| Species | Sulfolobus tokodaii |
|---|---|
| Locus tag | ST2023 |
| Products | fumarate hydratase |
| Plasmid ID | 372 |
| Vector | |
| Purification Lot | ST2023_2 |
| Purified Date | 2004.4.13-6.9 |
| Protein conc. (mg/ml) | 14.1 |
| Total volume (ml) | 1.5 |
| Total protein (mg) | 21.15 |
| date | host cell | scale | label | weight of bacteira (g) |
|---|---|---|---|---|
| 27 |
|
| Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
|---|---|---|---|---|---|---|
| 1 | Resource Phe 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS | 1.5M AS | 0M AS | 6 | 0.2M AS |
| 2 | Resource Iso 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS | 1.5M AS | 1.5M AS | 6 | 0.53M AS |
| 3 | Resource Q 6 ml | 20mM Tris-HCl(pH8.0),5mM B-ME | 0M NaCl | 1M NaCl | 6 | 50mM NaCl |
| 4 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 1M KPi | 3 | 0.19M KPi |
| 5 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl | 0.5 | 104min | ||
| 6 | ||||||
| 7 | ||||||
| 8 | ||||||
| 9 |
| Products was confirmed by MS. |
2011.10.20
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