A cassette cosmid for construction of recombinant adenovirus
Alternative name | pAdex1cw |
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Clone info. | A cassette cosmid for construction of recombinant adenovirus. This cosmid vector, which is a promoter-less vector, can be used to clone a gene driven by any promoter at the unique SwaI site in the E1 region. The pAxcw cosmid can be used to generate rAd by the COS-terminal protein complex (TPC) method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996]. |
Comment | The pAxcw cosmid [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996; Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995] is a 42.5-kbp charomid vector [Saito and Stark, Proc. Natl. Acad. Sci. USA 83, 8664-68, 1996] that includes the Ad5 genome from 0.01 to 99.3 map units (mus) with deletions of E1 (mu 1.3 to 9.3) and E3 (mu 79.6 to 84.8). |
Vector backbone | pAxcw (Cosmid, use packaging extracts for transforming E. coli host) |
Size of vector backbone | 42.5 kb |
Selectable markers | Amp^r |
Depositor|Developer | Saito, Izumu | |
Additional map | Remarks, protocol and/or map (pdf) | RDB00917.pdf |
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Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Ordering forms | Order form [Credit Card Payment] [Bank Transfer Payment] [Example of order form ] MTA, for use for not-for-profit academic purpose [Word] [Example of MTA ] Please visit Information of Request for Distribution.[link] For for-profit-research purpose, please contact us. |
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Terms and conditions for distribution | In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested. |
Remarks | Remember that you will be working with samples containing infectious virus. |
必要書類 | 提供依頼書 [依頼書の記入例 ] 提供同意書 (MTA, 非営利学術目的用)[Word] [MTAの記入例 ] 手続きの概要は、「提供申込みについて[link]」をご覧ください。営利目的利用についてはお問い合わせください。 |
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MTAに書く使用条件 | 利用者は、研究成果の公表にあたって寄託者の指定する文献を引用する。 |
備考 | このリソースはウイルス粒子産生用のため、取扱いに注意が必要です。 |
Catalog # | Resource name | Shipping form | Fee (non-profit org.) |
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RDB00917 | pAxcw | DNA solution |
Materials & Methods section:
The pAxcw was provided by the RIKEN BRC through the National BioResource Project of the MEXT, Japan (cat. RDB00917). |
Reference section:
Miyake, S., Makimura, M., Kanegae, Y., Harada, S., Sato, Y., Takamori, K., Tokuda, C., Saito, I., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762. [link to RRC of NBRP] |
Kanegae, Y., Lee, G., Sato, Y., Tanaka, M., Nakai, M., Sakaki, T., Sugano, S., Saito, I., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP] |
Further references such as user reports and related articles (go to bottom)
RIKEN BRC has sequenced portions of this material for quality test.
Please review the QC test results indicated by icon as well as clone information before placing your order.
Test sheet | RDB13739_A5Lmp1.pdf |
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Nucleotide sequence of a portion of this resource (if available).
Primer: Amp_R Sequence file: RDB13739_A5Lma.seq |
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>00917_13739_A5Lm_1_Amp_R_G04_19.ab1 1 CTTGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG 61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC 121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA 181 GGCCCTTTCG TCTTCAAGAA TTCCGGATCC GGGCCAATAT GATAATGAGG GGGTGGAGTT 241 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG 301 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG 361 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT 421 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA 481 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG 541 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC 601 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGCATCGAT TTAAATCGAT TGTCGACTCG 661 CGACGATGGA TCTGGAAGGT GCTGAGGTAC GATGAGACCC GCACCAGGTG CAGACCCTGC 721 GAGTGTGGCG GTAAACATAT TAGGAACCAG CCTGTGATGC TGGATGTGAC CGAGGAGCTG 781 AGGCCCGATC ACTTGGTGCT GGCCTGCACC CGCGCTGAGT TTGGCTCTAG CGATGAAGAT 841 ACAGATTGAG GTACTGAAAT GTGTGGGCGT GGCTTAAGGG TGGGAAAGAA TATATAAAGG 901 TGGGGGTCTT ATGTAGTTTT TGTATCTGTT TTGCAGCAGC CGCCGCCGCC ATGAGCACCA 961 ACTCGTTTGA TGGGAAGCAT TGTGAGCTCA TATTTTGACA ACGCGCATGC CCCCCATGGG 1021 GCGGGGGTGC GGTCAGAATG GTGATGGGGC GTCTCCAGCC ATTGAATGTG TGCGCCCCCG 1081 GTCCTTGTCC CGCCAAACAT TTACTTAACA TTGAACCTAA CAAGAAACAG TGGTTCTTGG 1141 GAACGCACGC TGGAGAACTT GCAGCTTCGT CGCCGCTTTC TAGTCGCTTG CCAGCCAACA 1201 GGACCCCGGG GGAATTGTGT GTGAACCT // |
Please visit Sequencing and PCR primers for primer information.
Original, user report and related articles
original | Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762. [link to RRC of NBRP] |
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original | Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022. [link to RRC of NBRP] |
user_report | Kanatsu-Shinohara, M., Adeno-associated-virus-mediated gene delivery to ovaries restores fertility in congenital infertile mice. Cell Rep. Med. 3 (5): 100606 (2022) PMID 35584625. [link to RRC of NBRP] |
user_report | Furukawa T., Potential tumor suppressive pathway involving DUSP6/MKP-3 in pancreatic cancer. Am. J. Pathol., 162, 1807-1815 (2003). PMID 12759238. [link to RRC of NBRP] |
user_report | Kojima H., Construction and characterization of adenoviral vector expressing biologically active brain-derived neurotrophic factor. Biochem. Biophys. Res. Commun., 212, 712-717 (1995). PMID 7542888. [link to RRC of NBRP] |
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