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Available from Riken BioResource Center.

Plasmid & Protein Purification Database -Purification-

Species  
Locus tag ST1879
Products  
Plasmid ID R0247
Vector  
Purification Lot ST1879
Purified Date 2006.11.28-12.5
Protein conc. (mg/ml) 45.62
Total volume (ml) 1.65
Total protein (mg) 75.27

Culture Lot:

date host cell scale label weight of bacteira (g)
        28.5

Cell Lysis and Heat Treatment:

28.5 g of E. coli.
140 ml of 20mM Tris-HCl (pH8.0), 5mM B-ME, 50mM NaCl.
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1 Resource Iso 6ml 20mM Tris-HCl(pH8.0),5mM B-ME,1.5M AS 1.5M AS 0M AS 4 1.20M AS
2 Resource Q 6 ml 20mM Tris-HCl(pH8.0),5mM B-ME 0M NaCl 0.6M NaCl 5 0.27M NaCl
3 Bio-Scale CHT5I 10mM KPi(pH7.0),5mM B-ME 10mM KPi 300mM KPi 2 117mM KPi
4 HiLoad 16/60 Superdex 75 20mM Tris-HCl(pH8.0),5mM B-ME,150mM NaCl     1 68ml/120ml
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Comment:

Products were confirmed by Trpsin-MS. They shows yellow color.

2011.10.20

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