| Species | |
|---|---|
| Locus tag | ST2055 |
| Products | |
| Plasmid ID | R254 |
| Vector | |
| Purification Lot | ST2055_2 |
| Purified Date | 2005.4.12- |
| Protein conc. (mg/ml) | |
| Total volume (ml) | |
| Total protein (mg) |
| date | host cell | scale | label | weight of bacteira (g) |
|---|---|---|---|---|
| 36.2 |
|
| Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
|---|---|---|---|---|---|---|
| 1 | Resource Phe 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.2M AS | 2.0M AS | 0M AS | ||
| 2 | Mono Q HR10x10 | 20mM Tris-HCl(pH8.0),5mM B-ME | ||||
| 3 | Mono S | 50mM MES(pH6.0),5mM B-ME | ||||
| 4 | Resource S 6 ml | |||||
| 5 | ||||||
| 6 | ||||||
| 7 | ||||||
| 8 | ||||||
| 9 |
| Products was confirmed by MS. |
2011.10.20
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