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Plasmid & Protein Purification Database -Purification-

Species Sulfolobus tokodaii
Locus tag ST2023
Products fumarate hydratase
Plasmid ID R0373
Vector  
Purification Lot ST2023_1
Purified Date 2003.12.11-
Protein conc. (mg/ml) 7.14
Total volume (ml) 1
Total protein (mg) 7.14

Culture Lot:

date host cell scale label weight of bacteira (g)
         

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource S 6 ml 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS 1.5M AS 0M AS 6 from 1.40M AS to 1.5M AS
2 Resource Q 6 ml 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 1M NaCl 6 from 0.05M NaCl to 0.1M NaCl
3 HiLoad 16/60 Superdex 75 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     0.5 96min
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Comment:

 

2011.10.20

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