RDB01269 - pHTH1

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pHTH1

Human tyrosine hydroxylase type 1 cDNA

Catalog number	RDB01269
Resource name	pHTH1
Alternative name	Human tyrosine hydroxylase type I cDNA
Clone info.	Human tyrosine hydroxylase type I cDNA from human pheochromocytoma cDNA library
Vector backbone	pBluescript KS (-) (Plasmid)
Size of vector backbone	3.0 kb
Selectable markers	Amp^r
Gene/insert name	Human TH/Tyrosine hydroxylase cDNA
Depositor\|Developer	Nagatsu, Toshiharu \|

Other clones in our bank

human TH/Tyrosine hydroxylase

External Database
Human TH/Tyrosine hydroxylase

Reference sequence

Remarks, protocol and/or map (gif)	RDB01269.gif

Distribution information

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Terms and conditions for distribution	This material is provided with written consent of Depositor.
Ordering	Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. Order form [Credit Card Payment] [Bank Transfer Payment] Material Transfer Agreement (MTA for use for not-for-profit academic purpose) [Word] Approval form by depositor [Word]
Remarks	((Additional form)) Approval Form(FormD) Please obtain written approval of the depositor.

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備考	【追加提供依頼書】承諾書(FormD) 寄託者の書面による提供承諾が必要です。

Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01269	pHTH1	DNA solution

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pHTH1 was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01269).

Reference section:

Kaneda, N., Kobayashi, K., Ichinose, H., Kishi, F., Nakazawa, A., Kurosawa, Y., Fujita, K., Nagatsu, T., Isolation of a novel cDNA clone for human tyrosine hydroxylase: alternative RNA splicing produces four kinds of mRNA from a single gene. Biochem. Biophys. Res. Commun., 146, 971-975 (1987). PMID 2887169.

Further references such as user reports and related articles (go to bottom)

References and tips

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB15497_A7K9p1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Reverse2 Sequence file: RDB15497_A7K9a.seq
>01269_15479_A7K9_Reverse2_A02_01_ABI08.ab1 1 AGTTAAAATG AATTACGGCC AAGCTCGAAT TAACCCTCAC TAAAGGGAAC AAAAGCTGGT 61 ACCGGGCCCC CCCTCGAGGT CGACGGTATC GATAAGCTTG ATATCGAATT CCCACTGAGC 121 CATGCCCACC CCCGACGCCA CCACGCCACA GGCCAAGGGC TTCCGCAGGG CCGTGTCTGA 181 GCTGGACGCC AAGCAGGCAG AGGCCATCAT GTCCCCGCGG TTCATTGGGC GCAGGCAGAG 241 CCTCATCGAG GACGCCCGCA AGGAGCGGGA GGCGGCGGTG GCAGCAGCGG CCGCTGCAGT 301 CCCCTCGGAG CCCGGGGACC CCCTGGAGGC TGTGGCCTTT GAGGAGAAGG AGGGGAAGGC 361 CGTGCTAAAC CTGCTCTTCT CCCCGAGGGC CACCAAGCCC TCGGCGCTGT CCCGAGCTGT 421 GAAGGTGTTT GAGACGTTTG AAGCCAAAAT CCACCATCTA GAGACCCGGC CCGCCCAGAG 481 GCCGCGAGCT GGGGGCCCCC ACCTGGAGTA CTTCGTGCGC CTCGAGGTGC GCCGAGGGGA 541 CCTGGCCGCC CTGCTCAGTG GTGTGCGCCA GGTGTCAGAG GACGTGCGCA GCCCCGCGGG 601 GCCCAAGGTC CCCTGGTTCC CAAGAAAAGT GTCAGAGCTG GACAAGTGTC ATCACCTGGT 661 CACCAAGTTC GACCCTGACC TGGACTTGGA CCACCCGGGC TTCTCGGACC AGGTGTACCG 721 CCAGCGCAGG AAGCTGATTG CTGAGATCGC CTTCCAGTAC AGGCACGGCG ACCCGATTCC 781 CCGTGTGGAG TACACCGCCG AGGAGATTGC CACCTGGAAG GAGGTCTACA CCACGCTGAA 841 GGGCCTCTAC GCCACGCACG CCTGCGGGGA GCACCTGGAG GCCTTTGCTT TGCTGGAGCG 901 CTTCAGCGGC TACCGGGAAG ACAATATCCC CCAGCTGGAG GACGTCTCCC GCTTCCTGAA 961 GGAGCGCACG GGCTTCCAGC TGCGGCCTGT GGCCGGGCCT GCTGTCCGCC CGGGACTTCC 1021 CTGGCCAGCC CTGGCCTTCC GCGTGTTCCC AGTGCACCCA GTATATCCGC CACGCGTCCT 1081 CGCCCATGCA CTCCCCTGAG CCCGGACTGC TGCCACGAGC CTGCCTGGGG GCCACGTGCC 1141 CAATGCTGGC CGGAACCCGC CACACCATTT CG //
Primer: RDB01269#1 Sequence file: RDB15497_A7K9b.seq
>01269_15497_2_A7K9_RDB01269#1_A01_01_ABI08.ab1 1 CTACTAAGCA AGCCTGCGGG GGGAGCACCT GGAGGCCTTT GCTTTGCTGG AGCGCTTCAG 61 CGGCTACCGG GAAGACAATA TCCCCCAGCT GGAGGACGTC TCCCGCTTCC TGAAGGAGCG 121 CACGGGCTTC CAGCTGCGGC CTGTGGCCGG CCTGCTGTCC GCCCGGGACT TCCTGGCCAG 181 CCTGGCCTTC CGCGTGTTCC AGTGCACCCA GTATATCCGC CACGCGTCCT CGCCCATGCA 241 CTCCCCTGAG CCGGACTGCT GCCACGAGCT GCTGGGGCAC GTGCCCATGC TGGCCGACCG 301 CACCTTCGCG CAGTTCTCGC AGGACATTGG CCTGGCGTCC CTGGGGGCCT CGGATGAGGA 361 AATTGAGAAG CTGTCCACGC TGTACTGGTT CACGGTGGAG TTCGGGCTGT GTAAGCAGAA 421 CGGGGAGGTG AAGGCCTATG GTGCCGGGCT GCTGTCCTCC TACGGGGAGC TCCTGCACTG 481 CCTGTCTGAG GAGCCTGAGA TTCGGGCCTT CGACCCTGAG GCTGCGGCCG TGCAGCCCTA 541 CCAAGACCAG ACGTACCAGT CAGTCTACTT CGTGTCTGAG AGCTTCAGTG ACGCCAAGGA 601 CAAGCTCAGG AGCTATGCCT CACGCATCCA GCGCCCCTTC TCCGTGAAGT TCGACCCGTA 661 CACGCTGGCC ATCGACGTGC TGGACAGCCC CCAGGCCGTG CGGCGCTCCC TGGAGGGTGT 721 CCAGGATGAG CTGGACACCC TTGCCCATGC GCTGAGTGCC ATTGGCTAGG TGCACGGCGT 781 CCCTGAGGGC CCTTCCCAAC CTCCCCTGGT CCTGCACTGT CCCGGAGCTC AGGCCCTGGT 841 GAGGGGCTGG GTCCCGGGTG CCCCCCATGC CCTCCCTGCT GCCAGGCTCC CACTGCCCCT 901 GCACCTGCTT CTCAGCGCAA CAGCTGTGTG TGCCCGTGGT GAGGTTGTGC TGCCTGTGGT 961 GAGGTCCTGT CCTGGCTCCC AGGGTCCTGG GGGGCTGCTG CACTGCCCTC CCGCCCTTCC 1021 CTGACACTGT CTGCTGCCCC CAATCACCCG TCACATAAAG AACTGTGGTC TCTGTAGAAG 1081 AAAGGAGAAG AGAGGAACGA GTAGTAGTCG CATACGACCG AGTGAGAGCA AGTACTGATA 1141 TAATAGTAGT AGTAGAAGAT //
Primer: RDB01269#2 Sequence file: RDB15497_A7K9c.seq
>01269_15497_2_A7K9_RDB01269#2_B01_02_ABI08.ab1 1 GGGGGGCGTT CTCGTGAGGT TCGACCCGTA CACGCTGGCC ATCGACGTGC TGGACAGCCC 61 CCAGGCCGTG CGGCGCTCCC TGGAGGGTGT CCAGGATGAG CTGGACACCC TTGCCCATGC 121 GCTGAGTGCC ATTGGCTAGG TGCACGGCGT CCCTGAGGGC CCTTCCCAAC CTCCCCTGGT 181 CCTGCACTGT CCCGGAGCTC AGGCCCTGGT GAGGGGCTGG GTCCCGGGTG CCCCCCATGC 241 CCTCCCTGCT GCCAGGCTCC CACTGCCCCT GCACCTGCTT CTCAGCGCAA CAGCTGTGTG 301 TGCCCGTGGT GAGGTTGTGC TGCCTGTGGT GAGGTCCTGT CCTGGCTCCC AGGGTCCTGG 361 GGGCTGCTGC ACTGCCCTCC GCCCTTCCCT GACACTGTCT GCTGCCCCAA TCACCGTCAC 421 AATAAAAGAA ACTGTGGTCT CTAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA 481 AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAAAAA AAAAAAGGGA 541 TTTCCTGCAC CCCGGGGGAT CCCCTATTTC TAAAGCGGCC GCCCCCGCGG GGGAGCTCCA 601 ATTCCCCCTA TAGTGAGTCG TATTACAATT CACTGGCCGT CGTTTTACAA CGTCGTGACT 661 GGGAAAACCC TGGCGTTACC CAACTTAATC GCCTTGCAGC ACATCCCCTT TTCGCCAGCT 721 GGGGTAATAG CGAAAAGGCC CGCACCGATC GCCCTTCCCA ACAGTTGCGC AGCCTGAATG 781 GCGAATGGAA ATTGTAAGCG TTAATATTTT TGTTAAAATT TCGCGTTAAA TTTTTGTTAA 841 ATCAGCCTCA TTTTTTTTAA CCAATAGGGC CGAAATCGGG CAAAAATCCC TTTATAATCA 901 AAAGAATAGA CCGAGATAGG GTTGAGTGTT GTTCCAGTTT GGACCAAAGA GTCCACTATT 961 TAAAAGACGG TGGACTCCCA ACGTCCAAGG GCGAAAAACC CGTCTTATTC GAGGGCGATG 1021 GTCCACTACG TGGAACCATC ACCCTAATCA AGTTTTTTTG GGGGGTCGAG GGTGCCGGTA 1081 AGGCACTAAT CGATCCTTAA GGGAAGACCC CGATTTTAGA AGCTTTGTG //
Primer: M13_-40 Sequence file: RDB15497_A7K9d.seq
>01269_15479_A7K9_M13_-40_B02_02_ABI08.ab1 1 AAAGCAAGTG AATTGGTAAT ACGACTCACT ATAGGGCGAA TTGGAGCTCC ACCGCGGTGG 61 CGGCCGCTCT AGAACTAGTG GATCCCCCGG GCTGCAGGAA TTCCTTTTTT TTTTTTTTTT 121 TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT TTTTTTTTTT 181 TTTTTTTTTT TTTTTTTTTA AAAACCCCCT TTTTTTTTTT TGGGACGGGG ATTGGGGCCC 241 CAAAAAGGGG CCGGGAAGGG GGGAGGGGAG GGCACCCCCC CCCCAAACCC TGGGAGCCCA 301 AAAAGGACCC CCCCCCAGGG GGCAAAACCC CCCCCCGGGG ACACACAATT TTTTCCCTAA 361 AAAACAGGGG GAGGGGCAGG GGGAACCTGG CAACAGGGAG GGCATGGGGG GCCCCCGGGA 421 CCCCCCCCCC CCCCAGGGCC TGAACTCCGG GAAAGTGGAA AAACAGGGGA GGTTGGGAAG 481 GGCCCCCAGG GACCCCCGGC CCCTACCCAA GGGGCCCCCC CCCAGGGGGA AGGGGGTCCC 541 CCTCTTCCTG GAACCCCCCC AGGGAACCCC CCCCGGCCTG GGGGGTTTCC CACCCCTCAA 601 TGGCCCCCGT GTACGGGTCA AAATTTCCGA AAAAGGGGCC CTGGATGCCT GAAGGATAAC 661 TTCTGAACTT GGCCTTGGCG TCACTGAAAC TCTCAAACAC AAAATAAACT GACTGGTACC 721 TCTGGCCCTG GGAGGGTGCC CCCCCCCCCC TCGGGTCAAA AGCCCAATTC CGGCTCCTCA 781 AAAAGGGAGT GCGGAACTCC CCGAAAGAGA GACACAGCCC GCACCATAGC CTTCACTCCC 841 CGTCTGTTTT ACACGCCCGA AATTCCCGGG ACCAGTACGC GTGAACGCTT CCTATATTCC 901 CTATCACGAG GCCTCAGGGA CGCCAGCAAT GTCTTGCGAA ACTGCGCGAA GGTGCGGTCG 961 CCACATGGAC ACGTGCTCAA CACTCTGGTC ACACTCGGGT AAGGATCATG GCGAGACGTG 1021 CGTATATACT GTGCATGTAC CCAGAGCTCA AGCTGTCAGA GTCGCGACAG AAGTCTGAAT 1081 CCT //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Kaneda, N., Isolation of a novel cDNA clone for human tyrosine hydroxylase: alternative RNA splicing produces four kinds of mRNA from a single gene. Biochem. Biophys. Res. Commun., 146, 971-975 (1987). PMID 2887169.
user_report	Takekoshi K., Stimulation of catecholamine biosynthesis via the protein kinase C pathway by endothelin-1 in PC12 rat pheochromocytoma cells. Biochem. Pharmacol., 63, 977-984 (2002). PMID 11911850.
user_report	Takekoshi K., Ca(2+) mobilization, tyrosine hydroxylase activity, and signaling mechanisms in cultured porcine adrenal medullary chromaffin cells: effects of leptin. Endocrinology, 142, 290-298 (2001). PMID 11145592.
user_report	Takekoshi K., Leptin stimulates catecholamine synthesis in a PKC-dependent manner in cultured porcine adrenal medullary chromaffin cells. Endocrinology, 142, 4861-4871 (2001). PMID 11606454.
user_report	Takekoshi K., Effects of natriuretic peptides (ANP, BNP, CNP) on catecholamine synthesis and TH mRNA levels in PC12 cells. Life Sci., 66, PL303-311 (2000). PMID 10834306.
user_report	Takekoshi K., kappa-Opioid inhibits catecholamine biosynthesis in PC12 rat pheochromocytoma cell. FEBS Lett., 477, 273-277 (2000). PMID 10908734.
user_report	Isobe K., Expression of mRNA coding for four catecholamine-synthesizing enzymes in human adrenal pheochromocytomas. Eur. J. Endocrinol., 138, 383-387 (1998). PMID 9578504.
user_report	Yamakuni T., A novel protein containing Cdc10/SWI6 motifs regulates expression of mRNA encoding catecholamine biosynthesizing enzymes. J. Biol. Chem., 273, 27051-27054 (1998). PMID 9765218.
user_report	Isobe K., Pituitary adenylate cyclase-activating polypeptide induces gene expression of the catecholamine synthesizing enzymes, tyrosine hydroxylase and dopamine beta hydroxylase, through 3',5'-cyclic adenosine monophosphate- and protein kinase C-dependent mechanisms in cultured porcine adrenal medullary chromaffin cells. Neuropeptides., 30, 167-175 (1996). PMID 8771559.
user_report	Isobe K., Expression of mRNAs coding for catecholamine synthesizing enzymes in human adrenal pheochromocytoma. Jpn. J. Clin. Pathol., 43, 540-544 (1995). PMID 7602797.

2023.05.02

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