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pAxcw

A cassette cosmid for construction of recombinant adenovirus

Catalog number RDB00917
Resource name pAxcw
Alternative name pAdex1cw
Clone info. A cassette cosmid for construction of recombinant adenovirus. This cosmid vector, which is a promoter-less vector, can be used to clone a gene driven by any promoter at the unique SwaI site in the E1 region. The pAxcw cosmid can be used to generate rAd by the COS-terminal protein complex (TPC) method [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996].
The pAxcw cosmid [Miyake et al., Proc. Natl. Acad. Sci. USA 93, 1320-24, 1996; Kanegae et al., Nucleic Acids Res. 23, 3816-21, 1995] is a 42.5-kbp charomid vector [Saito and Stark, Proc. Natl. Acad. Sci. USA 83, 8664-68, 1996] that includes the Ad5 genome from 0.01 to 99.3 map units (mus) with deletions of E1 (mu 1.3 to 9.3) and E3 (mu 79.6 to 84.8).
Vector backbone pAxcw (Cosmid, use packaging extracts for transformation)
Size of vector backbone 42.5 kb
Selectable markers Amp^r
Depositor Saito, Izumu |

Distribution information

Please check terms and conditions set forth by the depositor, which are specified in the RIKEN BRC Catalog and/or Web Catalog.
Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, a citation of literature(s) designated by the DEPOSITOR is requested.
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Remarks Remember that you will be working with samples containing infectious virus.
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RDB00917 pAxcw DNA solution

Ordering Information [in Japanese] [in English]

References and tips

Electronic file

Electronic file Map RDB00917.gif from Depositor
Electronic file Remarks, protocol and/or map (pdf) RDB00917.pdf from Depositor

Original reference

original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.

Further references such as user reports and related articles (go to bottom)

Featured content

Featured content Empty Backbone (English text)
Featured content Empty Backbone (Japanese text)

Sequence information

RIKEN BRC has sequenced portions of this material for quality test. Primers and the results are shown below.

Data are summerized on test sheet below.

Test sheet RDB13739_A5Lmp1.pdf

Nucleotide sequence of a portion of this resource (if available).

Primer: Amp_R | Sequence: RDB13739_A5Lma.seq
>00917_13739_A5Lm_1_Amp_R_G04_19.ab1
    1 CTTGTTTCAT ATTATTGAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG
   61 AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC
  121 CTGACGTCTA AGAAACCATT ATTATCATGA CATTAACCTA TAAAAATAGG CGTATCACGA
  181 GGCCCTTTCG TCTTCAAGAA TTCCGGATCC GGGCCAATAT GATAATGAGG GGGTGGAGTT
  241 TGTGACGTGG CGCGGGGCGT GGGAACGGGG CGGGTGACGT AGTAGTGTGG CGGAAGTGTG
  301 ATGTTGCAAG TGTGGCGGAA CACATGTAAG CGACGGATGT GGCAAAAGTG ACGTTTTTGG
  361 TGTGCGCCGG TGTACACAGG AAGTGACAAT TTTCGCGCGG TTTTAGGCGG ATGTTGTAGT
  421 AAATTTGGGC GTAACCGAGT AAGATTTGGC CATTTTCGCG GGAAAACTGA ATAAGAGGAA
  481 GTGAAATCTG AATAATTTTG TGTTACTCAT AGCGCGTAAT ATTTGTCTAG GGCCGCGGGG
  541 ACTTTGACCG TTTACGTGGA GACTCGCCCA GGTGTTTTTC TCAGGTGTTT TCCGCGTTCC
  601 GGGTCAAAGT TGGCGTTTTA TTATTATAGT CAGCATCGAT TTAAATCGAT TGTCGACTCG
  661 CGACGATGGA TCTGGAAGGT GCTGAGGTAC GATGAGACCC GCACCAGGTG CAGACCCTGC
  721 GAGTGTGGCG GTAAACATAT TAGGAACCAG CCTGTGATGC TGGATGTGAC CGAGGAGCTG
  781 AGGCCCGATC ACTTGGTGCT GGCCTGCACC CGCGCTGAGT TTGGCTCTAG CGATGAAGAT
  841 ACAGATTGAG GTACTGAAAT GTGTGGGCGT GGCTTAAGGG TGGGAAAGAA TATATAAAGG
  901 TGGGGGTCTT ATGTAGTTTT TGTATCTGTT TTGCAGCAGC CGCCGCCGCC ATGAGCACCA
  961 ACTCGTTTGA TGGGAAGCAT TGTGAGCTCA TATTTTGACA ACGCGCATGC CCCCCATGGG
 1021 GCGGGGGTGC GGTCAGAATG GTGATGGGGC GTCTCCAGCC ATTGAATGTG TGCGCCCCCG
 1081 GTCCTTGTCC CGCCAAACAT TTACTTAACA TTGAACCTAA CAAGAAACAG TGGTTCTTGG
 1141 GAACGCACGC TGGAGAACTT GCAGCTTCGT CGCCGCTTTC TAGTCGCTTG CCAGCCAACA
1201 GGACCCCGGG GGAATTGTGT GTGAACCT
//

Please visit Sequencing and PCR primers for primer information.

References

Original, user report and related articles

original Miyake, S., Efficient generation of recombinant adenoviruses using adenovirus DNA-terminal protein complex and a cosmid bearing the full-length virus genome. Proc. Natl. Acad. Sci. USA, 93, 1320-1324 (1996). PMID 8577762.
original Kanegae, Y., Efficient gene activation in mammalian cells by using recombinant adenovirus expressing site-specific Cre recombinase. Nucleic Acids Res., 23, 3816-3821 (1995). PMID 7479022.
user report Furukawa T., Potential tumor suppressive pathway involving DUSP6/MKP-3 in pancreatic cancer. Am. J. Pathol., 162, 1807-1815 (2003). PMID 12759238.
user report Kojima H., Construction and characterization of adenoviral vector expressing biologically active brain-derived neurotrophic factor. Biochem. Biophys. Res. Commun., 212, 712-717 (1995). PMID 7542888.

2019.10.05

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