Resource data sheet
DNA Bank Top
Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Chicken N-cadherin full length cDNA (#RDB01187)

Chicken N-cadherin

Alternative name Chicken N-cadherin cDNA
Clone info. Chicken N-cadherin cDNA from chicken embryonic brain and retina cDNA library
Vector backbone pBluescript SK (+) (Plasmid)
Size of vector backbone 3.0 kb
Selectable markers Amp^r
Gene/insert name Chicken cadherin 2, type 1, N-cadherin (neuronal) cDNA
Depositor|Developer Takeichi, Masatoshi |
 
Additional map

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms
Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ]
MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ]
Approval form by depositor [Word]
Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. 
Terms and conditions for distribution This material is provided with written consent of Depositor.
Remarks ((Additional form)) Approval Form(FormD)
Please obtain written approval of the depositor.
提供案内 (日本国内) [open/close]

必要書類
提供依頼書  [依頼書の記入例 ]
提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ]
寄託者による提供承諾書 [Word]
手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。
MTAに書く使用条件 寄託者に許可をとること。
備考 【追加提供依頼書】承諾書(FormD)
寄託者の書面による提供承諾が必要です。

Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01187 Chicken N-cadherin full length cDNA DNA solution Please contact us.

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Chicken N-cadherin full length cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01187).

Reference section:

Hatta, K., Nose, A., Nagafuchi, A., Takeichi, M., Cloning and expression of cDNA encoding a neural calcium-dependent cell adhesion molecule: its identity in the cadherin gene family. J. Cell Biol., 106, 873- 881 (1988). PMID 2831236. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)


QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB12824_A4Gg.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: M13_-40
Sequence file: RDB12824_A4Gga.seq check
>01187_12824_A4Gg_M13(-40)_C07_07.ab1
    1 CAGCCGCGAT TGTAATACGA CTCACTATAG GGCGAATTGG GTACCGGGCC CCCCCTCGAG
   61 GTCGACGGTA TCGATAAGCT TGATATCGAA TTCGGGCCAT GTGCCGGATA GCGGGAACGC
  121 CGCCGCGGAT CCTGCCGCCG CTGGCGCTGA TGCTGCTGGC GGCCCTGCAG CAGGCACCGA
  181 TAAAAGCAAC TTGTGAAGAC ATGTTGTGCA AGATGGGATT TCCTGAAGAT GTGCACAGTG
  241 CAGTCGTGTC GAGGAGTGTA CATGGAGGAC AACCTCTGCT CAATGTGAGG TTTCAAAGCT
  301 GCGATGAAAA CAGAAAAATA TACTTTGGAA GCAGTGAGCC AGAAGATTTT AGAGTAGGTG
  361 AAGATGGTGT GGTATATGCA GAGAGAAGCT TTCAACTTTC AGCAGAGCCC ACGGAGTTTG
  421 TAGTGTCTGC TCGAGACAAG GAAACTCAGG AAGAATGGCA AATGAAGGTG AAGCTAACCC
  481 CTGAACCAGC ATTCACAGGG GCCTCAGAAA AGGACCAAAA GAAAATTGAA GACATCATAT
  541 TTCCATGGCA ACAATATAAG GACAGCAGCC ATCTGAAGAG ACAGAAGAGA GACTGGGTTA
  601 TCCCTCCAAT CAACCTACCA GAAAATTCCA GAGGACCTTT TCCTCAAGAA TTAGTTAGGA
  661 TTCGGTCTGA TCGTGATAAA AGCCTTTCGC TACGGTACAG TGTGACTGGC CCAGGAGCTG
  721 ACCAACCTCC AACAGGAATC TTCATCATCA ACCCCATCTC AGGACAGCTG TCTGTGACAA
  781 AGCCTTTAGA TCGGGAGCAG ATTGCTTCTT TTCATCTGAG AGCACATGCA GTGGATGTAA
  841 ATGGAAACCA GGTGGAAAAT CCTATTGATA TTGTGATTAA CGTCATTGAT ATGAATGATA
  901 ACAGACCTGA ATTCTTGCAT CAGGTTTGGA ATGGGACAGT TCCTGAAGGA TCAAAGCCAG
  961 GAACCCTATG TAATGACTGT TACTGCCATC GATGCTGATG ATCCCAAATG CACAGAATGG
 1021 GATGCTGAGA TACAGAAATC TTTGTCACAG GCACCCAAAG CAGTCCCCTC TCCCCAACAT
 1081 GTTTACAATC CAACCAATGA GACCTTGATT GACATAATAA CGTAGCCAGC TGGGCTCGAA
1141 CGGAGAGGAA AGGTTACAAC C
//
Primer: Reverse2
Sequence file: RDB12824_A4Ggb.seq check
>01187_12824_A4Gg_Reverse2_D07_10.ab1
    1 AATGGACGGG GATTCGCCAA GGCTCGAATT AACCCTCACT AAAGGGCAAC AAAAGCTGGA
   61 GCTCCACCGC GGTGGCGGCC GCTCTAGAAC TAGTGGATCC CCCGGGCTGC AGGAATTCGG
  121 GCCTTATTTT AATAAAAGCA ATGCGATGTA ACAAAAGCAT ATTGAAGCGC ATTGTGATTC
  181 ACCTTACTCC TCCCCACCCC AACATATCTT GTCTCTTTAA TGCATCATTT CATAAATCAG
  241 TACCATTAAA GCCTTAAACA TAAAACTAAT TCCGATCTGA AAAAGGTACA AAAAGGCACA
  301 TAAAATCCCA GTGCTTCTGT ACTGTAAAAT TCAAGTGTAA CTGAGCTCAA TATTTTTTTC
  361 CAAACAGCAT TGGATCACTG ATATTCCACG GAGCCCAAAT TGGTTTGCAG CCTATGCCAA
  421 AGCCTTCAGC AAGCACTTGT GCTAGTAGAC TACAAAGTTA AAGCCTAGCT TCTGTATGCT
  481 TTTGGGGAAT ATCAGTTGAA ATGTTTGTAC TTGTCCAGAA ACAAAGTTCA CTTTGAAGTT
  541 CAGTCATCAC CTCCACCGTA CATGTCAGCA AGTTTCTTGA AACGTGGGCC CCAGTCATTT
  601 AGGTAGTCAT AGTCTTGCTC ACCACCGCTA CTTGAGGAAT TAAGAGAGCT CAAGGATCCA
  661 GCAGTGGAGC CGCTTCCTTC ATAGTCAAAG ACTAAGAGGG AATCGTATGG CGGGGCTGTA
  721 GGGTCGTTGT CGGCTGCTTT AAGTCCCTCA TTAATGAAGT CCCCAATGTC CCCAGGATGA
  781 GGAGCAGCTG ATCTGACTGG ATACTGAGGT TCTGCATGGA TTGGCCTTTC ATCAAGACGT
  841 CTGATTCCAA CAGGTTTGAT GGCGTCTGGT TCTACAGTGT CAGGCTGCTG GAGCTGGCTC
  901 AAGTCATAAA TCCTGATCTT CTTCTCCACC ACCTTCTTCA TCATATTTCA GAAATGTTGT
  961 CCCTCACATC ATCTTCTGGA TCAATTAAGA GCTGCTTGGC CCTGACGCTC CTTATCACGG
 1021 GCGCTTCATC CATACTACGA ACATCAAAAC TAAAATGAGT AAGATGATGA TACAAAGCCA
 1081 GATTGCAATG ATGGCACCAG TGCCAGTCTT GCGCAACAAT TCGGGTTCAA CATTCAGTAA
1141 CCAAGTTCCC CCAATTTATA TGTGGTTTAC
//

Please visit Sequencing and PCR primers for primer information.


References

Original, user report and related articles

original Hatta, K., Cloning and expression of cDNA encoding a neural calcium-dependent cell adhesion molecule: its identity in the cadherin gene family. J. Cell Biol., 106, 873- 881 (1988). PMID 2831236. [link to RRC of NBRP]