            421 AGCCCCTACG TCCCATGGCC GAGGCACCCA GCCCTTCAGT GAAGACAATG GTGGCATATT
            481 CTGTGTGCAC ACAGGCGGTA GGGAGCTCTG GTGTCTTCTC TCGTCCCTGG AAGTCCAGCT
            541 CCTCATAGGC CACACTAGGG ACAGGTGCTG CTGAAGGCTC CTCCTTCAGA GTGTCGTCCT
            601 TGCTTCCAGC TCCTCTGGCC TCTGACATAC TTGTTGAGCA GAAGACAGCT AGGGCCCAGG
            661 CCAGCAGCAG CAATACAGGG NTACCCACTA GGGCACTCAT GATACCAATG ACCATGCCTT
            721 GAAACCGGCC TTCTGGTTTG GGCGANGGGC TGGGATATCT TGTTGANGTC TCCAGGANTC
            781 TCTCTGTTAC CACGAGCTCT GCTCCAGGGC TCTCCTCGAT TTTTGCCTTN NNGTGCANGG
            841 ANNTGGCCCC ACAGANGTAN ATGCCACTGT CATTGCGCCN NGTGTCAAGG ANGNTCATGT
            901 GNAANTCATG NNNGTNGGGC ANCTGNATGA TCTNNAANCG GNCATCCNNN NNNGNNTNNN
            961 NTCNAACNNT NACANNNNNN NGNNNNGTTT TNNNNNNNNG NNTNNNNNGN NNNNNNNNGN
          1021 NNNNNNNNN
          //
          Primer: PD1-M_R
          Region: insert mid
          Sequence file: RDB17950_B0Bed.seq check
          >01290_17950_B0Be_3_PD1-M_R_G02_07_ABI08.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNNNN NNCTTNTANG GNNNTGAGAN ACTATAGCCN
             61 ATGGCCTCTG TGGGTTCTGT GCCTGGAAAT GGAGAGATCT GAGTACAGCC TGCTTTGAAT
            121 GGCCCTGTGA GGCAACCCCA AAGCAAGGGG GTCCAGGTAT ACTATGGGCC CAGCACCTAA
            181 AGCCACCCTT GGGAGATGAT ACTCAGGTGG GAAATTCGTA GACTGGGGGA CTGAACCAAT
            241 CCCAAGATCT GGAAAAGTTT TGATGAAGAC TTGAAAAGCT CCTAGCTTCG GGGGTCTGGG
            301 AAGCATGAGC ACTTACCAGG CAAAAGCTCC GTGAGCGTAT CTGCTGTCCT TCTGCATGCC
            361 CAGGTACCTC AGTTTTTTTC AACAGCAAGG AAACTAGGGC AATAAAGGGA ACCAGCAGAG
            421 CTAGAGCCAC CCACACATCC NGGGGGGCAC TTGACTCTCC CTACTCCTCC TAGGAACCAA
            481 AAGGACAAAG TCCATGTTGA CAGCAGGGAA GGAAAGGGGG ANATAACCTT GACGCNNNCA
            541 ACACTGGGGT GTTAGAATCT CCTCATTCAC TCTGTCNTGN ANTTGGGTTC TGGCTCTCCT
            601 TCACACCTAG GANTCTGAAA TGANCAAGCA CTTCANANAG TCANGGTANC NAGANTCTAG
            661 CTGNCTGGTG GGNACCCAAA ATGANCAGGG NNTAAGTCCC TTTCNTTTNN NTTNAGCNNG
          721 NNANANTNAA NNNNNCNNNA AGCTTTTNNN NANNNNNANN NNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Ishida, Y., Induced expression of PD-1, a novel member of the immunoglobulin gene superfamily, upon programmed cell death. EMBO J., 11, 3887-3895 (1992). PMID 1396582.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl