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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST2477
Products hypothetical NADP-dependent glyceraldehyde-3-phosphate dehydrogenase
Plasmid ID 0573
Vector  
Purification Lot ST2477_1
Purified Date 2004.5.14
Protein conc. (mg/ml) 14.7
Total volume (ml) 0.36
Total protein (mg) 5.3

Culture Lot:

date host cell scale label weight of bacteira (g)
        19

Cell Lysis and Heat Treatment:

19 g of E. coli.
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Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Q 6 ml 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 0.8M NaCl 5 flow through & 160mM NaCl
2 Bio-Scale CHT10I 10mM KPi(pH7.0),5mM B-ME 10mM KPi 500mM KPi 3 120mM KPi
3 HiTrap Blue HP 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 2M NaCl 5 Flow through
4 BioAssist Q 10x10 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 0.6M NaCl 2.5 240mM NaCl
5 Resource Iso 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS 1.5M AS 0M AS 5 660mM AS
6 Resource Q 6 ml 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 0.6M NaCl 5 170mM NaCl
7 BioAssist Q 10x10170 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 0.6M NaCl 2.5 240mM NaCl
8 HiLoad 16/60 Superdex 75 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     1  
9            

Comment:

Products was confirmed by MS.

2011.10.20

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