| Species | Sulfolobus tokodaii |
|---|---|
| Locus tag | ST2477 |
| Products | hypothetical NADP-dependent glyceraldehyde-3-phosphate dehydrogenase |
| Plasmid ID | 0573 |
| Vector | |
| Purification Lot | ST2477_2 |
| Purified Date | 2004.5.14 |
| Protein conc. (mg/ml) | 12.2 |
| Total volume (ml) | 0.98 |
| Total protein (mg) | 12 |
| date | host cell | scale | label | weight of bacteira (g) |
|---|---|---|---|---|
| 20 |
|
| Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
|---|---|---|---|---|---|---|
| 1 | Resource Q 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 0.8M NaCl | 5 | flow through & 160mM NaCl |
| 2 | Resource Iso 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS | 1.5M AS | 0M AS | 5 | 660mM AS |
| 3 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 500mM KPi | 3 | 120mM KPi |
| 4 | BioAssist Q 10x10 | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 0.6M NaCl | 2.5 | 240mM NaCl |
| 5 | HiLoad 16/60 Superdex 75 | 1 | ||||
| 6 | ||||||
| 7 | ||||||
| 8 | ||||||
| 9 |
2011.10.20
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