Species | |
---|---|
Locus tag | STS042 |
Products | |
Plasmid ID | 0155 |
Vector | |
Purification Lot | STS042_3b |
Purified Date | 2005.5.13-5.24 |
Protein conc. (mg/ml) | 11.8 |
Total volume (ml) | 0.15 |
Total protein (mg) | 1.77 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
19.6 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Phe 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,2.0M AS | 2.0M AS | 0M AS | 6 | from 1.93M AS to 1.58M AS |
2 | BioAssist Q 10x10 | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 0.25M NaCl | 2 | from 0.02M NaCl to 0.04M NaCl |
3 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 1 | 78ml/120ml | ||
4 | ||||||
5 | ||||||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
Products were devided in the gel filtration step into two peaks, the main peak (a, possibly multimer) and the sub peak (b, possibly monomer). Refer the previous purification lot. |
2011.10.20
- kuramitsuxxxx_html.pl