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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

pg DQ1B

DQw6B, pg DQ6B, DQB1*0601, HLA- DQB1 (genomic DNA+cDNA).

Catalog number RDB01228
Resource name pg DQ1B
Alternative name DQw6B, pg DQ6B, DQB1*0601
Clone info. Human HLA-DQB1 genomic DNA+cDNA from EBV-B cells TOK/AKIBA libraries, HLA-DQB1.
Vector backbone pTZ18R (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Human HLA-DQB1 Genomic DNA + cDNA
Depositor|Developer Kimura, Akinori |
 
Other clones in our bank human HLA-DQB1 (NCBI Gene 3119) |

Distribution information

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Terms and conditions set forth by the DEPOSITOR This material is provided with written consent of Depositor.
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RDB01228 pg DQ1B DNA solution

Ordering Information [in Japanese] [in English]
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

References and tips

Electronic file

Original reference

original Nishimura, Y., Expression of the human MHC, HLA-DQW6 genes alters the immune response in C57BL/6 mice. J. Immunol., 145, 353-360 (1990). PMID 2358677.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content HLA Clones (en) (English text)
Featured content HLA Clones (ja) (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB01228_B1Bfp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-CAPbs_up_F2
Region: vector junction seq.,insert 5'
Sequence file: RDB01228_B1Bfa.seq check
>01228_01228_B1Bf_2_pUC-CAPbs_up_F2_F01_16_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGNTTNNN NCTTTATGCT TCCGGCTCGT ATGTTGTGTG
   61 GAATTGTGAG CGGATAACAA TTTCACACAG GAAACAGCTA TGACATGATT ACGAATTTAA
  121 TACGACTCAC TATAGGGAAT TCCACCAGAG GTACAAAGAA GAACTGGTAC CATTTCTTCT
  181 GAAGCTATTC CAAGTAATTG AAAAGGAGGA CTCCTCTCTA ACTCATTTTA TGAGGCCAGC
  241 GTCATCCTGA TACCAAAGCC TGACAGAGAT ACAACAAAAA AGGAAAACTT CAGGCCAATA
  301 TCACTGATGG ACACTGATTA AAAAATCCTC AATAAAATAC TGGCAAGCCG AATCCACCAG
  361 CACATCAAAA AGTTCATCCA CCACGATCAA GTTGGCATCA TCCCCAGGAT GCCAGGCTGT
  421 TTCAACATAT GCAAATCAAT AAACATAATT CATTACATAA ACAGAATTAA AGACAAAAAC
  481 CACATGATTA TCTCAATAGA CACAGAAAAG GCCTTCAATA AAATTAAACA TCCCTTCATG
  541 TTAAAAACTC TCAATAAACT AGGTATTGAA GGAACATACC TCAAAATAAT AAGAATCACT
  601 TATGACNAGC CCACAGACNA TATCATACTG AATGGGCAAA AGCCANAANC ATTCCCCTTA
  661 AAACNTGNNN AAACAACNAN GNCCNCTCNC NNNCTCCNNT TCNNCANGNN ATNNNNNNTN
721 CNGNNNNNNN NNN
//
Primer: pUC-M13_-100
Region: insert 3',vecter junction seq.
Sequence file: RDB01228_B1Bfb.seq check
>01228_01228_B1Bf_2_pUC-M13_-100_E01_13_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNNNNNGCN NNNNNNTTCC CNNNNNCNAC GTTGTAAAAC
   61 GACGGCCAGT GCCNAGCNTG NNNNNNNGCA GTGATTTCCT GCCTCTTCTC AATTAAGAAA
  121 GTTCAAAATA CAGATAACTC AGGATCATGT TTAATTATGT AAAAAAGCTC TAAAGTCAGG
  181 TAATGTTTTT CATGTGCTTC TCTTGAGCAG TCTGAGGAGA GAATAGAAAC AGAAACCCCT
  241 TGGGACCTGA GTAGACGCAG CTGGCCGTGC ACAGGCAGAG GCTCTGGGTC AGTGCAGGAA
  301 GCAGAGTCAC AGCCAGCGCC TTGGGGTGGG GATGAAAGGA GATGACCTGG TGGCTGCGTG
  361 ACAGCCACTG TAGGACTTTG ATCTCGGGGG ACAAGCTGAC ACAGGCAGCT GGGAATTCTG
  421 GGCAGGCACA AGCAGGCATC ACAGAAGAGT GATAACCAAT CCTAGTTAAA ATAGTCTCAG
  481 GAGTCAGTGC AGAAGCCCTG GAGAAGAGAG AAGAGGAGTG ATCAGCACAG GGTATGCTGG
  541 CGGGCCTGCC ATCTCCCCCA CCCCTCANGG GCCCCCTGCA GCTTCNGACA GAGAAAGNTG
  601 ANGNCCAGGG TATATCATCN TCACCTCCCC TNANNTCTGN GCAAAGANGA AATCNGCTCA
  661 TGNNNNNCNG AANTTCNNCT TGNTGNNNAN GTGNGGNANN NNGGGNNCAN CTGNNNNNCN
721 NCNNGGNNNN NNNNNN
//
Primer: HLA00643-M_F2
Region: insert mid
Sequence file: RDB01228_B1Bfc.seq check
>01228_01228_B1Bf_2_HLA00643-M_F2_F03_18_ABI24.ab1
    1 NNNNNNNNNN NNNNNNNNNN NNNGTCTGCC NNGANNCNGA TGAGGTCCTT CAGCTCCNGT
   61 GCTGATTGGT TCTTTTCCAA AGGCCCATCT AATCCTACCA CGCACGGAAA TATCCACAGG
  121 TTTTTATTCT TTCTGCCAGC TACATCAGAT CCATCAGGTC CGAGCTGTGT TGACTACCAC
  181 TACTTTTCCC TTTGTCTCAA TTATGTCTTG GAAGAAGGCT TTGCGGATCC CCGGAGGCCT
  241 TCGGGCACCA ACTGTGACCT TGATGCTGGC GATGCTGAGC ACCCCAGTGG CTGAGGGCAG
  301 AGACCCTCCC GGTAAGTGCA GGGCAGCTGC TCTCCAGAGC CGCCACTCTG GGAACAGGCA
  361 TTCCTTGGGC TGGGGTAGGG GGATGGTGAT CTCCATGATC TCGGACACAA TCTTTCATCA
  421 ACATTTCCTC TCTTTTGGGA AAGAAAGCTA TGTTGCATTC CCATTTATCT TTTAGTGATG
  481 AGGTGAGGAC AATCCAATCC CCATCCTACA GGCTTAAGCC TGGAAGAAGA GGAGAGAGGA
  541 GAGGAAAGAG GAGATAAAGT GTACATTTAC TACCAGTGAT AGGACAAAGT GAGCATGGGA
  601 NTANTTTTGA AAATATGAAT TTCTCCNAAG ACACAGCANG NNTTGCCGTT TNNNGTGCCC
661 CNANANTTTG NCNNNNNTAA ATANNANGGN NNNNNGNNNN NNNNANNNNN NNNNN
//
Primer: HLA00643-M_F
Region: insert mid
Sequence file: RDB01228_B1Bfd.seq check
>01228_01228_B1Bf_2_HLA00643-M_F_D03_12_ABI24.ab1
    1 TNNNNNNNNN NNNCNNNCCT GNNAGTTACN CNTAACNGTG GAGGAGCCTT TTGTAATATT
   61 AAACAAGACA AATTAATCTT AAATTTGTAT ATGTGAAGAT CTAGATGTAA AATGCATGAA
  121 ACATGACCCA CATTTTACAA AGAGGAGCCT GGGGCAAAAA TAAATTCAGT AATTTGTTGA
  181 CTCTCCTAAA GCACATTAGT GGTGGAACTG CAACTCACCA TTAATTCCTT CTAAGAACTT
  241 TGCTCTTCTC ACCAAAACTT AAGGCTCTTC AGCGTGTCTA AGACAACAGC AGTAAAAATT
  301 TCTATGACAG CAATTTTCTC TCCCCTGAAA TATGATCCCC ACTTAATTTG CTTTATTAAA
  361 AATCCCAAGT ATAATAACAA CTGGTTTTTA ATCAATATTA CAGAGATGTT TACTGTTGAA
  421 TCGCATTTTT CTTTTGCCTC TCAAAATCCC TGAGGCACTC ATTCTTCAGC TTTTCTATAA
  481 TTGAGAGAAA ATTTTCACCT CAAATGTTCA TCCAGTGCAA TTTGAAGACA TCACAGTGCC
  541 AGGCACTGGA CTGAGAACCT TCACCNAAAA AATGTCTGCC CAGAGACAGA NGANGNCCTT
  601 CAGCTCCNGT GCTGANTGGN TCTTTTCCNA AGGCCNNTCT AATCCNANNN CGCNCGGNAA
  661 TANCCNCNGG NTTTTANNCN TNNNGNCNNN NNNNTNNNNN NCNNNNNNNN ANNNGNNNNN
721 ANNNNNNNTN NNTTNNCNNT NNNNNNNNNN
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Nishimura, Y., Expression of the human MHC, HLA-DQW6 genes alters the immune response in C57BL/6 mice. J. Immunol., 145, 353-360 (1990). PMID 2358677.

2021.04.21

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