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Plasmid & Protein Purification Database -Purification-

Species  
Locus tag ST2055
Products  
Plasmid ID R254
Vector  
Purification Lot ST2055_3
Purified Date 2005.4.22-4.28
Protein conc. (mg/ml)  
Total volume (ml)  
Total protein (mg)  

Culture Lot:

date host cell scale label weight of bacteira (g)
        37.0

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Phe 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,2.0M AS 2.0M AS 0M AS 6  
2 Mono Q HR10x10 20mM Tris-HCl(pH8.0),5mM B-ME 0mM NaCl 500mM NaCl 3  
3 Resource S 6 ml 50mM MES(pH6.0),5mM B-ME 0M NaCl 1M NaCl 4  
4 Bio-Scale CHT10I 10mM KPi(pH7.0),5mM B-ME 10mM KPi 500mM KPi 2  
5 HiLoad 16/60 Superdex 75 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl        
6            
7            
8            
9            

Comment:

Products was confirmed by MS.

2011.10.20

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