Species | |
---|---|
Locus tag | ST2055 |
Products | |
Plasmid ID | R254 |
Vector | |
Purification Lot | ST2055_3 |
Purified Date | 2005.4.22-4.28 |
Protein conc. (mg/ml) | |
Total volume (ml) | |
Total protein (mg) |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
37.0 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Phe 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,2.0M AS | 2.0M AS | 0M AS | 6 | |
2 | Mono Q HR10x10 | 20mM Tris-HCl(pH8.0),5mM B-ME | 0mM NaCl | 500mM NaCl | 3 | |
3 | Resource S 6 ml | 50mM MES(pH6.0),5mM B-ME | 0M NaCl | 1M NaCl | 4 | |
4 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 500mM KPi | 2 | |
5 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | ||||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
Products was confirmed by MS. |
2011.10.20
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