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pAxcw

A cassette cosmid for construction of recombinant adenovirus

Catalog number RDB00917
Resource name pAxcw
Alternative name pAdex1cw
Clone info. A cassette cosmid for construction of recombinant adenovirus. This cosmid vector, which is a promoter-less vector, can be used to clone a gene driven by any promoter at the unique SwaI site in the E1 region. The pAxcw cosmid can be used to generate rAd by the COS-terminal protein complex (TPC) method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
Comment The pAxcw cosmid [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996; Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995] is a 42.5-kbp charomid vector [Saito and Stark, Proc. Natl. Acad. Sci. USA 83, 8664-68, 1996] that includes the Ad5 genome from 0.01 to 99.3 map units (mus) with deletions of E1 (mu 1.3 to 9.3) and E3 (mu 79.6 to 84.8).
Vector backbone pAxcw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 42.5 kb
Selectable markers Amp^r
Depositor|Developer Saito, Izumu |
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            Reference sequence
              

            Distribution information

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            Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
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            Remarks Remember that you will be working with samples containing infectious virus.
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            RDB00917 pAxcw DNA solution

            Please review the QC test results indicated by check icon below as well as clone information before placing your order.

            References and tips

            Electronic file

            Remarks, protocol and/or map (gif) RDB00917.gif
            Remarks, protocol and/or map (pdf) RDB00917.pdf

            Original reference

            original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762.
            original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

            Further references such as user reports and related articles (go to bottom)

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            Sequence information

            RIKEN BRC has sequenced portions of this material for quality test.
            Please review the QC test results indicated by check icon as well as clone information before placing your order.

            Test sheet RDB13739_A5Lmp1.pdf check

            Nucleotide sequence of a portion of this resource (if available).

            Primer: Amp_R
            Sequence file: RDB13739_A5Lma.seq check
            >00917_13739_A5Lm_1_Amp_R_G04_19.ab1
                1 CTTGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
               61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
              121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
              181 GGCCCTTTCG TCTTCAAGAA TTCCGGATCC GGGCCAATAT GATAATGAGG GGGTGGAGTT
              241 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG
              301 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG
              361 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT
              421 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA
              481 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG
              541 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC
              601 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGCATCGAT TTAAATCGAT TGTCGACTCG
              661 CGACGATGGA TCTGGAAGGT GCTGAGGTAC GATGAGACCC GCACCAGGTG CAGACCCTGC
              721 GAGTGTGGCG GTAAACATAT TAGGAACCAG CCTGTGATGC TGGATGTGAC CGAGGAGCTG
              781 AGGCCCGATC ACTTGGTGCT GGCCTGCACC CGCGCTGAGT TTGGCTCTAG CGATGAAGAT
              841 ACAGATTGAG GTACTGAAAT GTGTGGGCGT GGCTTAAGGG TGGGAAAGAA TATATAAAGG
              901 TGGGGGTCTT ATGTAGTTTT TGTATCTGTT TTGCAGCAGC CGCCGCCGCC ATGAGCACCA
              961 ACTCGTTTGA TGGGAAGCAT TGTGAGCTCA TATTTTGACA ACGCGCATGC CCCCCATGGG
             1021 GCGGGGGTGC GGTCAGAATG GTGATGGGGC GTCTCCAGCC ATTGAATGTG TGCGCCCCCG
             1081 GTCCTTGTCC CGCCAAACAT TTACTTAACA TTGAACCTAA CAAGAAACAG TGGTTCTTGG
             1141 GAACGCACGC TGGAGAACTT GCAGCTTCGT CGCCGCTTTC TAGTCGCTTG CCAGCCAACA
            1201 GGACCCCGGG GGAATTGTGT GTGAACCT
            //

            Please visit Sequencing and PCR primers for primer information.


            References

            Original, user report and related articles

            original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762.
            original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
            user_report Furukawa T., Potential tumor suppressive pathway involving DUSP6/MKP-3 in pancreatic cancer. Am. J. Pathol., 162, 1807-1815 (2003). PMID 12759238.
            user_report Kojima H., Construction and characterization of adenoviral vector expressing biologically active brain-derived neurotrophic factor. Biochem. Biophys. Res. Commun., 212, 712-717 (1995). PMID 7542888.

            2022.05.18

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