Species | |
---|---|
Locus tag | ST2172 |
Products | |
Plasmid ID | 119 |
Vector | |
Purification Lot | ST2172_1a |
Purified Date | 2004.10.21-12.7 |
Protein conc. (mg/ml) | 15.5 |
Total volume (ml) | 0.15 |
Total protein (mg) | 2.33 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
22.8 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Phe 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.35M AS | 1.35M AS | 0M AS | 6 | 0.805M AS |
2 | Resource Q 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 2M NaCl | 6 | 146mM NaCl |
3 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 500mM KPi | 2 | 44.3mM KPi |
4 | HiLoad 16/60 Superdex200 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 1 | 66min | ||
5 | ||||||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
Products were confirmed by MS. At the end of the 1st purification step, two peaks were observed at 805 mM AS and 0 mM AS. Each of them were subjected by an ion exchange column at the 2nd step. At the end of 3rd step, the former peak was divided into two (a and e) and the later peak was divided into three (b, c and d). They were subjected, respectively, to a gel purification column at the next step of purification. |
2011.10.20
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