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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag STS140
Products hypothetical glutamyl-tRNA (gln) amidotransferase subunit C
Plasmid ID 0291
Vector  
Purification Lot STS140
Purified Date 2004.7.5-8.18
Protein conc. (mg/ml) 18.9
Total volume (ml) 1.35
Total protein (mg) 25.5

Culture Lot:

date host cell scale label weight of bacteira (g)
        28.3

Cell Lysis and Heat Treatment:

 
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Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 BioAssist Q 10x10 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 1M NaCl 3 0.07M NaCl
2 HiTrap Heparin HP 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 2M NaCl 1 0.12M NaCl
3 HiPrep 26/10 Desalting 53ml 20mM Tris-HCl(pH8.0),5mM B-ME        
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Comment:

Products was confirmed by MS.

2011.10.20

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