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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST2172
Products dihydroxy-acid dehydratase
Plasmid ID 119-2
Vector  
Purification Lot ST2172_2b
Purified Date 2004.12.7-12.21
Protein conc. (mg/ml) 12.2
Total volume (ml) 4.0
Total protein (mg) 48.8

Culture Lot:

date host cell scale label weight of bacteira (g)
        57.4

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,1.35M AS 1.35M AS 0M AS 6 1.1M AS
2 Resource Q 6 ml 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 2M NaCl 6 136mM NaCl
3 Bio-Scale CHT10I 10mM KPi(pH7.0),5mM B-ME 10mM KPi 500mM KPi 2 90.85mM KPi
4 HiLoad 16/60 Superdex75 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     1  
5            
6            
7            
8            
9            

Comment:

Products were confirmed by MS.

2011.10.20

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