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pSV2 erb B2

Human erb B2 cDNA expression vector

Catalog number RDB01273
Resource name pSV2 erb B2
Alternative name Human erb B2 cDNA expression vector
Clone info. Human erb B2 cDNA driven by SV40 promoter
Vector backbone pSV2 (Plasmid)
Selectable markers Amp^r
Gene/insert name Human c-erbB2/erb-B cDNA
Depositor|Developer Yamamoto, Tadashi |
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External Database
Human c-erbB2/erb-B

          Reference sequence
            

          Distribution information

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          RDB01273 pSV2 erb B2 DNA solution

          Ordering Information [in Japanese] [in English]
          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          References and tips

          Electronic file

          Remarks, protocol and/or map (gif) RDB01273.gif

          Original reference

          original Masuko, T., A murine monoclonal antibody that recognizes an extracellular domain of the human c-erbB-2 protooncogene product. Jpn. J. Cancer Res. 80 (1): 10-14 (1989).original PMID 2565325.

          Further references such as user reports and related articles (go to bottom)

          Featured content


          Sequence information

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB13019_A4Khp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: SV40pro_F
          Region: SV40pro_ori,insert5'
          Sequence file: RDB13019_A4Kha.seq check
          >01273_13019_A4Kh_SV40pro_F_A05_02.ab1
              1 GGCTTGGCCT TTACATTCAG AGTAGTGAGG AGGCTTTTTT GGAGGCCTAG GCTTTTGCAA
             61 AAAGCTTGGG GCAGCCGCGC GCCCCTTCCC ACGGGGCCCT TTACTGCGCC GCGCGCCCGG
            121 CCCCCACCCC TCGCAGCACC CCGCGCCCCG CGCCCTCCCA GCCGGGTCCA GCCGGAGCCA
            181 TGGGGCCGGA GCCGCAGTGA GCACCATGGA GCTGGCGGCC TTGTGCCGCT GGGGGCTCCT
            241 CCTCGCCCTC TTGCCCCCCG GAGCCGCGAG CACCCAAGTG TGCACCGGCA CAGACATGAA
            301 GCTGCGGCTC CCTGCCAGTC CCGAGACCCA CCTGGACATG CTCCGCCACC TCTACCAGGG
            361 CTGCCAGGTG GTGCAGGGAA ACCTGGAACT CACCTACCTG CCCACCAATG CCAGCCTGTC
            421 CTTCCTGCAG GATATCCAGG AGGTGCAGGG CTACGTGCTC ATCGCTCACA ACCAAGTGAG
            481 GCAGGTCCCA CTGCAGAGGC TGCGGATTGT GCGAGGCACC CAGCTCTTTG AGGACAACTA
            541 TGCCCTGGCC GTGCTAGACA ATGGAGACCC GCTGAACAAT ACCACCCCTG TCACAGGGGC
            601 CTCCCCAGGA GGCCTGCGGG AGCTGCAGCT TCGAAGCCTC ACAGAGATCT TGAAAGGAGG
            661 GGTCTTGATC CAGCGGAACC CCCAGCTCTG CTACCAGGAC ACGATTTTGT GGAAGGACAT
            721 CTTCCACAAG AACAACCAGC TGGCTCTCAC ACTGATAGAC ACCAACCGCT CTCGGGCCTG
            781 CCACCCCTGT TCTCCGATGT GTAAGGGCTC CCGGTGCTGG GGAGAGAGTT CTGAGGATTG
            841 TCAGAGCCTG ACGCGCACTG TCTGTGCCGG TGGCTGTGCC CGCTGCAAGG GGCCACTGCC
            901 CACTGACTGC TGCCATGAGC AGTGTGCTGC CGGCTGCACG GGCCCCAAGC ACTCTGACTG
            961 CCTGGCCTGC CTCCACTTCA ACCACAGTGG CATCTGTGAG CTGCACTGCC CAGCCCTGGG
           1021 TCACCTACAA CACAGACACG TTTTGAGTCC ATGCCCAAAT CCCGAGGGCC CGGGTAATAC
           1081 ATTTCGGCGC CAGCTGTGTG AACTGCCCTG GTCCTTACAC TAACCTTTCC TACCGGAACG
          1141 TGGGAATCCT GCACCCCTTC CGGTTCCTTG CCCCCCCCCT TGTC
          //
          Primer: SV40-3'UTR
          Region: insert3'
          Sequence file: RDB13019_A4Khb.seq check
          >01273_13019_A4Kh_SV40-3'UTR_A05_02.ab1
              1 TCAACAGCAA GGTAGGTTCC TTCACAAAGA TCCAAGCTTG CAAGCTTGAA AAAAACAAAA
             61 CAAAAAAAGT AAAAACTAAA CAGAAAAGCA CTCTGTACAA AGCCTGGATA CTGACACCAT
            121 TGCTGTTCCT TCCTCATGGG GGGCAGTACT AGGTTTCAGG GACAGTCTCT GAATGGGTCG
            181 CTTTTGTTCT TAGACACTCC CTTAGGGCCG CTTCCCCTCT CAGGCCAGCT TCCCTAAGGC
            241 TTTCAGTACC CAGGATCTGG AAGGAAAGGG CCAAGCTGGA CTGTGGCATC CACTGGACCC
            301 TAGAGTCTCA TTGGGCAGGG CCTCAGAATC CACAAAGACT CCCCAGTGCT GTTCCTCTTC
            361 CAACGAGGCT GGACCCCTTC CAGCCATCTG GGAACTCAAG CAGGAAGGAA GGTTCCTTAG
            421 GACAGGTTCC TGGCATGGCA GGTTCCCCTG GAAGTGGTCG GAGGGCCCTC CCACCTCTTG
            481 ATGCCAGCAG AAGTCAGGCC TTCCCTGCTC CCTGAGGACA CATCAGGGCT TCTGCGGACT
            541 TGGCCTTCTG GTTCACACTG GCACGTCCAG ACCCAGGTAC TCTGGGTTCT CTGCCGTAGG
            601 TGTCCCTTTG AAGGTGCTGG GTGGAGCCCC CCGCTCTGGT GGGTCCTGGT CCCAGTAATA
            661 GAGGTTGTCG AAGGCTGGGC TGAAGGCAGG AGGAGGGTGG GGCTGAGGGG CAGCTCCTCC
            721 CTGGGGTGTC AAGTACTCGG GGTTCTCCAC GGCACCCCCA AAGGCAAAAA CGTCTTTGAC
            781 GACCCCATTC TTCCCTGGGG AGAGAGTCTT GGGCCTTTCC AGAGTGGCAC CAGCAGGTCG
            841 GGCAGCAGGC AGAGGGCCCT CTCGGGGCGA AGGGGGCTGG GGCCGAACAT CTGGCTGGTT
            901 CACATATTCA GGCTGGGGGC TGCAGGTCAG GGGGGCAACG TAGCCATCAG TCTCAGAGGG
            961 CAGGGGTACT GTGGGTCCTC ACTGTACCGC TGTAGAGGCT GGGGTCATGT GTGGGGAGGC
           1021 TTTGCAGCCC CTTGGCTGCC CCATTCCCAG GTCACCATCA ATACATCGGA GCCAGCCCCT
           1081 TTCGGAGGTG CCAGTGAGAC CTGGGGCCTC CTCACAGAGG GCTCAGCCTA GTGTCAGGTT
          1141 CCCCACGACC ACTCCTTGGT TAGAAATGAA GCCTTGTCCG
          //
          Primer: pAxCALNLF1
          Region: SV40p(A)
          Sequence file: RDB13019_A4Khc.seq check
          >01273_13019_A4Kh_pAxCALNL_F1_A12_03.ab1
              1 ACAATTTTTT CCTTAATTCA TGTTCTGGGA TCCCCAGGAA GCTCCTCTGT GTCCTCATAA
             61 ACCCTAACCT CCTCTACTTG AGAGGACATT CCAATCATAG GCTGCCCATC CACCCTCTGT
            121 GTCCTCCTGT TAATTAGGTC ACTTAACAAA AAGGAAATTG GGTAGGGGTT TTTCACAGAC
            181 CGCTTTCTAA GGGTAATTTT AAAATATCTG GGAAGTCCCT TCCACTGCTG TGTTCCAGAA
            241 GTGTTGGTAA ACAGCCCACA AATGTCAACA GCAGAAACAT ACAAGCTGTC AGCTTTGCAC
            301 AAGGGCCCAA CACCCTGCTC ATCAAGAAGC ACTGTGGTTG CTGTGTTAGT AATGTGCAAA
            361 ACAGGAGGCA CATTTTCCCC ACCTGTGTAG GTTCCAAAAT ATCTAGTGTT TTCATTTTTA
            421 CTTGGATCAG GAACCCAGCA CTCCACTGGA TAAGCATTAT CCTTATCCAA AACAGCCTTG
            481 TGGTCAGTGT TCATCTGCTG ACTGTCAACT GTAGCATTTT TTGGGGTTAC AGTTTGAGCA
            541 GGATATTTGG TCCTGTAGTT TGCTAACACA CCCTGCAGCT CCAAAGGTTC CCCACCAACA
            601 GCAAAAAAAT GAAAATTTGA CCCTTGAATG GGTTTTCCAG CACCATTTTC ATGAGTTTTT
            661 TGTGTCCCTG AATGCAAGTT TAACATAGCA GTTACCCCAA TAACCTCAGT TTTAACAGTA
            721 AACAGCTTCC CACATCAAAA TATTTCCACA GGTTAAAGTC CTCATTTAAA TTAGGCAAAG
            781 GAATTCTTGA AAGACGAAAG GGCCCTCGTG ATACGCCTAT TTTTATAGGT TTATGTCATG
            841 ATAATAATGG TTTCTTAAAC GTCAGGTGGC ACCTTTTCGG GGGAAAATGT GCGCGGAACC
          901 CCTATCTTGT ATATTTTTTC TAAAAT
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Masuko, T., A murine monoclonal antibody that recognizes an extracellular domain of the human c-erbB-2 protooncogene product. Jpn. J. Cancer Res. 80 (1): 10-14 (1989).original PMID 2565325.
          reference Matsuda, S., 17 beta-estradiol mimics ligand activity of the c-erbB2 protooncogene product. Proc. Natl. Acad. Sci. U.S.A. 90 (22): 10803-10807 (1993). PMID 7902571.
          reference Ullrich, A., Human epidermal growth factor receptor cDNA sequence and aberrant expression of the amplified gene in A431 epidermoid carcinoma cells. Nature 319, 230-234 (1986). PMID 6328312.
          user report Miura, T., Ligand-inducible dimeric antibody for selecting antibodies against a membrane protein based on mammalian cell proliferation. Biotechnol. Bioeng. 113 (5): 1113-1123 (2016). PMID 26479395.

          2022.02.09

          GNP_filter3_RDBDEP_html_220112.pl