Species | |
---|---|
Locus tag | STS186 |
Products | |
Plasmid ID | 0490 |
Vector | |
Purification Lot | STS186_1-5 |
Purified Date | |
Protein conc. (mg/ml) | 4.86 |
Total volume (ml) | 0.45 |
Total protein (mg) | 2.18 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
34.5 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Phe 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS | 1.5M AS | 0M AS | 6 | 1.2M AS |
2 | Resource Iso 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,2.0M AS | 2.0M AS | 0M AS | 6 | 1.7M AS |
3 | Resource Q 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 2M NaCl | 6 | Flow through |
4 | Bio-Scale CHT5I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 500mM KPi | 2 | 48.7mM KPi |
5 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 1 | 80min | ||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
At the end of purification 1, solution was divided into five fractions, and the purified individually. |
2011.10.20
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