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Plasmid & Protein Purification Database -Purification-

Species  
Locus tag STS186
Products  
Plasmid ID 0490
Vector  
Purification Lot STS186_1-5
Purified Date  
Protein conc. (mg/ml) 4.86
Total volume (ml) 0.45
Total protein (mg) 2.18

Culture Lot:

date host cell scale label weight of bacteira (g)
        34.5

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS 1.5M AS 0M AS 6 1.2M AS
2 Resource Iso 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,2.0M AS 2.0M AS 0M AS 6 1.7M AS
3 Resource Q 6 ml 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 2M NaCl 6 Flow through
4 Bio-Scale CHT5I 10mM KPi(pH7.0),5mM B-ME 10mM KPi 500mM KPi 2 48.7mM KPi
5 HiLoad 16/60 Superdex 75 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     1 80min
6            
7            
8            
9            

Comment:

At the end of purification 1, solution was divided into five fractions, and the purified individually.

2011.10.20

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