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Please review the QC test results indicated by check icon below as well as clone information before placing your order.

Mouse E-cadherin full length cDNA (#RDB01184)

Mouse E-cadherin cDNA.

Alternative name Mouse E-cadherin cDNA
Clone info. Mouse E-cadherin cDNA from F9 cells cDNA library.
Comment This clone contains a CDS fragment corresponding to the 69nt to 2723nt of X06115.1. Known differences (at least) : G to A substitution at 2714nt (Glu, synonym).
Vector backbone pBluescript (Plasmid)
Size of vector backbone 2.9 kb
Selectable markers Amp^r
Gene/insert name Mouse E-cadherin/Cdh1 cDNA
Reference of insert sequence X06115 (DDBJ accession number)
Depositor|Developer Takeichi, Masatoshi |
 
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Catalog # Resource name Shipping form Fee (non-profit org.)
RDB01184 Mouse E-cadherin full length cDNA DNA solution

Please review the QC test results indicated by check icon below as well as clone information before placing your order.

How to cite this biological resource

Materials & Methods section:

The Mouse E-cadherin full length cDNA was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB01184).

Reference section:

Nagafuchi, A., Shirayoshi, Y., Okazaki, K., Yasuda, K., Takeichi, M., Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA. Nature, 329, 341-343 (1987). PMID 3498123. [link to RRC of NBRP]

Further references such as user reports and related articles (go to bottom)

QC test results

RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by check icon as well as clone information before placing your order.

Test sheet RDB20024_B3Frp1-1.pdf check

Nucleotide sequence of a portion of this resource (if available).

Primer: pUC-M13_-100 (Pr0310)
Region: T7 pro,insert 5'
Sequence file: RDB20024_B3Fra.seq check
>01184_20024_B3Fr_2_pUC-M13_-100_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNG NNANGCCNGG GTTTTCCCAG TCACGACGTT GTAAAACGAC
   61 GGCCAGTGAA TTGTAATACG ACTCACTATA GGGCGAATTG GGTACCGGGC CCCCCCTCGA
  121 GGTCGACGGT ATCGATAAGC TTGATATCGA ATTCCTGCAG CCCCCTTACA ATTAATACAT
  181 AACCTTTTGT ATCATACACA TACGATTTAG GTGACACTAT AGAATTCGGG CCGGCCTAAC
  241 CCGGCCCTGC CCGACCGCAC CCGAGCTCAG TGTTTGCTCG GCGTCTGCCG GGTCCGCCAT
  301 GGGAGCCCGG TGCCGCAGCT TTTCCGCGCT CCTGCTCCTG CTGCAGGTCT CCTCATGGCT
  361 TTGCCAGGAG CTGGAGCCTG AGTCCTGCAG TCCCGGCTTC AGTTCCGAGG TCTACACCTT
  421 CCCGGTGCCG GAGAGGCACC TGGAGAGAGG CCATGTCCTG GGCAGAGTGA GATTTGAAGG
  481 ATGCACCGGC CGGCCAAGGA CAGCCTTCTT TTCGGAAGAC TCCCGATTCA AAGTGGCGAC
  541 AGACGGCACC ATNNNNNNGA AGCGGCATCT AAAGCTCCNN NANNCTNGAN ANCNNGNTTC
  601 CTCGTCCGNN NNNGGGNANT CNNNTCATNN NNAGCTGTCT ANNNNNNTGA CNNTGAAGTC
661 NATGNGNGCN NNNNCATCAC CGGNANNNNC ANNNCGNNCC CNGCNNCNNN NNNNNNN
//
Primer: reverse-2 (Pr0100)
Region: T3 pro,insert 3'
Sequence file: RDB20024_B3Frb.seq check
>01184_20024_B3Fr_2_reverse-2_D01_04_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNN TCGNNNNTAA CCCTCACTAA AGGGAACAAA AGCTGGAGCT
   61 CCACCGCGGT GGCGGCCGCT CTAGAACTAG TGGATCCCCC CGAGCTCGAA TTCGGGTTTT
  121 TTTACAGTTT AACAAAACTC TTTAATAAAA TTCATAAAAA TGCATCTTAG AGAACGGTTT
  181 CAATGGCTTA CCTTTTCCAC CCCCCACAAA ATACAAAATT ATCAGCACCC ACACACATAC
  241 ACTCAAACTA CATACAAAGG TCACTCTAGC AACATTACAT GATTAAACTA CAGAGAAATC
  301 AACAGTTCTC ACAGTTGATT GTGGTCATCA TTTTCTTCCA AAGTTAGGTA AGTACAGAAC
  361 TTTCAATAAA ATTCTTTCCA GTGGTTATCA AAAAGCTGAG ATTTGCATTT TGAATGAAAA
  421 CTCTCAAAGG TCTTGTCTCC TTTTTCCCGT TAGAATGTTC TCTTCCTGCT TCCTGANAAA
  481 ATGCACAATT TGTAATATTC TCAGTTTTCT CTTTTGGTTT GCANANACAG GGTTTCTCTG
  541 TGTANCCCTN NNTGTCCNAN AATTCGCTTA CNATACTATA TATACNNNNT TNGCCNCNAA
601 CTCNNGNANN NCTGANNGNC NNNGCCNNNN GANNGNTNGG CNNNAANNNN NNNNNN
//
Primer: E-Cad-B_R (Pr1332)
Region: insert mid
Sequence file: RDB20024_B3Frc.seq check
>01184_20024_B3Fr_2_E-Cad-B_R_E01_05_ABI08.ab1
    1 NNNNNNNNNN NNNNNNNNNT GNNNNCTTCT NNGATCAGTC TCTCTTCTAC NCACTCAGGG
   61 NAGGAGCTGA AAGACCACTG ATATAATTAT TCTGCATCTC CCATGGTGCC ACACGGGGGA
  121 GACTTGCTAG TCCCCTAGTC GTCTTCGCCA CCGCCGTACA TGTCCGCCAG CTTCTTGAAT
  181 CGGTTGCCCC ACTCGTTCAG ATAATCGTAG TCCTGGTCCT GATCCGACTC AGAGGAGTTC
  241 AGTGAGCTCA GGCTAGCGGC TTCAGAACCA CTGCCCTCGT AATCGAACAC CAACAGAGAG
  301 TCGTAAGGGG GTGCCGTGGG GTCGCTGTCG GCTGCCTTCA GGTTTTCATC GATGAAGTTT
  361 CCAATTTCAT CAGGATTGGC AGGACGGGGA CGATACTGGG GCACGCTCAT GAGGGTGGGA
  421 GCCACATCAT TTCGAGTCAC TTCCGGTCGG GCATCCAGGC CCCTGTGCAG CTGGCTCAAA
  481 TCAAAGTCCT GGTCTTCTTC TCCACCTCCT TCTTCATCAT AGTAATACAC ATTGTCCCGG
  541 GTATCATCAT CTGGTGGCAG CAGGGGCTCT TTGACCACCG TTCTCCTCCG TAGAAACAGT
  601 AGGAGCAGCA GGATCAGAAT CAGCAGGGCG AGGATCCCTC CAAGGATTCC GAGGATGGCA
  661 GGAACTTGCA ATCCTGCTGC CACGATTCCC GCCTTCATGC AGTTGTTGAC CGTCCCTTCA
  721 CAGTCACACA CATGGACGTC CAACGTGGTC ACCTGGTCTT TGTTCTGGTT ATCCGCGAGC
  781 TTGAGATGGA NTTTGTANTC NCCAATCTCT AAGTCCTTTC TTGGTTGCAA AATGAGAGAT
  841 TCTTGAGCTG CGTCATTATA CTCAATGGTC CANTTGANGC TGGNCCNATG GGTTAGCTCA
  901 NCANTAAAGG GGGNNGTGTN GGGGGGNANG NCTGGATNCN AGANGGNGAN GANATGANNN
  961 TGNGGGTTNN NNNNGNNANA NNNGNNTGNT TNNNNGNNNN NGGNNNNGNN NNNNTNNNNN
1021 NTNNNNNNNN NN
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Nagafuchi, A., Transformation of cell adhesion properties by exogenously introduced E-cadherin cDNA. Nature, 329, 341-343 (1987). PMID 3498123. [link to RRC of NBRP]
user_report Nag, K., E-Cadherin – Fc Chimeric Protein-Based Biomaterial: Breaking the Barriers in Stem Cell Technology and Regenerative Medicine. Advanced Materials Research, 810: 41-76 (2013). [link to RRC of NBRP]
user_report Jovic, D., Direct observation of cell cycle progression in living mouse embryonic stem cells on an extracellular matrix of E-cadherin. SpringerPlus, 2: 585 (2013). PMID 25674414. [link to RRC of NBRP]
user_report Meng, Q., The differentiation and isolation of mouse embryonic stem cells toward hepatocytes using galactose-carrying substrata. Biomaterials, 33 (5): 1414-1427 (2012). PMID 22118818. [link to RRC of NBRP]
user_report Jovic, D., Control of singular cell cycle synchronization of mouse ES cells for hepatocyte differentiation on E-cadherin substratum. J. Biotechnol. Biomaterial, 1: 6 (2011). [link to RRC of NBRP]
user_report Haque, A., The effect of recombinant E-cadherin substratum on the differentiation of endoderm-derived hepatocyte-like cells from embryonic stem cells. Biomaterials, 32, 2032-2042 (2011). PMID 21163520. [link to RRC of NBRP]
user_report Nagaoka M., Design of the artificial acellular feeder layer for the efficient propagation of mouse embryonic stem cells. J. Biol. Chem., 283, 26468-26476 (2008). PMID 18614540. [link to RRC of NBRP]
user_report Ito A., Enhancement of cell function through heterotypic cell-cell interactions using E-cadherin-expressing NIH3T3 cells. J. Biosci. Bioeng., 105, 679-682 (2008). PMID 18640611. [link to RRC of NBRP]
user_report Rosshart S., Interaction of KLRG1 with E-cadherin: new functional and structural insights. Eur. J. Immunol., 38, 3354-3364 (2008). PMID 19009530. [link to RRC of NBRP]
user_report Grundemann C., Cutting edge: identification of E-cadherin as a ligand for the murine killer cell lectin-like receptor G1. J. Immunol., 176, 1311-1315 (2006). PMID 16424155. [link to RRC of NBRP]
user_report Nagaoka M., E-cadherin-coated plates maintain pluripotent ES cells without colony formation. PLoS One, 1, (2006). PMID 17183641. [link to RRC of NBRP]
user_report Nagaoka M., Immobilized E-cadherin model can enhance cell attachment and differentiation of primary hepatocytes but not proliferation. Biotechnol. Lett., 24, 1857-1862 (2002). [link to RRC of NBRP]