Species | |
---|---|
Locus tag | ST2055 |
Products | |
Plasmid ID | R254 |
Vector | |
Purification Lot | ST2055_5 |
Purified Date | 2005.6.20-6.30 |
Protein conc. (mg/ml) | 11.0 |
Total volume (ml) | 0.89 |
Total protein (mg) | 9.79 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
60.0 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Phe 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.2M AS | 1.2M AS | 0M AS | 6 | Flow through |
2 | Resource Phe 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.8M AS | 1.8M AS | 0M AS | 6 | 1.7M AS |
3 | Resource Q 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 2M NaCl | 4 | Flow through |
4 | HiPrep16/10 Heparin FF | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 2M NaCl | 2 | Flow through |
5 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 1 | |||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
2011.10.20
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