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pHTH1 (#RDB01269)

Human tyrosine hydroxylase type 1 cDNA

Alternative name Human tyrosine hydroxylase type I cDNA
Clone info. Human tyrosine hydroxylase type I cDNA from human pheochromocytoma cDNA library
Vector backbone pBluescript KS (-) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Human TH/Tyrosine hydroxylase cDNA
Depositor|Developer Nagatsu, Toshiharu |
 
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01269 pHTH1 DNA solution JPY 9,460 (not-for-profit academic purpose)
plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHTH1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01269).

Reference section:

Kaneda, N., Kobayashi, K., Ichinose, H., Kishi, F., Nakazawa, A., Kurosawa, Y., Fujita, K., Nagatsu, T., Isolation of a novel cDNA clone for human tyrosine hydroxylase: alternative RNA splicing produces four kinds of mRNA from a single gene. Biochem. Biophys. Res. Commun., 146, 971-975 (1987). PMID 2887169.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB15497_A7K9p1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2
Sequence file: RDB15497_A7K9a.seq check
>01269_15479_A7K9_Reverse2_A02_01_ABI08.ab1
    1 AGTTAAAATG AATTACGGCC AAGCTCGAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT
   61 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CCCACTGAGC
  121 CATGCCCACC CCCGACGCCA CCACGCCACA GGCCAAGGGC TTCCGCAGGG CCGTGTCTGA
  181 GCTGGACGCC AAGCAGGCAG AGGCCATCAT GTCCCCGCGG TTCATTGGGC GCAGGCAGAG
  241 CCTCATCGAG GACGCCCGCA AGGAGCGGGA GGCGGCGGTG GCAGCAGCGG CCGCTGCAGT
  301 CCCCTCGGAG CCCGGGGACC CCCTGGAGGC TGTGGCCTTT GAGGAGAAGG AGGGGAAGGC
  361 CGTGCTAAAC CTGCTCTTCT CCCCGAGGGC CACCAAGCCC TCGGCGCTGT CCCGAGCTGT
  421 GAAGGTGTTT GAGACGTTTG AAGCCAAAAT CCACCATCTA GAGACCCGGC CCGCCCAGAG
  481 GCCGCGAGCT GGGGGCCCCC ACCTGGAGTA CTTCGTGCGC CTCGAGGTGC GCCGAGGGGA
  541 CCTGGCCGCC CTGCTCAGTG GTGTGCGCCA GGTGTCAGAG GACGTGCGCA GCCCCGCGGG
  601 GCCCAAGGTC CCCTGGTTCC CAAGAAAAGT GTCAGAGCTG GACAAGTGTC ATCACCTGGT
  661 CACCAAGTTC GACCCTGACC TGGACTTGGA CCACCCGGGC TTCTCGGACC AGGTGTACCG
  721 CCAGCGCAGG AAGCTGATTG CTGAGATCGC CTTCCAGTAC AGGCACGGCG ACCCGATTCC
  781 CCGTGTGGAG TACACCGCCG AGGAGATTGC CACCTGGAAG GAGGTCTACA CCACGCTGAA
  841 GGGCCTCTAC GCCACGCACG CCTGCGGGGA GCACCTGGAG GCCTTTGCTT TGCTGGAGCG
  901 CTTCAGCGGC TACCGGGAAG ACAATATCCC CCAGCTGGAG GACGTCTCCC GCTTCCTGAA
  961 GGAGCGCACG GGCTTCCAGC TGCGGCCTGT GGCCGGGCCT GCTGTCCGCC CGGGACTTCC
 1021 CTGGCCAGCC CTGGCCTTCC GCGTGTTCCC AGTGCACCCA GTATATCCGC CACGCGTCCT
 1081 CGCCCATGCA CTCCCCTGAG CCCGGACTGC TGCCACGAGC CTGCCTGGGG GCCACGTGCC
1141 CAATGCTGGC CGGAACCCGC CACACCATTT CG
//
Primer: RDB01269#1
Sequence file: RDB15497_A7K9b.seq check
>01269_15497_2_A7K9_RDB01269#1_A01_01_ABI08.ab1
    1 CTACTAAGCA AGCCTGCGGG GGGAGCACCT GGAGGCCTTT GCTTTGCTGG AGCGCTTCAG
   61 CGGCTACCGG GAAGACAATA TCCCCCAGCT GGAGGACGTC TCCCGCTTCC TGAAGGAGCG
  121 CACGGGCTTC CAGCTGCGGC CTGTGGCCGG CCTGCTGTCC GCCCGGGACT TCCTGGCCAG
  181 CCTGGCCTTC CGCGTGTTCC AGTGCACCCA GTATATCCGC CACGCGTCCT CGCCCATGCA
  241 CTCCCCTGAG CCGGACTGCT GCCACGAGCT GCTGGGGCAC GTGCCCATGC TGGCCGACCG
  301 CACCTTCGCG CAGTTCTCGC AGGACATTGG CCTGGCGTCC CTGGGGGCCT CGGATGAGGA
  361 AATTGAGAAG CTGTCCACGC TGTACTGGTT CACGGTGGAG TTCGGGCTGT GTAAGCAGAA
  421 CGGGGAGGTG AAGGCCTATG GTGCCGGGCT GCTGTCCTCC TACGGGGAGC TCCTGCACTG
  481 CCTGTCTGAG GAGCCTGAGA TTCGGGCCTT CGACCCTGAG GCTGCGGCCG TGCAGCCCTA
  541 CCAAGACCAG ACGTACCAGT CAGTCTACTT CGTGTCTGAG AGCTTCAGTG ACGCCAAGGA
  601 CAAGCTCAGG AGCTATGCCT CACGCATCCA GCGCCCCTTC TCCGTGAAGT TCGACCCGTA
  661 CACGCTGGCC ATCGACGTGC TGGACAGCCC CCAGGCCGTG CGGCGCTCCC TGGAGGGTGT
  721 CCAGGATGAG CTGGACACCC TTGCCCATGC GCTGAGTGCC ATTGGCTAGG TGCACGGCGT
  781 CCCTGAGGGC CCTTCCCAAC CTCCCCTGGT CCTGCACTGT CCCGGAGCTC AGGCCCTGGT
  841 GAGGGGCTGG GTCCCGGGTG CCCCCCATGC CCTCCCTGCT GCCAGGCTCC CACTGCCCCT
  901 GCACCTGCTT CTCAGCGCAA CAGCTGTGTG TGCCCGTGGT GAGGTTGTGC TGCCTGTGGT
  961 GAGGTCCTGT CCTGGCTCCC AGGGTCCTGG GGGGCTGCTG CACTGCCCTC CCGCCCTTCC
 1021 CTGACACTGT CTGCTGCCCC CAATCACCCG TCACATAAAG AACTGTGGTC TCTGTAGAAG
 1081 AAAGGAGAAG AGAGGAACGA GTAGTAGTCG CATACGACCG AGTGAGAGCA AGTACTGATA
1141 TAATAGTAGT AGTAGAAGAT
//
Primer: RDB01269#2
Sequence file: RDB15497_A7K9c.seq check
>01269_15497_2_A7K9_RDB01269#2_B01_02_ABI08.ab1
    1 GGGGGGCGTT CTCGTGAGGT TCGACCCGTA CACGCTGGCC ATCGACGTGC TGGACAGCCC
   61 CCAGGCCGTG CGGCGCTCCC TGGAGGGTGT CCAGGATGAG CTGGACACCC TTGCCCATGC
  121 GCTGAGTGCC ATTGGCTAGG TGCACGGCGT CCCTGAGGGC CCTTCCCAAC CTCCCCTGGT
  181 CCTGCACTGT CCCGGAGCTC AGGCCCTGGT GAGGGGCTGG GTCCCGGGTG CCCCCCATGC
  241 CCTCCCTGCT GCCAGGCTCC CACTGCCCCT GCACCTGCTT CTCAGCGCAA CAGCTGTGTG
  301 TGCCCGTGGT GAGGTTGTGC TGCCTGTGGT GAGGTCCTGT CCTGGCTCCC AGGGTCCTGG
  361 GGGCTGCTGC ACTGCCCTCC GCCCTTCCCT GACACTGTCT GCTGCCCCAA TCACCGTCAC
  421 AATAAAAGAA ACTGTGGTCT CTAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA
  481 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAGGGA
  541 TTTCCTGCAC CCCGGGGGAT CCCCTATTTC TAAAGCGGCC GCCCCCGCGG GGGAGCTCCA
  601 ATTCCCCCTA TAGTGAGTCG TATTACAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT
  661 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCTT TTCGCCAGCT
  721 GGGGTAATAG CGAAAAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG
  781 GCGAATGGAA ATTGTAAGCG TTAATATTTT TGTTAAAATT TCGCGTTAAA TTTTTGTTAA
  841 ATCAGCCTCA TTTTTTTTAA CCAATAGGGC CGAAATCGGG CAAAAATCCC TTTATAATCA
  901 AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGACCAAAGA GTCCACTATT
  961 TAAAAGACGG TGGACTCCCA ACGTCCAAGG GCGAAAAACC CGTCTTATTC GAGGGCGATG
 1021 GTCCACTACG TGGAACCATC ACCCTAATCA AGTTTTTTTG GGGGGTCGAG GGTGCCGGTA
1081 AGGCACTAAT CGATCCTTAA GGGAAGACCC CGATTTTAGA AGCTTTGTG
//
Primer: M13_-40
Sequence file: RDB15497_A7K9d.seq check
>01269_15479_A7K9_M13_-40_B02_02_ABI08.ab1
    1 AAAGCAAGTG AATTGGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCC ACCGCGGTGG
   61 CGGCCGCTCT AGAACTAGTG GATCCCCCGG GCTGCAGGAA TTCCTTTTTT TTTTTTTTTT
  121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT
  181 TTTTTTTTTT TTTTTTTTTA AAAACCCCCT TTTTTTTTTT TGGGACGGGG ATTGGGGCCC
  241 CAAAAAGGGG CCGGGAAGGG GGGAGGGGAG GGCACCCCCC CCCCAAACCC TGGGAGCCCA
  301 AAAAGGACCC CCCCCCAGGG GGCAAAACCC CCCCCCGGGG ACACACAATT TTTTCCCTAA
  361 AAAACAGGGG GAGGGGCAGG GGGAACCTGG CAACAGGGAG GGCATGGGGG GCCCCCGGGA
  421 CCCCCCCCCC CCCCAGGGCC TGAACTCCGG GAAAGTGGAA AAACAGGGGA GGTTGGGAAG
  481 GGCCCCCAGG GACCCCCGGC CCCTACCCAA GGGGCCCCCC CCCAGGGGGA AGGGGGTCCC
  541 CCTCTTCCTG GAACCCCCCC AGGGAACCCC CCCCGGCCTG GGGGGTTTCC CACCCCTCAA
  601 TGGCCCCCGT GTACGGGTCA AAATTTCCGA AAAAGGGGCC CTGGATGCCT GAAGGATAAC
  661 TTCTGAACTT GGCCTTGGCG TCACTGAAAC TCTCAAACAC AAAATAAACT GACTGGTACC
  721 TCTGGCCCTG GGAGGGTGCC CCCCCCCCCC TCGGGTCAAA AGCCCAATTC CGGCTCCTCA
  781 AAAAGGGAGT GCGGAACTCC CCGAAAGAGA GACACAGCCC GCACCATAGC CTTCACTCCC
  841 CGTCTGTTTT ACACGCCCGA AATTCCCGGG ACCAGTACGC GTGAACGCTT CCTATATTCC
  901 CTATCACGAG GCCTCAGGGA CGCCAGCAAT GTCTTGCGAA ACTGCGCGAA GGTGCGGTCG
  961 CCACATGGAC ACGTGCTCAA CACTCTGGTC ACACTCGGGT AAGGATCATG GCGAGACGTG
 1021 CGTATATACT GTGCATGTAC CCAGAGCTCA AGCTGTCAGA GTCGCGACAG AAGTCTGAAT
1081 CCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Kaneda, N., Isolation of a novel cDNA clone for human tyrosine hydroxylase: alternative RNA splicing produces four kinds of mRNA from a single gene. Biochem. Biophys. Res. Commun., 146, 971-975 (1987). PMID 2887169.
user_report Takekoshi K., Stimulation of catecholamine biosynthesis via the protein kinase C pathway by endothelin-1 in PC12 rat pheochromocytoma cells. Biochem. Pharmacol., 63, 977-984 (2002). PMID 11911850. [link to RRC of NBRP]
user_report Takekoshi K., Ca(2+) mobilization, tyrosine hydroxylase activity, and signaling mechanisms in cultured porcine adrenal medullary chromaffin cells: effects of leptin. Endocrinology, 142, 290-298 (2001). PMID 11145592. [link to RRC of NBRP]
user_report Takekoshi K., Leptin stimulates catecholamine synthesis in a PKC-dependent manner in cultured porcine adrenal medullary chromaffin cells. Endocrinology, 142, 4861-4871 (2001). PMID 11606454. [link to RRC of NBRP]
user_report Takekoshi K., Effects of natriuretic peptides (ANP, BNP, CNP) on catecholamine synthesis and TH mRNA levels in PC12 cells. Life Sci., 66, PL303-311 (2000). PMID 10834306. [link to RRC of NBRP]
user_report Takekoshi K., kappa-Opioid inhibits catecholamine biosynthesis in PC12 rat pheochromocytoma cell. FEBS Lett., 477, 273-277 (2000). PMID 10908734. [link to RRC of NBRP]
user_report Isobe K., Expression of mRNA coding for four catecholamine-synthesizing enzymes in human adrenal pheochromocytomas. Eur. J. Endocrinol., 138, 383-387 (1998). PMID 9578504. [link to RRC of NBRP]
user_report Yamakuni T., A novel protein containing Cdc10/SWI6 motifs regulates expression of mRNA encoding catecholamine biosynthesizing enzymes. J. Biol. Chem., 273, 27051-27054 (1998). PMID 9765218. [link to RRC of NBRP]
user_report Isobe K., Pituitary adenylate cyclase-activating polypeptide induces gene expression of the catecholamine synthesizing enzymes, tyrosine hydroxylase and dopamine beta hydroxylase, through 3',5'-cyclic adenosine monophosphate- and protein kinase C-dependent mechanisms in cultured porcine adrenal medullary chromaffin cells. Neuropeptides., 30, 167-175 (1996). PMID 8771559. [link to RRC of NBRP]
user_report Isobe K., Expression of mRNAs coding for catecholamine synthesizing enzymes in human adrenal pheochromocytoma. Jpn. J. Clin. Pathol., 43, 540-544 (1995). PMID 7602797. [link to RRC of NBRP]