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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST2405
Products conserved hypothetical protein
Plasmid ID R129
Vector  
Purification Lot ST2405
Purified Date 2005.1.18-2.1
Protein conc. (mg/ml) 5.28
Total volume (ml) 1.78
Total protein (mg) 9.40

Culture Lot:

date host cell scale label weight of bacteira (g)
        43.6

Cell Lysis and Heat Treatment:

43.6 g of E. coli.
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Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,1.2M AS 1.2M AS 0M AS 6 1.06M AS
2 Resource S 6 ml 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 1M NaCl 6 0.440M NaCl
3 HiLoad 16/60 Superdex 75 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     0.7  
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Comment:

Products was confirmed by MS. Products shows blackish yellow. It was difficult to produce concentrated products.

2011.10.20

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