RDB01228 - pg DQ1B

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pg DQ1B (#RDB01228)

DQw6B, pg DQ6B, DQB1*0601, HLA- DQB1 (genomic DNA+cDNA).

Alternative name	DQw6B, pg DQ6B, DQB1*0601
Clone info.	Human HLA-DQB1 genomic DNA+cDNA from EBV-B cells TOK/AKIBA libraries, HLA-DQB1.
Vector backbone	pTZ18R (Plasmid)
Size of vector backbone	2.9 kb
Selectable markers	Amp^r
Gene/insert name	Human HLA-DQB1 Genomic DNA + cDNA
Depositor\|Developer	Kimura, Akinori \|

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Terms and conditions for distribution For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.

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Ordering forms	Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] MTA, for use for for-profit purpose [Word] Please visit Information of Request for Distribution.[link]
Terms and conditions for distribution	For use of the BIOLOGICAL RESOURCE by a for-profit organization, the RECIPIENT must reach agreement on terms and conditions of use of it with DEPOSITOR and must obtain a prior written consent from the DEPOSITOR. In publishing the research results to be obtained by use of the BIOLOGICAL RESOURCE, an acknowledgment to the DEPOSITOR is requested.
Remarks	((Additional form)) Approval Form(FormD)

必要書類	提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 提供同意書 (MTA, 営利目的用)[Word] 手続きの概要は、「提供申込みについて[link]」をご覧ください。
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Catalog #	Resource name	Shipping form	Fee (non-profit org.)
RDB01228	pg DQ1B	DNA solution	JPY 9,460 (not-for-profit academic purpose) JPY 18,920 (for-profit-research purpose) plus cost of shipping containers, dry ice (if required) and shipping charge

Please review the QC test results indicated by check

icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The pg DQ1B was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01228).

Reference section:

Nishimura, Y., Iwanaga, T., Inamitsu, T., Yanagawa, Y., Yasunami, M., Kimura, A., Hirokawa, K., Sasazuki, T., Expression of the human MHC, HLA-DQW6 genes alters the immune response in C57BL/6 mice. J. Immunol., 145, 353-360 (1990). PMID 2358677.

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet	RDB01228_B1Bfp1-2.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F2 (Pr0739) Region: vector junction seq.,insert 5' Sequence file: RDB01228_B1Bfa.seq
>01228_01228_B1Bf_2_pUC-CAPbs_up_F2_F01_16_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGNTTNNN NCTTTATGCT TCCGGCTCGT ATGTTGTGTG 61 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACATGATT ACGAATTTAA 121 TACGACTCAC TATAGGGAAT TCCACCAGAG GTACAAAGAA GAACTGGTAC CATTTCTTCT 181 GAAGCTATTC CAAGTAATTG AAAAGGAGGA CTCCTCTCTA ACTCATTTTA TGAGGCCAGC 241 GTCATCCTGA TACCAAAGCC TGACAGAGAT ACAACAAAAA AGGAAAACTT CAGGCCAATA 301 TCACTGATGG ACACTGATTA AAAAATCCTC AATAAAATAC TGGCAAGCCG AATCCACCAG 361 CACATCAAAA AGTTCATCCA CCACGATCAA GTTGGCATCA TCCCCAGGAT GCCAGGCTGT 421 TTCAACATAT GCAAATCAAT AAACATAATT CATTACATAA ACAGAATTAA AGACAAAAAC 481 CACATGATTA TCTCAATAGA CACAGAAAAG GCCTTCAATA AAATTAAACA TCCCTTCATG 541 TTAAAAACTC TCAATAAACT AGGTATTGAA GGAACATACC TCAAAATAAT AAGAATCACT 601 TATGACNAGC CCACAGACNA TATCATACTG AATGGGCAAA AGCCANAANC ATTCCCCTTA 661 AAACNTGNNN AAACAACNAN GNCCNCTCNC NNNCTCCNNT TCNNCANGNN ATNNNNNNTN 721 CNGNNNNNNN NNN //
Primer: pUC-M13_-100 (Pr0310) Region: insert 3',vecter junction seq. Sequence file: RDB01228_B1Bfb.seq
>01228_01228_B1Bf_2_pUC-M13_-100_E01_13_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NNNNNNTTCC CNNNNNCNAC GTTGTAAAAC 61 GACGGCCAGT GCCNAGCNTG NNNNNNNGCA GTGATTTCCT GCCTCTTCTC AATTAAGAAA 121 GTTCAAAATA CAGATAACTC AGGATCATGT TTAATTATGT AAAAAAGCTC TAAAGTCAGG 181 TAATGTTTTT CATGTGCTTC TCTTGAGCAG TCTGAGGAGA GAATAGAAAC AGAAACCCCT 241 TGGGACCTGA GTAGACGCAG CTGGCCGTGC ACAGGCAGAG GCTCTGGGTC AGTGCAGGAA 301 GCAGAGTCAC AGCCAGCGCC TTGGGGTGGG GATGAAAGGA GATGACCTGG TGGCTGCGTG 361 ACAGCCACTG TAGGACTTTG ATCTCGGGGG ACAAGCTGAC ACAGGCAGCT GGGAATTCTG 421 GGCAGGCACA AGCAGGCATC ACAGAAGAGT GATAACCAAT CCTAGTTAAA ATAGTCTCAG 481 GAGTCAGTGC AGAAGCCCTG GAGAAGAGAG AAGAGGAGTG ATCAGCACAG GGTATGCTGG 541 CGGGCCTGCC ATCTCCCCCA CCCCTCANGG GCCCCCTGCA GCTTCNGACA GAGAAAGNTG 601 ANGNCCAGGG TATATCATCN TCACCTCCCC TNANNTCTGN GCAAAGANGA AATCNGCTCA 661 TGNNNNNCNG AANTTCNNCT TGNTGNNNAN GTGNGGNANN NNGGGNNCAN CTGNNNNNCN 721 NCNNGGNNNN NNNNNN //
Primer: HLA00643-M_F2 (Pr0974) Region: insert mid Sequence file: RDB01228_B1Bfc.seq
>01228_01228_B1Bf_2_HLA00643-M_F2_F03_18_ABI24.ab1 1 NNNNNNNNNN NNNNNNNNNN NNNGTCTGCC NNGANNCNGA TGAGGTCCTT CAGCTCCNGT 61 GCTGATTGGT TCTTTTCCAA AGGCCCATCT AATCCTACCA CGCACGGAAA TATCCACAGG 121 TTTTTATTCT TTCTGCCAGC TACATCAGAT CCATCAGGTC CGAGCTGTGT TGACTACCAC 181 TACTTTTCCC TTTGTCTCAA TTATGTCTTG GAAGAAGGCT TTGCGGATCC CCGGAGGCCT 241 TCGGGCACCA ACTGTGACCT TGATGCTGGC GATGCTGAGC ACCCCAGTGG CTGAGGGCAG 301 AGACCCTCCC GGTAAGTGCA GGGCAGCTGC TCTCCAGAGC CGCCACTCTG GGAACAGGCA 361 TTCCTTGGGC TGGGGTAGGG GGATGGTGAT CTCCATGATC TCGGACACAA TCTTTCATCA 421 ACATTTCCTC TCTTTTGGGA AAGAAAGCTA TGTTGCATTC CCATTTATCT TTTAGTGATG 481 AGGTGAGGAC AATCCAATCC CCATCCTACA GGCTTAAGCC TGGAAGAAGA GGAGAGAGGA 541 GAGGAAAGAG GAGATAAAGT GTACATTTAC TACCAGTGAT AGGACAAAGT GAGCATGGGA 601 NTANTTTTGA AAATATGAAT TTCTCCNAAG ACACAGCANG NNTTGCCGTT TNNNGTGCCC 661 CNANANTTTG NCNNNNNTAA ATANNANGGN NNNNNGNNNN NNNNANNNNN NNNNN //
Primer: HLA00643-M_F (Pr0969) Region: insert mid Sequence file: RDB01228_B1Bfd.seq
>01228_01228_B1Bf_2_HLA00643-M_F_D03_12_ABI24.ab1 1 TNNNNNNNNN NNNCNNNCCT GNNAGTTACN CNTAACNGTG GAGGAGCCTT TTGTAATATT 61 AAACAAGACA AATTAATCTT AAATTTGTAT ATGTGAAGAT CTAGATGTAA AATGCATGAA 121 ACATGACCCA CATTTTACAA AGAGGAGCCT GGGGCAAAAA TAAATTCAGT AATTTGTTGA 181 CTCTCCTAAA GCACATTAGT GGTGGAACTG CAACTCACCA TTAATTCCTT CTAAGAACTT 241 TGCTCTTCTC ACCAAAACTT AAGGCTCTTC AGCGTGTCTA AGACAACAGC AGTAAAAATT 301 TCTATGACAG CAATTTTCTC TCCCCTGAAA TATGATCCCC ACTTAATTTG CTTTATTAAA 361 AATCCCAAGT ATAATAACAA CTGGTTTTTA ATCAATATTA CAGAGATGTT TACTGTTGAA 421 TCGCATTTTT CTTTTGCCTC TCAAAATCCC TGAGGCACTC ATTCTTCAGC TTTTCTATAA 481 TTGAGAGAAA ATTTTCACCT CAAATGTTCA TCCAGTGCAA TTTGAAGACA TCACAGTGCC 541 AGGCACTGGA CTGAGAACCT TCACCNAAAA AATGTCTGCC CAGAGACAGA NGANGNCCTT 601 CAGCTCCNGT GCTGANTGGN TCTTTTCCNA AGGCCNNTCT AATCCNANNN CGCNCGGNAA 661 TANCCNCNGG NTTTTANNCN TNNNGNCNNN NNNNTNNNNN NCNNNNNNNN ANNNGNNNNN 721 ANNNNNNNTN NNTTNNCNNT NNNNNNNNNN //

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original	Nishimura, Y., Expression of the human MHC, HLA-DQW6 genes alters the immune response in C57BL/6 mice. J. Immunol., 145, 353-360 (1990). PMID 2358677.

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human HLA-DQB1

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Human HLA-DQB1

Reference sequence