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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pg DQ1B

DQw6B, pg DQ6B, DQB1*0601, HLA- DQB1 (genomic DNA+cDNA).

Catalog number RDB01228
Resource name pg DQ1B
Alternative name DQw6B, pg DQ6B, DQB1*0601
Clone info. Human HLA-DQB1 genomic DNA+cDNA from EBV-B cells TOK/AKIBA libraries, HLA-DQB1.
Vector backbone pTZ18R (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Human HLA-DQB1 Genomic DNA + cDNA
Depositor|Developer Kimura, Akinori |
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External Database
Human HLA-DQB1

          Reference sequence
            
           

          Distribution information

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          RDB01228 pg DQ1B DNA solution

          Please review the QC test results indicated by check icon below as well as clone information before placing your order.

          How to cite this biological resource

          Materials & Methods section:

          The pg DQ1B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01228).

          Reference section:

          Nishimura, Y., Iwanaga, T., Inamitsu, T., Yanagawa, Y., Yasunami, M., Kimura, A., Hirokawa, K., Sasazuki, T., Expression of the human MHC, HLA-DQW6 genes alters the immune response in C57BL/6 mice. J. Immunol., 145, 353-360 (1990). PMID 2358677.

          Further references such as user reports and related articles (go to bottom)


          References and tips

          Featured content

          Featured content HLA Clones (en) (English text)
          Featured content HLA Clones (ja) (Japanese text)

          QC test results

          RIKEN BRC has sequenced portions of this material for quality test.
          Please review the QC test results indicated by check icon as well as clone information before placing your order.

          Test sheet RDB01228_B1Bfp1-2.pdf check

          Nucleotide sequence of a portion of this resource (if available).

          Primer: pUC-CAPbs_up_F2 (Pr0739)
          Region: vector junction seq.,insert 5'
          Sequence file: RDB01228_B1Bfa.seq check
          >01228_01228_B1Bf_2_pUC-CAPbs_up_F2_F01_16_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGNTTNNN NCTTTATGCT TCCGGCTCGT ATGTTGTGTG
             61 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACATGATT ACGAATTTAA
            121 TACGACTCAC TATAGGGAAT TCCACCAGAG GTACAAAGAA GAACTGGTAC CATTTCTTCT
            181 GAAGCTATTC CAAGTAATTG AAAAGGAGGA CTCCTCTCTA ACTCATTTTA TGAGGCCAGC
            241 GTCATCCTGA TACCAAAGCC TGACAGAGAT ACAACAAAAA AGGAAAACTT CAGGCCAATA
            301 TCACTGATGG ACACTGATTA AAAAATCCTC AATAAAATAC TGGCAAGCCG AATCCACCAG
            361 CACATCAAAA AGTTCATCCA CCACGATCAA GTTGGCATCA TCCCCAGGAT GCCAGGCTGT
            421 TTCAACATAT GCAAATCAAT AAACATAATT CATTACATAA ACAGAATTAA AGACAAAAAC
            481 CACATGATTA TCTCAATAGA CACAGAAAAG GCCTTCAATA AAATTAAACA TCCCTTCATG
            541 TTAAAAACTC TCAATAAACT AGGTATTGAA GGAACATACC TCAAAATAAT AAGAATCACT
            601 TATGACNAGC CCACAGACNA TATCATACTG AATGGGCAAA AGCCANAANC ATTCCCCTTA
            661 AAACNTGNNN AAACAACNAN GNCCNCTCNC NNNCTCCNNT TCNNCANGNN ATNNNNNNTN
          721 CNGNNNNNNN NNN
          //
          Primer: pUC-M13_-100 (Pr0310)
          Region: insert 3',vecter junction seq.
          Sequence file: RDB01228_B1Bfb.seq check
          >01228_01228_B1Bf_2_pUC-M13_-100_E01_13_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NNNNNNTTCC CNNNNNCNAC GTTGTAAAAC
             61 GACGGCCAGT GCCNAGCNTG NNNNNNNGCA GTGATTTCCT GCCTCTTCTC AATTAAGAAA
            121 GTTCAAAATA CAGATAACTC AGGATCATGT TTAATTATGT AAAAAAGCTC TAAAGTCAGG
            181 TAATGTTTTT CATGTGCTTC TCTTGAGCAG TCTGAGGAGA GAATAGAAAC AGAAACCCCT
            241 TGGGACCTGA GTAGACGCAG CTGGCCGTGC ACAGGCAGAG GCTCTGGGTC AGTGCAGGAA
            301 GCAGAGTCAC AGCCAGCGCC TTGGGGTGGG GATGAAAGGA GATGACCTGG TGGCTGCGTG
            361 ACAGCCACTG TAGGACTTTG ATCTCGGGGG ACAAGCTGAC ACAGGCAGCT GGGAATTCTG
            421 GGCAGGCACA AGCAGGCATC ACAGAAGAGT GATAACCAAT CCTAGTTAAA ATAGTCTCAG
            481 GAGTCAGTGC AGAAGCCCTG GAGAAGAGAG AAGAGGAGTG ATCAGCACAG GGTATGCTGG
            541 CGGGCCTGCC ATCTCCCCCA CCCCTCANGG GCCCCCTGCA GCTTCNGACA GAGAAAGNTG
            601 ANGNCCAGGG TATATCATCN TCACCTCCCC TNANNTCTGN GCAAAGANGA AATCNGCTCA
            661 TGNNNNNCNG AANTTCNNCT TGNTGNNNAN GTGNGGNANN NNGGGNNCAN CTGNNNNNCN
          721 NCNNGGNNNN NNNNNN
          //
          Primer: HLA00643-M_F2 (Pr0974)
          Region: insert mid
          Sequence file: RDB01228_B1Bfc.seq check
          >01228_01228_B1Bf_2_HLA00643-M_F2_F03_18_ABI24.ab1
              1 NNNNNNNNNN NNNNNNNNNN NNNGTCTGCC NNGANNCNGA TGAGGTCCTT CAGCTCCNGT
             61 GCTGATTGGT TCTTTTCCAA AGGCCCATCT AATCCTACCA CGCACGGAAA TATCCACAGG
            121 TTTTTATTCT TTCTGCCAGC TACATCAGAT CCATCAGGTC CGAGCTGTGT TGACTACCAC
            181 TACTTTTCCC TTTGTCTCAA TTATGTCTTG GAAGAAGGCT TTGCGGATCC CCGGAGGCCT
            241 TCGGGCACCA ACTGTGACCT TGATGCTGGC GATGCTGAGC ACCCCAGTGG CTGAGGGCAG
            301 AGACCCTCCC GGTAAGTGCA GGGCAGCTGC TCTCCAGAGC CGCCACTCTG GGAACAGGCA
            361 TTCCTTGGGC TGGGGTAGGG GGATGGTGAT CTCCATGATC TCGGACACAA TCTTTCATCA
            421 ACATTTCCTC TCTTTTGGGA AAGAAAGCTA TGTTGCATTC CCATTTATCT TTTAGTGATG
            481 AGGTGAGGAC AATCCAATCC CCATCCTACA GGCTTAAGCC TGGAAGAAGA GGAGAGAGGA
            541 GAGGAAAGAG GAGATAAAGT GTACATTTAC TACCAGTGAT AGGACAAAGT GAGCATGGGA
            601 NTANTTTTGA AAATATGAAT TTCTCCNAAG ACACAGCANG NNTTGCCGTT TNNNGTGCCC
          661 CNANANTTTG NCNNNNNTAA ATANNANGGN NNNNNGNNNN NNNNANNNNN NNNNN
          //
          Primer: HLA00643-M_F (Pr0969)
          Region: insert mid
          Sequence file: RDB01228_B1Bfd.seq check
          >01228_01228_B1Bf_2_HLA00643-M_F_D03_12_ABI24.ab1
              1 TNNNNNNNNN NNNCNNNCCT GNNAGTTACN CNTAACNGTG GAGGAGCCTT TTGTAATATT
             61 AAACAAGACA AATTAATCTT AAATTTGTAT ATGTGAAGAT CTAGATGTAA AATGCATGAA
            121 ACATGACCCA CATTTTACAA AGAGGAGCCT GGGGCAAAAA TAAATTCAGT AATTTGTTGA
            181 CTCTCCTAAA GCACATTAGT GGTGGAACTG CAACTCACCA TTAATTCCTT CTAAGAACTT
            241 TGCTCTTCTC ACCAAAACTT AAGGCTCTTC AGCGTGTCTA AGACAACAGC AGTAAAAATT
            301 TCTATGACAG CAATTTTCTC TCCCCTGAAA TATGATCCCC ACTTAATTTG CTTTATTAAA
            361 AATCCCAAGT ATAATAACAA CTGGTTTTTA ATCAATATTA CAGAGATGTT TACTGTTGAA
            421 TCGCATTTTT CTTTTGCCTC TCAAAATCCC TGAGGCACTC ATTCTTCAGC TTTTCTATAA
            481 TTGAGAGAAA ATTTTCACCT CAAATGTTCA TCCAGTGCAA TTTGAAGACA TCACAGTGCC
            541 AGGCACTGGA CTGAGAACCT TCACCNAAAA AATGTCTGCC CAGAGACAGA NGANGNCCTT
            601 CAGCTCCNGT GCTGANTGGN TCTTTTCCNA AGGCCNNTCT AATCCNANNN CGCNCGGNAA
            661 TANCCNCNGG NTTTTANNCN TNNNGNCNNN NNNNTNNNNN NCNNNNNNNN ANNNGNNNNN
          721 ANNNNNNNTN NNTTNNCNNT NNNNNNNNNN
          //

          Please visit Sequencing and PCR primers for primer information.


          References

          Original, user report and related articles

          original Nishimura, Y., Expression of the human MHC, HLA-DQW6 genes alters the immune response in C57BL/6 mice. J. Immunol., 145, 353-360 (1990). PMID 2358677.

          2023.05.01

          GNP_filter3_RDBDEP_html_230501.pl