Species | |
---|---|
Locus tag | ST1970 |
Products | |
Plasmid ID | 0249 |
Vector | |
Purification Lot | ST1970_1b |
Purified Date | 2005.1.4-1.19 |
Protein conc. (mg/ml) | 0.17 |
Total volume (ml) | 1.35 |
Total protein (mg) | 0.23 |
date | host cell | scale | label | weight of bacteira (g) |
---|---|---|---|---|
37.9 |
|
Step | Column | Buffer | Gradient 1 | Gradient 2 | FlowRate (ml/min) | Eluted |
---|---|---|---|---|---|---|
1 | Resource Phe 6ml | 20mM Tris-HCl(pH8.0),5mM B-ME,1.05M AS | 1.05M AS | 0M AS | 6 | Flow through |
2 | Resource S 6 ml | 50mM MES(pH6.0),5mM B-ME | 0M NaCl | 2M NaCl | 6 | Flow through |
3 | Bio-Scale CHT10I | 10mM KPi(pH7.0),5mM B-ME | 10mM KPi | 500mM KPi | 2 | 135mM KPi |
4 | Resource Q 6 ml | 20mM Tris-HCl(pH8.6),5mM B-ME | 0M NaCl | 2M NaCl | 6 | 112mM NaCl |
5 | HiLoad 16/60 Superdex 75 | 20mM Tris-HCl(pH8.6),5mM B-ME,150mM NaCl | 1 | 60min | ||
6 | ||||||
7 | ||||||
8 | ||||||
9 |
Products were confirmed by MS. |
2011.10.20
- kuramitsuxxxx_html.pl