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Plasmid & Protein Purification Database -Purification-

Species  
Locus tag ST1818
Products  
Plasmid ID BL0244
Vector  
Purification Lot ST1818
Purified Date 2007.1.26-
Protein conc. (mg/ml)  
Total volume (ml)  
Total protein (mg)  

Culture Lot:

date host cell scale label weight of bacteira (g)
        24.5

Cell Lysis and Heat Treatment:

 
 
 
 
 
 
 
 
 

Purification Procedure:

Step Column Buffer Gradient 1 Gradient 2 FlowRate (ml/min) Eluted
1            
2            
3            
4            
5            
6            
7            
8            
9            

Comment:

Unable to be purified. There was almost no products in the supernatant of cell lysate and the producs was not observed after purification by a hydrophobicity interaction column. Products were unable to be extracted form the pellete after sonication in an MES buffer (pH 6.0).

2011.10.20

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