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Sulfolobus tokodaii Protein Expression Plasmid - StoEx08A10

 
Catalog Number THR043210
Clone Name StoEx08A10
Vector pET21a
Plasmid ID of the Whole Cell Project ST0077
Description Bacterial expression plasmid clone of Sulfolobus tokodaii STK_RS00415.
536aa long hypothetical fatty-acid--CoA ligase
Note ST0077
Depositor Kawarabayasi, Yutaka
 
Source Sulfolobus tokodaii strain7
Locus symbol ST0077
Synonym -
NCBI locus tag ST0077
Protein Name fatty acid--CoA ligase
NCBI Gene ID 1457958
Other clones
KEGG gene STK_00770
Links

  KEGG gene

  NCBI Gene

Distribution information

Ordering information ordering information

Terms and conditions set forth by the DEPOSITOR In publishing the research results obtained by use of the BIOLOGICAL RESOURCE, the USER is expected to cite the literature specified by the DEPOSITOR.
 
Catalog # Clone name Shipping form Fee (non-profit org.)
THR043210 StoEx08A10 DNA solution JPY 9,460 plus cost of shipping containers, dry ice (if required) and shipping charge

Status and protocol provided by the depositor.

Expressed Soluble Purified Protocol
yes      

This clone has been sequenced a portion for verification.

Seq File Sto08A10a.seq
>StoEx08A10 (THR043210) 5' side
    1 GNNTGCGGAC ATTCCCTCTG AATATTTTGT TTACTTTAAG AAGGAGATAT ACATATGAGT
   61 GAATATTACG AATATGAATT AACAATAGAT AAGATTCTAG ATACTGGAGT GAAAAGCTTC
  121 CCAGATAGAG AAATAGTATA TAGGGATGTT AGAAGGTATA CTTTTTCATC TTTTGCAGAT
  181 TCTGTAAGAA GGTTAATGAG TGGGTTAAAG AAACTAGGTG TTAAAGAGGG CGAGACTGTA
  241 GGTGTTATAG ATTGGGATAC TGATGTCTAC CTTCATTCTT ACTATGCTAT TCCTATGTTG
  301 GGTTCTGTTT TGCATACAGT TAATATAAGA TACCCACCAG AAATAATATT GAAGACTATA
  361 TTACAAGCTG AAGATAAATA TTTGATCGTT AGGGACGAGT TTTTACCCTT ATTAGAGAAA
  421 GCCAAGAATC TTCTTCCAGT GGGAATGAAG ATTATAACAT ATAGTGATAG CAAAGAGAAA
  481 GTAAGGTCTA ATATTTCTGA TGCAGATTTT TGGGAGTTGA TTGATAGTAG TGAGCCTTCA
  541 GAAATACCAC AAGTTAGTGA AAATAGTAGA GCTACTATAT TCTTTACTTC TGGAACTACT
  601 GGTGACCCTA AAGGTGTTTG GTTTACGCAT AGAAAACTTG TTCTTCACGC GTTAAGCGTA
  661 AGCTTAGTAG GTGCGAGACC ACCTTTAAAC GTAACAACTA ATGACGTTTA CTTAATATTG
  721 GTTCCCCATG TTTCATGTAC ATTCTTGGGG ATATCCCTAC GTGTTTATGT TGTCTGGAAT
  781 TAAATATGTT TTACCGGGGA AGTATGATTA CNGTCTTATA CTAAAACTTA TGGATAAGGG
841 AAAGAGTTAC CTNCTCGGCT ATGGNACCNA CNATATN
//

References and tips

References

reference Kawarabayasi, Y. Complete genome sequence of an aerobic thermoacidophilic crenarchaeon, Sulfolobus tokodaii strain7. DNA Res. 8: 123-140 (2001). PMID 11572479

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